A5026537366- Renal Diseases and Glomerulopathies
- Cancer-related molecular mechanisms research
- Traditional Chinese Medicine Studies
- Autopsy Techniques and Outcomes
- Lipid metabolism and disorders
- Inflammasome and immune disorders
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Chronic Kidney Disease and Diabetes
- Pharmacological Effects of Natural Compounds
- Ginseng Biological Effects and Applications
- Traditional Chinese Medicine Analysis
- Neurological Disease Mechanisms and Treatments
- Connexins and lens biology
- Endoplasmic Reticulum Stress and Disease
- Traumatic Brain Injury Research
- Genetic and Kidney Cyst Diseases
- Acupuncture Treatment Research Studies
- Bioactive Compounds and Antitumor Agents
- Forensic Anthropology and Bioarchaeology Studies
- Natural product bioactivities and synthesis
- Calpain Protease Function and Regulation
- Nutrition, Genetics, and Disease
- Medical Research and Treatments
- Moringa oleifera research and applications
Nanfang Hospital
2024
Southern Medical University
2024
Capital Medical University
2012-2023
Changchun Bureau of Science and Technology
2021-2023
Beijing Fengtai Hospital
2021
Beijing University of Chinese Medicine
2009-2013
Peng Huanwu Center for Fundamental Theory
2012
Northwest A&F University
2009
New data indicate that abnormal glomerular endothelial cell (GEC)-podocyte crosstalk plays a critical role in diabetic nephropathy (DN). The aim of our study is to investigate the exosomes from high glucose (HG)-treated GECs epithelial-mesenchymal transition (EMT) and dysfunction podocytes. In this study, were extracted GEC culture supernatants podocytes incubated with GEC-derived exosomes. Here, we demonstrate HG induces endothelial-mesenchymal (EndoMT) HG-treated cells undergoing EndoMT...
Background: Endoplasmic reticulum stress is associated with podocyte apoptosis in the pathogenesis of diabetic nephropathy (DN). A previous study has demonstrated that emodin a protective effect kidney by suppressing proliferation mesangial cells and inhibiting renal tubular epithelial-to-mesenchymal transition. However, effects on DN its mechanisms are unknown. Aim: This aimed to explore model KK-Ay mice high glucose induced podocytes via PERK–eIF2α pathway. Methods: were treated at dose 40...
Podocyte dedifferentiation and mesangial cell (MC) activation play an important role in many glomerular diseases associated with fibrosis. MicroRNA-21 (miR-21) is closely linked to renal fibrosis, but it unknown whether how miR-21 promotes podocyte MC astragaloside IV (AS-IV) improves function fibrosis through the regulation of miR-21.Cultured MCs, primary mouse podocytes, diabetic KK-Ay mice were treated AS-IV. Cell transfection, Western blot, real-time PCR, immunofluorescence assay,...
Objective. Deficiencies in klotho are implicated various kidney dysfunctions including diabetic nephropathy (DN) related to inflammatory responses. Klotho is closely responses and a potential target for ameliorating failure. Pyroptosis, an form of programmed cell death, reported take part DN pathogenesis recently. This study aimed at exploring whether how inhibited podocyte pyroptosis astragaloside IV (AS-IV) protect through the regulation klotho. Materials Methods. SD rat model...
Astragaloside IV represses high glucose-induced mesangial cells activation by enhancing autophagy via SIRT1 deacetylation of NF-kappaB P65 subunit Xiaolei Wang,1,2 Yanbin Gao,1,2 Nianxiu Tian,1 Zhiyao Zhu,2 Tao Wang,1 Jiayi Xu,1 Bingjie Wu,1 Nan Zhang2 1Department Endocrinology, School Traditional Chinese Medicine, Capital Medical University, Beijing, China; 2Department Beijing Key Lab Medicine Collateral Disease theory Research, China Aim: Mesangial cell (MC) plays an important role in many...
Tongxinluo (TXL) is one of the most common traditional Chinese medicines and plays a vital role in treating atherosclerosis (AS). Endothelial cell (EC) pyroptosis crucial development AS. Previous research revealed inhibitory function TXL EC apoptosis autophagy. However, whether can inhibit ECs has not been determined.To explore influence on determine its underlying mechanism action AS.The components were determined by ultra-performance liquid chromatography coupled with photodiode array...
Prolonged hyperglycemia-induced oxidative stress and endoplasmic reticulum have been demonstrated to play a key role in progression of diabetic peripheral neuropathy (DPN). PERK/ Nrf2 pathway plays predominant (ER) which is associated with cell survival. This study examined the modulation PERK/Nrf2 apoptosis by traditional Chinese medicine Tangluoning (TLN) streptozotocin-induced DPN rat models effects serum TLN on pathway, apoptosis, intracellular reactive oxygen species mitochondrial...
Diabetic nephropathy (DN) is one of the most important diabetic microangiopathies. The epithelial-to-mesenchymal transition (EMT) plays an role in DN. physiological microRNA-21 (miR-21) was closely linked to EMT. However, it remained elusive whether tongxinluo (TXL) ameliorated renal structure and function by regulating miR-21-induced EMT This study aimed determine effect TXL on tubular explore relationship between miR-21 TGF-β1/smads signals. Real-time RT-PCR, cell transfection, situ...
MiRNAs play important roles in initiation and progress of many pathologic processes. MiR-21 was closely associated with diabetic nephropathy (DN). However, whether serum miR-21 as a potential diagnostic biomarker for DN the relationship between tissue remained unclear. In this study, real-time RT-PCR, cell transfection, luciferase reporter gene assays, western blot confocal microscope were used, respectively. Here, we found that renal significantly elevated DN. Moreover, assays showed smad7...
Insulin resistance (IR) plays a major role in the pathogenesis of abdominal obesity, hypertension, coronary heart disease, atherosclerosis and diabetes. miR-21 TGF-β/smads is closely related to IR. However, it remained elusive whether metformin improved skeletal muscle insulin (IRSM) by regulating its target signal TGF-β1/smads expression. In this study, high-fat diet rats with IR model IR-skeletal L6 cells (L6-SMCs) were established, sensitive index (ISI) Homeostasis assessment (HOMA-IR)...
Acute-on-chronic liver failure (ACLF) is a critical disease with high fatality rate. Immune dysfunction and inflammatory responses are key risk factors in ACLF. Pyroptosis form of programmed cell death characterized by the release cytokines, which causes strong responses. High mobility group box-1 (HMGB1) could induce pyroptosis closely related to However, role HMGB1-induced hepatocyte ACLF has never been proposed; whether participates development mechanisms involved barely understood.This...
To identify a safe and effective Impaired Glucose Tolerance (IGT) intervention program using Traditional Chinese Medicine (TCM) supported by Standard Health Care Advice (SHCA) for the evidence-based TCM in IGT prevention of type 2 diabetes. A total 510 patients were randomly assigned into either control or group (255 each group). The received standard health care according to SHCA. also addition care. study was conducted over three-year follow-up. At end three years follow-up, accumulative...
Microinflammation plays a crucial role in podocyte dysfunction diabetic nephropathy, but its regulatory mechanism is still unclear. This study aimed at discussing the mechanisms underlying effect of miRNA-155 on injury to determine potential as therapeutic target. Cultured immortalized mouse podocytes and KK-Ay mice models were treated with miR-155 inhibitor. Western blotting, real-time PCR, ELISA, immunofluorescence, Luciferase reporter assay used analyze markers inflammation cytokines...
Diabetic nephropathy (DN) is a progressive microvascular complication of diabetes mellitus (DM), driven largely by podocyte apoptosis. The cysteine protease Calpain 10 known to augment apoptosis and necrosis, potential therapeutic target in DN.Type 2 was induced SD rats high-fat diet (HFD) feeding streptozotocin (STZ) injections, simulated vitro culturing conditionally immortalized mouse podocytes hyperlipidemic (PA, 100 μM) conditions. rate the renal tissues cultured determined TUNEL assay....
Diabetic nephropathy (DN) is a major cause of chronic kidney failure and characterized by interstitial glomeruli fibrosis. Epithelial-to-mesenchymal transition (EMT) plays an important role in the pathogenesis DN. Tong xinluo (TXL), Chinese herbal compound, has been used China with established therapeutic efficacy patients To investigate molecular mechanism TXL improving DN, KK-Ay mice were selected as models for evaluation treatment In vitro, TGF- β 1 was to induce EMT. Western blot (WB),...
This experiment was undertaken to evaluate the effect of dietary vitamin A on performance and immune competence broilers under heat stress (HS). total 180 birds, at 22 days age, were randomly assigned be reared either 24°C (thermoneutral, TN, 24°C, constant) or 38°C (heat stress, HS, cycling) until age 42 days. Birds then supplemented with 750, 1500, 15 000 IU/kg. Each 2 × 3 factorially arranged treatments replicated in six cages, each containing five birds. Humoral immunity assessed by...
To diagnose coronary artery stenosis by using the postmortem computed tomography angiography (PMCTA), and to explore diagnostic value of PMCTA in sudden cardiac death.
Background. Diabetic nephropathy (DN) has become one of the leading causes end-stage renal disease (ESRD). Tang-Shen-Ning (TSN) decoction, an effective Traditional Chinese formula for DN, can improve function and inhibit fibrosis in DN. However, its potential mechanism is still unexplored. Methods. A network pharmacology approach was employed this study, including screening differential expressed genes DN (DN-DEGs), protein-protein interaction (PPI) analysis, GO KEGG enrichment analysis....