A5052952301- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- Acute Lymphoblastic Leukemia research
- MicroRNA in disease regulation
- Chronic Myeloid Leukemia Treatments
- Circular RNAs in diseases
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- Multiple Myeloma Research and Treatments
- Cancer-related gene regulation
- Viral-associated cancers and disorders
- Protein Degradation and Inhibitors
- Chronic Lymphocytic Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Renal and related cancers
- Radiopharmaceutical Chemistry and Applications
- Synthesis and Characterization of Heterocyclic Compounds
- RNA Interference and Gene Delivery
- Retinoids in leukemia and cellular processes
- Glycosylation and Glycoproteins Research
Huazhong University of Science and Technology
2014-2024
Qingdao University
2024
Affiliated Hospital of Qingdao University
2024
Tongji Hospital
2015-2024
Hubei Provincial Center for Disease Control and Prevention
2022
Huazhong University of Science and Technology Hospital
2013
Air Force Medical University
2000-2003
Xijing Hospital
2000
Acute myeloid leukemia (AML) is a highly heterogeneous hematological malignancy. Circular RNAs (circRNAs) play crucial roles in AML progression. This study aimed to explore the function and potential mechanism of circRNA Scm like with four mbt domains 2 (circ-SFMBT2; hsa_circ_0017639) AML.The levels circ-SFMBT2, microRNA-582-3p (miR-582-3p) zinc finger BTB domain containing 20 (ZBTB20) were measured by quantitative real-time PCR Western blot. Cell Counting Kit-8 (CCK-8), colony formation,...
Acute myeloid leukemia (AML) is a heterogeneous malignant disorder derived from the hematopoietic cells that accounts for ~80% of all adult acute leukemia. Numerous studies have shown drug resistance not only exists against conventional chemotherapeutic drugs, but also limits efficacy new biological agents. Therefore, it important to identify mechanisms behind chemoresistance and seek therapeutic strategies enhance in AML chemotherapy. MicroRNA (miR)-217 has been recognized as tumor...
Abstract Acute myeloid leukemia (AML) is a highly heterogeneous disease featured by clonal proliferation derived from primitive hematopoietic stem/progenitor cells. Circular RNAs (circRNAs) have been identified as crucial regulators in the progression of various cancers, including AML. However, molecular mechanism AML still not definite. This study aimed to explore influences circ_0012152 on cell development cells and underlying regulatory mechanism. The expression circ_0012152,...
Background Anaplastic lymphoma kinase-positive anaplastic large cell (ALK+ ALCL) is a rare, mature T-cell non-Hodgkin lymphoma. The prognosis of patients with relapsed or refractory ALCL following first-line chemotherapy extremely poor. NCCN guidelines recommend intensified without ASCT consolidation for r/r ALCL, however, this not an effective treatment all ALK+ALCL. Case report Herein, we patient relapsed/refractory ALK+ who received crizotinib and brentuximab vedotin as bridging therapy,...
This report investigates the sequence specificity requirements for homeodomain structure and DNA binding activity by design synthesis of a "minimAl" (for minimalist alanine scanning mutagenesis) which contains consensus residues in all nonconsensus have been replaced with alanine. The murine Msx served as prototype minimAl homeodomain, Ala-Msx. We show that Ala-Msx binds to specifically, albeit lower affinity than Msx. A derivative Ala-Msx(NT), native rather an alanine-substituted N-terminal...
Internal tandem duplication (ITD) mutations of FMS-like tyrosine kinase-3 (FLT3) are the most frequent genetic alterations in acute myeloid leukemia (AML) and predict a poor prognosis. FLT3 kinase inhibitors (TKIs) provide short-term clinical responses, but long-term prognosis FLT3/ITD+ AML patients remains poor. Notch signaling is important numerous types tumors. However, role to be elucidated. In current study, we found that was activated upon FLT3-TKI treatment cell lines primary cells....
Key Clinical Message Immune checkpoint inhibitors are a very popular method of treating malignant tumors. But its side effects cannot be ignored. This study revealed obstructive complications during immune consolidation therapy following sequential chimeric antigen receptor T cell with autologous hematopoietic stem transplantation in two patients diffuse large b lymphoma (DLBCL). Both our had the same symptoms vomiting and inability to eat due pyloric obstruction, it should highlighted that...
We have incorporated the DNA-cleaving moiety o-phenanthroline-copper at amino acid 10 of Msx-1 homeodomain, and we analyzed site-specific DNA cleavage by resulting derivative. show that homeodomain is close to 5' end consensus site 5'-(C/G)TAATTG-3' in Msx-1-DNA complex. Our results indicate orientation relative analogous engrailed Antennapedia homeodomains. further affinity cleaving permits identification sites for kilobase substrates. The specificity approach enabled us identify an within...
Abstract Novel analytic tools are needed to elucidate the molecular basis of leukemia-relevant gene mutations in post-genome era. We generated isogenic leukemia cell clones which FLT3 was disrupted a single allele using TALENs. Isogenic with mono-allelic were compared an wild-type control clone and parental cells for transcriptional expression, downstream signaling proliferation capacity. The global expression profiles mutant K562 corresponding controls RNA- seq . levels ligand-dependent...
PAX5 encodes a transcription factor essential for B-cell differentiation, and haploinsufficiency is involved in tumorigenesis. There were few studies on how regulated genes expression to promote In this study, we constructed the cell model of using gene editing technology Raji cells, detected differentially expressed used protein-protein interaction networks cluster analysis comprehensively investigate cellular pathways haploinsufficiency. The clusters transcription, inflammatory immune...
The 11q23 of the mixed lineage leukemia 1 ( MLL1 ) gene plays a crucial role in early embryonic development and hematopoiesis. MLL-AF9 fusion gene, resulting from chromosomal translocation, often leads to acute myeloid with poor prognosis. Here, we generated zebrafish model expressing human gene. Microinjection mRNA into embryos resulted enhanced hematopoiesis activation downstream genes such as meis1 hox cluster genes. Embryonic expression upregulated HSPC markers. Doxorubicin MI-2 (a menin...
Summary Glucocorticoids (GC) are used as the first‐line treatment of immune thrombocytopenia (ITP), but 10–20% patients insensitive to them. Regulatory T cells (Tregs) can maintain tolerance in autoimmune diseases. The present research pooled 55 with newly diagnosed ITP and 44 healthy volunteers from seven hospitals. All received GC were divided into GC‐sensitive GC‐insensitive groups according curative effect after 2 weeks treatment. levels lymphocyte subgroups Tregs recorded. As results...
Circular RNA (circRNA) regulates the pathogenesis of acute myeloid leukemia (AML). However, mechanism circRNA protein tyrosine kinase 2 (circPTK2) in AML remains unclear.Quantitative real-time polymerase chain reaction (qRT-PCR) assay was adopted for circPTK2, miR-582-3p and alpha-1,3-mannosyltransferase (ALG3) mRNA levels. 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay, 5'-ethynyl-2'-deoxyuridine (EdU) were conducted cell proliferation. Flow cytometry...