A5100624322- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Genetic diversity and population structure
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Random lasers and scattering media
- Market Dynamics and Volatility
- Neural Networks and Reservoir Computing
- Stock Market Forecasting Methods
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Hepatitis C virus research
- Orbital Angular Momentum in Optics
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Hepatitis B Virus Studies
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Financial Risk and Volatility Modeling
- Financial Markets and Investment Strategies
- Bacterial biofilms and quorum sensing
- Geological and Geochemical Analysis
Karolinska Institutet
2017-2025
City University of Hong Kong
2022-2025
City University of Hong Kong, Shenzhen Research Institute
2023-2025
Fujian University of Technology
2011-2024
Shandong Academy of Sciences
2023
Qilu University of Technology
2022-2023
University of Chinese Academy of Sciences
2023
Center for Excellence in Brain Science and Intelligence Technology
2023
Chinese Academy of Sciences
2023
Shanghai University
2021-2022
Songlin Chen and colleagues sequenced the whole genomes of a male (ZZ) female (ZW) Chinese half-smooth tongue sole, Cynoglossus semilaevis. Their analysis provides insights into structure evolution sex chromosomes adaptation to benthic lifestyle this flatfish. Genetic determination by W Z has developed independently in different groups organisms. To better understand plasticity sex-determination mechanisms, we sole (Cynoglossus semilaevis). In addition lifestyle, find that these fish are...
Sex-specific chromosomes, like the W of most female birds and Y male mammals, usually have lost genes owing to a lack recombination. We analyze newly available genomes 17 bird species representing avian phylogenetic range, find that more than half them do not as fully degenerated chromosomes chicken. show sex harbor tremendous diversity among in their composition pseudoautosomal regions degree Z/W differentiation. Punctuated events shared or lineage-specific recombination suppression...
Tigers and their close relatives (Panthera) are some of the world's most endangered species. Here we report de novo assembly an Amur tiger whole-genome sequence as well genomic sequences a white Bengal tiger, African lion, lion snow leopard. Through comparative genetic analyses these genomes, find signatures that may reflect molecular adaptations consistent with big cats' hypercarnivorous diet muscle strength. We leopard-specific determinant in EGLN1 (Met39>Lys39), which is likely to be...
Songlin Chen, Manfred Schartl, Qingyin Wang, Deborah M. Power and colleagues analyze the genome of Japanese flounder its transcriptome dynamics during metamorphosis. They report a role for thyroid hormone retinoic acid signaling, as well phototransduction pathways, in regulation craniofacial asymmetry. Flatfish have most extreme asymmetric body morphology vertebrates. During metamorphosis, one eye migrates to contralateral side skull, this migration is accompanied by extensive...
RNA-binding proteins (RBPs) regulate RNA metabolism at multiple levels by affecting splicing of nascent transcripts, folding, base modification, transport, localization, translation, and stability. Despite their central role in function, the specificities most RBPs remain unknown or incompletely defined. To address this, we have assembled a genome-scale collection domains (RBDs) assessed using high-throughput RNA-SELEX (HTR-SELEX). Approximately 70% for which obtained motif bound to short...
Abstract Background DNA methylation is an important epigenetic modification which has numerous roles in modulating genome function. Its levels are spatially correlated across the genome, typically high repressed regions but low transcription factor (TF) binding sites and active regulatory regions. However, mechanisms establishing genome-wide TF site patterns still unclear. Results Here we use a comparative approach to investigate association of evolution mammals. Specifically, experimentally...
Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the library, which act as unique molecular identifiers (UMIs) allow massively parallel lineage tracing and dropout screening. RSLs greatly improve reproducibility results increasing both precision accuracy screens. They reduce...
The gene desert upstream of the MYC oncogene on chromosome 8q24 contains susceptibility loci for several major forms human cancer. region shows high conservation between and mouse multiple enhancers that are activated in tumor cells. However, role this normal development has not been addressed. Here we show a 538 kb deletion entire super-enhancer mice results 50% to 80% decrease Myc expression tissues. viable no overt phenotype. they resistant tumorigenesis, most cells isolated from them...
Ducks have a typical avian karyotype that consists of macro- and microchromosomes, but pair much less differentiated ZW sex chromosomes compared to chickens. To elucidate the evolution chromosome architectures between ducks chickens, birds mammals, we produced nearly complete chromosomal assembly female Pekin duck by combining long-read sequencing multiplatform scaffolding techniques.
A high-throughput systematic evolution of ligands by exponential enrichment assay was applied to 371 putative TFs in Pseudomonas aeruginosa, which resulted the robust 199 unique sequence motifs describing binding specificities 182 TFs. By scanning genome, we predicted total 33,709 significant interactions between and their target loci, were more than 11-fold enriched intergenic regions but depleted gene body regions. To further explore delineate physiological pathogenic roles P. constructed...
We propose a ferrofluid transporter for digital droplet manipulation in an additive-free and lossless manner. The shows high reliability with on-chip design droplet-based bioanalysis throughput cell spheroid screening.
Sex chromosomes exhibit many unusual patterns in sequence and gene expression relative to autosomes. Birds have evolved a female heterogametic sex system (male ZZ, ZW), through stepwise suppression of recombination between chrZ chrW. To address the broad complex driving forces Z chromosome evolution, we analyze here 45 newly available bird genomes four species’ transcriptomes, over their course loss chromosomes. We show general significantly higher substitution rate introns synonymous...
Paralogous transcription factors (TFs) frequently recognize highly similar DNA motifs. Homodimerization can help distinguish them according to their different dimeric configurations. Here, by studying R2R3-MYB TFs, we show that homodimerization also directly change the recognized motifs between TFs. By high-throughput SELEX, profiled specificity landscape for 40 R2R3-MYBs of subfamily VIII and curated 833 motif models. The models homodimeric binding has evoked changes AtMYBs. Focusing on...
Cancer has been characterized as a wound that does not heal. Malignant cells are morphologically distinct from normal proliferating but have extensive similarities to tissues undergoing healing and/or regeneration. The mechanistic basis of this similarity has, however, remained enigmatic. Here, we show the genomic region upstream Myc , which carries more cancer susceptibility in humans than any other region, is required for intestinal regeneration after radiation damage. Failure regenerate...
The ostrich (Struthio camelus) is the tallest and heaviest living bird. Ostrich meat considered a healthy red meat, with an annual worldwide production ranging from 12,000 to 15,000 tons. As part of avian phylogenomics project, we sequenced genome for phylogenetic comparative genomics analyses. initial Illumina-based assembly this had scaffold N50 3.59 Mb total size 1.23 Gb. Since longer scaffolds are critical many genomic analyses, particularly chromosome-level analysis, generated optical...
The most essential issue facing the world today is provision of energy and sustainable consumption natural resources. Pretreatment an step to produce biofuels from lignocellulosic biomass. In this study, ammonia fiber explosion (AFEX) combined with NaOH (A-NaOH) pretreatment effects on characteristics Pennisetum sinese (herbaceous), oak (hardwood), camphor wood (softwood) were assessed using enzymatic efficiency analysis, thereby identifying composition properties subsequent bio-H2...
Abstract Sex-chromosome dosage represents a challenge for heterogametic species to maintain correct proportion of gene products across chromosomes in each sex. While therian mammals (XX/XY system) achieve near-perfect balance X-chromosome mRNAs through X-upregulation and X-inactivation, birds (ZW/ZZ have been found lack efficient compensation at RNA level, challenging the necessity resolving major gene-dosage discrepancies avian cells. Through allele-resolved multiome analyses, we...
This paper examines the price of carbon emission rights published by China Emissions Exchange (Shenzhen), analyzes statistical characteristics series and uses a generalized autoregressive conditional heteroskedasticity (GARCH) model to describe fluctuation risk formation mechanisms. The study shows following results: since 2013, China’s prices have become more stable. yield has gradually decreased market approached mature stage. However, after 2018, due factors such as economic downturn...