Camille Bellora
A5036410249- Molecular Biology Techniques and Applications
- Parkinson's Disease Mechanisms and Treatments
- Gut microbiota and health
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Blood donation and transfusion practices
- Toxin Mechanisms and Immunotoxins
- Biosimilars and Bioanalytical Methods
- Pluripotent Stem Cells Research
- Cancer Genomics and Diagnostics
- Diet and metabolism studies
- Autophagy in Disease and Therapy
- Statistical Methods in Clinical Trials
- Vitamin C and Antioxidants Research
- Cellular transport and secretion
- Mycobacterium research and diagnosis
- Nosocomial Infections in ICU
- Biomedical Ethics and Regulation
- Biochemical and Molecular Research
- Genetic factors in colorectal cancer
- Nuclear Receptors and Signaling
- Clostridium difficile and Clostridium perfringens research
- Folate and B Vitamins Research
- Carbohydrate Chemistry and Synthesis
- Reproductive System and Pregnancy
University of Luxembourg
2023-2024
Luxembourg Institute of Health
2021-2024
Central Bank of Luxembourg
2016-2022
National Library of Luxembourg
2015-2021
The gastrointestinal microbiome is a complex ecosystem with functions that shape human health. Studying the relationship between taxonomic alterations and functional repercussions linked to disease remains challenging. Here, we present an integrative approach resolve attributes of microbiota at metagenomic, metatranscriptomic metaproteomic levels. We apply our methods samples from four families multiple cases type 1 diabetes mellitus (T1DM). Analysis intra- inter-individual variation...
Abstract Parkinson’s disease (PD) is a highly heterogeneous disorder influenced by several environmental and genetic factors. Effective disease-modifying therapies robust early-stage biomarkers are still lacking, an improved understanding of the molecular changes in PD could help to reveal new diagnostic markers pharmaceutical targets. Here, we report results from cohort-wide blood plasma metabolic profiling patients controls Luxembourg Study detect disease-associated alterations at level...
Infections with SARS-CoV-2 have a pronounced impact on the gastrointestinal tract and its resident microbiome. Clear differences between severe cases of infection healthy individuals been reported, including loss commensal taxa. We aimed to understand if microbiome alterations functional shifts are unique or common effect COVID-19. used high-resolution systematic multi-omic analyses profile gut in asymptomatic-to-moderate COVID-19 compared control group.We found striking increase overall...
Metabolome analysis is complicated by the continuous dynamic changes of metabolites in vivo and ex vivo. One main challenges metabolomics robustness reproducibility results, partially driven pre-analytical variations.The objective this study was to analyse impact pre-centrifugation time temperature, determine a quality control marker plasma samples.Plasma were measured gas chromatography-mass spectrometry (GC-MS) analysed with MetaboliteDetector software. The metabolites, which most labile...
Abstract Heterozygous variants in the glucocerebrosidase GBA1 gene are an increasingly recognized risk factor for Parkinson’s disease (PD). Due to GBAP1 pseudogene, which shares 96% sequence homology with coding region, accurate variant calling by array-based or short-read sequencing methods remains a major challenge understanding genetic landscape of -associated PD. We analyzed 660 patients PD, 100 Parkinsonism and 808 healthy controls from Luxembourg study, sequenced using amplicon-based...
Abstract MicroRNAs act via targeted suppression of messenger RNA translation in the DNA–RNA–protein axis. The dysregulation microRNA(s) reflects epigenetic changes affecting cellular processes multiple disorders. To understand complex effect dysregulated microRNAs linked to neurodegeneration, we performed a cross-sectional microRNA expression analysis idiopathic Parkinson's disease (n = 367), progressive supranuclear palsy 35) and healthy controls 416) from Luxembourg Study, followed by...
Background: This is the third in a series of publications presenting formal method validation for biospecimen processing context accreditation laboratories and biobanks. We report here optimization stool protocol validated fitness-for-purpose terms downstream DNA-based analyses. Methods: Stool collection was initially optimized sample input quantity supernatant volume using canine stool. Three DNA extraction methods (PerkinElmer MSM I®, Norgen Biotek All-In-One®, MoBio PowerMag®) six...
Several phenotypic differences observed in Parkinson's disease (PD) patients have been linked to age at onset (AAO). We endeavoured find out whether these are due the ageing process itself by using a combined dataset of idiopathic PD (n = 430) and healthy controls (HC; n 556) excluding carriers known PD-linked genetic mutations both groups. found several significant effects AAO on motor non-motor symptoms PD, but when comparing with HC (using assessment, AAA), only positive associations AAA...
Next-generation sequencing (NGS) analyses on DNA derived from archived Formalin-Fixed Paraffin-Embedded (FFPE) clinical material can provide a powerful tool in oncology research and diagnostics. Although several studies have established that NGS be performed using FFPE tissue, the accuracy reproducibility of such analyses, as well their robustness to biomolecular quality samples used, remains matter debate. Excellent reviews recently been published, providing evidence-based best practices...
Abstract Background During the COVID-19 pandemic swift implementation of research cohorts was key. While many studies focused exclusively on infected individuals, population based are essential for follow-up SARS-CoV-2 impact public health. Here we present CON-VINCE cohort, estimate point and period prevalence infection, reflect spread within Luxembourgish population, examine immune responses to infection vaccination, ascertain psychological wellbeing at a nationwide level. Methods A...
Longer pre-centrifugation times alter the quality of serum and plasma samples. Markers for such delays in sample processing hence quality, have been identified.Twenty cytokines serum, EDTA citrate samples were screened changes concentration induced by extended blood at room temperature. The two that showed largest further validated their "diagnostic performance" identifying or with times.In this study, using R&D Systems ELISA kits, a delay longer than 24 h had an IL16 higher 313 pg/mL, IL8...
DNA extracted from formalin-fixed, paraffin-embedded tissue sections is often inadequate for sequencing, due to poor yield or degradation. We optimized the proteinase K digest by testing increased volume of enzyme and length manufacturer’s protocol using 54 biospecimens, performing in centrifuge tubes. Doubling quantity resulted a median increase 96%. Applying deparaffinized on microscope slides generated further 41%, but only at >50,000 epithelial tumor cells/section. now correlated with...
Since 2015, platelet products have been pathogen-inactivated (PI) at the Luxemburgish Red Cross (LRC) using Riboflavin and UV light (RF-PI). As LRC should respond to hospital needs any time, production exceeds demand, generating a discard rate of 18%. To reduce this, we consider extension storage time from 5 7 days. This study's objective was evaluate in vitro 7-day platelet-storage quality, comparing two PI technologies, RF-PI amotosalen/UVA (AM-PI), for pools whole-blood donations (PPCs)...
Abstract Background Efficient blood stabilization is essential to obtaining reliable and comparable RNA analysis data in preclinical operations. PAXgene (Qiagen, Becton Dickinson) Tempus (Applied Biosystems, Life Technologies) collection tubes with stabilizers both avoid preanalytical degradation of mRNA by endogenous nucleases modifications specific concentrations unintentional up- or down-regulation gene expression. Methods Sixteen different conditions were tested samples from seven...
Uncontrolled preanalytical variables can reduce the accuracy and reproducibility of downstream analytical results from peripheral blood mononuclear cells (PBMCs). PBMCs were isolated EDTA citrate-anticoagulated samples, obtained healthy subjects patients with inflammatory infectious conditions. PBMC-derived RNA samples examined for gene expression changes induced by extended pre-centrifugation delays at 4 °C RT. We used Taqman RTqPCR to evaluate combination two target genes their "diagnostic...
Background: Fibroblasts can be isolated from skin biopsies using a chemical dissociation, physical or combination of both techniques. They reprogrammed into induced pluripotent stem cells (iPSCs) through the introduction defined sets key transcription factors. This study aimed to identify optimal protocol for biopsy fibroblast culture, and cryopreservation in scope reprogramming iPSCs context biobank accreditation. Methods: First, four dissociation techniques typically used laboratory...
Abstract Background Parkinson's disease (PD), while often associated with its distinctive motor symptoms, can also exert a notable impact on the cardiovascular system due to development of severe autonomic dysfunction. One initial indicators PD is appearance dysautonomia. As such, it vital monitor and manage health individuals PD, as may have clinical implications in commonly recognized non-motor aspects disease. To study association history (CVD) occurrence severity here, we lend data CVD...
We evaluate the consequences of processing alcohol-fixed tissue in a processor previously used for formalin-fixed tissue. Biospecimens fixed PAXgene Tissue Fixative were cut into three pieces then processed flushed formalin-fixed, paraffin-embedded (FFPE) blocks (neutral buffered formalin [NBF] +ve ), formalin-free system (NBF −ve or left unprocessed. Histomorphology and immunohistochemistry compared using hematoxylin/eosin staining antibodies MLH-1, Ki-67, CK-7. Nucleic acid was extracted...
Development of novel biomarkers for diagnosis disease and assessment treatment efficacy utilizes a wide range biospecimens discovery research. The fitness the purpose biomarker development depends on clinical characteristics donor number critical potentially uncontrolled pre-analytical variables. Pre-analytical factors influence reliability to be analyzed can seriously impact analytic outcomes. Sample quality stratification assays tools utilized by biorepositories minimize bias resulting...