A5043795951- Alzheimer's disease research and treatments
- Protein Structure and Dynamics
- Research Data Management Practices
- Bioinformatics and Genomic Networks
- Scientific Computing and Data Management
- MicroRNA in disease regulation
- Genetic Associations and Epidemiology
- Amyotrophic Lateral Sclerosis Research
- Spectroscopy and Quantum Chemical Studies
- Parkinson's Disease Mechanisms and Treatments
- Biomedical Text Mining and Ontologies
- Enzyme Structure and Function
- Circular RNAs in diseases
- Dementia and Cognitive Impairment Research
- Cell Image Analysis Techniques
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Machine Learning in Bioinformatics
- Fuel Cells and Related Materials
- Microbial Metabolic Engineering and Bioproduction
- Chemical Synthesis and Analysis
- Computational Drug Discovery Methods
- Monoclonal and Polyclonal Antibodies Research
- Data Quality and Management
- Genetics, Bioinformatics, and Biomedical Research
Second Military Medical University
2017-2025
University of Luxembourg
2015-2024
Eurostat
2023-2024
Luxembourg Institute of Health
2023-2024
Dali University
2024
Yunnan Provincial Department of Education
2024
Shanghai Academy of Agricultural Sciences
2024
Molecular Biology Consortium
2023
Saarland University
2012-2022
Nanjing Normal University
2011-2022
Abstract Since 1992 PredictProtein (https://predictprotein.org) is a one-stop online resource for protein sequence analysis with its main site hosted at the Luxembourg Centre Systems Biomedicine (LCSB) and queried monthly by over 3,000 users in 2020. was first Internet server predictions. It pioneered combining evolutionary information machine learning. Given as input, outputs multiple alignments, predictions of structure 1D 2D (secondary structure, solvent accessibility, transmembrane...
Parkinson's disease (PD) is a systemic clinically defined by the degeneration of dopaminergic neurons in brain. While alterations gut microbiome composition have been reported PD, their functional consequences remain unclear. Herein, we addressed this question an analysis stool samples from Luxembourg Study (n = 147 typical PD cases, n 162 controls).All individuals underwent detailed clinical assessment, including neurological examinations and neuropsychological tests followed self-reporting...
Abstract The notion that data should be Findable, Accessible, Interoperable and Reusable, according to the FAIR Principles, has become a global norm for good stewardship prerequisite reproducibility. Nowadays, guides policy actions professional practices in public private sectors. Despite such endorsements, however, Principles are aspirational, remaining elusive at best, intimidating worst. To address lack of practical guidance, help with capability gaps, we developed Cookbook, an open,...
We have performed a detailed analysis of water clustering and percolation in hydrated Nafion configurations generated by classical molecular dynamics simulations. Our results show that at low hydration levels H2O molecules are isolated continuous hydrogen-bonded network forms as the level is increased. quantitative has established (λ) between 5 6 H2O/SO3− threshold Nafion. also examined effect such on proton transport studying structural diffusion protons using quantum hopping method. The...
Infections with SARS-CoV-2 have a pronounced impact on the gastrointestinal tract and its resident microbiome. Clear differences between severe cases of infection healthy individuals been reported, including loss commensal taxa. We aimed to understand if microbiome alterations functional shifts are unique or common effect COVID-19. used high-resolution systematic multi-omic analyses profile gut in asymptomatic-to-moderate COVID-19 compared control group.We found striking increase overall...
Abstract Parkinson’s disease (PD) is a highly heterogeneous disorder influenced by several environmental and genetic factors. Effective disease-modifying therapies robust early-stage biomarkers are still lacking, an improved understanding of the molecular changes in PD could help to reveal new diagnostic markers pharmaceutical targets. Here, we report results from cohort-wide blood plasma metabolic profiling patients controls Luxembourg Study detect disease-associated alterations at level...
The complete pathway of the association a proline-rich motif to an SH3 domain was probed by molecular dynamics simulations. results indicate bimodal binding mechanism, in which nature uses reduction dimensionality and hydrophobic dewetting as tools find simple solution seemingly complicated process.
The COVID-19 pandemic has highlighted the need for FAIR (Findable, Accessible, Interoperable, and Reusable) data more than any other scientific challenge to date. We developed a flexible, multi-level, domain-agnostic FAIRification framework, providing practical guidance improve FAIRness both existing future clinical molecular datasets. validated framework in collaboration with several major public-private partnership projects, demonstrating delivering improvements across all aspects of...
Common variants of about 20 genes contributing to AD risk have so far been identified through genome-wide association studies (GWAS). However, there is still a large proportion heritability that might be explained by rare but functionally important variants. One the with causing ADAM10. Using whole-genome sequencing we now single nonsynonymous variant (SNV) rs142946965 [p.R215I] in ADAM17 co-segregating an autosomal-dominant pattern late-onset one family. Subsequent genotyping and analysis...
Gastric cancer is one of the common malignant tumors worldwide. Increasing studies have indicated that circular RNAs (circRNAs) play critical roles in progression and shown great potential as useful markers therapeutic targets. However, precise mechanism functions most circRNAs are still unknown gastric cancer. In present study, we performed a microarray analysis to detect circRNA expression changes between tumor samples adjacent nontumor samples. The miRNA profiles were obtained from...
Translational medicine is a domain turning results of basic life science research into new tools and methods in clinical environment, for example, as diagnostics or therapies. Nowadays, the process translation supported by large amounts heterogeneous data ranging from medical to whole range -omics data. It not only great opportunity but also challenge, translational big difficult integrate analyze, requires involvement biomedical experts processing. We show here that visualization...
Abstract MicroRNAs act via targeted suppression of messenger RNA translation in the DNA–RNA–protein axis. The dysregulation microRNA(s) reflects epigenetic changes affecting cellular processes multiple disorders. To understand complex effect dysregulated microRNAs linked to neurodegeneration, we performed a cross-sectional microRNA expression analysis idiopathic Parkinson's disease (n = 367), progressive supranuclear palsy 35) and healthy controls 416) from Luxembourg Study, followed by...
This work focuses on the need for modeling and predicting adverse outcomes in immunotoxicology to improve nonclinical assessments of safety immunomodulatory therapies. The integrated approach includes, first, outcome pathway concept established toxicology field, and, second, systems medicine disease map describing molecular mechanisms involved a particular pathology. proposed workflow is illustrated with chimeric antigen receptor (CAR) T cell treatment as use case. To this end, linear (AOP)...
The binding competition between a proline-rich motif (PRM) ligand and hydrophobic nanoparticle, the single-wall carbon nanotube (SWCNT), at pocket of SH3 domain, has been investigated by molecular dynamics simulations. It is found that SWCNT very high probability occupying which prevents PRM from to pocket. free energy landscapes show ∼0.6 kcal/mol stronger affinity than in three-way (SWCNT + protein). potent domain shown be mainly π–π stacking interactions CNT aromatic residues Our findings...
Abstract Frontotemporal dementia (FTD) is a neurodegenerative disorder predominantly affecting the frontal and temporal lobes. Genome-wide association studies (GWAS) on FTD identified only few risk loci. One of possible explanations that clinically, pathologically, genetically heterogeneous. An important open question to what extent epigenetic factors contribute whether these vary between clinical subgroup. We compared DNA-methylation levels cases (n = 128), with Amyotrophic Lateral...
Introduction Late-onset Alzheimer’s disease (LOAD, onset age > 60 years) is the most prevalent dementia in elderly 1 , and risk partially driven by genetics 2 . Many of loci responsible for this genetic were identified genome-wide association studies (GWAS) 3–8 To identify additional LOAD loci, we performed largest GWAS to date (89,769 individuals), analyzing both common rare variants. We confirm 20 previous four new ( IQCK ACE ADAM10 ADAMTS1 ). Pathway analysis these data implicates...
Abstract Heterozygous variants in the glucocerebrosidase GBA1 gene are an increasingly recognized risk factor for Parkinson’s disease (PD). Due to GBAP1 pseudogene, which shares 96% sequence homology with coding region, accurate variant calling by array-based or short-read sequencing methods remains a major challenge understanding genetic landscape of -associated PD. We analyzed 660 patients PD, 100 Parkinsonism and 808 healthy controls from Luxembourg study, sequenced using amplicon-based...
Cyclophilin A (CypA) is a peptidyl-prolyl cis/trans-isomerase that involved in multiple signaling events of eukaryotic cells. It might either act as catalyst for prolyl bond isomerization, or it can form stoichiometric complexes with target proteins. We have investigated the linear sequence recognition code CypA by phage display and found consensus motif FGPXLp to be selected after five rounds panning. The peptide FGPDLPAGD showed inhibition isomerase reaction NMR chemical shift mapping...