A5001230865- Wound Healing and Treatments
- Genomics and Phylogenetic Studies
- Skin Protection and Aging
- Gut microbiota and health
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- Microbial Community Ecology and Physiology
- Hair Growth and Disorders
- Advanced Glycation End Products research
- Environmental DNA in Biodiversity Studies
- Cancer-related molecular mechanisms research
- melanin and skin pigmentation
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- Facial Nerve Paralysis Treatment and Research
- Liver Disease Diagnosis and Treatment
- Diabetes, Cardiovascular Risks, and Lipoproteins
- CRISPR and Genetic Engineering
- Electrospun Nanofibers in Biomedical Applications
- Insect symbiosis and bacterial influences
- Facial Rejuvenation and Surgery Techniques
- Parasitic Diseases Research and Treatment
- Dermatologic Treatments and Research
- Diabetes Management and Research
- Parasite Biology and Host Interactions
Xuzhou Central Hospital
2023
Zhejiang University of Technology
2019-2023
ShenZhen People’s Hospital
2022
Jinan University
2019-2022
Southern University of Science and Technology
2019-2022
Shandong First Medical University
2022
Shandong Tumor Hospital
2022
BGI Group (China)
2012-2021
Harbin Medical University
2018-2021
MGI
2019
Abstract Adipose-derived stem cells (ADSCs) show potential in skin regeneration research. A previous study reported the failure of full-thickness self-repair an injury area exceeding 4 cm diameter. Stem cell therapies have shown promise accelerating regeneration; however, low survival rate transplanted due to lack protection during and after transplantation leads efficacy. Hence, effective biomaterials for delivery retention ADSCs are urgently needed purposes. Here, we covalently crosslinked...
To develop a precise personalized type 2 diabetes mellitus (T2DM) prediction model by cost-effective and readily available parameters in Central China population. A 3-year cohort study was performed on 5557 nondiabetic individuals who underwent annual physical examination as the training cohort, subsequent validation of 1870 conducted using same procedures. Multiple logistic regression analysis performed, simple nomogram constructed via stepwise method. Receiver operating characteristic...
Metabarcoding is a widely used method for fast characterization of microbial communities in complex environmental samples. However, the selction sequencing platform can have noticeable effect on estimated community composition. Here, we evaluated metabarcoding performance DNBSEQ-G400 sequencer developed by MGI Tech using 16S and internal transcribed spacer (ITS) markers to investigate bacterial fungal mock communities, as well ITS2 marker 1144 soil samples, with additional technical...
To study the changes in hair follicle cycle and related stem cells induced by photoaging to establish a mouse model of senescence follicles.There were 54 C57BL6/J mice randomly divided into three groups. The UVA group UVB underwent UV lamps for 8 weeks. Changes skin compared physical signs, dermoscopy, hematoxylin eosin Masson's staining each group. Western blot, immunohistochemistry, RT-qPCR carried out test canonical proteins gene expression Wnt signaling pathway samples....
Skin is a dynamic organ that maintains homeostasis and provides protection against environmental stimuli pathogens. However, constant solar ultraviolet (UV) radiation can induce photoaging photocarcinogenesis, thus reducing skin barrier function by altering at the cellular structural levels. Adipose-derived stem cells (ADSCs) ameliorate signs of photoaging, but their antiphotoaging mechanism remains elusive. In this study, we explored which ADSCs improve photoaging.Female C57BL/6J mice were...
Background/Aims: Skin photoaging is primarily caused by the functional attrition of skin stem cells. The cell niche plays an important role in maintaining survival and behaviour. In our study, we hypothesized that UVB irradiation induces changing niches transferred adipose-derived cells (ADSCs) can remodel affecting BMP signalling pathway transdifferentiating into Methods: Sixty-four C57BL/6J mice were divided following groups: a control group, group UVB+ADSCs group. Western blot assays,...
Abstract Massively parallel sequencing (MPS) on DNA nanoarrays provides billions of reads at relatively low cost and enables a multitude genomic applications. Further improvement in read length, sequence quality reduction will enable more affordable accurate comprehensive health monitoring tests. Currently the most efficient MPS uses dye-labeled reversibly terminated nucleotides (RTs) that are expensive to make challenging incorporate. Furthermore, part dye-linker (scar) remains nucleobase...
BACKGROUND Important differences in facial anatomy and how faces age must be considered when performing facelifts Asian populations. Few facelift methods are specifically designed for patients. OBJECTIVE This study evaluated the efficacy of lateral superficial muscular aponeurotic system (SMAS)-stacking/SMAS-ectomy with orbicularis–malar fat repositioning. MATERIALS AND METHODS Between February 2013 December 2016, 62 women underwent technique completed follow-up (15 months, ranging from 3 to...
Abstract Background Systematic errors can be introduced from DNA amplification during massively parallel sequencing (MPS) library preparation and array formation. Polymerase chain reaction (PCR)-free genomic methods were previously shown to improve whole genome (WGS) quality on the Illumina platform, especially in calling insertions deletions (InDels). We hypothesized that substantial InDel continue by remaining PCR step of cluster generation. In addition sequencing, data analysis are also...
ABSTRACT Metabarcoding has become the de facto method for characterizing structure of microbial communities in complex environmental samples. To determine how sequencing platform may influence community characterization, we present a large-scale comparison two platforms; Illumina MiSeq and new DNBSEQ-G400 developed by MGI Tech. The accuracy on bacterial fungal mock samples compared consistency precision between platforms ITS2 region from 1144 soil with 3 technical replicates. showed high...
Abstract Background :Pressure ulcers (PUs) are a type of chronic wound in the elderly population. Previous studies have shown that exosomes derived from stem cells contain cytokines and growth factors affect tissue repair can represent therapeutic strategy for healing. Thus, fully understanding how to extract their mechanism action help promote management refractory healing as new cell-free treatment model. Methods :In this study, we isolated human umbilical cord mesenchymal (hucMSC-Exos)...
Objective: To explore the ideal way of using stereoscopic unequal S-plasty in reconstruction congenital hypoplastic ear lobe cleft with soft tissue deficiency. Methods: Data 10 patients associated deficiency who were treated plastic cosmetic surgery Xuzhou Central Hospital from Aug 2018 to 2022 retrospectively analyzed. Six male, 4 female. Their ages ranged 6 years 19 old, a mean age 13 years. Lobe size 0.8 cm×0.5 cm 1.2 cm×1.0 cm. Results: The post-operation flaps had no venous congestion,...
Abstract Background: Pressure ulcers (PU) are a chronic wound for elderly populations. Previous works have shown that exosomes from stem cells contain cytokines and growth factors play role in tissue repair can represent therapeutic strategy healing. Thus, as new cell-free treatment model, fully understanding the extraction of its mechanism action help promote management clinically refractory Material Methods: In this study, we initially isolated human umbilical cord mesenchymal (hucMSC-Exo)...