A5078163920- Antibiotic Resistance in Bacteria
- Adipose Tissue and Metabolism
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Adipokines, Inflammation, and Metabolic Diseases
- Clinical practice guidelines implementation
- Genomics and Phylogenetic Studies
- Lipid metabolism and biosynthesis
- Antimicrobial Peptides and Activities
- Healthcare Systems and Technology
- Pancreatic function and diabetes
- Vibrio bacteria research studies
- Nail Diseases and Treatments
- Medical and Biological Sciences
- Medicine and Dermatology Studies History
- Endoplasmic Reticulum Stress and Disease
- Dermatological and COVID-19 studies
- Antimicrobial Resistance in Staphylococcus
- Pharmaceutical and Antibiotic Environmental Impacts
- Cannabis and Cannabinoid Research
- Cutaneous Melanoma Detection and Management
- Regulation of Appetite and Obesity
- Bacterial Infections and Vaccines
Universidad de Sevilla
2013-2024
Instituto de Biomedicina de Sevilla
2013-2024
Hospital Universitario Virgen Macarena
2022-2024
Centro de Investigación Biomédica en Red
2008-2024
Instituto de Salud Carlos III
2010-2024
Indra (Spain)
2022
John Wiley & Sons (United States)
2022
American Dental Education Association
2022
Washington University in St. Louis
2017
Midwestern University
2014
GPR55 is a putative cannabinoid receptor, and l-α-lysophosphatidylinositol (LPI) its only known endogenous ligand. We investigated 1) whether expressed in fat liver; 2) the correlation of both LPI with several metabolic parameters; 3) actions on human adipocytes. analyzed CB1, CB2, gene expression circulating levels two independent cohorts obese lean subjects, normal or impaired glucose tolerance type 2 diabetes. Ex vivo experiments were used to measure intracellular calcium lipid...
Lipid droplets (LDs) are organelles that coordinate lipid storage and mobilization, both processes being especially important in cells specialized managing fat, the adipocytes. Proteomic analyses of LDs have consistently identified small GTPase Rab18 as a component LD coat. However, specific contribution to adipocyte function remains be elucidated. Herein, we analyzed expression, intracellular localization relation metabolic status We show production increases during adipogenic...
Pathogenic Acinetobacter species, including baumannii and nosocomialis, are opportunistic human pathogens of increasing relevance worldwide. Although their mechanisms drug resistance well studied, the virulence factors that govern pathogenesis incompletely characterized. Here we define complete secretome A. nosocomialis strain M2 in minimal medium demonstrate pathogenic species produce both a functional type I secretion system (T1SS) contact-dependent inhibition (CDI) system. Using...
Acinetobacter baumannii can acquire resistance to colistin via complete loss of lipopolysaccharide (LPS) biosynthesis due mutations in the lpxA, lpxC and lpxD genes. However, although is increasingly being used for treatment multidrug resistant infections, very few A. clinical isolates develop through LPS biosynthesis. This may suggest that affects virulence traits play a role transmission pathogenesis baumannii. In this study we characterize multiple phenotypes resistant, LPS-deficient...
Abstract Adipose tissue represents a complex both in terms of its cellular composition, as it includes mature adipocytes and the various cell types comprising stromal‐vascular fraction (SVF), relation to distinct biochemical, morphological functional characteristics according anatomical location. Herein, we have characterized proteomic profile adipocyte SVF from human visceral adipose (VAT) subcutaneous (SAT) fat depots order unveil differences expression proteins which may underlie...
Lipodystrophy is a major disease involving severe alterations of adipose tissue distribution and metabolism. Mutations in genes encoding the nuclear envelope protein lamin A or its processing enzyme, metalloproteinase Zmpste24, cause diverse human progeroid syndromes that are commonly characterized by selective loss tissue. Similarly to humans, mice deficient Zmpste24 accumulate prelamin display phenotypic features accelerated aging, including lipodystrophy. Herein, we report proteome...
The increasing clinical importance of infections caused by multidrug resistant Acinetobacter baumannii warrants the development novel approaches for prevention and treatment. In this context, vaccination certain patient populations may contribute to reducing morbidity mortality pathogen. Vaccines against Gram-negative bacteria based on inactivated bacterial cells are highly immunogenic have been shown produce protective immunity a number species. However, high endotoxin levels present in...
LpxC inhibitors have generally shown poor in vitro activity against Acinetobacter baumannii We show that the inhibitor PF-5081090 inhibits lipid A biosynthesis, as determined by silver staining and measurements of endotoxin levels, significantly increases cell permeability. The presence at 32 mg/liter increased susceptibility to rifampin, vancomycin, azithromycin, imipenem, amikacin but had no effect on ciprofloxacin tigecycline. Potentiating existing antibiotics with may represent an...
Aims: Cannabinoids are known to control energy homeostasis. Atypical cannabinoids produce pharmacological effects via unidentified targets. We sought investigate whether the atypical cannabinoid O‐1602 controls food intake and body weight. Methods: The rats were injected acutely or subchronically with O‐1602, expression of several factors involved in adipocyte metabolism was assessed by real‐time polymerase chain reaction. In vivo findings corroborated vitro studies incubating 3T3‐L1...
ABSTRACT Acinetobacter baumannii can acquire resistance to the cationic peptide antibiotic colistin through complete loss of lipopolysaccharide (LPS) expression. The activities host antimicrobials LL-37 and human lysozyme against multidrug-resistant clinical isolates A. that acquired were characterized. We demonstrate has activity strains lacking is similar their colistin-sensitive parent strains, whereas increased colistin-resistant LPS.
Abstract The adipokine resistin is an insulin-antagonizing factor that also plays a regulatory role in inflammation, immunity, food intake, and gonadal function. Although adipose tissue the primary source of resistin, it expressed other tissues organs, including pituitary. However, there no information on whether as described previously for adipokines such leptin adiponectin, could regulate this gland. Likewise, molecular basis actions remains largely unexplored. Here we show administration...
In recent years, new evidence has shown that the SOS response plays an important role in to antimicrobials, with involvement generation of clinical resistance. Here we evaluate impact heterogeneous expression isolates Escherichia coli on fluoroquinolone, ciprofloxacin. silico analysis whole genome sequencing data showed remarkable sequence conservation regulators, RecA and LexA. Despite genetic homogeneity, our results revealed a marked differential heterogeneity activation, both at...
Rab proteins regulate the sequential steps of intracellular membrane transport. Alterations these GTPases and their associated are emerging as underlying cause for several human diseases involving dysregulated secretory activities.Herein we investigated role Rab18, which negatively regulates hormone secretion by interacting with granules, in relation to altered functioning tumoral pituitary somatotropes causing acromegaly.A total 18 patients diagnosed tumors acromegaly (nine patients) or...