A5100629099- Heme Oxygenase-1 and Carbon Monoxide
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Dental materials and restorations
- Adrenal Hormones and Disorders
- Bone Tissue Engineering Materials
- Parvovirus B19 Infection Studies
- Peptidase Inhibition and Analysis
- Animal Virus Infections Studies
- Cancer Cells and Metastasis
- Protease and Inhibitor Mechanisms
- Influenza Virus Research Studies
- Pituitary Gland Disorders and Treatments
- Neonatal Health and Biochemistry
- Fibroblast Growth Factor Research
- Inflammation biomarkers and pathways
- Silk-based biomaterials and applications
- Media Influence and Health
- Eicosanoids and Hypertension Pharmacology
- Genetics and Neurodevelopmental Disorders
Affiliated Hospital of Southwest Medical University
2025
Tianjin Medical University General Hospital
2020-2024
Peking University
2005-2023
Shandong Provincial Hospital
2017-2022
Shandong First Medical University
2022
Mellanox Technologies (Israel)
2021
Dalian Medical University
2021
Shenzhen Bioeasy Biotechnology (China)
2021
Shandong University
2017
First Affiliated Hospital of Zhengzhou University
2017
The proto-oncogene c-Myc regulates multiple biological processes mainly through selectively activating gene expression. However, the mechanisms underlying c-Myc-mediated repression in context of cancer remain less clear. This study aimed to clarify role PRMT5 transcriptional target genes gastric cancer. Methods: Immunohistochemistry was used evaluate expression PRMT5, and patients. interaction assessed by immunofluorescence, co-immunoprecipitation GST pull-down assays. Bioinformatics...
The tumour microenvironment is a highly heterogeneous entity that plays crucial roles in cancer progression. As the most prominent stromal cell types, cancer-associated fibroblasts (CAFs) produce variety of factors into microenvironment. In present study, we firstly isolated CAFs from tissues patients with ovarian and demonstrated hepatocyte growth factor (HGF) was expressed supernatants CAFs. CAF-derived HGF or human recombinant promoted proliferation lines SKOV3 HO-8910 cells. Western...
This study is to explore the therapeutic effect and potential mechanisms of exosomal microRNAs (miRNAs) derived from ovaries with primary ovarian insufficiency (POI). The POI mouse model was established by intraperitoneal injection cyclophosphamide (CTX) busulfan. apoptosis granulosa cells (GCs) incubated exosomes extracted tissues control groups analyzed flow cytometry. Then, high-throughput sequencing performed detect difference miRNAs profile in tissue-derived between mice. differential...
Bioengineering functional hepatic tissue constructs that physiologically replicate the human native liver in vitro is sought for clinical research and drug discovery. However, intricate architecture specific biofunctionality possessed by remain challenging to mimic vitro. In present study, a versatile strategy fabricate lobular-like silk protein scaffolds with radially aligned lamellar sheets, interconnected channels, converging central cavity was designed implemented. A proof-of-concept...
The present study was performed to investigate the effects of 4-chloro-DL-phenylalanine (PCPA), a tryptophan hydroxylase (Tph) inhibitor (TphI), on pulmonary vascular remodeling and lung inflammation in monocrotaline (MCT)-induced arterial hypertension (PAH) rats. Animal models PAH were established using Sprague-Dawley (SD) rats by single intraperitoneal injection MCT (60 mg/kg). PCPA (50 or 100 mg/kg/day) administered with PAH. On day 22, hemodynamic measurements morphological observations...
Abstract Background The VP3 protein of goose parvovirus (GPV) or Muscovy duck (MDPV), a major structural protein, can induce neutralizing antibodies in geese and ducks, but monoclonal (MAbs) against has never been characterized. Results Three hybridoma cell lines secreting anti-GPV MAbs were obtained designated 4A8, 4E2, 2D5. Immunoglobulin subclass tests differentiated them as IgG2b (4A8 4E2) IgG2a (2D5). Dot blotting assays showed that three reacted with His-VP3 conformation-independent...
The present study aimed to investigate the molecular basis of lung cancer development using a microarray identify differentially-expressed genes associated with various tumor-node-metastasis (TNM) stages adenocarcinoma. This subtype has increased in incidence within recent years China. A 35K oligo gene array covering ~25,100 was used screen among 90 adenocarcinoma samples TNM stages. To verify data from arrays, three [human zinc finger-containing, Miz1, PIAS-like protein on chromosome 7...
Differentiating between adrenal Cushing syndrome (adrenal CS) and disease (CD) can be challenging if there are equivocal or falsely elevated adrenocorticotropic hormone (ACTH) values. We aim to investigate the diagnostic value of serum steroid profiles in differentiating CS from CD.A total 11 steroids (n = 13) CD 15) were analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Age- gender-specific ratios generated dividing actual concentration upper limit relevant...
Transcriptional repression is as important transcriptional activation in establishing cell-type specific patterns of gene expression. RE1-silencing transcription factor (REST), also known neuronal restrictive silencing (NRSF), a regulator that represses battery differentiation genes non-neuronal cells or neural progenitor by binding to DNA sequence (repressor element-1/neuron-restrictive silencer element, RE1/NRSE). REST/NRSF functions the development are widely studied, however, little...
This study investigated the association between polymorphisms (−607A/C and −137G/C) in promoter region of IL18 gene (which encodes interleukin [IL]-18) serum levels IL-18, using standard genotyping techniques (sequence specific primer—polymerase chain reaction) an enzyme-linked immunosorbent assay, respectively, patients allergic to penicillin. A higher frequency alleles AA genotype was found at position −607A/C penicillin than control subjects. For −137G/C position, C allele more frequent...
Thymidylate synthase (TS), which catalyzes the de novo synthesis of dUMP, is an important target for cancer therapy. In this report, effects 5-fluorouracil (5-FU) and ZD1694 on regulation TS gene expression were evaluated in zebrafish embryos. Our results revealed that was increased by about six-fold when embryos treated with 1.0 µM 5-FU there a greater than 10-fold increase protein level after treatment 0.4 ZD1694. Northern blot analysis confirmed mRNA identical or untreated Gel shift...
The purpose of this study was to explore the inhibiting role MMP-9 gene silence in invasive ability and growth laryngeal squamous cell carcinoma (LSCC) by lentivirus mediated RNA interference. MMP-9-RNAi-lentivirus control (GFP-lentivirus) were transfected into Hep-2 cells. Gelatin zymography showed proteins expression knockdown siRNA activity viability treated cells decreased than measured with modified Boyden chamber assay MTT assay. In animal experiment, 20 nude mice bearing tumor...
The epithelium is a highly dynamic system, which plays crucial role in the homeostasis of intestinal tract. However, studies on physiological and pathophysiological functions epithelial cells (IECs) have been hampered due to lack normal cell models. In present study, we established reproducible method for primary culture mouse IECs, were isolated from viable small crypts murine fetuses (on embryonic day 19), using type I collagenase hyaluronidase short span time (≤20 min). With this method,...
Accumulating evidence has revealed that the mammalian heart possesses a measurable capacity for renewal. Neonatal mice retain regenerative over short time-frame (≤6 days), but this is lost by 7 days of age. In present study, differential gene expression profiling mouse cardiac tissue was performed to further elucidate mechanisms underlying process. The global patterns neonatal C57BL/6J were examined at three key time-points (1, 6 and old) using digital analysis. distribution total clean...
ZnO thin films with strong c-axis preferred orientation have been deposited on Ti buffered Si （111） substrate by reactive ratio-frequency magnetron sputtering. With X-ray diffraction analysis and photoluminescence （PL） measurement， the structure， residual stress PL emission properties of Ti-buffered are studied effects Ti-layer thickness annealing temperature discussed. It is found that improved after introduction buffer layer an optimized given. Annealing treatment can improve...
Teachers are transmitter of knowledge, skills and values to the mass population, therefore they considered as nation's valuable asset. Regardless teaching experiences, teachers required play their roles utmost capabilities. However, research clearly showed that, experience shaped teachers' perceptual, thinking behavior A set questionnaire was developed aimed identify personal constructs between novice expert Science teacher concerning function. Two groups with different level in formed. One...