A5101793089- Organ Transplantation Techniques and Outcomes
- Pancreatic function and diabetes
- Liver Disease and Transplantation
- Transplantation: Methods and Outcomes
- Organ Donation and Transplantation
- Renal Transplantation Outcomes and Treatments
- Liver Disease Diagnosis and Treatment
- Diabetes and associated disorders
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Immunotherapy and Immune Responses
- Diet, Metabolism, and Disease
- MicroRNA in disease regulation
- RNA modifications and cancer
- Diabetes Management and Research
- Pancreatic and Hepatic Oncology Research
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Ferroptosis and cancer prognosis
- Cardiac Ischemia and Reperfusion
- Cannabis and Cannabinoid Research
- Mitochondrial Function and Pathology
- Cancer Mechanisms and Therapy
First Hospital of China Medical University
2011-2025
China Medical University
2011-2025
Northwest University
2025
Jilin Province Tumor Hospital
2019
Quzhou City People's Hospital
2018
Chengdu Military General Hospital
2007
Abstract Background Emerging evidence has shown that dysregulated expression of long noncoding RNAs (lncRNAs) is implicated in liver hepatocellular carcinoma (HCC). However, the role and molecular mechanism differentially expressed lncRNAs HCC not been fully explained. Methods The profiles samples were derived from microarrays analysis or downloaded Cancer Genome Atlas (TCGA), their correlation with prognosis clinical characteristics further analyzed. Silencing lncRNA ZFPM2-AS1 was conducted...
Increasing evidence suggests that microRNAs (miRNAs) play critical roles in malignant transformation, tumor progression and metastasis. Aberrant miR-655-3p expression has been associated with several cancers. However, the role underlying mechanism of development hepatocellular carcinoma (HCC) remains unclear. MiR-655-3p was detected by quantitative RT-PCR (qRT-PCR) human HCC tissues cell lines. Cell proliferation investigated using MTT colony formation assays, migration invasion abilities...
Background We investigated whether the outcome of organs from donors after circulatory death (DCD) can be improved by addition mcc950 to perfusate hypothermic machine perfusion (HMP) system and intravenous injection transplantation in a pig liver model. Methods Thirty-six healthy Bama mini pigs randomized into 3 groups. All DCD livers were preserved an HMP 2 hours simple cold storage. In HMP-Postop group, was added perfusate; control group Postop normal LPS. After transplantation,...
ABSTRACT Organ transplantation is the most effective treatment for end‐stage organ disease. One of major challenges in shortage. For this reason, more and extended criteria donor organs, including those from donation after circulatory determination death (DCDD), are used clinical practice. However, DCDD organs suffer additional warm ischemic damage, which seriously affects transplant outcomes utilization. Recent studies at home abroad have shown that application normothermic regional...
Pancreatic islet transplantation is a promising treatment for type 1 diabetes (T1D). Interleukin-35 (IL-35) recently discovered cytokine that exhibits potent immunosuppressive functions. However, the role of IL-35 in transplant rejection remains to be elucidated. In this study, we isolated cells BALB/c mouse and purified CD4+ T cell subsets C57BL/6 mouse. The model was established vitro by co-culture cells. (20 ng/ml) administered every other day. Following co-culture, function Treg/Th17...
Purpose. Treatment outcomes for advanced liver cancer are poor. Immunotherapy is a treatment strategy that has been widely used to treat other cancers. Studies have shown CD8+ T lymphocytes essential factors affecting the efficacy of immunotherapy. We computational biology methods determine coexpressed gene network promotes lymphocyte infiltration. Method. obtained matrix and clinical follow-up information data from TCGA hepatocellular carcinoma FPKM. single nucleotide polymorphism (SNP)...
Background: Ursolic acid (UA) possesses extensive pharmacological activities, including anti-oxidation, anti-infection, anti-inflammation, anti-tumor, liver protection. This study was designed to investigate the effect of UA on transplantation after using donation cardiac death (DCD), and assess mechanisms. Methods: 24 healthy experimental pigs were randomly divided into control groups. Each group received six DCD transplantations. In group, recipient 120 mg/kg 4 h before surgery by...
For years, procalcitonin (PCT) has been employed as a diagnostic biomarker for the severity of sepsis and septic shock, well guiding application antibiotics. However, molecular/cellular basis regulation PCT production is not fully understood. In this study, we identified signalling pathway by which expression was induced lipopolysaccharide in human hepatocytes at mRNA protein levels. This dependent on nuclear transcription factor κB (NF-κB), indicated NF-κB binding site (nt -53 to -44) found...
To explore the association between expression of Ras and Rab interactor 1 (RIN1) prognosis hepatocellular carcinoma (HCC).RIN1 was detected in paired HCC tissues by real-time polymerase chain reaction, Western blot analysis, immunohistochemistry. Transfection applied to analyze RIN1 function.We found that protein downregulated samples compared with corresponding normal tissues. Downregulation also associated invasion poor overall survival (OS). The results our multivariate analysis indicated...
In this study, a dynamic three-dimensional cell culture technology was used to expand and differentiate rat pancreatic ductderived stem cells (PDSCs) into islet-like clusters that can secrete insulin.PDSCs were isolated from tissues by in situ collagenase digestion density gradient centrifugation.Using technique, the expanded differentiated functional clusters, which characterized morphological phenotype analyses.After maintaining 1 x 10 8 PDSCs for 7 days, 1.5 9 could be harvested.Passaged...
Rapid, efficient, and economic method for the isolation purification of islets has been pursued by numerous islet-related researchers. In this study, we compared advantages disadvantages our developed patented with those commonly used conventional methods (Ficoll-400, 1077, handpicking methods). Cell viability was assayed using Trypan blue, cell purity yield were diphenylthiocarbazone, islet function acridine orange/ethidium bromide staining enzyme-linked immunosorbent assay-glucose...
To investigate effect of warm ischemia after cardiac death on activation TLR9 pathway in porcine liver.Donor (DCD) model was established with Duroc, Landrace, Large White crossbred pigs. Liver tissues from the animals were harvested at 0, 5, 10, 15, 25 and 30 minutes for analysis expression TLR9, IRF7, IFN-β, TNF-α mRNA protein levels by real-time PCR western blot, respectively, assessment NF-κB/DNA binding activity blot detection p65 protein.Ischemia induced both an ischemic time dependent...
Objective This study aimed to analyze the population characteristics of apheresis platelet donors in Chongqing Province and provide a scientific basis for development precise efficient recruitment strategies. The ultimate goal is increase number regular preparation public health emergencies. Methods involved 53,089 blood who donated platelets Blood Center from 2020 2022. Data regarding age, sex, type, education level, occupation, frequency donation were collected analyzed identify factors...
Hepatocellular carcinoma (HCC) ranks among the most prevalent and lethal malignancies worldwide. Histone modifications (HMs) play a pivotal role in initiation progression of HCC. However, our understanding HMs HCC remains limited due to disease's heterogeneity complexity HMs.
AIM: CMU-1 is a new preservation solution with low potassium concentration as well viscosity that highly effective in reducing injury.The purpose of this experiment to compare the protective effect UW during cold and normothermic reperfusion. METHODS:Wistar rats were divided into two groups according different solution: group group.After 6, 12 24 h storage rat liver solutions, isolated perfused model was applied reperfuse for 120 min normothermically (37 ℃) Krebs-Henseleit solution,...