A5100784014- Ion Channels and Receptors
- Ion Transport and Channel Regulation
- Aldose Reductase and Taurine
- Ion channel regulation and function
- Multiple Myeloma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Viral gastroenteritis research and epidemiology
- Child and Adolescent Psychosocial and Emotional Development
- Monoclonal and Polyclonal Antibodies Research
- Biochemical Analysis and Sensing Techniques
- Health disparities and outcomes
- Skin and Cellular Biology Research
- Prenatal Substance Exposure Effects
- Cardiac Health and Mental Health
- Maternal Mental Health During Pregnancy and Postpartum
- Management of metastatic bone disease
- Metabolomics and Mass Spectrometry Studies
- Water Quality and Pollution Assessment
- Obesity, Physical Activity, Diet
- Circular RNAs in diseases
- Bioinformatics and Genomic Networks
- Cancer therapeutics and mechanisms
- TGF-β signaling in diseases
- Iron Metabolism and Disorders
- RNA Interference and Gene Delivery
Harbin Medical University
2011-2025
The First People's Hospital of Changde
2025
Central South University
2025
Zunyi Medical University
2024-2025
Shanxi Medical University
2023-2024
Nanjing Medical University
2021-2024
Jiangsu Province Hospital
2021-2024
Eli Lilly (United States)
2024
Jiamusi University
2023
China Three Gorges University
2012-2022
The recently identified transient receptor potential (TRP) channel family member, TRPV4 (formerly known as OTRPC4, VR-OAC, TRP12, and VRL-2) is activated by hypotonicity. It highly expressed in the kidney well blood-brain barrier-deficient hypothalamic nuclei responsible for systemic osmosensing. Apart from its gating hypotonicity, little about regulation. We observed that hypotonic stress resulted rapid tyrosine phosphorylation of a heterologous expression model native murine distal...
TRPV4, a nonselective cation channel of the transient receptor potential (TRP) family, is gated by hypotonicity. Expression TRPV4 mRNA has been detected in circumventricular organs brain responsible for sensing systemic tonicity and kidney distal convoluted tubule (DCT), among other sites. No analysis expression at protein level undertaken no systematic this reported kidney. Via RNAse protection assay immunoblotting, abundant was cortex, medulla, papilla. The pattern characterized both rat...
Bile reflux is a risk factor in the development of intestinal metaplasia stomach and believed to function as an initiator gastric carcinogenesis. However, whether G protein-coupled bile acid receptor TGR5 expressed this tumor not known. In study, we determined expression adenocarcinoma examined role cell proliferation. Strong staining was present 12% cases but no normal epithelium ( P < 0.01). Moderate strong membranous cytoplasmic 52% only 25% diffuse subtype adenocarcinomas 0.001)....
Inherited methylmalonic acidemia is characterized by mitochondrial dysfunction, oxidative stress, and damage of mitochondria-rich organs in children. It unclear whether acid (MMA) related to poor prognosis adults. The study aims investigate the associations MMA with all-cause cause-specific mortality general population. Overall, 23,437 adults from US National Health Nutrition Examination Survey (NHANES) were enrolled. NHANES 1999-2004 2011-2014 separately used as primary validation subsets...
Abstract Large rivers are the main arteries for transportation of carbon to ocean; yet, how hydrology and anthropogenic disturbances may change composition export dissolved organic matter along large river continuums is largely unknown. The Yangtze River has a watershed area 1.80 × 10 6 km 2 . It originates from Qinghai‐Tibet Plateau flows 6300 eastward through center China. We collected samples ( n = 271) continuum analyzed weekly at most downstream situated gauging station in 2017–2018...
The SLC30A8 gene codes for a pancreatic beta-cell-expressed zinc transporter, ZnT8. A polymorphism in the is associated with susceptibility to type 2 diabetes, although molecular mechanism through which this phenotype manifest incompletely understood. Such polymorphisms may exert their effect via impacting expression level of product. We used an shRNA-mediated approach reproducibly downregulate ZnT8 mRNA by >90% INS-1 beta cell line. ZnT8-downregulated cells exhibited diminished uptake...
Arsenic trioxide (ATO) is an effective therapeutic agent against acute promyelocytic leukemia (APL); however, its anti-tumor effect on solid tumors such as colorectal cancer (CRC) still in debate. Ascorbic acid (AA) also produces a selective cytotoxic activity tumor cells. Here, we exploit the potential benefit of ATO/AA combination generating cytotoxicity to CRC cells, which may lay ground work for combinational chemotherapy CRCs. According results, found that ATO and AA effectively...
Homocysteine (Hcy) is a recognized cardiovascular disease (CVD) risk factor linked with atherosclerosis. However, the association between Hcy and myocardial injury little known.
Adaptive radiotherapy (ART) provides greater benefits than intensity-modulated (IMRT) regarding dosimetric outcomes in patients with cervical cancer. To investigate the clinical of ART, we have collected data from 115 cancer who underwent radical at our institution. Fifty-nine received a single course IMRT. Fifty-six offline defined as reduction gross tumor volume (GTV) by least 30% after 30 Gy radiotherapy, followed modified treatment plan for second-stage. After treatment, 53 ART group...
Background Depressive and anxiety disorders constitute a major component of the disease burden mental in China. Aims To comprehensively evaluate depressive Method The raw data is sourced from Global Burden Disease, Injuries, Risk Factors Study (GBD) 2021. This study presented by prevalence disability-adjusted life years (DALYs) at both national provincial levels China 1990 to 2021, gender (referred as 'sex' GBD 2021) age. Results From number disorder cases (from 34.4 53.1 million) 40.5...
To evaluate the feasibility of multi-parameters combined simplified intensity-modulated radiation therapy (sIMRT) planning in thoracic tumors and provide guidance for clinical practice. A total 34 patients with who underwent radiotherapy during 2019 to 2020 our hospital were retrospectively analyzed. The same experienced medical physicist designed sIMRT planning. limited maximum number segments per beam-field, minimum segment area, monitor units (MU), remaining consistent conventional...
We identified a consensus N-linked glycosylation motif within the pore-forming loop between fifth and sixth transmembrane segments of osmoresponsive transient receptor potential (TRP) channel TRPV4. Mutation this residue from Asn to Gln (i.e., TRPV4 N651Q ) resulted in loss slower migrating band on anti-TRPV4 immunoblots marked reduction lectin-precipitable immunoreactivity. HEK293 cells transiently transfected with mutant exhibited increased calcium entry response hypotonic stress relative...
The prototypical member of the vanilloid-responsive-like subfamily transient receptor potential (TRP) channels is TRPV1. TRPV1 mediates aspects nociception and neurogenic inflammation; however, new roles are emerging in sensation both luminal stretch systemic tonicity. Although at least six nonsynonymous polymorphisms human gene have been identified, there has no systematic investigation into their functional consequences. When heterologously expressed HEK293 cells, all variants exhibited...
HYD-PEP06, an endostatin-modified polypeptide, has been shown to produce effective anti-colorectal carcinoma effects through inhibiting epithelial–mesenchymal transition (EMT). However, whether HYD-PEP06 similar suppressive effect on hepatocellular (HCC) remained unknown. In this study, inhibited metastasis and EMT but not proliferation in vitro. Cignal finder pathway reporter array Western blot analysis revealed that suppressed HCCLM3 cell by the PI3K/AKT pathway. Moreover, exerted...
A number of studies indicate that circular RNAs (circRNAs) play paramount roles in regulating the biological behavior glioblastoma multiforme (GBM). In this study, we investigated underlying mechanism circMELK GBM. Real-time PCRs were used to examine expression glioma tissues and normal brain (NBTs). Localization GBM cells was estimated by fluorescence <i>in situ</i> hybridization (FISH). Transwell migration three-dimensional invasion assays performed cell vitro</i>. Spheroid formation,...
This study was designed to investigate the effects and mechanism of action traditional Chinese drug formula, qiliqiangxin (QLQX), on cardiac function in spontaneously hypertensive rats (SHRs). We evaluated oral high-dose (4g/kg/day, n = 7) low-dose (1g/kg/day, QLQX SHRs aged between 8 compared control, 8-week-old Wistar–Kyoto (WKY) rats. Echocardiography performed evaluate hemodynamic parameters. Hematoxylin eosin (HE) Masson's trichrome staining were performed, expression myocardial...
The inconsistent relationship between Vitamin B12 (B12), methylmalonic acid (MMA, marker of deficiency) and mortality was poorly understood, especially in patients with coronary heart disease (CHD). This study aims to investigate the association serum MMA, B12-related biomarkers (serum level, dietary intake, supplement use, sensibility B12) all-cause cardiovascular adults CHD.The data this were from a subcohort within US National Health Nutrition Examination Survey (NHANES). We included...
The capsaicin receptor and transient potential channel TRPV1 senses heat, protons, vanilloid agonists in peripheral sensory ganglia. Abundant data have suggested the presence of potentially novel splice variants kidney. We report a rat variant, TRPV1(VAR), cloned from kidney papilla. TRPV1(VAR) cDNA was identified multiple tissues. Its sequence fully compatible with donor acceptor sites gene. is predicted to encode truncated form consisting NH2-terminal 248 residues (all within intracellular...
S100A12 belongs to the S100 family and acts as a vital regulator in different types of tumors. However, function thyroid carcinoma has not yet been investigated. In this study, we analyzed expression human papillary cancer (PTC) samples two PTC cell lines. addition, explored effects on progression vitro vivo. Our results showed that was significantly upregulated specimens. Moreover, silencing markedly inhibited proliferation, migration, invasion cycle progression. knockdown reduced CyclinD1,...
Abstract Circulating tumor cells (CTCs) are reported as the precursor of metastases, implying that stifling CTCs would be beneficial for metastasis prevention. However, challenges remain application therapies aim at due to lack effective CTC‐targeting strategy and sensitive therapeutic agents. Herein, a general CTC‐intervention based on neutrophil cyto‐pharmaceuticals is proposed suppressing CTC colonization formation. Breast cancer 4T1 infused mimic CTCs, trapped first elucidated in...