A5069969696- Epilepsy research and treatment
- Pharmacological Effects and Toxicity Studies
- Neuroscience and Neuropharmacology Research
- EEG and Brain-Computer Interfaces
- Memory and Neural Mechanisms
- Functional Brain Connectivity Studies
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Diet and metabolism studies
- Asthma and respiratory diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Fetal and Pediatric Neurological Disorders
- Neonatal and fetal brain pathology
- Allergic Rhinitis and Sensitization
- Neurological disorders and treatments
- Metabolism and Genetic Disorders
- Face Recognition and Perception
- Liver Disease Diagnosis and Treatment
- Cerebrospinal fluid and hydrocephalus
- Memory Processes and Influences
- Neurobiology of Language and Bilingualism
- Psychosomatic Disorders and Their Treatments
- Anesthesia and Sedative Agents
- Neural dynamics and brain function
University Medical Center Freiburg
2012-2025
University of Freiburg
2009-2025
University Hospital Regensburg
2022
SRH Hochschule für Gesundheit Gera
2019
Diakonie Kork
2017-2018
University of Lübeck
2018
University Hospital Heidelberg
2008-2018
Heidelberg University
2008-2018
Goethe University Frankfurt
2018
University Hospital Schleswig-Holstein
2018
Elevated levels of immunoglobulin (Ig) E are associated with bronchial asthma, a disease characterized by eosinophilic inflammation the airways. Activation antigen-specific T helper (Th) 2 cells in lung subsequent release interleukin (IL) 4 and IL-5 is believed to play an important role pathogenesis this disease. In study, we have used non-anaphylactogenic anti-mouse-IgE antibody investigate relationship between IgE, airway eosinophil infiltration, production Th2 cytokines. Immunization mice...
We have found that divalent electrolyte counterions common in biological cells (Ca(2+), Mg(2+), and Mn(2+) ) can condense anionic DNA molecules confined to two-dimensional cationic surfaces. DNA-condensing agents vivo include histones polyamines spermidine spermine with sufficiently high valence (Z) 3 or larger. In vitro studies show electrostatic forces between chains bulk aqueous solution containing remain purely repulsive, condensation requires counterion Z >/= 3. striking contrast...
The production of interleukin 2 (IL 2), IL 4 and interferon-gamma (IFN-gamma) by in vitro activated unselected human blood mononuclear cells was studied at a single-cell level. Individual lymphokine-synthesizing were identified intracellular immunofluorescent staining using cytokine-specific monoclonal or polyclonal antibodies. Cultures from adult donors revealed biphasic kinetic pattern for IFN-gamma with peaks occurring 4-6 24-30 h after initiation the cultures. Approximately 20%-40%...
There is a pressing need for immunosuppressants with an improved safety profile. The search novel approaches to blocking T-cell activation led the development of selective protein kinase C (PKC) inhibitor AEB071 (sotrastaurin). In cell-free assays inhibited PKC, <i>K</i><sub>i</sub> values in subnanomolar low nanomolar range. Upon stimulation, markedly situ PKCθ catalytic activity and selectively affected both canonical nuclear factor-κB factor activated T cells (but not activator protein-1)...
The definition of minimal standards remains pivotal as a basis for high standard care and staff allocation or reimbursement. Only limited publications are available regarding the required staffing methodologic expertise in epilepsy centers. executive board working group (WG) on presurgical diagnosis operative treatment published first guidelines 2000 Austria, Germany, Switzerland. In 2014, revised were WG decided to publish an unaltered English translation this report. Because surgery is...
Although the majority of children undergoing epilepsy surgery are younger than 3 yr at manifestation, only few actually receive surgical treatment in early childhood. Past studies have, however, suggested that earlier intervention may correlate with superior developmental outcomes.To identify predictors for long-term seizure freedom and cognitive development following first life determine appropriate timing this age group.We retrospectively analyzed data 48 consecutive aged 1.1 ± 0.7...
Zusammenfassung In der neuen S2k-Leitlinie „Erster epileptischer Anfall und Epilepsien im Erwachsenenalter“ werden Empfehlungen zu klinisch relevanten Fragestellungen in fünf großen Themenblöcken gegeben: Management erster Anfall, Pharmakotherapie, Epilepsiechirurgie, komplementäre supportive Therapieverfahren psychosoziale Aspekte. Beim Thema Leitlinie zunächst zur Unterscheidung beiden maßgeblichen Differenzialdiagnosen, Synkope psychogener nicht-epileptischer gegeben. Zudem wird...
The cooperative condensation of DNA and cationic liposomes to form ordered aggregates in aqueous solution is associated with the release partially bound counterions. We directly determine extent counterion by separating supernatant from precipitated condensates, measuring conductivity before after phase transition. calculated for a range lipid/DNA concentration ratios based on nonlinear Poisson−Boltzmann theory. Both experiment theory show maximal, essentially complete, counterions at...
Gender-related differences in brain activation patterns and their lateralization associated with cognitive functions have been reported the field of language, emotion, working memory. Differences hypothesized to be due different strategies. The aim present study was test whether hippocampi during memory processing differs between sexes. We acquired functional magnetic resonance imaging data from healthy female male participants performing a spatial task quantitatively assessed hippocampal...
To evaluate the seizure control and developmental outcomes after hemispherotomy for refractory epilepsy in childhood to identify their predictive factors.We retrospectively studied clinical courses of 52 children with who underwent Epilepsy Center Freiburg between 2002 2011.Mean age at onset was 1.8 years (range 0-8 years) mean surgery 6.7 6 months-18 years). The underlying etiology congenital 22 (42%) children, acquired 24 (46%), progressive (12%). At final follow-up 1-9.8 (mean 3.3), 43...
In contrast to the abundance of seizure outcome reports in epilepsy surgery for glioneuronal tumors childhood and adolescence, there is a dearth information regarding cognitive outcomes.To investigate children adolescents that underwent resective tumor-associated refractory determine their predictive factors.We retrospectively analyzed presurgical findings, resection types, outcomes over 1.3 12.3 years (mean, 7.3) 29 consecutive patients, who 2000 2011. The mean age at onset was 7.9 (range,...
Although frontal lobe resections account for one-third of intralobar in pediatric epilepsy surgery, there is a dearth information regarding long-term seizure freedom, overall cognitive and adaptive functioning.To identify outcome predictors define the appropriate timing surgery.We retrospectively analyzed data 75 consecutive patients aged 10.0 ± 4.9 yr at surgery that had an 8.1 mean follow-up.Etiology comprised focal cortical dysplasia (FCD) 71% benign tumors 16% cases. All but one magnetic...
Type III phosphatidylinositol-4-kinase beta (PI4KIIIβ) was previously implicated in hepatitis C virus (HCV) replication by small interfering RNA (siRNA) depletion and therefore proposed as a novel cellular target for the treatment of C. Medicinal chemistry efforts identified highly selective PI4KIIIβ inhibitors that potently inhibited genotype 1a 1b HCV replicons 2a vitro. Replicon cells required more than 5 weeks to reach low levels 3- 5-fold resistance, suggesting high resistance barrier...
Although epilepsy surgery is the only curative therapeutic approach for lesional drug-resistant (DRE), there reluctance to operate on infants due a fear of complications. A recent meta-analysis showed that in first 6 months life can achieve seizure control about two thirds children. However, robust data surgical complications and postoperative cognitive development are lacking. We performed retrospective multicenter study who underwent life. 15 at median age 134 days (IQR: 58) four centers....
Peripheral and central clonal deletion are important tolerance mechanisms in models using bone marrow transplantation (BMT) with costimulation blockade (CB). However, since can be found before peripheral is complete elimination of recipient CD4(+) cells at the time BMT prevents induction, we investigated potential roles regulation anergy such a murine model. We that transient CD25(+) or neutralization IL2 immediately after CB prevented induction skin graft tolerance. Cotransfer into SCID...
Abstract Interleukin (IL)‐4 and IL‐5 are two cytokines which synergize in the induction of several biological effector functions. They produced by mouse human T helper 2 (Th2) 0 (Th0) cells. Little is known about regulation at single‐cell level. Here we show, using a flow cytometric intracellular staining technique, that IL‐4 predominantly different peripheral CD4 + CD8 cells, whereas interferon (IFN)‐γ IL‐2 same In contrast, cloned Th0 Th2 cells were able to produce simultaneously. The...
The Wilms' tumour suppressor protein WT1 plays a central role in the development of kidney and also other organs. can act as transcription factor with highly context-specific activator repressor functions. We previously identified Brain Acid Soluble Protein 1 (BASP1) transcriptional cosuppressor that block activation function WT1. BASP1 are co-expressed during nephrogenesis both proteins ultimately become restricted to podocyte cells adult kidney. Here, we have analysed WT1/BASP1 complex...
Gelastic epilepsy due to hypothalamic hamartomas is usually a severe condition encompassing both epileptic seizures and an encephalopathy associated with behavioral cognitive impairments. Here we report the effects of interstitial radiosurgery in treatment this generally pharmacoresistant syndrome.Twenty-four consecutive patients (3-46 years age, 7 women, mean age 21.9 years, duration 17.6 years) gelastic MR-ascertained hamartoma minimum follow-up period 1 year were included evaluation....