Abstract Objectives To estimate the burden, trends, and inequalities of type 1 diabetes mellitus (T1DM) among older adults at global, regional, national level from 1990 to 2019. Design Population based study. Adults aged ≥65 years 21 regions 204 countries territories (Global Burden Disease Risk Factors Study 2019) Main outcome measures Primary outcomes were T1DM related age standardised prevalence, mortality, disability adjusted life (DALYs), average annual percentage change. Results The...

Objectives: Otitis media is one of the most important causes hearing loss at an early age. Effective vaccination with routine 7-valent pneumococcal conjugate vaccine (PCV-7) was introduced in 2000. It has been gradually replaced by nontypeable Haemophilus influenzae protein D or higher-valent 13-valent PCV (PCV-13) since 2010. Data on change otitis burden recent years are sparse global, regional, and national levels. Design: The Global Burden Disease 2019 study used to evaluate prevalence,...

Building agents with adaptive behavior in cooperative tasks stands as a paramount goal the realm of multi-agent systems. Current approaches to developing rely primarily on learning-based methods, whose policy generalization depends heavily diversity teammates they interact during training phase. Such reliance, however, constrains agents' capacity for strategic adaptation when cooperating unfamiliar teammates, which becomes significant challenge zero-shot coordination scenarios. To address...

Stroke is one of the leading causes death among children, yet evidence on stroke incidence and prognosis in this population largely neglected worldwide. The aim study was to estimate latest burden childhood stroke, as well trends, risk factors, inequalities from 1990 2019, at global, regional, national levels.

ABSTRACT The bacterium Haemophilus parasuis is the specific pathogenic cause of Glässer's disease in swine. Fifteen serotypes H. have been reported. A method to serotype isolates accurately would help prevent and control outbreaks through appropriate vaccination understand epidemiology geographic areas. However, according traditional serotyping, rate nontypeable (NT) strains 10 40%, which gives low accuracy. In present study, we developed a set PCR assays that are able identify all currently...

Abstract Background Reproductive performance of livestock is an economically important aspect global food production. The Chinese Meishan pig a prolific breed, with average three to five more piglets per litter than European breeds; however, the genetic basis for this difference not well understood. Results In study, we investigated copy number variations (CNVs) 32 pigs and 29 Duroc by next-generation sequencing. A genome-wide analysis 61 revealed 12,668 variable regions (CNVRs) that were...

Background and aim Despite the increasing rates of antibiotic resistance, furazolidone-based regimens have demonstrated promise in Helicobacter pylori eradication. Therefore, this study aims to evaluate comparative efficacy safety tetracycline versus amoxicillin quadruple therapy [bismuth with furazolidone (BQFT) vs. bismuth (BQFA)] rescue treatments.

Temperate savannas are globally distributed ecosystems that play a crucial role in regulating the global carbon cycle and significantly contribute to human livelihoods. This study aims develop novel method for identifying temperate map their distribution Northeastern China. To achieve this objective, Unmanned Aerial Vehicle (UAV) imagery was integrated with Sentinel-2 Sentinel-1 satellite using Random Forest (RF) regression Classification Regression Tree (CART) algorithms. The training...

Fractional vegetation cover (FVC) is an essential metric forvaluating ecosystem health and soil erosion. Traditional ground-measuring methods are inadequate for large-scale FVC monitoring, while remote sensing-based estimation approaches face issues such as spatial scale discrepancies between ground truth data image pixels, well limited sample representativeness. This study proposes a method integrating uncrewed aerial vehicle (UAV) satellite imagery using machine learning (ML) models....

Sustainable restoration of degraded ecosystems is a major environmental concern in several regions China. Changting one the severely affected water- and soil-loss areas southern China that have been under continuous management for last 30 years. Taking typical red soil erosion area Changting, Fujian, as research object, an evaluation index system with m resolution was developed based on Sensitivity–Resilience–Pressure (SRP) model. Spatial principal component analysis, Global Moran’s I, LISA...

Abstract Background and objectives Helicobacter pylori ( H. ) infection is the most common etiology of chronic gastric. gastritis would gradually evolve into gastric atrophy, intestinal metaplasia, dysplasia malignant lesions. Herein, this study aimed to investigate potential impact colonization density depth on severity histological parameters gastritis. Methods A prospective monocentric was conducted from December 2019 July 2022, enrolling patients with confirmed via histopathological...

Haemophilus parasuis is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) of H. potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis SC096 were identified (lgtB lex-1). Mutants constructed investigate the roles lgtB lex-1 genes. from ΔlgtB or Δlex-1 mutant showed truncated structure on silver-stained SDS-PAGE gel compared...

Adipose tissue plays a crucial role in energy metabolism. Several studies have shown that circular RNA (circRNA) is involved the regulation of fat development and lipid However, little known about their involvement adipogenic differentiation ovine stromal vascular fractions (SVFs). Here, based on previous sequencing data bioinformatics analysis, novel circINSR was identified sheep, which acts as sponge to promote miR-152 inhibiting SVFs. The interactions between were examined using...

The Guangling Large-Tailed sheep is renowned for its unique tail fat deposition, with a significant proportion of total body being localized in the region. Fat deposition complex biological process regulated by various molecular mechanisms. Our previous studies have identified large number differentially expressed circular RNAs (circRNAs) adipose tissue sheep. These circRNAs may play pivotal role deposition. Given potential regulatory functions metabolism, investigating their roles...

Lüliang black goat (LBG) is a unique livestock genetic resource of great significance for the local agriculture and economic development Shanxi, China. However, kidding rate LBG relatively low, which limits efforts to improve reproductive performance benefits this breed. Therefore, improving characteristics crucial increasing its benefits. In study, 20 does were selected whole-genome resequencing divided into two groups: 10 in T group (does with three consecutive kiddings twin kids) S single...

Embryonic mortality is a major concern in the commercial swine industry and primarily occurs early gestation, but also during mid-gestation (~ days 50-70). Previous reports demonstrated that embryonic loss rate was significant lower Meishan than breeds (including Duroc). Most studies have focused on little known about mid-gestation.In this study, protein expression patterns endometrial tissue from Duroc sows were examined mid-gestation. A total of 2170 proteins identified both breeds. After...

Embryo loss is a major factor affecting profitability in the pig industry. Embryonic mortality occurs during peri-implantation and mid-late gestation pigs. Previous investigations have shown that embryo rate Meishan pigs significantly lower than commercial breeds. Most studies focused on embryonic early gestation, but little known about losses gestation. In this study, we performed transcriptome analysis of endometrial tissue sows (gestation days 49 72) sampled from two breeds (Meishan (MS)...

In Haemophilus parasuis, the genes HAPS_0217 and HAPS_1695 are predicted to encode long-chain fatty acid-CoA ligases (FACSs). These proteins contain ATP/AMP signature motifs FACS conserved that homologous those in Escherichia coli FadD (EcFadD). this study, we demonstrate can functionally replace EcFadD E. fadD mutant JW1794, were thus designated fadD1 fadD2, respectively. An evaluation of kinetic parameters indicated FadD1 FadD2 have a substrate preference for acids. Moreover, exhibited...

Domestic goats are commonly reared for meat and milk production in several regions of the world. However, genetic mechanism underlying muscle development quality is limited. Therefore, aim this study was to identify known novel genes regulating using second- third-generation sequencing technologies. To achieve this, transcriptomes longissimus dorsi (LD) biceps femoris (BF) tissues Lingqiu Greyback were examined compared. Differentially expressed (DEGs) isoforms (DEIs) functionally annotated....

Adipose tissues represent an important energy storage organ in animals and are the largest endocrine organ. It plays regulatory role pathogenesis of insulin resistance, cardiovascular disease, metabolic syndrome. development is a complex biological process involving multiple key genes, signaling pathways, non-coding RNAs, including microRNAs circular RNAs. In this study, we characterized circITGB1 named its host gene ITGB1, which differentially expressed sheep different months based on...

Intrauterine growth restriction (IUGR) is a common perinatal complication in animal reproduction, with long-lasting negative effects on neonates and postnatal animals, which seriously negatively affects livestock production. In this study, we aimed to identify potential genes associated the diagnosis of IUGR through bioinformatics analysis. Based 73 differentially expressed related obtained by differential analysis weighted gene co-expression network analysis, used three machine learning...

Abstract MicroRNAs (miRNAs) regulate adipogenic differentiation in stromal vascular fractions (SVFs) through post‐transcriptional regulation of transcription factors and other functional genes. miR‐301 the homeobox C8 ( HOXC8 ) gene are involved lipid homeostasis; however, their roles ovine SVFs unknown. Here, we explored effects on regulatory role miR‐301a expression. Additionally, evaluated effect mRNA abundance markers ability to accumulate lipids. We found that regulates by directly...