A5101475762- Neurogenetic and Muscular Disorders Research
- Bone Metabolism and Diseases
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Prenatal Screening and Diagnostics
- RNA Research and Splicing
- Osteoarthritis Treatment and Mechanisms
- Retinal Diseases and Treatments
- Vitamin C and Antioxidants Research
- Renal and related cancers
- Pharmaceutical studies and practices
- Hippo pathway signaling and YAP/TAZ
- Muscle Physiology and Disorders
- Hedgehog Signaling Pathway Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- Attention Deficit Hyperactivity Disorder
- Fetal and Pediatric Neurological Disorders
- Genetic Syndromes and Imprinting
- Parvovirus B19 Infection Studies
- Folate and B Vitamins Research
- Cancer Risks and Factors
- Angiogenesis and VEGF in Cancer
Gansu University of Traditional Chinese Medicine
2022-2025
Peking University First Hospital
2021-2024
Peking University
2021-2024
Zhejiang University
2014-2024
Second Affiliated Hospital of Nanjing Medical University
2018-2024
Sir Run Run Shaw Hospital
2023
First Affiliated Hospital Zhejiang University
2023
Hangzhou City University
2023
Wuhan University
2022-2023
Renmin Hospital of Wuhan University
2022-2023
Knee osteoarthritis (KOA) represents a progressive degenerative disorder characterized by the gradual erosion of articular cartilage. This study aimed to develop and validate biomarker-based predictive models for KOA diagnosis using machine learning techniques. Clinical data from 2594 samples were obtained stratified into training validation datasets in 7:3 ratio. Key clinical features identified through differential analysis between control groups, combined with least absolute shrinkage...
We have recently found that diabetes-induced premature senescence of retinal endothelial cells is accompanied by NOX2-NADPH oxidase-induced increases in the ureohydrolase enzyme arginase 1 (A1). Here, we used genetic strategies to determine specific involvement A1 cell senescence. knockout mice and wild type were rendered diabetic with streptozotocin (ECs) exposed high glucose or transduced adenovirus overexpress for these experiments. ABH [2(S)-Amino-6-boronohexanoic acid] was inhibit...
Cdc42, a member of Rho family small guanosine triphosphatases (GTPases), is critical for cartilage development. We investigated the roles Cdc42 in osteoarthritis and explored potential mechanism underlying Cdc42-mediated articular degeneration subchondral bone deterioration. highly expressed both mouse model with surgical destabilization medial meniscus (DMM) knee joints. Specifically, genetic disruption knockdown expression, or inhibition activity robustly attenuates DMM-induced...
The diagnosis of intellectual disability/developmental delay (ID/DD) benefits from the clinical application target/exome sequencing. yield in Mendelian diseases varies 25% to 68%. aim present study was identify genetic causes 33 ID/DD patients using Recent studies have demonstrated that reanalyzing undiagnosed exomes could additional diagnosis. Therefore, addition normal data analysis, this study, re‐evaluation performed prior manuscript preparation after updating OMIM annotations, calling...
Abstract Within the vascular endothelial growth factor (VEGF) family of five subtypes, VEGF165 secreted by cells has been identified to be most active and widely distributed that plays a vital role in courses angiogenesis, vascularization mesenchymal cell differentiation. Hair follicle stem (HFSCs) can harvested from bulge region outer root sheath hair are adult have multi‐directional differentiation potential. Although research on (such as fat bone marrow cells) extensive, but various...
The Wnt/β-catenin signaling pathway appears to be particularly important for bone homeostasis, whereas nuclear accumulation of β-catenin requires the activation Rac1, a member Rho small GTPase family. aim present study was investigate role RhoA/Rho kinase (Rock)-mediated in regulation aging-associated loss. We find that Lrp5/6-dependent and Lrp5/6-independent RhoA/Rock by Wnt3a activates Jak1/2 directly phosphorylate Gsk3β at Tyr216, resulting subsequent destabilization. In line with these...
Abstract Inhibitors of phosphodiesterase 5 (PDE5) are widely used to treat erectile dysfunction and pulmonary hypertension in clinics. PDE5, cyclic guanosine monophosphate (cGMP), protein kinase G (PKG) important components the non-canonical Wnt signaling. This study aimed investigate effect PDE5 inhibition on canonical signaling osteoblastogenesis, using both vitro cell culture vivo animal models. In experiments, resulted activation cGMP-dependent 2 consequent glycogen synthase 3 β...
Osteoarthritis (OA) is a degenerative disease of articular cartilage involving the entire joint tissue. Columbianetin (CBT) major active compound radix angelicae pubescentis, which used in treatment OA. This paper attempts to explore role CBT Lipopolysaccharides (LPS) was induce mouse chondrocytes ATDC5. The effect on cell viability ATDC5 cells with or without LPS induction determined by CCK-8 and LDH kits. inflammatory response evaluated using ELISA Apoptosis LPS-induced were examined TUNEL...
To explore and evaluate new malignant predictors of breast non-mass enhancement lesions using the BI-RADS MRI lexicon.A dataset involving 422 consecutive women underwent 3.0 T between January 2014 July 2016 was assembled for this study. Each case retrospectively reviewed by 3 radiologists. Eighty-four that present in 79 patients were identified Dynamic contrast-enhanced features analyzed univariate multivariate analyses to identify significant indicators malignancy.Of 84 lesions, 52 (61.9%)...
Abstract Aberrant activation of sonic hedgehog (SHH) signaling and its effector transcriptional factor GLI1 are essential for oncogenesis SHH-dependent medulloblastoma (MB SHH ) basal cell carcinoma (BCC). Here, we show that inactivates p38α (MAPK14) in a smoothened-dependent manner, conversely, directly phosphorylates on Ser937/Ser941 (human/mouse) to induce GLI1’s proteasomal degradation negates the transcription signaling. As result, Gli1 S941E loss-of-function knock-in significantly...
Abstract Objectives The objective of this study is to report 6‐year clinical prenatal diagnosis experience Duchenne muscular dystrophy (DMD)‐affected families evaluated at a single center in China and establish reliable rational procedure for DMD families. Methods data 146 at‐risk pregnancies 131 referred our from 2010 2016 were retrospectively reviewed. Results mutation detection rate the probands was greater than 99%. In families, 50 mothers showed negative results during carrier testing,...
Background Duchenne and Becker muscular dystrophy (DMD/BMD) are X‐linked recessive disorders caused by mutation in dystrophin gene. We reported 3‐year clinic experience from a single hospital Shanghai using multiplex ligation dependent probe amplification (MLPA) assay to detect DMD mutations. Methods Four hundred fifty‐one males 184 females, who were clinically diagnosed as DMD/BMD patients or carriers at our hospital's outpatient clinic, collected performed with MLPA gene Results Seventeen...
This study reported the clinical prenatal diagnosis experience of families affected by methylmalonic acidemia (MMA) evaluated at a single center over 8 years, and reliability biochemical approach for was analyzed.Prenatal data 187 MMA referred to our from 2009 2016 were reviewed retrospectively. The results genetic analysis compared.A total 41 MMA-affected pregnancies (21%) identified. could identify true status 99.5% fetuses. diagnostic sensitivities propionylcarnitine (C3) level, C3...