A5005030428- Pancreatic function and diabetes
- Bacterial Infections and Vaccines
- Immunodeficiency and Autoimmune Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Inflammatory Bowel Disease
- CRISPR and Genetic Engineering
- Pneumonia and Respiratory Infections
- Nutrition, Genetics, and Disease
- Herpesvirus Infections and Treatments
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Microplastics and Plastic Pollution
- Liver Disease Diagnosis and Treatment
- Diabetes Treatment and Management
- Adipose Tissue and Metabolism
- Effects and risks of endocrine disrupting chemicals
- Diet, Metabolism, and Disease
- Cancer, Lipids, and Metabolism
- Antioxidant Activity and Oxidative Stress
- Iron Metabolism and Disorders
- Genomics and Rare Diseases
- Mycobacterium research and diagnosis
- Folate and B Vitamins Research
- Infective Endocarditis Diagnosis and Management
- RNA modifications and cancer
Centre National de la Recherche Scientifique
2013-2020
Inserm
2020
European Genomic Institute for Diabetes
2013-2020
Institut Pasteur de Lille
2013-2020
Université de Lille
2013-2020
(Epi)génomique fonctionnelle métabolique et des dysfonctions dans le diabète de type 2 et des maladies associées
2019
Centre Hospitalier Universitaire de Lille
2017
Activation of the p53 pathway in adipose tissue contributes to insulin resistance associated with obesity. However, mechanisms activation and effect on adipocyte functions are still elusive. Here we found a higher level DNA oxidation reduction telomere length mice fed high-fat diet an increase damage adipocytes. Interestingly, hallmarks chronic visible at onset Furthermore, injection lean doxorubicin, damage-inducing drug, increased expression chemokines promoted its infiltration by...
OBJECTIVE Accurate etiological diagnosis of monogenic forms diabetes and obesity is useful as it can lead to marked improvements in patient care genetic counseling. Currently, molecular based on Sanger sequencing restricted only a few genes, this technology expensive, time-consuming, labor-intensive. High-throughput next-generation (NGS) provides an opportunity develop innovative cost-efficient methods for sensitive multigene screening. RESEARCH DESIGN AND METHODS We assessed new method PCR...
Bisphenol A (BPA) exposure has been suspected to be associated with deleterious effects on health including obesity and metabolically-linked diseases. Although bisphenols F (BPF) S (BPS) are BPA structural analogs commonly used in many marketed products as a replacement for BPA, only sparse toxicological data available yet. Our objective was comprehensively characterize gene targets human primary adipocyte model, order determine whether they may induce cellular dysfunction, using chronic at...
Gastrointestinal adverse effects occur in 20-30% of patients with metformin-treated type 2 diabetes, leading to premature discontinuation 5-10% the cases. intolerance may reflect localized high concentrations metformin gut. We hypothesized that reduced transport via plasma membrane monoamine transporter (PMAT) and organic cation 1 (OCT1) could increase risk severe gastrointestinal effects.The study included 286 metformin-intolerant 1,128 metformin-tolerant individuals from IMI DIRECT...
The population of Guadeloupe Island exhibits a high prevalence obesity. We aimed to investigate whether rare genetic mutations in genes involved monogenic obesity (or diabetes) might be causal this Afro-Caribbean ancestry. This was secondary analysis study on conducted schoolchildren from 2013 that assess changes children's profiles after lifestyle intervention program. Through next-generation sequencing, we sequenced coding regions 59 or diabetes participants study. A total 25 obese were...
Excess liver iron content is common and linked to the risk of hepatic extrahepatic diseases. We aimed identify genetic variants influencing use genetics understand its link other traits
Molecular diagnosis of monogenic diabetes and obesity is paramount importance for both the patient society, as it can result in personalized medicine associated with a better life eventually saves health care spending. Genetic clinical laboratories are currently switching from Sanger sequencing to next-generation (NGS) approaches but choosing optimal protocols not easy. Here, we compared coverage 43 genes involved forms obesity, variant detection rates, resulting four enrichment methods...
The NOD2 gene, involved in innate immune responses to bacterial peptidoglycan, has been found be closely associated with Crohn's Disease (CD), an Odds Ratio ranging from 3⁻36. Families three or more CD-affected members were related a high frequency of gene variations, such as R702W, G908R, and 1007fs, reported the EPIMAD Registry. However, some rare CD multiplex families described without identification common linked-to-disease variations. In order identify new genetic variation(s) linked...
The whooping cough agent Bordetella pertussis coordinately regulates the expression of its virulence factors with two-component system BvgAS. In laboratory conditions, specific chemical modulators are used to trigger phenotypic modulation B. from default virulent Bvg+ phase avirulent Bvg- or intermediate Bvgi phases, in which no only a subset them produced, respectively. Whether occurs host remains unknown. this work, recombinant strains harboring BvgS variants were tested mouse model...
The whooping cough agent Bordetella pertussis controls the expression of its large virulence regulon in a coordinated manner through two-component signal transduction system BvgAS. In addition to genes coding for bona fide factors, Bvg comprises unknown function. this work, we characterized new Bvg-activated gene called BP2936. Homologs BP2936 are found other pathogenic Bordetellae and several species, including plant pathogens environmental bacteria. We showed that product is...
The NOD2 gene, involved in innate immune responses to bacterial peptidoglycan, has been found be strongly associated with Crohn’s Disease, an Odd Ratio ranging from 3 36. Families or more CD affected patients were related high frequency of gene variations as R702W, G908R, 1007fs and reported EPIMAD Registry. However, some rare multiplex families described without identification common linked-to-disease variations. In order identify new genetic variation(s) a major effect on disease...