A5014361516- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory Support and Mechanisms
- Inhalation and Respiratory Drug Delivery
- COVID-19 Clinical Research Studies
- Antimicrobial Resistance in Staphylococcus
- Long-Term Effects of COVID-19
- Bacteriophages and microbial interactions
- Asthma and respiratory diseases
- Tuberculosis Research and Epidemiology
- High Altitude and Hypoxia
- Heart Rate Variability and Autonomic Control
- Renal function and acid-base balance
- SARS-CoV-2 and COVID-19 Research
- Pulmonary Hypertension Research and Treatments
- Obstructive Sleep Apnea Research
- Nitric Oxide and Endothelin Effects
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Biochemical and Structural Characterization
- Tracheal and airway disorders
- Respiratory and Cough-Related Research
- Neuroscience of respiration and sleep
- Microbial Metabolism and Applications
- Bacterial biofilms and quorum sensing
- Helicobacter pylori-related gastroenterology studies
International University of Health and Welfare
2014-2025
Fujita Health University
2020-2024
General Electric (Japan)
2023
Juntendo University Urayasu Hospital
2021
Japan Anti Tuberculosis Association
2021
Kaohsiung Chang Gung Memorial Hospital
2018
Kawasaki Heavy Industries (Japan)
2011
Yamaguchi University
2007
National Center For Child Health and Development
2004
Institut Pasteur
2002
Tobacco smoke is believed to cause small airway disease and then chronic obstructive pulmonary (COPD), but the molecular mechanisms by which obstruction occurs remain unknown. To study gene expression levels of transforming growth factor (TGF)-beta1, a potent fibrogenic factor, in epithelium from smokers patients with COPD, we harvested highly pure samples epithelial cells airways under direct vision using an ultrathin bronchofiberscope BF-2.7T (outer diameter 2.7 mm biopsy channel 0.8...
Asthma is a heterogeneous disease composed of various phenotypes. Periostin, molecule inducible with interleukin (IL)-4 or IL-13 in bronchial epithelial cells, biomarker "TH2-high" asthma. The objective this study to examine whether the serum periostin concentrations are correlated severity, specific phenotype(s), comorbidity Serum were measured 190 Japanese asthmatic patients and 11 healthy controls. protocol was registered under UMIN 000002980 clinical trial registry. significantly higher...
Surmmary Colonisation by Staphylococcus aureus in the nares of 120 outpatients and 63 healthy adults was studied for c. 2 years. Two states carriage S. were confirmed: Persistent persistent non-carriage. The non-carriage quite stable > 60% population any study groups non-carriers. results typing strains isolated from same individuals at different times with DNA fingerprinting digestion Smal enzyme showed that all carriers persistently infected strain changes seldom occurred.
To study the inflammatory responses of small-airway epithelium in smokers, we harvested enough living epithelial cells (1.97 × 10 6 ± 0.74 ) with a new ultrathin fiberscope from very peripheral airways 22 current smokers and 17 subjects who never smoked after informed consent was obtained. The were keratin positive composed mainly nonciliated cells. expression levels markers [interleukin (IL)-8 intercellular adhesion molecule (ICAM)-1] evaluated RT-PCR. magnitude mRNA corrected by β-actin...
Chitin concentrations greater than 0.04% (wt/wt) protected cholera vibrios against killing at low temperature. This protective effect was detected with both the soluble form of chitin, glycol and insoluble particulate chitin. Some amino acids or peptides also showed same effect.
The fine structure of the Staphylococcus aureus cell wall was determined by electron microscopy with new technique rapid freezing and substitution fixation. surface covered a fuzzy coat which consisted fibers or an electron-dense mass. Morphological examination wall, treated sequentially sodium dodecyl sulfate, trypsin, trichloroacetic acid, revealed that this partially removed trypsin digestion completely acid extraction but not affected sulfate treatment, suggesting consists mostly complex...
Journal Article Association of Vibrio cholerae O1 with the cyanobacterium, Anabaena sp., elucidated by polymerase chain reaction and transmission electron microscopy Get access M.S. Islam, Islam 1International Centre for Diarrhoeal Disease Research, Bangladesh, GPO Box 128, Dhaka-1000, Bangladesh Address correspondence: Dr Md Sirajul Environmental Microbiology Laboratory, ICDDR,B, Bangladesh; phone +880 2 871751 60 ext. 2405/2407, fax 872529 883116. sislam@icddrb.org Search other works this...
The arrangement of peptidoglycan in the cell wall Staphylococcus was observed with newly developed freeze-fracture technique, using n-octanol instead water as freezing medium. replica trichloroacetic acid-extracted (TCA-wall) showed two areas. One them has a concentric circular structure, characteristic surface structure staphylococcal wall, and other an irregular rough surface. chemical analysis revealed that TCA-wall consisted mostly peptidoglycan. By digesting lysozyme, structures were...
Introduction The rapid spread of COVID-19 posed a global burden. Substantial number people died the disease in acute phase infection. In addition, significant proportion patients have been reported to suffer from post-acute symptoms, sequelae COVID-19, which may negatively influence quality daily living and/or socioeconomic circumstances patients. However, no previous study has comprehensively and objectively assessed life by using existing international scales. Further, evidence...
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Abstract: Hepatopulmonary syndrome (HPS) (hypoxaemia due to intrapulmonary vasodilation and a right‐to‐left shunt associated with liver disease) resolves after transplantation. The authors describe case of spontaneous resolution HPS prior This patient was diagnosed extra‐hepatic biliary atresia when she 10 years old. She exhibited digital clubbing, facial vascular dilation, cyanosis, suffered from dyspnoea during exercise. patient's PaO 2 at rest 53.8 mm Hg in room air Technetium‐99m...
The surface of several laboratory strains Staphylococcus aureus were observed with a scanning electron microscope, and the presence two morphologically characteristic structures--a ridge separating cell into old new surfaces concentric circular structure--are described. These structures seemed to be present universally on cells genus Staphylococcus. removal by mild treatment trichloroacetic acid suggested that this structure represent circularly arranged teichoic acid. With experiments using...
Aims: To identify an extreme thermophile, strain TMY, isolated from silica scale the geothermal electric power plant and to examine microdiversity of Thermus thermophilus strains. Materials Results: The TMY was identified by morphological, biochemical physiological tests. Phylogenetic comparison other strains with 16S rDNA analysis, RAPD ERIC-PCR fingerprinting were performed. Strain closely related which a hot spring in New Zealand shown belong Japanese cluster. However, there considerable...
A meta-analysis suggested that the use of varenicline, which is a partial agonist nicotinic acetylcholine receptors and effective in smoking cessation, increases risk cardiovascular events within 52 weeks starting treatment. Defining these as occurring during drug treatment (usually for 12 weeks) or 30 days discontinuation, another showed was statistically insignificant. In present study, we aimed to clarify effect varenicline-assisted cessation on vascular endothelial function assessed by...
<h3>Background:</h3> Systemic sclerosis (Scleroderma; SSc) is a rare autoimmune connective tissue disease characterized by vasculopathy and fibrosis leading to progressive dysfunction of multiple organs. In 90 % patients with SSc, the upper gastrointestinal tract typically involved especially in esophageal lesions such as peristalsis and/or gastroesophageal reflux (GERD) due esophagus. However whether dysphagia an unique organ involvement or associated SSc itself still unclear, since there...
Electron microscopic studies of the surface structure Campylobacter fetus by freeze-etching method showed two different types S layer. One was in a hexagonal array, and other tetragonal array. A high-passage-number strain lost its layer during cultivation on culture media but regained it after single animal passage.
Graphical Abstract Gastrointestinal (GI) long-COVID symptoms, including diarrhea and abdominal pain, have been reported in patients with long-COVID. However, the clinical features of GI symptoms remain unclear. We conducted a large-scale prospective cohort study focusing on characteristics Japan. Among 943 COVID-19 patients, 58 (6.2%) had symptoms. The health-related quality life (QOL) parameters (the Short Form-8 [SF-8] Euro Quality Life 5 Dimensions level [EQ-5D-5L]) at 12 months after...
Alterations of the p53 tumor suppresser gene have been observed in a variety human and mouse tumors. For investigation role this tumors cats, feline cDNA was molecularly cloned by PCR amplifications using primers based on sequences conserved among several species. The appeared to cover approximately 90% open reading frame had characteristic structures common with genes other amino acid sequence similarities human, mouse, rat chicken counterparts were 82.9%, 75.6%, 76.5% 57.2% respectively....
In Japan, which ranks first among developed countries in the incidence of tuberculosis, intestinal tuberculosis should be regarded as an important disease. However, few studies this condition have recently been reported. We analyzed nine patients treated at our hospital during and after 2000. Our results differ from those previous that many were healthy, not elderly, had primary presented with non-gastrointestinal symptoms. All seven tested found to whole blood interferon gamma release assay...