Kazumi Nishio
A5082805006- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Long-Term Effects of COVID-19
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Pulmonary Hypertension Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Thermal Regulation in Medicine
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Diabetes and associated disorders
- Pleural and Pulmonary Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- High Altitude and Hypoxia
- Cell death mechanisms and regulation
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Mechanical Circulatory Support Devices
- Eicosanoids and Hypertension Pharmacology
- Amoebic Infections and Treatments
Kawasaki Municipal Hospital
2011-2025
Keio University
1995-2004
Kitasato Institute Hospital
1997-2003
Zhejiang Medicine (China)
2003
Keio University Hospital
1998
Taisho Pharmaceutical (Japan)
1998
National Hospital Organization Saitama Hospital
1995
Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge1-5. Here we conducted a genome-wide association study (GWAS) involving 2,393 cases of in cohort Japanese individuals collected during initial waves pandemic, with 3,289 unaffected controls. We identified variant on chromosome 5 at 5q35 (rs60200309-A), close to dedicator cytokinesis 2 gene (DOCK2), which was associated patients less than 65 years age. This risk allele prevalent East Asian but rare...
Mechanisms underpinning the dysfunctional immune response in severe acute respiratory syndrome coronavirus 2 infection are elusive. We analyzed single-cell transcriptomes and T B cell receptors (BCR) of >895,000 peripheral blood mononuclear cells from 73 disease 2019 (COVID-19) patients 75 healthy controls Japanese ancestry with host genetic data. COVID-19 showed a low fraction nonclassical monocytes (ncMono). report downregulated transitions classical to ncMono reduced CXCL10 expression...
Although permissive hypercapnia improves the prognosis of patients with acute respiratory distress syndrome, it has not been conclusively determined whether hypercapnic acidosis (HA) is harmful or beneficial to sustained inflammation lung. The present study was designed explore molecular mechanism HA in modifying lipopolysaccharide (LPS)-associated signals pulmonary endothelial cells. LPS elicited degradation inhibitory protein kappaB (IkappaB)-alpha, but IkappaB-beta, resulting activation...
Abstract Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious that has caused millions of deaths, where comprehensive understanding mechanisms still unestablished. In particular, studies gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on thorough analysis whole blood RNA-seq data from 465 genotyped samples the Japan Task Force, including 359 severe 106 non-severe cases. We discover 1169 putative causal...
Abstract Although c-Jun NH2-terminal kinase (JNK) has been implicated in the pathogenesis of transplantation-induced ischemia/reperfusion (I/R) injury various organs, its significance lung transplantation not conclusively elucidated. We therefore attempted to measure transitional changes JNK and AP-1 activities I/R-injured lungs. Subsequently, we assessed effects inhibition by three agents including SP600125 on degree means biological markers bronchoalveolar lavage fluid histological...
Multiple prolonged symptoms are observed in patients who recover from an acute COVID-19 infection, which is defined as long COVID. General fatigue frequently with COVID during and post-acute phases. This study aimed to identify the specific risk factors for general
Abstract Asthma is characterized by acute and chronic airway inflammation, the severity of hyperreactivity correlates with degree inflammation. Many features lung inflammation observed in human asthma are reproduced OVA-sensitized/challenged mice. T lymphocytes, particularly Th2 cells, critically involved genesis allergic response to inhaled Ag. In addition antiapoptotic effects, broad-spectrum caspase inhibitors inhibit cell activation vitro. We investigated effect inhibitor,...
To elucidate the differential role of various adhesion molecules in distorting leukocyte behavior microvasculature hyperoxia-exposed rat lungs, we investigated fluorescein-labeled and erythrocyte kinetics isolated lungs taken from animals exposed to 90% O2 for 48 h under conditions which endothelial intercellular molecule-1 (ICAM-1) P-selectin were inhibited by appropriate monoclonal antibodies (1A29 ICAM-1 ARP2-4 P-selectin), while L-selectin was restrained with fucoidin. Measurements blood...
Intercellular adhesion molecule-1 (ICAM-1) of the vascular endothelium plays a key role in development pulmonary oxygen toxicity. We studied effect steroid on hyperoxia-induced ICAM-1 expression using cultured endothelial cells vitro. Human artery (HPAECs) were to confluence, and then monolayers exposed either control (21% O(2)-5% CO(2)) or hyperoxic (90% conditions with without synthetic glucocorticoid, methylprednisolone (MP). MP reduced mRNA dose-dependent manner. Neutrophil...
To assess the importance of endothelial intercellular adhesion molecule-1 (ICAM-1) in microvascular leukocyte kinetics diseased lungs, we investigated transitional changes ICAM-1 expression, vascular diameter, and behavior rat pulmonary microcirculation during development acute lung injury (ALI) chronic fibrosis (FIB) evoked by bleomycin (BLM). Observations were made isolated perfused with a real-time confocal laser luminescence microscope. Microvascular cell evaluated measuring...
Abstract —To elucidate the differential reactivity of pulmonary microvessels in acini to hypoxia, excessive CO 2 , and increased H + we investigated changes diameter precapillary arterioles, postcapillary venules, capillaries isolated rat lungs on exposure normocapnic hypoxia (2% O ), normoxic hypercapnia (15% isocapnic acidosis (0.01 mol/L HCl). Microvascular diameters were precisely examined using a real-time confocal laser scanning luminescence microscope coupled high-sensitivity camera...
Although the endothelial expression of various adhesion molecules substantially differs between pulmonary microvessels, their importance for neutrophil and lymphocyte sequestration in ventilator-induced lung injury (VILI) has not been systematically analyzed. We investigated kinetics polymorphonuclear cells (PMN) mononuclear (MN) acinar microcirculation isolated rat with VILI by real-time confocal laser fluorescence microscopy, or without inhibition ICAM-1, VCAM-1, P-selectin monoclonal...
Introduction The rapid spread of COVID-19 posed a global burden. Substantial number people died the disease in acute phase infection. In addition, significant proportion patients have been reported to suffer from post-acute symptoms, sequelae COVID-19, which may negatively influence quality daily living and/or socioeconomic circumstances patients. However, no previous study has comprehensively and objectively assessed life by using existing international scales. Further, evidence...
Abstract To elucidate the host genetic loci affecting severity of SARS-CoV-2 infection, or Coronavirus disease 2019 (COVID-19), is an emerging issue in face current devastating pandemic. Here, we report a genome-wide association study (GWAS) COVID-19 Japanese population led by Japan Task Force, as one initial discovery GWAS studies performed on non-European population. Enrolling total 2,393 cases and 3,289 controls, not only replicated previously reported risk variants (e.g., LZTFL1, FOXP4,...
This study aimed to examine the behavior of stimulated leukocytes in pulmonary microcirculation. The leukocyte-endothelium interaction was visualized under physiological shear rates perfused rat lungs using high-speed confocal laser video microscopy. Leukocytes labeled with carboxyfluorescein were cytokine-induced neutrophil chemoattractant (CINC/gro), which caused L-selectin shedding and inverse upregulation CD18. Neither unstimulated nor exhibited rolling either arterioles or venules,...
Rationale: The clinical features and prognosis of nontuberculous mycobacterial (NTM) pleuritis pleural effusion combined with NTM lung disease remain unclear. Objectives: To investigate the pleuritis. Methods: This retrospective observational study included patients from January 2001 to June 2018 across eight hospitals in Japan. was defined by a positive culture samples. We matched Mycobacterium avium complex (MAC) (MAC-LD) without sex age obtain comparative data investigated association...
To investigate the pathogenesis of pulmonary oxygen toxicity, we examined effect hyperoxia on adhesion molecule expression in cultured human artery endothelial cells (HPAEC) and umbilical vein (HUVEC). Endothelial cell monolayers were exposed to either hyperoxic (90% O(2)-5% CO(2)) or normoxic (21% conditions for various periods. The level intercellular (ICAM)-1 had increased hyperoxia-exposed HPAEC HUVEC at 48 h (194 +/- 38 233 56%, respectively; P < 0.001) 72 (200 43 223 52%, compared with...
The hydrogen ion is an important factor in the alteration of vascular tone pulmonary circulation. Endothelial cells modulate by producing vasoactive substances such as prostacyclin (PGI2) through a process depending on intracellular Ca2+ concentration ([Ca2+]i). We studied influence CO2-related pH changes [Ca2+]i and PGI2 production human artery endothelial (HPAECs). Hypercapnic acidosis appreciably increased from 112 +/- 24 to 157 38 nmol/l. Intracellular acidification at normal...
To assess the effects of exposure lung to hyperoxic conditions on reactivity pulmonary microcirculation hypoxic stimulation, we measured hypoxia-elicited overall pressor changes (HPV) and microvascular diameter in intraacinar arterioles, venules, capillaries isolated perfused rat lungs exposed a environment (90% O2). estimate importance vasoactive prostaglandins nitric oxide (NO) for HPV modification, examined roles constitutive inducible forms cyclooxygenase (COX-1 COX-2) those NO synthase...
症例は64歳，女性．咳嗽を主訴に近医受診．胸部CT上，多発小結節影および気管支拡張像が認められ，当院紹介受診した．気管支洗浄液にて抗酸菌陽性となり16S rRNAおよびrpoB遺伝子の相同性からMycobacteruim shinjukuenseと同定された．本菌株はTranscription Reverse-transcription Concerted method（TRC法）で結核菌群偽陽性を示した．クラリスロマイシン，リファンピシン,エタンブトール3剤併用療法を開始し，画像所見および自覚症状の改善が得られた．