A5100418915- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Plant responses to water stress
- Plant Reproductive Biology
- Silicon Effects in Agriculture
- Plant Gene Expression Analysis
- Hepatitis C virus research
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Cancer-related molecular mechanisms research
- Legume Nitrogen Fixing Symbiosis
- Hepatitis B Virus Studies
- Photosynthetic Processes and Mechanisms
- Plant Physiology and Cultivation Studies
- Liver Disease Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Plant Disease Resistance and Genetics
- Cytomegalovirus and herpesvirus research
- Marine and coastal ecosystems
- Yeasts and Rust Fungi Studies
- Horticultural and Viticultural Research
- Powdery Mildew Fungal Diseases
Kunming University of Science and Technology
2014-2025
State Ethnic Affairs Commission
2025
Chongqing Medical University
2021-2025
Second Affiliated Hospital of Chongqing Medical University
2021-2025
Dalian Medical University
2021-2025
Jiangsu University of Science and Technology
2018-2024
Anhui Agricultural University
2016-2024
Second Xiangya Hospital of Central South University
2024
Central South University
2021-2024
Sericultural Research Institute
2018-2024
The interface between cellular systems involving small noncoding RNAs and epigenetic change remains largely unexplored in metazoans. RNA-induced silencing have the potential to target particular regions of genome for by locating specific sequences recruiting chromatin modifiers. Noting that several genes encoding RNA components been implicated regulation Drosophila , we sought a direct link system heterochromatin components. Here show PIWI, an ARGONAUTE/PIWI protein family member binds...
The recently acquired archaeological record for soybean from Japan, China and Korea is shedding light on the context in which this important economic plant became associated with people was domesticated. This paper examines (charred) seed size variation to determine what insight can be gained a comprehensive comparison of 949 specimens 22 sites. Seed length alone appears represent change through time, although length×width×thickness product has potential provide better resolution. A...
piRNAs are a class of small RNAs that is most abundantly expressed in the animal germ line. Presently, substantial research going on to reveal functions epigenetic and post-transcriptional regulation transposons genes. A piRNA database for collection, annotation structuring these data will be valuable contribution field, we have therefore developed piRBase platform which integrates various piRNA-related high-throughput data. has largest collection among existing databases, contains at...
3' uridylation is increasingly recognized as a conserved RNA modification process associated with turnover in eukaryotes. 2'-O-methylation on the terminal ribose protects micro(mi)RNAs from truncation and Arabidopsis. Previously, we identified HESO1 nucleotidyl transferase that uridylates most unmethylated miRNAs vivo, but substantial tailing of still remains heso1 loss-of-function mutants. In this study, found among nine other potential transferases, UTP:RNA URIDYLYLTRANSFERASE 1 (URT1)...
An 8× draft genome was obtained and annotated for Ralstonia solanacearum race 3 biovar 2 (R3B2) strain UW551, a United States Department of Agriculture Select Agent isolated from geranium. The UW551 consisted 80,169 reads resulting in 582 contigs containing 5,925,491 base pairs, with an average 64.5% GC content. Annotation revealed predicted 4,454 protein coding open reading frames (ORFs), 43 tRNAs, 5 rRNAs; 2,793 (or 62%) the ORFs had functional assignment. compared published R. 1 tropical...
The nuage is a germline-specific perinuclear structure that remains functionally elusive. Recently, the in Drosophila was shown to contain two of three PIWI proteins - Aubergine and Argonaute 3 (AGO3) are essential for germline development. bind PIWI-interacting RNAs (piRNAs) function epigenetic regulation transposon control. Here, we report novel component, PAPI (Partner PIWIs), contains TUDOR domain interacts with all via symmetrically dimethylated arginine residues their N-terminal...
Plants evolved an array of disease resistance genes (R genes) to fight pathogens. In the absence pathogen infection, NBS-LRR genes, which comprise a major subfamily R are suppressed by small RNA cascade involving microRNAs (miRNAs) that trigger biogenesis phased siRNAs (phasiRNAs) from gene transcripts. However, whether or how influence is unknown. this study, we isolate mutant with global defects in miRNAs and phasiRNAs Arabidopsis thaliana trace over accumulation nuclear localization...
GB virus C (GBV-C) is prevalent globally and particularly among individuals at risk of parental exposures. Based on genetic diversity, this now classified into six genotypes many subtypes with distinct geographical distribution. In study, 120 Injecting Drug Users (IDUs) were recruited from Yunnan province, China. Among them, 43 (35.8%) positive for GBV-C RNA, 70 (58.3%) 103 (85.8%) sero-positive HIV-1 HCV respectively. This revealed 18.3% IDUs having GBV-C/HIV/HCV triple infection, which...
MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs involved in the post-transcriptional gene regulation and play critical role plant growth, development stress responses. Watermelon (Citrullus lanatus L.) is one important agricultural crops worldwide. However, watermelon miRNAs phasiRNAs their functions not well explored. Here we carried out computational experimental analysis phased interfering (phasiRNAs) by analyzing 14 RNA profiles from roots, leaves, androecium, petals,...
Panax notoginseng is a prominent traditional Chinese medicinal herb, yet its yield and quality are significantly constrained by continuous cropping obstacles, primarily stemming from soil-related issues. This study analyzed soils subjected to various degrees of P. cultivation, without planting, natural forest floor soil planting. The objective was investigate variations in microbial communities, physicochemical properties, enzyme activities across different conditions. Macro-genome...
Phased, secondary siRNAs (phasiRNAs) represent a class of small RNAs in plants generated via distinct biogenesis pathways, predominantly dependent on the activity 22-nt miRNAs. Most miRNAs are processed by DCL1 from miRNA precursors containing an asymmetric bulge, yielding 22/21-nt miRNA/miRNA* duplex. Here we show that miR1510, soybean capable triggering phasiRNA production numerous nucleotide-binding leucine-rich repeat (NB-LRRs), previously described as 21 nt its mature form, primarily...
Lariats are formed by excised introns, when the 5' splice site joins with branchpoint (BP) during splicing. Although lariat RNAs usually degraded RNA debranching enzyme 1, recent findings in animals detected many under physiological conditions. By contrast, features of BPs and to what extent accumulate naturally largely unexplored plants. Here, we analyzed 948 sequencing data sets document plant on a genome-wide scale. In total, identified 13,872, 5199, 29,582, 13,478 Arabidopsis (
Drought stress is a major obstacle to agricultural production. Tibetan wild barley with rich genetic diversity useful for drought-tolerant improvement of cereals. MicroRNAs (miRNAs) play critical roles in controlling gene expression response various environment perturbations plants. However, the genome-wide profiles miRNAs and their targets drought are largely unknown barley. In this study, polyethylene glycol (PEG) induced hydroponic experiment was performed, from roots two contrasting...
Abstract A family of DEDDh 3′→5′ exonucleases known as Small RNA Degrading Nucleases (SDNs) initiates the turnover ARGONAUTE1 (AGO1)-bound microRNAs in Arabidopsis by trimming their 3′ ends. Here, we report crystal structure SDN1 (residues 2-300) complex with a 9 nucleotide single-stranded substrate, revealing that domain forms rigid interactions N-terminal and binds 4 nucleotides from end via its catalytic pocket. Structural biochemical results suggest C-terminal adopts an Recognition Motif...
Abstract The use of chemical fertilizers and pesticides led to a decline in the quality yield Bupleurum chinense . aim this study was determine effects Trichoderma harzianum biofertilizer on growth, yield, radix bupleuri microbial responses. results showed that T. promoted growth B. increased bupleuri. In addition, it contents NH 4 + –N, NO 3 − available K, P activities sucrase catalase rhizosphere soil. High‐throughput analysis dominant bacteria were Proteobacteria (28%), Acidobacteria...
The tree shrew (Tupaia belangeri chinensis), a small animal widely distributed in Southeast Asia and southwest China, has the potential to be developed as an model for hepatitis C. To determine susceptibility of C virus (HCV) infection vitro vivo, well-established HCV, produced from J6/JFH1-Huh7.5.1 culture system, was used infect cultured primary tupaia hepatocytes (PTHs) shrews. results showed that HCV genomic RNA HCV-specific nonstructural protein 5A (NS5A) could detected PTH cell days...