A5009813094- Neuroscience and Neuropharmacology Research
- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Diet, Metabolism, and Disease
- Genetics and Neurodevelopmental Disorders
- Neuropeptides and Animal Physiology
- Ion channel regulation and function
- Memory and Neural Mechanisms
- Cannabis and Cannabinoid Research
- Neuroendocrine regulation and behavior
- Neurogenesis and neuroplasticity mechanisms
- Photochromic and Fluorescence Chemistry
- Receptor Mechanisms and Signaling
- Liver Disease Diagnosis and Treatment
- Autism Spectrum Disorder Research
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Photoreceptor and optogenetics research
- RNA regulation and disease
- Chromosomal and Genetic Variations
- PARP inhibition in cancer therapy
- Neural dynamics and brain function
- Birth, Development, and Health
- GABA and Rice Research
Biomedical Research and Innovation Institute of Cadiz
2019-2024
Universidad de Cádiz
2019-2024
Instituto de Salud Carlos III
2019-2024
Centro de Investigación Biomédica en Red de Salud Mental
2019-2024
Universidad Pablo de Olavide
2008-2021
Hospital Universitario Puerta del Mar
2020-2021
BackgroundPain affects both sensory and emotional aversive responses, often provoking anxiety-related diseases when chronic. However, the neural mechanisms underlying interactions between anxiety chronic pain remain unclear.MethodsWe characterized sensory, emotional, cognitive consequences of neuropathic (chronic constriction injury) in a rat model. Moreover, we determined role locus coeruleus (LC) neurons that project to basolateral amygdala (BLA) using DREADD (designer receptor exclusively...
Abstract There is strong comorbidity between chronic pain and depression, although the neural circuits mechanisms underlying this association remain unclear. By combining immunohistochemistry, tracing studies western blotting, with use of different DREADDS (designer receptor exclusively activated by designer drugs) behavioural approaches in a rat model neuropathic (chronic constriction injury), we explore how arises. To end, evaluated time-dependent plasticity noradrenergic locus coeruleus...
Memory formation requires changes in gene expression, which are regulated by the activation of transcription factors and epigenetic factors. Poly[ADP]-ribosylation nuclear proteins has been postulated as a chromatin modification involved memory consolidation, although mechanisms not well characterized. Here we demonstrate that poly[ADP]-ribose polymerase 1 (PARP-1) activity poly[ADP]-ribosylation over specific time course is required for synaptic plasticity related to stabilization mice. At...
Aging and fertility are two interconnected processes. From invertebrates to mammals, absence of the germline increases longevity. Here we show that loss function sul-2, Caenorhabditis elegans steroid sulfatase (STS), raises pool sulfated hormones, longevity ameliorates protein aggregation diseases. This increased requires factors involved in germline-mediated (daf-16, daf-12, kri-1, tcer-1 daf-36 genes) although sul-2 mutations do not affect fertility. Interestingly, is only expressed...
There is evidence that adult hippocampal neurogenesis influences function, although the role these neurons fulfill in learning and consolidation processes remains unclear. Using a novel fast X-ray ablation protocol to deplete neurogenic cells, we demonstrate immature are required for long-term memory formation. Moreover, found formation object recognition passive avoidance tests, two paradigms involve circuits with distinct emotional components, had different temporal demands on...
Aging, mental retardation, number of psychiatric and neurological disorders are all associated with learning memory impairments. As the underlying causes such conditions very heterogeneous, manipulations that can enhance in mice under different circumstances might be able to overcome cognitive deficits patients. The M-current regulates neuronal excitability action potential firing, suggesting its inhibition may increase capacities. We demonstrate XE991, a specific blocker, enhances healthy...
Abstract Adult hippocampal neurogenesis appears to influence functions, such as memory formation for example. While adult is known be involved in hippocampal-dependent learning and consolidation processes, the role of immature neurons reconsolidation, a process modification stored memories, remains unclear. Here, using novel fast X-ray ablation protocol deplete neurogenic cells, we have found that required update object recognition more than reinforce it. Indeed, show were selectively...
In addition to social and cultural factors, sex differences in the central nervous system have a critical influence on behavior, although neurobiology underlying these remains unclear. Interestingly, Locus Coeruleus (LC), noradrenergic nucleus that exhibits sexual dimorphism, integrates signals are related diverse activities, including emotions, cognition pain. Therefore, we set-out evaluate behaviors LC nucleus, subsequently, assess morphology function.Female male C57BL/6J mice were studied...
A problem of systemic pharmacotherapy is off-target activity, which causes adverse effects. Outstanding examples include neuroinhibitory medications like antiseizure drugs, are used against epilepsy and neuropathic pain but cause side There a need drugs that inhibit nerve signals locally on-demand without affecting other regions the body. Photopharmacology aims to address this with light-activated localized illumination in target organ. Here, we have developed photoswitchable derivatives...
Abstract Background One of the most unusual sources phylogenetically restricted genes is molecular domestication transposable elements into a host genome as functional genes. Although these kinds events are sometimes at core key macroevolutionary changes, their origin and organismal function generally poorly understood. Results Here, we identify several previously unreported element in human mouse genomes. Among them, find remarkable that gave rise to multigenic family placental mammals,...
In the last decade, new non-apoptotic roles have been ascribed to apoptotic caspases. This family of proteins plays an important role in sculpting brain early stages development by eliminating excessive and nonfunctional synapses extra cells. Consequently, impairments this process can underlie many neurological mental illnesses. view is particularly relevant dopamine because it a pleiotropic motor control, motivation, reward processing. study, we analyze effects elimination caspase-8 (CASP8)...
Background Dynamic changes in neuronal activity and noradrenergic locus coeruleus (LC) projections have been proposed during the transition from acute to chronic pain. Thus, authors explored cellular cFos of LC its conjunction with spontaneous pain-like behavior neuropathic rats. Methods Tyrosine hydroxylase:Cre wild-type Long–Evans rats, males females, were subjected constriction injury (CCI) for 2 (short-term, CCI-ST) or 30 days (long-term, CCI-LT), evaluating Fluoro-Gold expression LC,...
The transition from acute to chronic pain results in maladaptive brain remodeling, as characterized by sensorial hypersensitivity and the ensuing appearance of emotional disorders. Using constriction injury sciatic nerve a model neuropathic male Sprague-Dawley rats, we identified time-dependent plasticity locus coeruleus (LC) neurons related site injury, ipsilateral (LCipsi) or contralateral (LCcontra) lesion, hypothesizing that LC→dorsal reticular nucleus (DRt) pathway is involved...
Synaptic plasticity involves short- and long-term events, although the molecular mechanisms that underlie these processes are not fully understood. The transient A-type K+ current (IA) controls excitability of dendrites from CA1 pyramidal neurons by regulating back-propagation action potentials shaping synaptic input. Here, we have studied how decreases in IA affect cognitive plasticity. Using wild-type mice treated with 4-AP, an inhibitor, lacking DREAM protein, a transcriptional repressor...
The presented article describes data from secondary analyses, related to the research entitled "Cannabis consumption and Non-Alcoholic Fatty Liver Disease. A three years longitudinal study in first episode non-affective psychosis patients" [1]. We present detailed regarding socio-demographic baseline clinical characteristics of a sample 390 drug-naïve patients with psychosis, differences between cannabis users non-users those characteristics. Tables also show results cross-sectional...
Background and Purpose Cannabidiol (CBD) has garnered increasing interest for its potential use in the management of metabolic diseases. While nanoformulations offer a promising avenue to enhance CBD's oral bioavailability therapeutic efficacy, comprehensive studies on safe effective CBD-nanoformulations employing multi-target approach disorder treatment are lacking.Experimental approachWe engineered poly (lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) encapsulating CBD by...
Abstract A problem of systemic pharmacotherapy is off‐target activity, which causes adverse effects. Outstanding examples include neuroinhibitory medications like antiseizure drugs, are used against epilepsy and neuropathic pain but cause side There a need drugs that inhibit nerve signals locally on‐demand without affecting other regions the body. Photopharmacology aims to address this with light‐activated localized illumination in target organ. Here, we have developed photoswitchable...
Aging and fertility are two interconnected processes. From invertebrates to mammals, absence of the germline increases longevity by a still not fully understood mechanism. We find that loss function sul-2, Caenorhabditis elegans steroid sulfatase (STS), raises pool sulfated hormones longevity. This increased requires factors involved in germline-mediated (daf-16, daf-12, kri-1, tcer-1 daf-36 genes) is additive germline-less mutants. Noteworthy, sul-2 mutations do affect fertility. Thus, STS...