A5058922838- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Genetic Associations and Epidemiology
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Insect Resistance and Genetics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Neurobiology and Insect Physiology Research
- Bacterial Genetics and Biotechnology
- Nutrition, Genetics, and Disease
- Smoking Behavior and Cessation
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical Synthesis and Analysis
- Bioinformatics and Genomic Networks
- Health, Environment, Cognitive Aging
- Fatty Acid Research and Health
- Modular Robots and Swarm Intelligence
- Insect and Pesticide Research
- thermodynamics and calorimetric analyses
- Eicosanoids and Hypertension Pharmacology
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Innovation and Socioeconomic Development
University of Chicago
2015-2024
University of North Carolina at Chapel Hill
2014-2021
University of Michigan
2017
Public Health Department
2017
Florida State University
2012-2016
Institute of Molecular Biology and Biophysics
2015
Zhejiang University
2006-2008
Bacterial and archaeal clustered regularly interspaced short palindromic repeat (CRISPR) loci capture virus plasmid sequences use them to recognize eliminate these invaders. CRISPR RNAs (crRNAs) containing the acquired are incorporated into effector complexes that destroy matching invader nucleic acids. The multicomponent Cmr complex cleaves RNA targets complementary crRNAs. Here, we report cryoelectron microscopy reconstruction of a functional bound with target at ∼12 Å. Pairs Cmr4 Cmr5...
Abstract Background Nicotinic acetylcholine receptors (nAChRs) mediate fast synaptic cholinergic transmission in the insect central nervous system. The nAChR is molecular target of a class insecticides, neonicotinoids. Like mammalian nAChRs, nAChRs are considered to be made up five subunits, coded by homologous genes belonging same family. subunit Drosophila melanogaster , Apis mellifera and Anopheles gambiae have been cloned previously based on their genome sequences. silkworm Bombyx mori...
The Cmr complex is the multisubunit effector of type III-B clustered regularly interspaced short palindromic repeats (CRISPR)-Cas immune system. recognizes a target RNA through base pairing with integral CRISPR (crRNA) and cleaves at multiple spaced locations within complementary region. To understand molecular basis function this complex, we have assembled information from electron microscopic X-ray crystallographic structural studies mutagenesis complete Pyrococcus furiosus complex. Our...
Abstract The DIR2s RNA aptamer, a second-generation, in-vitro selected binder to dimethylindole red (DIR), activates the fluorescence of cyanine dyes, DIR and oxazole thiazole blue (OTB), allowing detection two well-resolved emission colors. Using Fab BL3-6 its cognate hairpin as crystallization module, we solved crystal structures both apo OTB-SO 3 bound forms at 2.0 Å 1.8 resolution, respectively. adopts compact, tuning fork-like architecture comprised helix short stem-loops oriented in...
Though knotting and entanglement have been observed in DNA proteins, their existence RNA remains an enigma. Synthetic topological structures are significant for understanding the physical biological properties pertaining to topology, these turn could facilitate identifying naturally occurring topologically nontrivial molecules. Here we show that containing single-stranded (ssRNA) free of strong base pairing interactions can be created either by configuring RNA-DNA hybrid four-way junctions...
Abstract Complementary DNAs encoding two types of acetylcholinesterase (AChE) were isolated from the silkworm, Bombyx mori. The type 1 ( Bmace1 ) and 2 Bmace2 ORFs are 2052 1917 bp in length, respectively. Both complete Bmaces C‐terminal truncated forms recombined into Bacmid baculovirus vector under control polyhedrin promoter expressed Trichoplusia ni (Tn‐5B1‐4) cells. resulting products exhibited AChE activity glycosylation proteins. An inhibition assay indicated that ace2‐type enzyme was...
The repeat-associated mysterious proteins (RAMPs) comprise the most abundant family of involved in prokaryotic immunity against invading genetic elements conferred by clustered regularly interspaced short palindromic repeat (CRISPR) system. Cas6 is one first characterized RAMP and a key enzyme required for CRISPR RNA maturation. Despite strong structural homology with other that bind hairpin RNA, distinctly recognizes single-stranded RNA. Previous biochemical studies show captures 5' end...
Abstract Picornaviral IRES elements are essential for initiating the cap-independent viral translation. However, three-dimensional structures of these remain elusive. Here, we report a 2.84-Å resolution crystal structure hepatitis A virus domain V (dV) in complex with synthetic antibody fragment—a crystallization chaperone. The RNA adopts three-way junction structure, topologically organized by an adenine-rich stem-loop motif. Despite no obvious sequence homology, dV architecture shows...
Abstract As one of its goals, synthetic biology seeks to increase the number building blocks in nucleic acids. While efforts towards this goal are well advanced for DNA, they have hardly begun RNA. Herein, we present a crystal structure an RNA riboswitch where stem C:G pair has been replaced by between two components artificially expanded genetic‐information system (AEGIS), Z and P , (6‐amino‐5‐nitro‐2(1 H )‐pyridone 2‐amino‐imidazo[1,2‐a]‐1,3,5‐triazin‐4‐(8 )‐one). The shows that : does not...