Animal-derived Proteus mirabilis (P. mirabilis) is an important food-borne zoonotic bacillus and widely exists in the broiler-breeding industry. The present study was designed to explore P. prevalence antimicrobial resistance characteristics 6 conventional broiler-fattening farms Shandong Province, China. overall isolation rate of 7.07% (50/707). Antimicrobial very common isolated from these varied for different antibacterial drugs, with chloramphenicol, ciprofloxacin,...

Klebsiella pneumoniae (K. pneumoniae) is a conditionally pathogenic bacterium present in the intestinal or respiratory tract of animals, and it common factor acquired infections major threat to public health. Increased production extended-spectrum β-lactamases (ESBLs) has become serious issue treatment K. infections. In this study, we examined serotypes antibiotic resistance profiles isolated from broiler chickens on farms Shandong Province, China. The isolation rate was 4.67% (33/707),...

Pseudorabies virus (PRV) infects numerous species of domestic and wild animals leading to severe diseases especially in swine cattle. Since 2011, the variant PRVs were identified pigs, which genetically different from classic strains. Although PRV infection is widely observed there still no report Here, we reported a natural acute bovine death Eastern China. Our study suggests that new strains could be potential threat cattle industry possibly public health human.

Abstract Background Porcine circovirus type 2 (PCV2) is one of the crucial swine viral pathogens, caused porcine associated diseases (PCVAD). Shandong province most important pork producing areas and bears a considerable economic loss due to PCVAD. However, there limited information on epidemiology coinfection rate PCV2 with other critical in this area, such as reproductive respiratory syndrome virus (PRRSV), classical fever (CSFV), Pseudorabies (PRV), epidemic diarrhea (PEDV). Results...

Colibacillosis, caused by Escherichia coli, is one of the most common bacterial diseases chickens. The high incidence and considerable economic losses associated with colibacillosis make it a significant concern worldwide. In recent years, efficacy colistin has been severely impacted emergence plasmid-mediated resistance genes, especially mcr-1. Therefore, monitoring antibiotic resistance, particularly amongst E. coli strains vitally important to future growth sustainability poultry...

Introduction Brucellosis, an infectious zoonotic disease caused by members of the genus Brucella , results in chronic multi-organ injury. Improving specificity and sensitivity serological methods for diagnosing brucellosis necessitates development novel diagnostic antigens. The twin-arginine translocation (Tat) pathway is responsible transporting folded proteins across cytoplasmic membrane has been implicated virulence . Three Tat substrate proteins—L,D-transpeptidase ErfK (A0577), linear...

Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic infections in humans and animals. Mycobacterium abscessus (Mab) is a rapidly growing known for its resistance to multiple antibiotics ability respiratory, skin, mucosal infections. Understanding the distribution prevalence of NTM, particularly Mab, cattle farms slaughterhouses crucial developing effective prevention control measures. We collected swabs from various surfaces (e.g., feed troughs, sinks,...

Background The influenza viruses circulating in animals sporadically transmit to humans and pose pandemic threats. Animal models evaluate the potential public health risk of these are needed. Methodology/Principal Findings We investigated guinea pig as a mammalian model for study replication transmission characteristics selected swine H1N1, H1N2, H3N2 avian H9N2 viruses, compared those (H1N1) 2009 seasonal human viruses. were restricted respiratory system pigs shed at high titers nasal...

In recent years, the poultry industry had been markedly affected by adenoviral diseases such as hydropericardium syndrome and inclusion body hepatitis caused fowl adenovirus (FAdV), which have become increasingly prevalent in China. Shandong Province, China, is an important area for breeding where various complex diverse FAdV serotypes were isolated. However, dominant strains their pathogenic characteristics are not yet reported. Therefore, a pathogenicity epidemiological survey of was...

Pseudorabies, also known as Aujeszky's disease, is caused by the pseudorabies virus (PRV) and has been recognized a critical disease affecting pig industry wide range of animals around world, resulting in great economic losses each year. Shandong province, one most vital food animal-breeding regions China, very dense population, within which infections were detected recent years. The data, however, on PRV epidemiology coinfection rates with other major swine diseases sparse.This study aimed...

The extensive use of antibiotics has, in recent years, caused antimicrobial resistance and multidrug Escherichia coli to gradually develop into a worldwide problem. These resistant E. could be transmitted humans through animal products feces the environment, thereby creating problem for bacterial treatment animals resulting public health issue. Monitoring throughout broiler fattening period is therefore great significance both poultry industry health. In this longitudinal study, samples were...

Since the rise of porcine reproductive and respiratory syndrome virus (PRRSV) in China, mutations have occurred regularly. In particular, emergence HP-PRRSV has significantly improved pathogenicity PRRSV. It brought huge economic losses to Chinese pig farming industry. To understand current prevalence evolution PRRSV Shandong Province, 1,344 samples suspected having were collected from local hog farms different sizes. Genetic variation isolated ORF5 gene was analyzed using RT-PCR method. The...

Pseudorabies (PR) is a highly contagious disease affecting wide range of animals, which annually causes great economic losses in China. In this study, total number 18,815 serum samples and 1,589 tissue were collected from 311 intensive pig farms (≥350 sows) located eight cities Heilongjiang province, tested by ELISA PCR. Overall, the positive rates gE gB protein 16.3% 84.5%, respectively. The PCR-positive rate PR virus (PRV) was 17.8%. coinfection PRV with porcine reproductive respiratory...

A Newcastle disease virus (NDV) isolated from an outbreak in racing pigeons China was characterized this study. Complete gene of the NDV isolate sequenced and phylogenetic analysis. Pathogenicity experiment carried out pigeons, chickens, ducks. Phylogenetic analysis revealed that strain clustered with Class II viruses, has highly phylogenetically similar to strains China, but distant viruses prevalence chickens vaccine used China. The deduced amino acid sequence cleavage site fusion (F)...

Paratuberculosis, a chronic and sometimes fatal disease of ruminants, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, we examined cases among 2–4-year-old dairy cows at farms in Shandong Province, China. Paratuberculosis were diagnosed based on clinical symptoms, pathological autopsy, histopathological inspection. Characteristics the affected cattle included poor body condition, persistent diarrhea, subcutaneous edema, granulomatous ileitis (multibacillary),...

HoBi-like pestivirus is an emerging atypical in cattle and small ruminants, causing clinical signs similar to those observed bovine viral diarrhoea virus infections. Natural infection of has been reported herds ruminants multiple countries South America, Europe Asia. However, pestiviruses were only identified from contaminated serum China. So far, no cases induced by Chinese herds. Here, for the first time, we natural a herd Sick with severe respiratory high fatality rate found beef Shandong...

Enterococcus faecalis (E. faecalis) is a zoonotic pathogen that causes severe economic losses in the poultry-breeding industry. In our study, cecal samples from broilers with enlargement at slaughterhouses Tai'an, China, were analyzed. The results revealed 61 E. strains had drug resistance rates ranging 96.72 to 8.20% against 11 antibiotics 5 classes, of which erythromycin (96.72%) and tetracycline highest vancomycin (8.20%) lowest. detection rate multiple drug-resistant isolates was 72.13%....

In a retrospective study, the antimicrobial susceptibility of Enterococcus spp. isolated from stored sedimentation dust samples cattle, pig and poultry barns to 16 antibiotics was determined using microdilution test. The resistance phenotypes 70 isolates different timespans (8 1980s, 15 1990s, 43 2000s, 4 2015) were determined. Resistant enterococci detected in all time periods. Resistances three or more occurred 69 percent isolates. oldest multidrug resistant isolate an faecium obtained...

H9N2 avian influenza has been prevalent in chicken flocks of China for years. In the first half year 2018, clinical cases suspected infection were collected from Shandong province. Nine strains virus isolated. The pathological changes dead chickens mainly respiratory inflammation, renal swelling, and secondary infection. microscopic lesions consistent with pathogenic characteristics virus. From November 2017 to June a total 3,380 serum samples randomly commercial laying hens Province. H9...

Brucellosis is one of the most important zoonotic diseases in world. Canine brucellosis, caused mainly by Brucella canis, seriously neglected, and there a lack accurate diagnostic tools.In this study, to compare BP26, Omp25, Omp31 multiepitope-based fusion protein serological detection canine using 34 brucellosis-positive dog sera 62 negative control sera, outer membrane proteins Omp31, Omp25 were evaluated iELISA for their potential use as antigens diagnosis brucellosis.The results showed...

Responsible for the acute infectious disease porcine epidemic diarrhea (PED), PED virus (PEDV) induces severe and high mortality in infected piglets thus severely harms productivity economic efficiency of pig farms. In our study, we aimed to investigate analyze recent status incidence pattern PEDV infection some areas Shandong Province, China. We collected 176 clinical samples from farms different regions Province during 2019-2021. PEDV, TGEV, PORV were detected using RT-PCR. The full-length...

Brucella BP26 proves to be a highly immunogenic antigen with excellent specificity in brucellosis detection. In China, the authorized use of Bp26-deleted vaccine M5ΔBP26 for preventing small ruminant highlights importance developing accurate detection methods targeting BP26, particularly diagnosis differentiation between infected and vaccinated animals (DIVA). Using traditional mouse hybridoma technique, we successfully obtained 12 monoclonal antibodies (mAbs) BP26. The efficacy these mAbs...