A5101489942- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac Ischemia and Reperfusion
- Neuroscience and Neuropharmacology Research
- Anesthesia and Neurotoxicity Research
- Neurological Disease Mechanisms and Treatments
- Thermal Regulation in Medicine
- Immune cells in cancer
- Extracellular vesicles in disease
- Acute Ischemic Stroke Management
- MicroRNA in disease regulation
- Cardiac Arrest and Resuscitation
- Autophagy in Disease and Therapy
- Cancer-related molecular mechanisms research
- Neonatal and fetal brain pathology
- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Biochemical effects in animals
- PI3K/AKT/mTOR signaling in cancer
- Traumatic Brain Injury and Neurovascular Disturbances
- Circular RNAs in diseases
- RNA regulation and disease
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
- Lipid metabolism and disorders
Capital Medical University
2014-2025
Jiaxing University
2024
Stanford University
2012-2021
Beijing Luhe Hospital Affiliated to Capital Medical University
2017-2018
Palo Alto University
2017
Jinan University
2017
Henan University Huaihe Hospital and Huaihe Clinical Institute
2015
Huashan Hospital
2014
Capital University
2014
Fudan University
2014
Cerebral ischemic preconditioning protects against stroke, but is clinically feasible only when the occurrence of stroke predictable. Reperfusion plays a critical role in cerebral injury after stroke; we tested hypothesis that interrupting reperfusion lessens injury. We found for first time such postconditioning with series mechanical interruptions significantly reduces damage. Focal ischemia was generated by permanent distal middle artery (MCA) occlusion plus transient bilateral common...
In order to promote preclinical and clinical applications of photoacoustic imaging, novel contrast agents are highly desired for molecular imaging diseases, especially deep tumor imaging. Here, perylene-3,4,9,10-tetracarboxylic diiimide-based near-infrared-absorptive organic nanoparticles reported as an efficient agent brain tumors in living mice with enhanced permeability retention effect.
The aims of this study were to examine the alternation in serum exosome concentrations and levels exosomal miR-9 miR-124, two brain-specific miRNAs, acute ischemic stroke (AIS) patients explore predictive values these miRNAs for AIS diagnosis damage evaluation. Sixty-five with at stage enrolled 66 non-stroke volunteers served as controls. Serum exosomes isolated by ExoQuick precipitations characterized transmission electron microscopy, nanoparticle-tracking analysis western blotting. miR-124...
Abstract Bcl‐2 protects against both apoptotic and necrotic death induced by several cerebral insults. We others have previously demonstrated that defective herpes simplex virus vectors expressing protect various insults in vitro vivo , including ischemia. Because the infarct margin may be a region is most amenable to treatment, we first determined whether gene transfer possible using focal ischemia model. Since ischemic injury with without reperfusion occur different mechanisms, also either...
Abstract We previously reported that ischemic postconditioning with a series of mechanical interruptions reperfusion reduced infarct volume 2 days after focal ischemia in rats. Here, we extend this data by examining long‐term protection and exploring underlying mechanisms involving the Akt, mitogen‐activated protein kinase (MAPK) C (PKC) signaling pathways. Post‐conditioning improved behavioral function assessed 30 stroke. Additionally, increased levels phosphorylated Akt (Ser473) as...
Rationale: Peripheral blood monocytes are recruited into the ischemic brain and transform macrophages after stroke.Nevertheless, exact role of CCR2-dependent monocytes/ in injury stroke remains elusive.Methods: We used CCR2 knockout (KO) mice pharmacological inhibitor, propagermanium (PG), to address monocytes/macrophages acute stage neurological functional recovery middle cerebral artery (MCA) occlusion reperfusion.Results: KO resulted smaller infarct size lower mortality than wild type...
The microglia cell transfer has been shown to play a protective role in ischemic stroke. Microglia cells may this nerve-protective via the promotion of angiogenesis. However, underlying mechanisms are largely unknown and need further investigation.The aim study was investigate pro-angiogenesis effects unpolarized, interleukin-4 (IL-4)-polarized or lipopolysaccharide (LPS)-polarized BV2 both vivo vitro. We also investigated potential these effects.BV2 were polarized using phosphate-buffered...
Abstract We investigated whether HSV gene transfer of HSP72 in vivo and vitro: (1) protected cornu ammonis 1 region the hippocampus neurons from global cerebral ischemia; (2) affected Bcl‐2 expression. vectors expressing β‐galactosidase (reporter) or only (control vector) were injected into 15 hours before induction ischemia (n = 10) sham‐operated rats 8). vector–treated displayed significantly more surviving transfected (X‐gal‐positive, 31 ± 8) compared with control (10 4) after ischemia....
We and others have reported that rapid ischemic postconditioning, interrupting early reperfusion after stroke, reduces infarction in rats. However, its extremely short therapeutic time windows, from a few seconds to minutes reperfusion, may hinder clinical translation. Thus, this study we explored if delayed which is conducted hours offers protection against stroke.Focal ischemia was generated by 30 min occlusion of bilateral common carotid artery (CCA) combined with permanent middle...
T cells and their subsets modulate ischemic brain injury. We studied the effects of absence cell on infarction after in vivo stroke then used an vitro coculture system splenocytes neurons to further identify roles neuronal death.Stroke was induced by middle cerebral artery suture occlusion mice infarct sizes were measured 2 days poststroke. Splenocytes cocultured with neurons, survival 3 later.A deficiency both B (severe combined immunodeficiency) paucity CD4 or CD8 equally resulted smaller...
We recently demonstrated that limb remote preconditioning (LRP) protects against focal ischemia measured 2 days post-stroke. Here, we studied whether LRP provides long-term protection and improves neurological function. also investigated transmits its protective signaling via the afferent nerve pathways from preconditioned to ischemic brain inflammatory factors are involved in LRP, including novel galectin-9/Tim-3 cell pathway, which induces death lymphocytes. left hind femoral artery was...
Circular RNAs (circRNAs) have been demonstrated to act as microRNA (miRNA) sponges and they play important roles in regulating gene expression through a circRNA-miRNA-gene pathway. The specific of circRNAs the pathogenesis cerebral ischemia, however, are still unclear. Thus, aim this study is determine circRNA profiles ischemic brain after stroke, which was induced by 45 min transient middle artery occlusion (MCAO). results from microarrays revealed that 1027 were significantly altered 48...
Protein kinases Akt1 and Akt3 are considered to be more crucial brain function than Akt2. We investigated the roles of in stroke-induced injury examined their interactions with Akt/mTOR pathways. Focal ischemia was induced rats. Lentiviral vectors expressing constitutively active (cAkt1 cAkt3) were injected into ischemic cortex. Infarct sizes gene protein expressions pathways evaluated. The results show that proteins degraded as early 1 hour after stroke, whereas Akt2 remained unchanged...
Background Limb remote ischemic preconditioning ( RIPC ) protects against brain injury induced by stroke, but the underlying protective mechanisms remain unknown. As hypoxia inducible factor 1α HIF ‐1α) is neuroprotective in stroke and mediates neuroinflammation, we tested hypothesis that ‐1α a key of mediating inflammation. Methods Results Stroke was transient middle cerebral artery occlusion rats, conducted both hind limbs. The mRNA examined quantitative reverse transcription polymerase...
Apoptosis plays a critical role in many neurologic diseases, including stroke. Cytochrome c release and activation of various caspases are known to occur after focal global ischemia. However, recent reports indicate that caspase-independent pathways may also be involved ischemic damage. Apoptosis-inducing factor (AIF) is novel flavoprotein helps mediate apoptotic cell death. AIF translocates from mitochondria nuclei where it induces DNA fragmentation. Bcl-2, mitochondrial membrane protein,...
Abstract We examined the temporal factors of postconditioning, assessed whether gradual reperfusion reduces infarcts, and compared postconditioning's protection with that both rapid delayed preconditioning. Focal ischemia was generated by permanent occlusion leftdistal middle cerebral artery (dMCA) combined 30 min common carotid arteries (CCA) in rats. Postconditioning performed repetitive brief release CCA after occlusion. Gradual controlled bilateral CCA. confirmed postconditioning...
While pre-conditioning is induced before stroke onset, ischemic post-conditioning (IPostC) performed after reperfusion, which typically refers to a series of mechanical interruption blood reperfusion stroke. IPostC known reduce infarction in wild-type animals. We investigated if protects against brain injury by focal ischemia Tcell-deficient nude rats and examine its effects on Akt the mammalian target rapamycin (mTOR) pathway. Although reduced infarct size at 2 days post-stroke rats, it did...