A5110677071- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Milk Quality and Mastitis in Dairy Cows
- Genetic Mapping and Diversity in Plants and Animals
- MicroRNA in disease regulation
- Genetic diversity and population structure
- Animal Genetics and Reproduction
- Meat and Animal Product Quality
- Circular RNAs in diseases
- Lipid metabolism and biosynthesis
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Metabolism, Diabetes, and Cancer
- Effects of Environmental Stressors on Livestock
- Fatty Acid Research and Health
- Infant Nutrition and Health
- Digestive system and related health
- Reproductive Physiology in Livestock
- Probiotics and Fermented Foods
- Ruminant Nutrition and Digestive Physiology
- NF-κB Signaling Pathways
- Insect and Pesticide Research
- Lipid metabolism and disorders
- Epigenetics and DNA Methylation
- Animal Nutrition and Physiology
Yangzhou University
2016-2025
Zhejiang A & F University
2025
Shenyang Medical College
2024
Westlake University
2024
Kerala Veterinary and Animal Sciences University
2021
University of Guelph
2018
Huazhong Agricultural University
2012-2015
Southwest Minzu University
2012
MicroRNAs are small non-coding RNA molecules that important regulators of gene expression at the post-transcriptional level. miRNAs impact processes cell proliferation, differentiation and apoptosis. Thus, regulation miRNA profiles associated with mastitis will be conducive for its control. In this study, Staphylococcus aureus (S. aureus) was administered to mammary gland Chinese Holstein cows construct a bacteria-type model. Total isolated from bovine tissue samples S. aureus-induced group...
The domestic water buffalo is native to the Asian continent but through historical migrations and recent importations, nowadays has a worldwide distribution. two types of buffalo, i.e. river swamp, display distinct morphological behavioural traits, different karyotypes also have purposes geographical distributions. River buffaloes from Pakistan, Iran, Turkey, Egypt, Romania, Bulgaria, Italy, Mozambique, Brazil Colombia, swamp China, Thailand, Philippines, Indonesia were genotyped with...
Research on the mechanisms that regulate milk fat synthesis in dairy cows is essential to identify potential molecular targets long term can help develop appropriate breeding programs. Although some studies have revealed microRNA (miRNA) affect lipid metabolism by targeting specific genes, joint analysis of miRNA and target mRNA data from bovine mammary tissue has few clues regarding underlying controlling synthesis. The objective present study was use high-throughput sequencing...
Milk contains a number of beneficial fatty acids including short and medium chain unsaturated conjugated nonconjugated acids. In this study, microRNA sequencing mammary tissue collected in early-, peak-, mid-, late-lactation periods was performed to determine the miRNA expression profiles. miR-16a one differentially expressed selected for in-depth functional studies pertaining acid metabolism. The mimic impaired fat metabolism [triacylglycerol (TAG) cholesterol] while knock-down promoted...
Cadmium is a common environmental heavy metal pollutant that can accumulate over long periods of time and cause disease. Thus, analysis the molecular mechanisms affected by cadmium in body could be great significance for prevention treatment cadmium-related diseases. In this study, flow cytometry, immunofluorescence, transmission electron microscopy, H&E (Hematoxylin Eosin) staining TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used to verify induced apoptosis immune responses...
Theileria spp. are tick transmitted protozoa that can infect large and small ruminants causing disease economic losses. Diagnosis of infections is often challenging, as parasites be difficult to detect identify microscopically serology unreliable. While there PCR assays which certain spp., no one has been designed all recognized species occur in will greatly simplify the laboratory diagnoses infections. Primers probes for a genus-specific pan-Theileria FRET-qPCR were selected by comparing...
Intramuscular fat (IMF) content and fatty acid (FA) composition vary significantly across beef cattle breeds, which play an important role in taste nutritional value. However, the molecular mechanisms underlying these phenotypic differences remain unknown. The present study compared meat quality traits between Yunling Chinese Simmental cattle. showed a lower IMF proportion of monounsaturated acids (MUFA), as well higher proportions saturated (SFA), polyunsaturated (PUFA), short-chain (sc-FA)...
The quality of milk is inseparable from its components, and fatty acid content a key factor affecting the milk. In this study, miRNA mRNA profiles bovine mammary gland tissue during dry period peak lactation were determined through high-throughput sequencing. total, 72 miRNA–mRNA regulatory pathways screened, including miR-128/PPARGC1A pathways. miR-128 can directly target PPARGC1A inhibit expression. addition, study also observed that there was binding site in sequence circular RNA...
Udder conformation traits are one of the most economic in dairy cows, greatly affecting animal health, milk production, and producer profitability industry. Genetic analysis udder structure scores have been developed Holstein cattle. In our research, we conducted a genome-wide association study for five traits, including anterior attachment (AUA), central suspensory ligament (CSL), posterior height (PUAH), width (PUAW), depth (UD), which fixed random model circulating probability unification...
Biogenic volatile organic compounds (BVOCs) significantly impact air quality and climate. Mechanical injury is a common stressor affecting plants in both natural urban environments, it has potentially large influences on BVOC emissions. However, the interspecific variability wounding-induced emissions remains poorly understood, particularly for subtropical trees shrubs. In this study, we investigated effects of controlled mechanical isoprenoid aromatic compound taxonomically diverse set 45...
Holstein and Jersey cows, as excellent dairy breeds, have their own advantages in milk yield, quality, disease resistance, heat resistance. However, the adaptability rumen microbiome changes cows under stress are not clear. Therefore, main objective of this study was to compare differences tolerance ruminal stress. The experiment comprised a 7-day thermo-neutral (TN) period (HS) period. Five five with similar parity days were selected, fluid collected from them each. Compared TN period,...
Heat stress poses a significant challenge to dairy cattle, leading adverse physiological effects, reduced milk yield, impaired reproduction performance and economic losses. This study investigates the role of rumen microbiome in mediating heat resistance cows. Using entropy-weighted TOPSIS method, we classified 120 cows into heat-resistant (HR) heat-sensitive (HS) groups based on biochemical markers, including rectal temperature (RT), respiratory rate (RR), salivation index (SI) serum levels...
This study aimed to describe the expression profiles of microRNAs (miRNAs) from mammary gland tissues collected dairy cows with Streptococcus agalactiae-induced mastitis and identify differentially expressed miRNAs related mastitis. The glands Chinese Holstein were challenged agalactiae induce Small RNAs isolated test control groups then sequenced using Solexa sequencing technology construct two small RNA libraries. Potential target genes these predicted RNAhybrid software, KEGG pathways...
The composition and metabolic profile of the ruminal microbiome have an impact on milk composition. To unravel metabolome affecting fat synthesis in dairy cows, 16S rRNA internal transcribed spacer (ITS) gene sequencing, as well ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) methods were used to investigate significant differences bacterial fungal communities among Chinese Holstein cows with contrasting contents under same diet (H-MF 5.82 ± 0.41% vs. L-MF 3.60...
Mastitis is a highly prevalent disease in dairy cows that causes large economic losses. Streptococcus agalactiae common contagious pathogen and major cause of bovine mastitis. The immune response to intramammary infection with S. very complex biological process. To understand the host agalactiae-induced mastitis, mammary gland lactating Chinese Holstein was challenged via nipple tube perfusion. Visual inspection, analysis milk somatic cell counts, histopathology, transmission electron...
Anaplasma spp. and Ehrlichia are tick-transmitted bacteria that of significant economic importance as they can infect large small ruminants also people. There is little information on anaplasmosis ehrlichiosis in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic 12 provinces China for Positive further analyzed with a standard PCR the gltA . DNA was detected sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), water...
Abstract Dairy cow mastitis is a detrimental factor in milk quality and food safety. Mastitis generally refers to inflammation caused by infection pathogenic microorganisms. Our studies recent years have revealed the role of miRNA regulation Staphylococcus aureus ‐induced mastitis. In present study, we overexpressed suppressed miR‐145 investigate function Mac‐T cells. Flow cytometry, ELISA EdU staining were used detect changes secretion several cytokines cell proliferation. We found that...
Staphylococcus aureus intramammary infection is one of the most common causes chronic mastitis in dairy cows, whose development may be associated with epigenetic changes expression important host defense genes. This study aimed to construct a genome-wide DNA methylation profile mammary gland Chinese Holstein cows (n=3) following experimentally induced S. mastitis, and explore potential gene regulatory mechanisms affected by during mastitis. was extracted from aureus-positive aureus-negative...
Abstract As the main component of Gram-negative bacterial cell wall, lipopolysaccharide (LPS) is well documented as an inducer inflammation in bovine mammary cells. Lycium barbarum (goji) polysaccharides (LBP) have been used nonruminants prebiotics to improve growth performance, immune ability, and antioxidant capacity. We aimed investigate underlying effects LBPs on proinflammatory responses LPS-stimulated primary epithelial cells (bMECs). Cells were isolated from tissue three lactating...
Staphylococcus aureus- induced mastitis is one of the most intractable problems for dairy industry, which causes loss milk yield and early slaughter cows worldwide. Few studies have used a comprehensive approach based on integrative analysis miRNA mRNA expression profiles to explore molecular mechanism in bovine caused by S. aureus. In this study, aureus (A1, B1 C1) sterile phosphate buffered saline (PBS) (A2, B2 C2) were introduced different udder quarters three individual cows,...
The objective of this research was to explore the effect metformin on lipoteichoic acid (LTA)–induced mastitis model using isolated primary bovine mammary epithelial cells (PBMECs). PBMECs were exposed either 3 mM for 12 h as a group (MET) or 100 μg/mL LTA 6 (LTA). Cells pretreated with followed by washing and exposure served MET + group. Phosphate-buffered saline added control different doses analyzed flow cytometry (annexin V–fluorescein isothiocyanate assay) detect cell apoptotic rate. We...