A5075236759- Lung Cancer Treatments and Mutations
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Ubiquitin and proteasome pathways
- Metabolism, Diabetes, and Cancer
- Lung Cancer Diagnosis and Treatment
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Erythrocyte Function and Pathophysiology
- Cytokine Signaling Pathways and Interactions
- Cancer Cells and Metastasis
- Adipose Tissue and Metabolism
- Melanoma and MAPK Pathways
- Erythropoietin and Anemia Treatment
- Bone health and treatments
- Heme Oxygenase-1 and Carbon Monoxide
- NF-κB Signaling Pathways
Sichuan University
2015-2025
National Medical Products Administration
2023-2024
State Key Laboratory of Biotherapy
2012-2022
West China Hospital of Sichuan University
2019
Sichuan Cancer Hospital
2019
Tumor aerobic glycolysis, or the Warburg effect, plays important roles in tumor survival, growth, and metastasis. Pyruvate kinase isoenzyme M2 (PKM2) is a key enzyme that regulates glycolysis cells. Recent research has shown PKM2 can be used as marker for diagnosis and, particular, potential target cancer therapy. We investigated effects of combining shRNA targeting docetaxel on human A549 lung carcinoma cells both vivo vitro. observed significantly downregulate expression level PKM2. The...
Abstract Background Natural killer (NK) cells can kill tumor in a non-MHC-restricted manner. However, cancer frequently escape from the attack of NK by multiple ways. In this study, we investigated effect gefitinib on interaction between and lung cells. Methods 51 Cr release assay, CD107a IFN-γ secretion assay were performed to detect sensitivity cell lines A549 H1975 cytotoxicity presence gefitinib. Human co-cultured with NKG2D ligands, ULBP1, ULBP2, MICA, MHC-I cells, NKG2D, NKp44 NKp46...
Abstract Cancer-associated fibroblasts (CAFs) are common components of the tumor-suppressive microenvironment and a major determinant poor outcome therapeutic vaccination. In this study, we modified tumor cells to express fibroblast activation protein (FAP), which is highly expressed by CAFs, potentially improve whole-cell vaccines targeting both CAFs. Tumor were transfected with murine FAP plasmids bearing cationic lipid DOTAP. Its antitumor effects investigated in three established models....
SUMO-activating enzyme subunit 2 (SAE2) is the sole E1-activating required for numerous important protein SUMOylation, abnormal of which associated with carcinogenesis. SAE2 inactivation was recently reported to be a therapeutic strategy in cancers Myc overexpression. However, roles small cell lung cancer (SCLC) are largely unknown. Stably knockdown H446 cells were established lentiviral system. Cell viability, cycle, and apoptosis analyzed using MTT assay flow cytometric assay. Expression...
Neuropsychological factors have been shown to influence tumor progression and therapeutic response. The present study investigated the effect of dopamine receptor antagonist thioridazine on murine breast cancer. anti‑tumor efficacy was assessed using a cancer model. Cell apoptosis proliferation were analyzed in vitro flow cytometry (FCM) MTT assay, respectively. Western blot analysis performed assess Akt, phosphorylated (p)‑Akt, signal transducer activator transcription (STAT) 3, p‑STAT3...
Pleural and peritoneal metastasis accompanied by malignant pleural effusion (MPE) or ascites (MA) is frequent in patients with advanced solid tumors that originate from the lung, breast, gastrointestinal tract ovary. Regional delivery of CAR-T cells represents a new strategy to control tumor dissemination serous cavities. However, effusions constitute an immune-suppressive environment potentially induces cell dysfunction. Here, we demonstrated anti-tumor cytotoxicity conventional...
Abstract First-generation epidermal growth factor receptor (EGFR) targeted kinase inhibitors (TKIs) are still used in selected non-small cell lung cancer (NSCLC) patients despite the resistance. Based on correlation of programmed death ligand 1 (PD-L1) and EGFR signaling pathway, whether continuous TKIs treatment will affect PD-L1 expression after disease progression remains unclear. To investigate potential change TKI-resistant NSCLC treatment, we treated H1975 HCC827 for more than one...
Abstract The simultaneous increases in blood lactic acid and erythrocytes after intense exercise could suggest a link between lactate the erythropoiesis. However, effects of on erythropoiesis remain to be elucidated. Here, we utilized mouse model determine role this process parallel with studies using leukaemic K562 cells. Treatment cells vitro increased mRNA protein expression haemoglobin genes frequency GPA + Also, haematocrit CD71 − /Ter119 erythroid were observed acid-treated mice, which...
Abstract Hemorrhage is a common clinical manifestation in patients with cancer. Intratumor hemorrhage has been demonstrated to be poor prognostic factor for cancer patients. In this study, we investigated the role of RBCs and hemoglobin (Hb) process tumor progression therapeutical response. Hb potently promoted cell proliferation syngenic growth. activated reactive oxygen species–NF-κB pathway both cells macrophages. also induced chemoresistance mediated, part, by upregulating ABCB1 gene...
Malignant pleural effusion (PE) and ascites, common clinical manifestations in advanced cancer patients, are associated with a poor prognosis. However, the biological characteristics of malignant PE ascites not clarified. Here we report that can induce frequent epithelial-mesenchymal transition program endow tumor cells stem cell properties high efficiency, which promotes growth, chemoresistance, immune evasion. We determine this process is mainly dependent on VEGF, one initiator...
Discrimination of multiple non-small cell lung cancers (NSCLCs) as primary (MPLCs) or intrapulmonary metastases (IPMs) is critical but remains challenging. The aim this study to develop and validate the machine learning (ML) models based on molecular features for estimating probability MPLC IPM patients presenting NSCLCs. A total 72 NSCLCs with 157 surgical resection tumor lesions from January 2012 2018 at two institutions were included developing testing models. Specifically, 46 103 tumors...
Major histocompatibility complex (MHC) class I molecules have a crucial role in tumor immune evasion; however, the association of MHC with outcomes cancer patients remains controversial. Nucleotide-binding oligomerization-like receptor family caspase recruitment domain-containing 5 (NLRC5) has been reported to be transactivator. However, expression and function NLRC5 elucidated. The present study aimed retrospectively examine human tissues its clinical non-small-cell lung (NSCLC) stage III...
Epidermal growth factor receptor‑tyrosine kinase inhibitors (EGFR‑TKIs) have shown promising effects against the of non‑small cell lung cancer (NSCLC) cells harboring EGFR mutations (EGFR‑mts). However, many patients with NSCLC that are accepted for EGFR‑TKI treatment followed by chemotherapy possess an unknown status including wild‑type (EGFR‑wt). Little is known about potential prior to chemotherapy. We investigated and underlying molecular events 4 weeks continuous exposure EGFR‑TKIs in...
An increasing number of studies support the use metformin, a common antidiabetic drug, as novel anticancer therapeutic. However, its mechanism action has yet to be identified. In current study, metformin was observed effectively inhibit growth K‑ras mutant but not wild‑type tumors in vivo. The antitumor effects were mediated by induction apoptosis and inhibition proliferation addition, induced tumors, A549 PANC‑1, tumor, A431, vitro. Similarly, at lower concentrations, inhibited cell mutant,...
Adjuvants are important components of recombinant protein vaccines which often poorly immunogenic. For decades, the search for new vaccine adjuvants has been predominantly empirical. In addition, combinations more than one adjuvant plus antigen have systematically studied. Plasmid DNA containing additional oligodeoxynucleotides with unmethylated CpG motifs (CpG ODN) entrapped in liposomes used as an and shown powerful immunostimulatory functions. our study, combination plasmid 16 ODNs...
Breast cancer is the most commonly diagnosed type and leading cause of cancer-related deaths among women worldwide. Previous studies have reported contradictory performance chemokine CXC motif ligand 13 (CXCL13) in breast cancer. In this study, The Cancer Genome Atlas database analysis revealed that CXCL13 was overexpressed various human cancers including carcinoma, associated with good clinical prognosis Flow cytometry detection also found upregulated intracellular expression cell lines. To...
Curcumin has been proven to effectively inhibit tumor growth by both targeting cells and angiogenesis; however, poor water solubility limits further clinical application. In the present study, we prepared water-soluble liposomal curcumin investigate its anti-tumor effects underlying mechanism. The MTT assay was used test anti-proliferative activities for MS1 murine endothelial LL/2 Lewis lung cancer cell lines. Apoptosis cycle arrest induced were analysed flow cytometry. Anti-angiogenic...
Adeno-associated virus (AAV) is an ideal choice for gene delivery; however, its further development has been limited owing to low transduction efficiency. DNA-damaging agents can improve AAV-mediated transgene expression. Hyperthermia, as one of the oldest documented tumor treatment modalities, cause DNA damage well. However, combined consisting hyperthermia and therapy not reported yet. In this work we investigated whether pigment epithelium-derived factor (PEDF) delivery with synergistic...