A5102422034- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Antibiotic Resistance in Bacteria
- Diagnosis and treatment of tuberculosis
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- CRISPR and Genetic Engineering
- Syphilis Diagnosis and Treatment
- Cancer therapeutics and mechanisms
- Biochemical and Molecular Research
- Sugarcane Cultivation and Processing
- Dental Health and Care Utilization
- Advanced Biosensing Techniques and Applications
- Growth and nutrition in plants
- Water Treatment and Disinfection
- Soil Management and Crop Yield
- Advanced Machining and Optimization Techniques
- Periodontal Regeneration and Treatments
- Legionella and Acanthamoeba research
- Microbial infections and disease research
- Viral Infections and Vectors
- Genetic Mapping and Diversity in Plants and Animals
Fundação Oswaldo Cruz
2014-2024
Université Paris-Sud
2018-2019
Centre National de la Recherche Scientifique
2018-2019
CEA Paris-Saclay
2018-2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2018-2019
Institut de Biologie Intégrative de la Cellule
2019
Université Paris-Saclay
2018-2019
Pontifícia Universidade Católica de Minas Gerais
2019
MRC Laboratory of Molecular Biology
2014-2015
Universidade Federal de Minas Gerais
2015
Abstract Background The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping widely used PCR-based reverse-hybridization blotting technique that assays genetic diversity this and useful both for clinical laboratory, molecular epidemiology, evolutionary population genetics. It easy, robust, cheap, produces highly diverse portable numerical results, as result...
The current study evaluated Mycobacterium tuberculosis isolates from Rio de Janeiro, Brazil, for genomic deletions. One locus in our panel of PCR targets failed to amplify approximately 30% strains. A single novel long sequence polymorphism (>26.3 kb) was characterized and designated RD(Rio). Homologous recombination between two similar protein-coding genes is proposed as the mechanism deleting or modifying 10 genes, including potentially immunogenic PPE proteins. flanking regions RD(Rio)...
The epidemiologically important Mycobacterium tuberculosis Beijing genotype strains, highly endemic in East Asia, have become an emerging infection certain geographic areas, including Russia, because of its increasing prevalence and association with multidrug resistance (MDR). aim was to verify whether MDR strains circulating the regions present some biological particularities that could contribute their success causing disease comparison sporadic from locations low genotype. We evaluated...
Genotyping of Mycobacterium tuberculosis isolates is a powerful tool for epidemiological control (TB) and phylogenetic exploration the pathogen. Standardized PCR-based typing, based on 15 to 24 mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) loci combined with spoligotyping, has been shown have adequate resolution power tracing TB transmission be useful predicting diverse strain lineages in European settings. Its informative value needs tested high...
ABSTRACT Spoligotyping has shown Mycobacterium tuberculosis strains to be composed of different lineages, and some them are not just geographically restricted but also affect specific ethnic populations associated with outbreaks drug resistance. We recently described a particular subtype within the Latin American-Mediterranean (LAM) family, called RD Rio , widespread in Brazil. Moreover, recent data indicate that is present many countries on all continents cavitary disease multidrug...
We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs) typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs Brazil. High-quality genotypes were obtained both 61 that 185 bovine all these identified as M. by conventional identification procedures. On the basis spoligotyping, 53 grouped into nine...
Rio de Janeiro is endemic for tuberculosis (TB) and presents the second largest prevalence of disease in Brazil. Here, we present bacterial population structure 218 isolates Mycobacterium tuberculosis, derived from 186 patients that were diagnosed between January 2008 December 2009. Genotypes generated by means spoligotyping, 24 MIRU-VNTR typing presence fbpC103, RDRio RD174. The results confirmed earlier data predominant genotypes are those Euro American Lineages (99%). However, observed...
Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), pathogen responsible for serious economic impact on livestock sector. In order to obtain data isolated M. strains and assist in control eradication program BTB, a cross sectional descriptive molecular epidemiology study Brazilian Midwest was conducted. Through spoligotyping 24-loci MIRU-VNTR methods, 37 clinical isolates circulating region were analyzed, 10 from state Mato Grosso, 12 Grosso do Sul 15 Goiás. The analysis...
Introdução: O plasma rico em plaquetas (PRP) é uma terapia promissora na medicina regenerativa, obtida a partir do próprio sangue paciente e fatores de crescimento. Sua aplicação direta área afetada, junto com fibrina rica plaqueta (PRF), promove aceleração processo cicatrização regeneração tecidual. Investigado diversas aplicações clínicas, o PRP PRF desperta crescente interesse comunidade médica científica devido ao seu potencial terapêutico, embora ainda existam desafios serem superados....
Quais as condições que permitem o êxito escolar e a ascensão social de um sujeito origem popular enfrenta pobreza, opressão instabilidades do ambiente familiar, esta última em decorrência adoecimento materno na fase inicial seu desenvolvimento? A partir uma perspectiva análise relato, guiada por discussões teóricas Bernard Charlot, quais tratam da teoria construção relação com saber estudantes popular, pela resiliência Boris Cyrulnik tendo como ferramenta narrativa autorreflexiva actancial...
The main cause of pulmonary tuberculosis (TB) is infection with Mycobacterium (MTB). We aimed to evaluate the contribution nontuberculous mycobacteria (NTM) disease in patients from state Rondônia using respiratory samples and epidemiological data TB cases. isolates were identified a combination conventional tests, polymerase chain reaction-based restriction enzyme analysis hsp65 gene sequencing. Among 1,812 cases suspected having TB, 444 yielded bacterial cultures, including 369 positive...
We aimed to characterize the genetic diversity of drug-resistant Mycobacterium tuberculosis (MTb) clinical isolates and investigate molecular epidemiology multidrug-resistant (MDR) from Minas Gerais State, Brazil. One hundred four MTb were assessed by IS6110-RFLP, 24-locus mycobacterial interspersed repetitive units variable-number tandem repeats (MIRU-VNTR), TB-SPRINT (simultaneous spoligotyping rifampicin-isoniazid drug-resistance mutation analysis) 3R-SNP-typing (analysis...
Abstract Background The classical spoligotyping technique, relying on membrane reverse line-blot hybridization of the spacers Mycobacterium tuberculosis CRISPR locus, is used world-wide (598 references in Pubmed April 8th, 2011). However, until now no inter-laboratory quality control study had been undertaken to validate this technique. We analyzed membrane-based by comparing it recently introduced and highly robust microbead-based spoligotyping. Nine hundred twenty-seven isolates were...
Background Multidrug resistance is a critical factor in tuberculosis control. To gain better understanding of multidrug resistant Brazil, retrospective study was performed to compare genotypic diversity and drug associated mutations Mycobacterium isolates from national reference center. Methods Findings Ninety-nine 12 Brazilian states were studied. Drug-resistance patterns determined the rpoB katG genes screened for mutations. Genotypic investigated by IS6110-RFLP Luminex 47 spoligotyping....
The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region Difference Rio (RDRio) was reported initially Brazil characterized by genomic deletion about 26.3 kb. This lineage seems to show specific adaptation Euro-Latin population. In context, we sought evaluate LAM presence RDRio samples from three countries including...
We analysed 16 variable number tandem repeats (VNTR) and three single-nucleotide polymorphisms (SNP) in Mycobacterium leprae present on 115 Ziehl-Neelsen (Z-N)-stained slides 51 skin biopsy samples derived from leprosy patients Ceará (n = 23), Pernambuco 41), Rio de Janeiro 22) Rondônia (RO) 78). All biopsies yielded SNP-based genotypes, while 48 of the (94.1%) complete VNTR genotypes. evaluated two procedures for extracting M. DNA Z-N-stained slides: first including Chelex other combining...
O objetivo do presente trabalho foi utilizar métodos bacteriológicos e moleculares para a identificação Mycobacteriumbovis em lesões observadas carcaças de bovinos durante inspeção postmortem rotina matadouros-frigoríficos com serviço oficial. Foi acompanhado o abate 825.394 bovinos, sadios ao exame ante mortem pelo oficial dez estado da Bahia. Carcaça 180 apresentaram sugestivas tuberculose por outras linfadenites. No isolamento bacteriano, 25 amostras crescimento disgônico colônias...
Drug-resistant tuberculosis (TB) is a growing global threat. Approximately 450,000 people developed multidrug-resistant TB worldwide in 2012 and an estimated 170,000 died from the disease. This paper describes sociodemographic, clinical-epidemiological bacteriological aspects of correlates these features with distribution anti-TB drug resistance. Mycobacterium (MT) cultures susceptibility testing were performed according to BACTEC MGIT 960 method. The results demonstrated that MT strains...