A5102754889- Immune Response and Inflammation
- Sepsis Diagnosis and Treatment
- Respiratory Support and Mechanisms
- NF-κB Signaling Pathways
- Thermal Regulation in Medicine
- Inflammatory mediators and NSAID effects
- S100 Proteins and Annexins
- Advanced Glycation End Products research
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammasome and immune disorders
- Cell death mechanisms and regulation
- Enhanced Recovery After Surgery
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Traditional Chinese Medicine Analysis
- interferon and immune responses
- Extracellular vesicles in disease
- Advanced Nanomaterials in Catalysis
- Wound Healing and Treatments
- Asthma and respiratory diseases
- Nosocomial Infections in ICU
- Neonatal Respiratory Health Research
Wenzhou City People's Hospital
2024
Jingdong (China)
2024
Chinese PLA General Hospital
2011-2020
First Affiliated Hospital of Chinese PLA General Hospital
2001-2019
National Natural Science Foundation of China
2001
Abstract Introduction To investigate the significance of changes in regulatory T cells (Tregs) activity and its relationship with sepsis, as well outcome patients major burns. Methods The periphery blood samples 106 were collected on post-burn days 1, 3, 7, 14, 21. Tregs isolated their phenotypes (cytotoxic T-lymphocyte-associated antigen 4 forkhead/winged helix transcription factor p3) analyzed by flow cytometry, contents cytokines (interleukin-10 transforming growth factor-β1) released...
Astragalus polysaccharides (APS) isolated from one of the Chinese herbs, mongholicus, are known to have a variety immunomodulatory activities. However, it is not yet clear whether APS can exert an effect on immune functions regulatory T cells (Tregs). This study was carried out investigate function peripheral blood Tregs in postburn sepsis.BALB/C mice were randomly divided into six groups as follows: sham burn group, control (burn without infection animals) plus P. aeruginosa with (50 mg/kg)...
Dendritic cell (DC) can be stimulated by both exogenous pathogen-associated molecular patterns (PAMPs) such as lipopolysaccharide (LPS) and endogenous damage-associated (DAMPs) high mobility group box-1 protein (HMGB1). MicroRNAs (miRNAs) act post-transcriptional fine tuners of mRNA. Studies have focused mostly on the potential role miRNAs in DCs maturation triggered PAMPs, especially LPS, however, little is known about regulatory mechanism underlying effects DC mediated DAMPs, including...
With both in vivo and vitro experiments, the present study was conducted to investigate effect of regulatory T cell (Treg) on promoting T-lymphocyte apoptosis its mechanism through transforming growth factor-beta (TGF-β1) signaling mice. A murine model polymicrobial sepsis reproduced by cecal ligation puncture (CLP); PC61 anti-TGF-β antibodies were used decrease counts CD4+CD25+ Tregs inhibit TGF-β activity, respectively. Splenic CD4+CD25− cells isolated. Phenotypes, including cytotoxic...
Tolerogenic dendritic cells (DCs) are associated with poor prognosis of sepsis. Matrix metalloproteinases (MMPs) have been shown to immunomodulatory effects. However, whether MMPs involved in the functional reprogramming DCs is unknown. The study aims investigate role sepsis-induced tolerance and potential mechanisms. A murine model late sepsis was induced by cecal ligation puncture (CLP). expression levels members MMP family were detected tolerogenic using microarray assessment. roles...
Regulatory T cells (Tregs) appear to be involved in sepsis-induced immune dysfunction; neuropilin-1 (Nrp-1) was identified as a surface marker for <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi mathvariant="normal">C</mml:mi><mml:mi mathvariant="normal">D</mml:mi><mml:msup><mml:mrow><mml:mn mathvariant="normal">4</mml:mn></mml:mrow><mml:mrow><mml:mo>+</mml:mo></mml:mrow></mml:msup><mml:mi...
The present study was conducted to determine the relationship between levels of neopterin and endotoxin in circulation, whether level related development severe sepsis after extensive burns. This prospective included 35 patients with burn size greater than 30% (30–98%), 22 healthy volunteers who served as a comparison group. Neopterin increased most on day 3 post‐burn, but they were not significantly correlated extent surface ( P > 0.05). A high serum found n = 15), marked elevation...
The primary mechanisms of sepsis induced cellular immunosuppression involve immune dysfunction T lymphocytes and negative immunoregulation regulatory cells (Tregs). It has been found that tuftsin is an modulating peptide derived from IgG in spleen. T-peptide one analogs. Herein, we examined the effect on cell-mediated immunity presence lipopolysaccharide (LPS) survival rate septic mice. regulated proliferative ability CD4(+)CD25(-) dual responses. Meanwhile, 10 100 μg/ml T-peptides were able...
We hypothesized that lipopolysaccharide-binding protein (LBP) and lipopolysaccharide receptor CD14 would present a pair of key molecules in pathophysiologic alterations induced by low concentrations endotoxin after trauma. The aim this study was to investigate the relationship between translocation tissue LBP/CD14 messenger ribonucleic acid (mRNA) expression burn injury, define potential role mediating inflammatory mediator induction, as well pathogenesis organ damage.Wistar rats were...
The current experiments were performed to determine the effects of a subtherapeutic dose polymyxin B sulfate on gut origin endotoxemia/bacterial translocation, and tumor necrosis factor (TNF) interleukin-1 (IL-1) release following hemorrhagic shock (30 mm Hg, 90 min) in rats. results showed that significant portal systemic endotoxemia took place control group (portal, 0.269 0.845 endotoxin units (EU)/mL; systemic, 0.164 0.655 EU/mL), but not treatment (except 0.5 hour blood: 0.207 +/- 0.094...
To investigate the potential mechanisms underlying in vivo effect of recombinant bactericidal/permeability-increasing protein (rBPI21) on endogenous bacteria or endotoxin translocation and lipopolysaccharide-binding protein/CD14 expression secondary to thermal injury.Prospective, randomized, controlled animal study.College hospital research laboratory.Thirty-six male Wistar rats weighing 250-300 g.The were anesthetized, a 35% total body surface area full-thickness burn was created. Animals...
BACKGROUND Cellular immunity plays a crucial role in sepsis, and lymphocyte apoptosis is key factor immune homeostasis. Tumor necrosis factor-alpha (TNF-alpha)-induced protein 8-like 2 (TIPE2) suggested to play critical maintaining This study investigated the of TIPE2 CD4⁺ T based on mouse model thermal injury. MATERIAL AND METHODS BALB/c male mice were randomized into 6 groups: sham, burn, burn with siTIPE2, siTIPE2 control, TIPE2, control groups. Splenic lymphocytes collected by use...
The purpose of this study was to explore the causative effects high-velocity bullet injury (5.56 mm, 930 m/sec) combined with hemorrhagic shock on gut-derived endotoxemia and multiple system organ dysfunction or failure. concentration endotoxin in both portal systemic blood markedly increased after gunshot wounds together (p < 0.05-0.01). portal/systemic lipopolysaccharide ratio persistently decreased wound (CW) group at 24 72 hours injury, it much lower CW than that single (SW) group. Also,...
To develop and validate a predictive model of 28-day mortality in sepsis based on lactate dehydrogenase-to-albumin ratio (LAR). Sepsis patients diagnosed the department intensive care medicine First Affiliated Hospital Soochow University from August 1, 2017 to September 2022 were retrospective selected. Clinical data, laboratory indicators, disease severity scores [acute physiology chronic health evaluation II (APACHE II), sequential organ failure assessment (SOFA)] collected. Patients...
The status of cellular immunity has been shown to be associated with the occurrence and development sepsis. Accumulating evidence demonstrated that tumor necrosis factor-α-induced protein 8 like-2 (TIPE2) plays an important role in maintaining homeostasis immune function. present study, use a controlled vivo approach, effect TIPE2 on cell-mediated CD4 + T lymphocytes thermal injury murine model. One hundred twenty-eight male mice were randomly allocated into four groups, which sham burn...
Society of Critical Care Medicine; 28th Educational and Scientific Symposium; San Francisco, California, USA; January 23-27, 1999: Poster Presentations: Hall
Objective To observe the effect of regulatory dendritic cells (DCregs) on burn injury induced proinflammatory cytokine production and mortality rate after a single intraperitoneal injection CD11clowCD45RBhigh DCs to injured mice. Methods DCregs were isolated purified from spleen 100 normal BALB/c mice procure by MiniMACS. Mice subjected 15% total body surface area (TBSA) back. Twenty used, splenic (1×105/ml, 5×105/ml, 10×105/ml) given them investigate protective against lethality at postburn...