A5006499801- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Intraocular Surgery and Lenses
- Immune cells in cancer
- Allergic Rhinitis and Sensitization
- CAR-T cell therapy research
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Supramolecular Chemistry and Complexes
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Phagocytosis and Immune Regulation
- Supramolecular Self-Assembly in Materials
- Asthma and respiratory diseases
- Glaucoma and retinal disorders
- interferon and immune responses
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Corneal Surgery and Treatments
Central South University
2017-2025
Shenzhen Center for Disease Control and Prevention
2023-2025
Xiangya Hospital Central South University
2017-2025
XinHua Hospital
2015-2023
Southeast University
2018-2023
Soochow University
2022-2023
South China University of Technology
2022-2023
Nanjing Medical University
2010-2023
Shanghai Jiao Tong University
2015-2023
First Affiliated Hospital of Soochow University
2023
<h3>Background</h3> Tumor-associated macrophages (TAMs) facilitate tumor progression via establishment of an immunosuppressive microenvironment (TME). However, it is poorly understood how cells could functionally modulate TAMs. Our previous work indicated that cell-released autophagosomes (TRAPs), a type LC3-II<sup>+</sup> double-membrane extracellular vesicles (EVs) was sufficient to suppress anti-tumor immune responses by inducing IL-10-producing B and suppressive neutrophils. Here, we...
Abstract The immunologic effects of chemotherapy-induced tumor cell death are not completely understood. Accumulating evidence suggests that phagocytic clearance apoptotic cells, also known as efferocytosis, is an immunologically silent process, thus maintaining immunosuppressive microenvironment (TME). Here we report that, in the breast microenvironment, thymosin α-1 (Tα-1) significantly reverses M2 polarization IL10-producing tumor-associated macrophages (TAM) during efferocytosis induced...
Minimal residual leukemia cells (MRLs) are difficult to eradicate through traditional treatment and therefore remain be a major threat the long-term survival of patients. Tumor-derived exosomes (TEXs), which carry tumor associated antigens (TAA), may potential cell-free vaccine for specific eradication MRLs. However, TEXs intended less immunogenic due exosomal TGF-β1. To further optimize efficacy TEX-based vaccines, we investigated whether from TGF-β1 silenced (LEXTGF-β1si) had an increased...
<h3>Background</h3> CD4<sup>+</sup> T cells are critical effectors of anti-tumor immunity, but how tumor influence cell effector function is not fully understood. Tumor cell-released autophagosomes (TRAPs) being recognized as modulators host immunity during progression. Here, we explored the mechanistic aspects TRAPs in modulation microenvironment. <h3>Methods</h3> isolated from lines and pleural effusions or ascites cancer patients were incubated with to examine mechanism differentiation...
Mass vaccination represents a highly effective strategy for accelerating disease control while simultaneously reducing incidence and mortality rates. By developing comprehensive plans standards mass vaccination, it is feasible to optimize resource allocation swiftly enhance coverage, thereby preventing, controlling, or interrupting outbreaks epidemics of specific infectious diseases. To standardize the process establish population immunity barrier in an orderly, efficient, safe manner, panel...
Background Copy number variations (CNVs) represent an important type of genetic variation that deeply impact phenotypic polymorphisms and human diseases. The advent high-throughput sequencing technologies provides opportunity to revolutionize the discovery CNVs explore their relationship with However, most existing methods depend on depth show instability low sequence coverage. In this study, using coverage whole-genome (LCS) we have developed effective population-scale CNV calling (PSCC)...
Abstract Background : Tumor cell-derived exosomes (TEXs) have been widely used to induce antitumor immune responses in animal models and clinical trials. Similarly, leukemia (LEXs) can antileukemia models. However, the immunity induced by LEXs is less effective, which may be due an inadequate costimulatory capacity. Methods In this study, we transduced L1210 cells with a lentiviral vector encoding two B7 molecules (CD80, CD86) obtained that highly expressed CD80 CD86. The response derived...
Most breast cancer-related deaths are caused by metastasis in vital organs including the lungs. Development of supportive metastatic microenvironments, referred to as premetastatic niches (PMNs), certain distant before arrival cells, is critical metastasis. However, mechanisms PMN formation not fully clear. Here, we demonstrated that chemoattractant C-C motif chemokine ligand 2 (CCL2) could be stimulated heat shock protein 60 (HSP60) on surface murine 4 T1 cancer cell-released LC3+...
Abstract Purpose To construct and validate a combined nomogram model based on magnetic resonance imaging (MRI) radiomics Albumin-Bilirubin (ALBI) score to predict therapeutic response in unresectable hepatocellular carcinoma (HCC) patients treated with hepatic arterial infusion chemotherapy (HAIC). Methods The retrospective study was conducted 112 HCC who underwent pretherapeutic MRI examinations. Patients were randomly divided into training ( n = 79) validation cohorts 33). A total of 396...
Cucurbit[7]uril-based supramolecular polymer chemistry, which closely integrates host–guest recognition with multifunctional polymeric structures, is a promising toolbox for living cell engineering.
Acetylation of extracellular proteins has been observed in many independent studies where particular attention given to the dynamic change microenvironmental protein post-translational modifications. While can be acetylated within cells prior their micro-environmental distribution, deacetylation a tumor microenvironment remains elusive. Here it is described that multiple acetyl-vWA domain-carrying including integrin β3 (ITGB3) and collagen 6A (COL6A) are deacetylated by Sirtuin family member...
Employing living cells as carriers to transport transition metal-based catalysts for target-specific bio-orthogonal catalysis represents a cutting-edge approach in advancing precision biomedical applications. One of the initial hurdles this endeavor involves effectively attaching carrier while preserving cells' innate ability interact with biological systems and maintaining unaltered catalytic activity. In study, we have developed an innovative layer-by-layer method that leverages...
Aging increases susceptibility to infection, in part because thymic involution culminates reduced naïve T-lymphocyte output. Thymic epithelial cells (TECs) are critical ensure normal maturation of thymocytes and production peripheral T cells. The forkhead-class transcription factor, encoded by FoxN1, regulates development, differentiation, function TECs, both the prenatal postnatal thymus. We recently showed that expression keratin 14 (K14)-expressing is essential for maintenance medullary...
Owing to the outbreak of novel coronavirus (SARS-CoV-2) worldwide at end 2019, development a SARS-CoV-2 vaccine became an urgent need. In this study, we developed type 9 adeno-associated virus vectored candidate expressing dimeric receptor binding domain (RBD) spike protein (S protein) and evaluated its immunogenicity in murine model. The candidate, named AAV9-RBD virus, was constructed by inserting signal peptide N-terminus two copies RBD, spaced linker, into genome virus. vitro assays...
Abstract Tumor cell-released LC3 + extracellular vesicles (LC3 EVs) participate in immunosuppression during autophagy and contribute to the occurrence development of breast cancer. In view strong association between EVs cancer, developing an effective strategy for quantitative detection levels with high sensitivity identify as new biomarkers accurate diagnosis cancer is crucial, but yet not been reported. Herein, ultrasensitive electrochemical immunosensor presented determination using a...
A simple and effective strategy was developed to enrich ubiquitinated proteins (UPs) from cancer cell lysate using the α-Al2O3 nanoparticles covalently linked with ubiquitin binding protein (Vx3) (denoted as α-Al2O3–Vx3) via a chemical linker. The functionalized α-Al2O3–Vx3 showed long-term stability high efficiency for enrichment of UPs lysates. Flow cytometry analysis results indicated dendritic cells (DCs) could more effectively phagocytize α-Al2O3–Vx3-UPs than physical mixture Vx3-UPs...