A5054197976- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Endometrial and Cervical Cancer Treatments
- Cancer Cells and Metastasis
- FOXO transcription factor regulation
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Cancer Mechanisms and Therapy
- Barrier Structure and Function Studies
- Cervical Cancer and HPV Research
- Estrogen and related hormone effects
- Endometriosis Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Salivary Gland Tumors Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Galectins and Cancer Biology
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2011-2024
University of Brescia
2015-2024
Institute of Molecular Medicine
2011-2019
Mario Negri Institute for Pharmacological Research
2013
University of Milano-Bicocca
2013
Yale University
2011
Brescia University
2009
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2009
New York University
2009
Columbia University Irving Medical Center
2009
Uterine serous carcinoma (USC) is a biologically aggressive subtype of endometrial cancer. We analyzed the mutational landscape USC by whole-exome sequencing 57 cancers, most which were matched to normal DNA from same patients. The distribution number protein-altering somatic mutations revealed that 52 tumors had fewer than 100 (median 36), whereas 5 more 3,000 mutations. in these latter showed hallmarks defects mismatch repair. Among remainder, we found significantly increased burden...
Significance Some cancers, termed carcinosarcomas (CSs), have mixed cell types, with either epithelial or mesenchymal features. Sequencing the genomes of uterine and ovarian CSs demonstrated that these different types derive from a common precursor has many mutations typical cancers. In addition, we find tumors significant burden point amplification histone genes, suggesting potential role in sarcomatous transformation. Consistent this finding, expression specific gene carcinoma cells...
Abstract With the goal of identifying genes with a differential pattern expression between ovarian serous papillary carcinomas (OSPCs) and normal (NOVA) epithelium using this knowledge for development novel diagnostic therapeutic markers cancer, we used oligonucleotide microarrays probe sets complementary to 12,533 analyze gene profiles 10 primary OSPC cell lines, 2 established lines (UCI‐101, UCI‐107) 5 NOVA epithelial cultures. Unsupervised analysis data identified 129 170 that exhibited...
The soy isoflavone genistein targets adipose tissue and elicits physiological effects that may vary based on dietary intake. We hypothesized the of are dose gender dependent. Four-week-old C57BL/6 male female mice received daily oral doses (50–200,000 μg/kg·d) or 17β-estradiol (E2) (5 for 15 d a diet containing 800 ppm genistein. Genistein increased epididymal renal fat pad adipocyte size at up to 50,000 μg/kg·d in males but not females. alteration adipocity correlated with changes...
To date, no good marker for screening or disease monitoring of endometrial cancer (EC) is available. The aims this study were to investigate HE4 gene, protein expression and serum (sHE4) levels in a panel ECs normal endometria (NEs) correlate sHE4 with patient clinicopathological characteristics prognosis.Using quantitative real-time PCR we tested 46 20 NEs gene expression. Protein was analysed by immunohistochemistry on tissue microarrays 153 33 NEs. Pre-operative samples from 138 EC 76 NE...
Abstract Background: The aim of this work was to analyze the diagnostic and prognostic value serum human epididymis protein 4 (HE4) Risk for Ovarian Malignancy Algorithm (ROMA) in epithelial ovarian cancer (EOC). Methods: Preoperative samples 419 women (140 healthy controls, 131 benign cysts, 34 endometriosis, 114 EOC) were tested CA125 HE4 using fully automated methods (Abbott ARCHITECT) validated cutoff values. Results: For discrimination masses from EOC, premenopausal women, sensitivity...
Uterine leiomyosarcomas (uLMS) are aggressive tumors arising from the smooth muscle layer of uterus. We analyzed 83 uLMS sample genetics, including 56 Yale and 27 The Cancer Genome Atlas (TCGA). Among them, a total 55 samples two patient-derived xenografts (PDXs) TCGA have whole-exome sequencing (WES) data; 10 RNA-sequencing (RNA-Seq) 11 whole-genome (WGS) data. found recurrent somatic mutations in TP53, MED12, PTEN genes. Top mutated genes included ATRX, PTEN, MEN1 Somatic copy number...
The purpose of this study was to assess the diagnostic and prognostic impact preoperative serum determination human epididymis protein 4 (sHE4), investigate its potential correlation with clinicopathological features survival endpoints in endometrial cancer patients.Preoperative samples from 193 patients 125 women normal endometrium were measured for sHE4 CA125 (sCA125) concentrations by quantitative chemiluminescent microparticle immunoassays on automated Architect instrument.sHE4...
Significance Ovarian cancer has the propensity for early dissemination of microscopic metastases, making detection challenging. Development treatments patients with advanced/recurrent chemotherapy-resistant disease remains an unmet need. We sequenced primary, synchronous bilateral ovarian (SBOC), metastatic, and recurrent tumors. found primary metastatic tumors to be remarkably similar SBOC clonally related in all cases, suggesting represent intrinsic feature cancer. c-MYC PIK3CA...
Purpose: Stage I epithelial ovarian cancer (EOC) represents about 10% of all EOCs and is characterized by good prognosis with fewer than 20% patients relapsing. As it occurs less frequently advanced-stage EOC, its molecular features have not been thoroughly investigated. We demonstrated that in stage EOC miR-200c-3p can predict patients' outcome. In the present study, we analyzed expression long non-coding RNAs (lncRNA) to enable potential definition a transcriptional signature prognostic...
The prognosis of advanced/recurrent cervical cancer patients remains poor. We analyzed 54 fresh-frozen and 15 primary cell lines, along with matched-normal DNA, by whole-exome sequencing (WES), most which harboring Human-Papillomavirus-type-16/18. found recurrent somatic missense mutations in 22 genes (including PIK3CA, ERBB2, GNAS) a widespread APOBEC cytidine deaminase mutagenesis pattern (TCW motif) both adenocarcinoma (ACC) squamous carcinomas (SCCs). Somatic copy number variants (CNVs)...
Survival rates of oral squamous cell carcinoma (OSCC) remained substantially unchanged over the last decades; thus, additional prognostic tools are strongly needed.Salivary miRNAs have emerged as excellent non-invasive cancer biomarker candidates, but their association with OSCC prognosis has not been investigated yet.In this study, we analyzed global salivary miRNA expression in patients and healthy controls, aim to define its diagnostic potential.Methods: Saliva was collected from newly...
Ovarian carcinomas (OCs) are poorly immunogenic and immune checkpoint inhibitors (ICIs) have offered a modest benefit. In this study, high CD3+ T-cells CD163+ tumor-associated macrophages (TAMs) densities identify subgroup of infiltrated high-grade serous (HGSCs) with better outcomes superior response to platinum-based therapies. On the contrary, in most clear cell (CCCs) showing poor prognosis refractory platinum, TAM density is associated low T frequency. Immune HGSC characterized by...
Epithelial ovarian cancer (EOC) is one of the most lethal gynecologic diseases, with survival rate virtually unchanged for past 30 years. EOC comprises different histotypes molecular and clinical heterogeneity, but up till now present gold standard platinum-based treatment has been conducted without any patient stratification. The aim study to generate microRNA (miRNA) profiles characteristic each stage I histotype, identify subtype-specific biomarkers improve our understanding underlying...
Epithelial ovarian cancer (EOC) is a spectrum of different diseases, which makes their treatment challenge. Forkhead box M1 (FOXM1) an oncogene aberrantly expressed in many solid cancers including serous EOC, but its role non-serous EOCs remains undefined. We examined FOXM1 expression and correlation to prognosis across the three major EOC subtypes, tumorigenesis chemo-resistance vitro.Gene signatures were generated by microarray for 14 clear-cell 26 endometrioid EOCs, 15 normal endometrium...
Endometrial cancer is the most common gynecologic malignancy in developed countries. Trop-2 a glycoprotein involved cellular signal transduction and differentially overexpressed relative to normal tissue variety of human adenocarcinomas, including endometrioid endometrial carcinomas (EEC). overexpression has been proposed as marker for biologically aggressive tumor phenotypes.Trop-2 protein expression was quantified using microarrays consisting formalin-fixed paraffin-embedded specimens from...