A5101762323- Sperm and Testicular Function
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Protease and Inhibitor Mechanisms
- Urologic and reproductive health conditions
- Renal and related cancers
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- Testicular diseases and treatments
- Calpain Protease Function and Regulation
- Bladder and Urothelial Cancer Treatments
- Reproductive Health and Technologies
- Ovarian function and disorders
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Signaling Pathways in Disease
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Parathyroid Disorders and Treatments
- Urological Disorders and Treatments
- Medical Imaging and Pathology Studies
- Seed Germination and Physiology
- Hormonal and reproductive studies
Jiangsu Province Hospital
2010-2024
Nanjing Medical University
2010-2024
Takenaka (Japan)
2017
University of North Carolina at Chapel Hill
2005-2007
Human sperm cryopreservation in assisted reproductive technology is the only proven method that enables infertile men to father their own children. However, freezing and thawing reduces spermatozoon motility, viability, fertilizing ability. An association between dysfunctional spermatozoa due cryoinjury protein changes has not been established. We investigated through proteomic analysis differential characteristics freeze-thawed fresh samples obtained from nine normozoospermic donors....
Abstract Chronic allograft dysfunction (CAD) induced by kidney interstitial fibrosis is the main cause of failure in transplantation. Endothelial‐to‐mesenchymal transition (EndMT) may play an important role fibrosis. We, therefore, undertook this study to characterize functions and potential mechanism EndMT transplant Proteins mRNAs associated with were examined human umbilical vein endothelial cells (HUVECs) treated transforming growth factor‐beta1 (TGF‐β1) at different doses or intervals...
The purpose of this study was to describe the first metabolic profile human sperm cells through application an untargeted platform based on gas chromatography-mass spectrometry (GC-MS). Sperm cell samples from patients diagnosed with idiopathic asthenozoospermia (n = 30) and healthy subjects were analyzed using a nontargeted metabolomics method GC-MS spectroscopy. mass spectrometric data collected multivariate univariate analyses identify metabolites related asthenozoospermia. By metabolomic...
Eppin (SPINLW1; GeneID, 57119) is a single-copy gene encoding cysteine-rich protein found only in the testis and epididymis, which contains both Kunitz-type WAP-type four disulfide core protease inhibitor consensus sequences. This study demonstrates that, seminal plasma on human spermatozoa following ejaculation, bound to semenogelin I (Sg). Six different experimental approaches: 1) immunoprecipitation from with anti-Eppin, 2) colocalization semen spermatozoa, 3) incubation of recombinant...
The aberrant expression of microRNAs (miRs) has been identified to serve a crucial role in tumor progression. present study aimed evaluate the miR-126 human prostate cancer (PCa). Firstly, tissues and cell lines was analyzed. A luciferase reporter assay rescue were performed, which ADAM metalloproteinase domain 9 (ADAM9) as target gene miR-126. Subsequently, Kaplan-Meier log-rank analyses used investigate association between ADAM9 PCa prognosis. results revealed that significantly...
Antisense long noncoding RNAs (lncRNAs) are reported to play a regulating role in carcinogenesis of various human malignancies. However, the function lncRNAs and their underlying mechanism renal cell carcinoma (RCC) is still unknown. The aims this study investigate expression lncRNA BX357664 RCC explore its lines. As result, was downregulated according previous microarray analysis qualitative real-time polymerase chain reaction. After upregulation BX357664, reduced migration, invasion,...
Deficiencies in tricarboxylic acid (TCA) cycle enzymes have been shown to cause a wide spectrum of human diseases, including malignancies and neurological cardiac diseases. In mammalian spermatozoa mitochondria, the TCA is known be crucial metabolic pathway that contributes produce ATP. There little about role mechanism mitochondrial aconitase (ACO2), which an important regulatory enzyme cycle, asthenozoospermia. current study, immunofluorescence staining localized ACO2 sperm mid-piece. By...
Abstract This study aimed to explore the association between methylation status of VDAC2 gene promoter region and idiopathic asthenospermia (IAS). Twenty-five IAS patients 27 fertile normozoospermia (NZ) were involved. GC-2spd cells treated with different concentrations 5-aza-2′-deoxycytidine (5-Aza-CdR) for 24 h 48 h. qRT-PCR was conducted reveal whether or not expression regulated by methylated modification. A dual-luciferase activity detection used verify in cells. Bisulphite genomic...
The calpain 2 (CAPN2) is upregulated in various malignant carcinomas. Previous studies have reported that CAPN2 functioned as an oncogenic factor human cancers. However, its clinical role and potential effects on cell metastasis proliferation renal carcinoma (RCC) remain unknown. In this study, we evaluated the mRNA protein levels of RCC specimens, matched normal lines using quantitative Real-time PCR (RT-PCR) western blot. Immunohistochemistry 74 tissues a tissue microarrays (TMAs) kidney...
The mRNA expression of CAPN2 was upregulated in CRPC cells (DU145 and PC3) than that non-CRPC cells. Silencing could inhibit DU145 PC3 proliferation by cell cycle arrest at G1 phase. Knockdown CPAN2 level suppressed the migration invasion capacity reducing matrix metalloproteinase-2 (MMP-2) MMP-9 activation, as well repressing phosphorylation protein AKT mTOR. In addition, we found elevated Pca tissues normal control tissues. Therefore, showed important roles development progression cells,...
// Jundong Zhu 1, * , Chao Liang Yibo Hua Chenkui Miao 1 Jianzhong Zhang Aiming Xu Kai Zhao Shouyong Liu Ye Tian Huiyu Dong Pu Li Shifeng Su Qin and Zengjun Wang Department of Urology, The First Affiliated Hospital Nanjing Medical University, Nanjing, China These authors contributed equally to this work Correspondence to: Wang, email: zengjunwang@njmu.edu.cn Qin, 13776678978@163.com Keywords: CD82/KAI1, tetraspanin, migration/invasion, TGF-β1/Smad signaling, renal cell carcinoma Received:...
Differences in intravaginal ejaculation latency reflect normal biological variation, but the causes are poorly understood. Here, we investigated whether variation an experimental rat model is related to altered sympathetic nervous system (SNS) activity and expression of N-methyl-D-aspartic acid (NMDA) receptors paraventricular nucleus hypothalamus (PVN). Male rats were classified as "sluggish," "normal," "rapid" ejaculators on basis frequency during copulatory behavioral testing. The lumbar...
Semen cryopreservation is widely used in assisted reproductive technologies, but it reduces sperm quality dramatically. The aim of this study was to develop a model using basal semen predict the outcome postthaw parameters and improve efficiency human bank. Basal 180 samples were evaluated first stage, multiple logistic regression analysis involving backward elimination selection procedure applied select independent predictors. After comprehensive all results, we developed new assess...
Background The assessment of left ventricular (LV) remodeling and its association with mineral bone disorder (MBD) in kidney transplant recipients (KTRs) have not been systematically studied. We aimed to evaluate LV changes one year after transplantation (KT) identify their influencing factors.
Interdigitating dendritic cell sarcoma (IDCS) and retroperitoneal leiomyosarcoma are rare tumors. The optimal diagnosis, treatment prognosis remain unknown. current case report presents a 46-year-old male who exhibited with left renal mass combined periprostatic mass. patient underwent surgery twice, respectively for the resection of two masses. postoperative pathological examination confirmed diagnosis IDCS presenting in kidney pelvis. To best our knowledge, it is first appearance same patient.
Recently, the functional polymorphisms in Cyclin D1 (CCND1) have been shown potential influence to risk of renal cell cancer (RCC). Therefore, present study was performed investigate whether these could susceptibility RCC. Four potentially CCND1 (rs1944129, rs7177, rs9344 and rs678653) were genotyped this hospital-based case-control study, comprising 1,488 RCC patients 1,677 cancer-free controls a Chinese population by TaqMan assay. The logistic regression used assess associations between We...