A5107998643- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Commutative Algebra and Its Applications
- Vibrio bacteria research studies
- Algebraic structures and combinatorial models
- Algebraic Geometry and Number Theory
- Animal Virus Infections Studies
- Helicobacter pylori-related gastroenterology studies
- Clostridium difficile and Clostridium perfringens research
- Nosocomial Infections in ICU
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- Antibiotics Pharmacokinetics and Efficacy
- Polynomial and algebraic computation
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Helminth infection and control
- Essential Oils and Antimicrobial Activity
- Phytochemistry and Bioactive Compounds
- Parasite Biology and Host Interactions
- Pneumonia and Respiratory Infections
- Virology and Viral Diseases
- Wildlife Ecology and Conservation
National Hospital of Pediatrics
2023
Hospital for Tropical Diseases
2011-2023
Oxford University Clinical Research Unit
2012-2023
Hue Central Hospital
2019
Meiji University
2013-2015
Dong Thap Hospital
2015
Thai Nguyen University
2013
Thai Nguyen University Of Education
2013
University of Oxford
2010-2013
University of California, San Francisco
2012
Available incidence data for invasive salmonella disease in sub-Saharan Africa are scarce. Standardised, multicountry required to better understand the nature and burden of Africa. We aimed measure adjusted estimates typhoid fever non-typhoidal (iNTS) Africa, antimicrobial susceptibility profiles causative agents.We established a systematic, standardised surveillance blood culture-based febrile illness 13 African sentinel sites with previous reports fever: Burkina Faso (two sites), Ethiopia,...
This study describes the pattern and extent of drug resistance in 1,774 strains Salmonella enterica serovar Typhi isolated across Asia between 1993 2005 characterizes molecular mechanisms underlying reduced susceptibilities to fluoroquinolones these strains. For 1,393 collected southern Vietnam, proportion multidrug has remained high since (50% 2004) there was a dramatic increase nalidixic acid (4%) (97%). In cross-sectional sample 381 from 8 Asian countries, Bangladesh, China, India,...
Significance Shigella sonnei is a globally emerging agent of bacterial dysentery. Here, we use genomics to examine the microevolution S. in Vietnam. We show that was introduced into Vietnam early 1980s, where it continued evolve, spreading geographically establish localized founder populations. The population Ho Chi Minh City has undergone several clonal replacement events, during which small number microevolutionary changes have risen dominance. These changes, induced by horizontal gene...
Background. Trypanosoma is a genus of unicellular parasitic flagellate protozoa. brucei species and cruzi are the major agents human trypanosomiasis; other can cause disease, but rare. In March 2015, 38-year-old woman presented to healthcare facility in southern Vietnam with fever, headache, arthralgia. Microscopic examination blood revealed infection Trypanosoma.
Abstract Background Shigellosis remains considerable public health problem in some developing countries. The nature of Shigellae suggests that they are highly adaptable when placed under selective pressure a human population. This is demonstrated by variation and fluctuations serotypes antimicrobial resistance profile organisms circulating differing setting endemic locations. Antimicrobial the genus Shigella constant threat, with reports Asia being resistant to multiple antimicrobials new...
BackgroundDrug resistant typhoid fever is a major clinical problem globally. Many of the first line antibiotics, including older generation fluoroquinolones, ciprofloxacin and ofloxacin, are failing.ObjectivesWe performed randomised controlled trial to compare efficacy safety gatifloxacin (10 mg/kg/day) versus azithromycin (20 as once daily oral dose for 7 days treatment uncomplicated in children adults Vietnam.MethodsAn open-label multi-centre with pre-specified per protocol analysis...
We performed a prospective multicenter study to address the lack of data on etiology, clinical and demographic features hospitalized pediatric diarrhea in Ho Chi Minh City (HCMC), Vietnam. Over 2,000 (1,419 symptomatic 609 non-diarrheal control) children were enrolled three hospitals over 1-year period 2009–2010. Aiming detect panel pathogens, we identified known diarrheal pathogen stool samples from 1,067/1,419 (75.2%) with 81/609 (13.3%) without diarrhea. Rotavirus predominated (664/1,419;...
Antimicrobial-resistant pathogenic members of the Enterobacteriaceae are a well-defined global problem. We hypothesized that one main reservoirs dissemination antimicrobial resistance genes in Vietnam is non-pathogenic intestinal flora, and sought to isolate antimicrobial-resistant organisms from hospitalized patients non-hospitalized healthy individuals Ho Chi Minh City. The results identified substantial faecal carriage gentamicin-, ceftazidime- nalidixic acid-resistant both individuals. A...
Journal Article The decline of typhoid and the rise non-typhoid salmonellae fungal infections in a changing HIV landscape: bloodstream infection trends over 15 years southern Vietnam Get access Tran Vu Thieu Nga, Nga aThe Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, 190 Ben Ham Tu, Quan 5, Ho Chi Minh City, Search other works by this author on: Academic PubMed Google Scholar Christopher M. Parry, Parry VietnambAngkor...
Ventilator-associated pneumonia (VAP) is a serious healthcare-associated infection that affects up to 30 % of intubated and mechanically ventilated patients in intensive care units (ICUs) worldwide. The bacterial aetiology corresponding antimicrobial susceptibility VAP highly variable, can differ between countries, national provinces even different wards the same hospital. We aimed understand document changes causative agents their profiles retrospectively over an 11 year period major...
Clostridium tetani is the etiologic agent of muscle-spasming disease tetanus. Despite an effective vaccine, tetanus ongoing problem in some developing countries. Diagnosis by bacterial culture not done because it generally unnecessary and entry route bacteria can be inapparent. We attempted to isolate evaluate C. from wounds 84 patients with effectively isolated 45 patients. All strains tested positive polymerase chain reaction for gene encoding neurotoxin. Antimicrobial susceptibilities...
Background Plasmid mediated antimicrobial resistance in the Enterobacteriaceae is a global problem. The rise of CTX-M class extended spectrum beta lactamases (ESBLs) has been well documented industrialized countries. Vietnam representative typical transitional middle income country where infectious diseases combined with spread drug shifting and bringing new healthcare challenges. Methodology We collected hospital admission data from pediatric population attending for tropical Ho Chi Minh...
Background Typhoid fever remains a public health problem in Vietnam, with significant burden the Mekong River delta region. is caused by bacterial pathogen Salmonella enterica serovar Typhi (S. Typhi), which frequently multidrug resistant reduced susceptibility to fluoroquinolone-based drugs, first choice for treatment of typhoid fever. We used GoldenGate (Illumina) assay type 1,500 single nucleotide polymorphisms (SNPs) and analyse genetic variation S. isolated from 267 patients region...
Abstract A novel protein translocation system, the type-6 secretion system (T6SS), may play a role in virulence of Campylobacter jejuni. We investigated 181 C. jejuni isolates from humans, chickens, and environmental sources Vietnam, Thailand, Pakistan, United Kingdom for T6SS. The marker was most prevalent human chicken Vietnam.
ABSTRACT Infection by Shigella spp. is a common cause of dysentery in Southeast Asia. Antimicrobials are thought to be beneficial for treatment; however, antimicrobial resistance becoming widespread. We aimed assess the frequency and mechanisms associated with decreased susceptibility azithromycin Asian isolates use these data appropriate breakpoints. recovered Vietnam Laos were screened (15 μg) disc diffusion MIC. Phenotypic was confirmed PCR amplification macrolide loci. compared genetic...
Leptospirosis is a zoonosis known to be endemic in the Mekong Delta of Vietnam, even though clinical reports are uncommon. We investigated leptospira infection rats purchased food markets during rainy season (October) (n=150), as well those trapped dry (February–March) (n=125) region using RT-PCR for lipL32 gene, confirmed by 16S rRNA, microscopic agglutination test (MAT). Results were compared with serovar distribution human cases referred from Ho Chi Minh City hospitals (2004–2012) MAT...
Background The emergence of drug resistant typhoid fever is a major public health problem, especially in Asia. An oral single dose vaccine would have advantages. M01ZH09 live candidate containing Salmonella enterica serovar Typhi (Ty2 aroC− ssaV−) ZH9 with two independently attenuating deletions. Studies healthy adults demonstrated immunogenicity and an acceptable safety profile. Objectives We conducted randomised placebo controlled, single-blind trial to evaluate the Vietnamese children...
ABSTRACT Infections with Salmonella enterica serovar Typhi isolates that have reduced susceptibility to ofloxacin (MIC ≥ 0.25 μg/ml) or ciprofloxacin 0.125 been associated a delayed response clinical failure following treatment these antimicrobials. These are not detected as resistant using current disk breakpoints. We examined 816 of S. from seven Asian countries. Screening for nalidixic acid resistance 16 identified an MIC ≥0.25 μg/ml sensitivity 97.3% (253/260) and specificity 99.3%...
Rotavirus (RoV) and Norovirus (NoV) are the main causes of viral gastroenteritis. Currently, there is no validated multiplex real-time PCR that can detect quantify RoV NoV simultaneously. The aim study was to develop, validate, internally control a one-step RT in stool samples. sensitivity assessed by comparing amplification against current gold standard, enzyme immunoassay (EIA), on samples from 94 individuals with diarrhea without diarrhea. detected 10% more positive than EIA stools...
Data concerning intensive care unit (ICU)-acquired bacterial colonization and infections are scarce from low middle-income countries (LMICs). ICU patients in these settings at high risk of becoming colonized infected with antimicrobial-resistant organisms (AROs). We conducted a prospective observational study the Ho Chi Minh City Hospital for Tropical Diseases, Vietnam November 2014 to January 2016 assess ICU-acquired infections, focusing on five major pathogens our setting: Staphylococcus...
Abstract We performed a case–control investigation to identify risk factors for norovirus infections among children in Vietnam. Of samples from 1,419 who had diarrhea and 609 were asymptomatic, 20.6% 2.8%, respectively, positive. Risk included residential crowding symptomatic contacts, indicating person-to-person transmission of norovirus.
A survey of Bartonella spp. from 275 rats purchased in food markets (n=150) and trapped different ecosystems (rice field, forest, animal farms) (n=125) was carried out during October, 2012–March, 2013, the Mekong Delta Vietnam. The overall prevalence detected by culture PCR blood 14.9% (10.7–19.1%), highest corresponding to Rattus tanezumi (49.2%), followed norvegicus (20.7%). Trapped were also investigated for presence type chiggers (larvae trombiculid mites), on chigger pools collected...
Commercial small-scale chicken farms managed as all-in-all-out but operating with low standards of hygiene/biosecurity are increasingly common in Vietnam. These conditions facilitate the transmission gastrointestinal helminths. However, there no published data on helminths these systems. We aimed (1) to determine prevalence/burden commercial flocks Mekong Delta region and (2) investigate association between worm burdens birds' weight disease status. Randomly selected chickens (n = 120) from...
Utilization of plant resources for treatment Helicobacter pylori infections is one the appealing approaches as rapid emergence antibiotic-resistant strains occurring throughout world. Ethanol extract and its fractions from Hibiscus rosa-sinensis red flower were assessed antibacterial urease inhibitory activities towards forty-three clinical two reference H. pylori. The ethyl acetate fraction exhibited most potent bacteriostatic activity with minimum concentrations (MICs) 0.2-0.25 mg/mL...