A5057703185- Malaria Research and Control
- Mosquito-borne diseases and control
- Plant Pathogens and Fungal Diseases
- Research on Leishmaniasis Studies
- Toxoplasma gondii Research Studies
- Antifungal resistance and susceptibility
- Bacterial Identification and Susceptibility Testing
- Vector-borne infectious diseases
- Insect Resistance and Genetics
- Nail Diseases and Treatments
- Trypanosoma species research and implications
- Digital Imaging for Blood Diseases
- Rabies epidemiology and control
- Viral Infections and Vectors
- Insect and Pesticide Research
- Fungal Infections and Studies
- Lichen and fungal ecology
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Leptospirosis research and findings
- Mycotoxins in Agriculture and Food
- Invertebrate Immune Response Mechanisms
- Bartonella species infections research
- Gut microbiota and health
- Cytomegalovirus and herpesvirus research
Inserm
2014-2024
Sorbonne Université
2019-2024
Institut Pierre Louis d‘Épidémiologie et de Santé Publique
2019-2024
Assistance Publique – Hôpitaux de Paris
2019-2024
Pitié-Salpêtrière Hospital
2019-2024
Sorbonne University Abu Dhabi
2022
Centre Hospitalier Universitaire de Bordeaux
2022
University of French Guiana
2017-2020
Amazon (United States)
2020
London Rebuilding Society
2019
Abstract Malaria infections occurring below the limit of detection standard diagnostics are common in all endemic settings. However, key questions remain surrounding their contribution to sustaining transmission and whether they need be detected targeted achieve malaria elimination. In this study we analyse a range datasets quantify density, detectability, course infection infectiousness subpatent infections. Asymptomatically infected individuals have lower parasite densities on average low...
Invasive pulmonary aspergillosis is a complication in critically ill patients with acute respiratory distress syndrome, especially those severe influenza pneumonia. We report fatal case of invasive an immunocompetent patient France who had coronavirus disease-associated
Mosquito-borne diseases like malaria are rising globally, and improved mosquito vector surveillance is needed. Survival of Anopheles mosquitoes key for epidemiological monitoring transmission evaluation control strategies targeting longevity, as the risk pathogen increases with age. However, available tools to estimate field age often approximate time-consuming. Here, we show a rapid method that combines matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry deep...
ABSTRACT Human leishmaniases are widespread diseases with different clinical forms caused by about 20 species within the Leishmania genus. identification is relevant for therapeutic management and prognosis, especially cutaneous mucocutaneous forms. Several methods available to identify from culture, but they have not been standardized majority of currently described species, exception multilocus enzyme electrophoresis. Moreover, these techniques expensive, time-consuming, in all...
In Mali, Plasmodium falciparum malaria is highly endemic and remains stable despite the implementation of various control measures. Understanding P. population structure variations across country could provide new insights to guide programmes. this study, genetic diversity in regions varying patterns transmission Mali were analysed. A total 648 blood isolates adsorbed onto filter papers during surveillance surveys (December 2012–March 2013, October 2013) four distinct sites screened for...
Abstract Background American cutaneous leishmaniasis (ACL) is endemic in French Guiana. Its epidemiology evolving, notably because of immigration, anthropization natural areas, and new microbiological methods . Our first objective was to update epidemiological data. second look for risk factors ACL. Methods This multicentric study conducted from October 2017 June 2018 Patients with suspicion mucocutaneous were included case positive smear, culture, or PCR‐RFLP on skin biopsy Results One...
Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult species, using the legs or cephalothorax. The protein repertoire from arthropods can vary according compartment, but there no general consensus regarding anatomic part be used. Methods To determine body mosquitoes best suited field specimens, a...
Phlebotomine sand flies are insects that highly relevant in medicine, particularly as the sole proven vectors of leishmaniasis. Accurate identification fly species is an essential prerequisite for eco-epidemiological studies aiming to better understand disease. Traditional morphological painstaking and time-consuming, molecular methods extensive screening remain expensive. Recent have shown matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) a...
Background:Toxoplasma gondii is a parasite of worldwide importance but its burden in indigenous communities remains unclear. In French Guiana, atypical strains T. originating from complex rainforest cycle involving wild felids have been linked to severe infections humans. These cases Amazonian toxoplasmosis are sporadic and outbreaks rarely described. We report on the investigation an outbreak acute remote Amerindian village. discuss causes consequences this emergence. Methods: May 2017,...
Malaria elimination in Southeast Asia remains a challenge, underscoring the importance of accurately identifying malaria mosquitoes to understand transmission dynamics and improve vector control. Traditional methods such as morphological identification require extensive training cannot distinguish between sibling species, while molecular approaches are costly for screening. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged rapid...
Abstract Vector control programmes are a strategic priority in the fight against malaria. However, vector interventions require rigorous monitoring. Entomological tools for characterizing malaria transmission drivers limited and difficult to establish field. To predict Anopheles of transmission, such as mosquito age, blood feeding Plasmodium infection, we evaluated artificial neural networks (ANNs) coupled matrix-assisted laser desorption ionization-time flight (MALDI-TOF) mass spectrometry...
Amazonian toxoplasmosis is a recently described form of Toxoplasma gondii infection, characterized by severe clinical and biological features involvement atypical genetic strains circulating through forest-based cycle. Though mostly reported in French Guiana since 1998, this disease probably under-diagnosed other areas South America. Few data are available on its specific children.We retrospectively included all children seen Cayenne between 2002 2017, diagnosed with acute due to an strain....
Recent studies have highlighted the interest in noninvasive sampling procedures coupled with real-time PCR methods for detection of Leishmania species South America. In French Guiana, method still relied on skin biopsies. Noninvasive protocols should be tested a large annual cohort to improve routine laboratory diagnosis cutaneous leishmaniasis. Therefore, we evaluated performance new and identification protocol involving cotton swabs SYBR green-based Hsp70 gene, Sanger sequencing. Between...
We describe the isolation and characterization of Fusarium volatile from a bronchoalveolar lavage (BAL) sample female patient living in French Guiana with underlying pulmonary infections. Phylogenetic analysis fragments calmodulin (cmdA), translation elongation factor (tef1), RNA polymerase second largest subunit (rpb2), β-tubulin (tub) loci revealed that strain CBS 143874 was closely related to isolate NRRL 25615, known but undescribed phylogenetic species belonging African clade fujikuroi...
Online MALDI-TOF mass spectrometry applications, such as MSI-2, have been shown to help identify dermatophytes, but recurrent errors are still observed between phylogenetically close species. The objective of this study was assess different approaches reduce the occurrence by adding new reference spectra MSI-2 application. Nine libraries were set up, comprising an increasing number obtained from strains that submitted various culture durations on two distinct media: Sabouraud gentamicin...
Identifying fungal clones propagated during outbreaks in hospital settings is a problem that increasingly confronts biologists. Current tools based on DNA sequencing or microsatellite analysis require specific manipulations are difficult to implement the context of routine diagnosis. Using deep learning classify mass spectra obtained identification fungi by MALDI-TOF spectrometry could be interest differentiate isolates belonging epidemic from others. As part management nosocomial outbreak...
We evaluated the use of positive malaria rapid diagnostic tests (mRDTs) to determine genetic diversity Plasmodium falciparum in Mali. Genetic was assessed via multiple loci variable number tandem repeats analysis (MLVA). performed DNA extraction from 104 and 30 negative used mRDTs that had been stored at ambient temperature for up 14 months. Extracted analyzed quantitative polymerase chain reaction (qPCR), MLVA genotyping then on qPCR samples. Eighty-three (83/104, 79.8%) none were confirmed...
Trichosporonosis is a rare invasive infection in humans mainly due to Trichosporon asahii, and especially recovered from patients having haematological malignancy. Since 2012, IGS1 region sequencing used as genotyping method distinguish isolates, with high frequency of one haplotype worldwide geographic specificity for some haplotypes.We compared the whole genome (WGS) study relationship between clinical isolates involved two grouped cases France.IGS1 antifungal susceptibility testing were...
Accurate tools for Toxoplasma gondii detection and quantification can be valuable the early effective management of toxoplasmosis. Droplet digital PCR (ddPCR) is a next-generation end-point technique with high performance. The objective study was to evaluate performance ddPCR absolute T. gondii. From January 2019 October 2020, DNA samples collected at Laboratory Parasitology Mycology Pitié-Salpêtrière Hospital in Paris were retrospectively analyzed by real-time quantitative (qPCR). To detect...
Abstract Malaria control in South-East Asia remains a challenge, underscoring the importance of accurately identifying malaria mosquitoes to understand transmission dynamics and improve vector control. Traditional methods such as morphological identification require extensive training cannot distinguish between sibling species, while molecular approaches are costly for screening. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged rapid...
Background: Amazonian Toxoplasmosis is a recently described form of Toxoplasma gondii infection, characterized by severe clinical and biological features, involvement atypical genetic strains circulating through forest-based cycle. Though mostly reported in French Guiana since 1998, this disease probably under-diagnosed other areas South America. Few data are available on its specific features children. Methods & Materials: We retrospectively included all children seen Cayenne between 2002...