A5002054688- Glioma Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Meningioma and schwannoma management
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Genomics and Chromatin Dynamics
- Ferroptosis and cancer prognosis
- Neurofibromatosis and Schwannoma Cases
- Cancer-related molecular mechanisms research
- Bioinformatics and Genomic Networks
- Pituitary Gland Disorders and Treatments
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Pancreatic and Hepatic Oncology Research
- Brain Metastases and Treatment
- Ubiquitin and proteasome pathways
- Neurogenesis and neuroplasticity mechanisms
- Cancer Immunotherapy and Biomarkers
- Cancer Treatment and Pharmacology
Baylor Scott & White Medical Center - Temple
2024-2025
Baylor College of Medicine
2024-2025
Baylor Medical Center at Garland
2025
University of Louisville
2018-2024
Medical University of Lublin
2023-2024
The Ohio State University Wexner Medical Center
2014-2019
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2014-2018
The Ohio State University
2013-2018
ETH Zurich
2018
SIB Swiss Institute of Bioinformatics
2018
Abstract The objective of this study was to demonstrate 1 H MR spectroscopy (MRS) changes in cerebral metabolites after acute head trauma. Twenty‐five patients (12 children, 13 adults) were examined with quantitative MRS closed injury. Clinical grade (Glasgow Coma Scale [GCS]) and outcome (Rancho Los Amigos Medical Center Outcome Score [ROS]) correlated neurochemical findings. N‐acetylas‐partate (NAA), a neuronal axonal marker, reduced ( P < .03−.001). In NAA/creatine plus phosphocreatine...
The periodic destruction of mitotic cyclins is triggered by the activation anaphase-promoting complex/cyclosome (APC/C) in mitosis. Although ability APC/C to recognize box (D-box) substrates oscillates throughout cell cycle, mechanism regulating binding D-box remains unclear. Here, we show that inhibitor Emi1 tightly binds both and its Cdh1 activator, receptor site on , competes with for binding. itself contains a conserved C-terminal D-box, which provides APC/C-binding affinity,...
Tumor-infiltrating lymphocytes (TIL) have prognostic significance in many cancers, yet their roles glioblastoma not been fully defined. We hypothesized that TILs are associated with molecular alterations, histologies, and survival.
Background and Purpose— Axonal remodeling is critical to brain repair after stroke. The present study investigated axonal outgrowth stroke the signaling pathways mediating in cortical neurons. Methods— Using a rodent model of middle cerebral artery occlusion, we examined high-molecular weight neurofilament (NFH) immunoreactive axons myelin basic protein-positive oligodendrocytes peri-infarct area. In vitro, using cultured neurons microfluidic chamber challenged by oxygen-glucose deprivation...
We characterized the expression and function of endoplasmic reticulum protein GRP78 in glial tumors. is highly expressed glioblastomas but not oligodendrogliomas, its inversely correlated with median patient survival. Overexpression glioma cells decreases caspase 7 activation renders resistant to etoposide- cisplatin-induced apoptosis, whereas silencing cell growth sensitizes etoposide, cisplatin, gamma-radiation. Thus, contributes increased apoptosis resistance may provide a target for...
Astroblastoma (AB) is a rare CNS tumor demonstrating abundant astroblastomatous pseudorosettes. Its molecular features have not been comprehensively studied and its status as entity controversial. We analyzed cohort of 27 histologically-defined ABs using DNA methylation profiling, copy number analysis, FISH site-directed sequencing. Most cases demonstrated mutually exclusive MN1 rearrangements (n = 10) or BRAFV600E mutations 7). Two additional harbored RELA rearrangements. Other lacked these...
Resistance to genotoxic therapies is a primary cause of treatment failure and tumor recurrence. The underlying mechanisms that activate the DNA damage response (DDR) allow cancer cells escape lethal effects remain unclear. Here, we uncover an unexpected mechanism through which pyruvate kinase M2 (PKM2), highly expressed PK isoform in master regulator metabolic reprogramming, integrates with DDR directly promote double-strand break (DSB) repair. In ionizing radiation oxidative stress, ATM...
Engineered oncolytic viruses are used clinically to destroy cancer cells and have the ability boost anticancer immunity. Phosphatase tensin homolog deleted on chromosome 10 loss is common across a broad range of malignancies, implicated in immune escape. The N-terminally extended isoform, phosphatase alpha (PTENα), regulates cellular functions including protein kinase B signaling mitochondrial adenosine triphosphate production. Here we constructed HSV-P10, replicating, PTENα expressing...
Abstract Cancer stem cells (CSCs) are characterized by their self-renewing potential and ability to differentiate phenocopy the original tumor in orthotopic xenografts. Long-term propagation of glioblastoma (GBM) serum-containing medium results loss CSCs outgrowth genetically biologically divergent from parental tumors. In contrast, use a neurosphere assay, serum-free culture for selection, central nervous system-derived allows selection subpopulation containing CSCs. Gliosarcoma (GS),...
Abstract Purpose To retrospectively correlate various diffusion tensor imaging (DTI) metrics in patients with glioblastoma multiforme (GBM) patient survival analysis and also degree of tumor proliferation index determined histologically. Materials Methods Thirty‐four histologically confirmed treatment naive GBMs underwent DTI on a 3.0 Tesla (T) scanner. Region‐of‐interest was placed the whole lesion including enhancing as well nonenhancing component to determine metrics. Kaplan‐Meier...
Abstract Ependymomas originate in posterior fossa ( PF ), supratentorial ST ) or spinal cord SC compartments. At present, grading schemes are applied independent of anatomic site. We performed detailed histological examination on 238 W orld H ealth O rganization grade II and III ependymomas. Among ependymomas, the presence hypercellular areas, necrosis, microvascular proliferation elevated mitotic rate (all P < 0.01) were significantly associated with worse progression‐free survival PFS...
Aurora A is critical for mitosis and overexpressed in several neoplasms. Its overexpression transforms cultured cells, both its knockdown cause genomic instability. In transgenic mice, haploinsufficiency, not overexpression, leads to increased malignant tumor formation. thus appears have tumor-promoting tumor-suppressor functions. Here, we report that protein, measured by quantitative protein gel blotting, differentially expressed major glioma types lineage-specific patterns. levels WHO...
Abstract Background. Astroblastomas (ABs) are rare glial tumors showing overlapping features with astrocytomas, ependymomas, and sometimes other neoplasms, may be challenging to diagnose. Methods. We examined clinical, histopathological, molecular in 28 archival formalin-fixed, paraffin-embedded AB cases performed survival analyses using Cox proportional hazards Kaplan–Meier methods. Results. Unlike ependymomas angiocentric gliomas, ABs demonstrate abundant distinctive astroblastic...
Abstract Papillary tumor of the pineal region (PTPR) is an uncommon with distinctive histopathologic and molecular characteristics. Experience limited respect to its heterogeneity clinical Here, we describe 39 new cases combine these 37 previously published for a cohort 76 PTPR’s, all confirmed by methylation profiling. As reported, two main groups were identified (PTPR-A PTPR-B). In our analysis extended subtyping into three subtypes: PTPR-A, PTPR-B1 PTPR-B2 supported DNA profile genomic...
Abstract Glioblastoma remains a devastating disease for which novel therapies are urgently needed. Here, we report that the Aurora-A kinase inhibitor alisertib exhibits potent efficacy against glioblastoma neurosphere tumor stem–like cells in vitro and vivo. Many treated with short periods undergo apoptosis, although some regain proliferative activity upon drug removal. Extended treatment, however, results complete irreversible loss of cell proliferation. Moreover, caused to partially...
The quality of cancer genomic and proteomic data relies upon the clinical specimens examined. Here, we show that derived from non-microdissected glioblastoma multiforme tumor tissue is either masked or not accurate, producing correlations between lead to false classifications for therapeutic stratification. We analyzed level 133 key signaling proteins phosphoproteins in laser capture microdissected (LCM) primary tumors a study set tissues used Cancer Genome Atlas (TCGA) profiling efforts,...
Brain intraparenchymal schwannoma is a rare clinical entity, generally curable with adequate resection. We describe case in male patient first presenting at 19 months of age, the youngest reported age for this lesion. It also appears to be connected germline TSC2 p.1510del mutation autism-like symptoms. Although tuberous sclerosis not associated increased risk schwannoma, mTORC1 activity, which inhibited by intact TSC1/TSC2 complex, involved progression. This patient's tumor harbored...