A5080176844- Cancer, Hypoxia, and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Probiotics and Fermented Foods
- Immune cells in cancer
- Gut microbiota and health
- Nanoplatforms for cancer theranostics
- Inflammation biomarkers and pathways
- Epigenetics and DNA Methylation
- Microfluidic and Bio-sensing Technologies
- Craniofacial Disorders and Treatments
- Advanced Proteomics Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Liver physiology and pathology
- Protein Tyrosine Phosphatases
- Diabetes and associated disorders
- Inflammasome and immune disorders
- Mechanisms of cancer metastasis
- Metabolism, Diabetes, and Cancer
- Milk Quality and Mastitis in Dairy Cows
- PARP inhibition in cancer therapy
- Melanoma and MAPK Pathways
- Advanced Glycation End Products research
- Biomarkers in Disease Mechanisms
Air Force Medical University
2011-2025
Lanzhou University
2024
Peking University
2024
State Key Laboratory of Natural and Biomimetic Drugs
2024
Southeast University
2019-2024
Chonnam National University
2021-2023
Wayne State University
2023
Icahn School of Medicine at Mount Sinai
2021
Nanjing Maternity and Child Health Care Hospital
2021
Nanjing Medical University
2021
Tumor-associated macrophages (TAMs) are major components of the tumor microenvironment. Although a role for TAMs in promoting progression has been revealed, differentiation mechanisms and intrinsic signals regulated by microenvironment remain unclear. Here we constructed an vitro cell model, TES-TAMs, which is from tumor-extract-stimulated bone-marrow-derived macrophages. We performed comparative proteomics analysis indicated that TES-TAMs possessed characteristic molecular expression TAMs....
PROteolysis TArgeting Chimeras (PROTACs) have been considered the next blockbuster therapies. However, due to their inherent limitations, efficacy of PROTACs is frequently impaired by limited tissue penetration and particularly insufficient cellular internalization into action sites. Herein, based on ultra-pH-sensitive enzyme-sensitive nanotechnology, a type polymer PROTAC conjugated pH/cathepsin B sequential responsive nanoparticles (PSRNs) are deliberately designed, following construction...
Microglial canonical transient receptor potential channel 1 (TRPC1) has been proposed to influence neuroinflammation after cerebral ischemia and reperfusion injury (CIRI), however, the underlying mechanism remains poorly understood. This study demonstrates that TRPC1 is modified by small ubiquitin-related modifier (SUMO)ylation. Our findings suggest a notable increase in microglial SUMOylation within both middle artery occlusion (MCAO/R) model vitro oxygen-glucose deprivation/regeneration...
Liver fibrosis is a worldwide clinical issue. It has been well established that activated hepatic stellate cells (HSCs) are responsible for excessive extracellular matrix (ECM) deposition in chronically damaged livers. The identification of key elements control HSCs activation will help to further our understanding liver and improve the outcome treatment. This study demonstrates N-Myc downstream-regulated gene 2 (NDRG2) potential regulator as NDRG2 mRNA protein levels were reduced during...
Neutrophil infiltration has been linked to worse clinical outcomes after ischemic stroke. Microglia, a key type of immune-competent cell, engage in cross-talk with the infiltrating immune cells inflamed brain area, yet molecular mechanisms involved remain largely unexplored. In this study, we investigated how canonical transient receptor potential 1 (TRPC1) modulated neutrophil male mouse cerebral ischemia and reperfusion injury (CIRI) models. Our findings revealed notable upregulation TRPC1...
Background: Patients with psoriasis have an increased risk of atherosclerotic cardiovascular disease (CVD). The molecular mechanisms behind this connection are not fully understood, but the involvement neutrophils drawn attention as a shared inflammatory factor. Methods: RNA sequencing using Illumina platform was performed on blood from 38 patients moderate to severe psoriasis; approximately half had prior CVD. neutrophil lymphocyte ratio (NLR) obtained samples. Subclinical atherosclerosis...
Neutrophils plays a crucial role in acute ischemic brain injury and have emerged as potential treatment targets to mitigate such injuries. Lysine-specific demethylase 4 A (KDM4A), member of the histone lysine family enzymes involved transcriptional regulation gene expression, is upregulated during hypoxic events. However, exact KDM4A pathological process stroke remains largely unexplored. Our findings reveal that there was an upregulation levels reactive astrocytes within both mouse models...
Reprogrammed metabolism is a new hallmark of cancer. In many types cancer, most the genes in glycolytic pathway are overexpressed, reflecting an essential shift during cancer development. The reprogrammed contributes to development multiple ways, from supplying elevated energy requirement creating microenvironment suitable for tumor growth and suppressing human immune surveillance system.In this study, functional proteomics top-down approach was used systematically monitor metabolic enzyme...
It had been reported that cordycepin could activate AMP‐activated protein kinase. One possible mechanism is mediated kinase activation by conversion into monophosphate, which acts as an AMP analog to To confirm the aforementioned hypothesis, we investigate binding of monophosphate using molecular docking. The modeling results indicate binds with high affinity. hydrogen bonds provide attractive forces between molecules. Our further identify key residues contributing interaction. Also, predict...
This study aimed to investigate the effects of metabolites Latilactobacillus curvatus BYB3 and indole-activated aryl hydrocarbon receptor (AhR) increase tight junction (TJ) proteins in an vitro model intestinal inflammation. In a Western blot assay, L. reduced TJ demage lipoploysaccharide (LPS) stimulated-Caco-2 cells. reduction was result upregulating expression TJ-associated suppressing nuclear factor-κB signaling. Immunofluorescence images consistently revealed that LPS disrupted...
Antibiotic resistance has been considered to be a global threat which underscores the need develop novel anti-infective therapeutics. Modulation of innate immunity by synthetic peptides is an attractive strategy overcome this circumstance. We recently reported that BCCY-1, human β-casein-derived peptide displays regulatory activities on monocytes, thereby enhancing their actions in immune responses. However, function BCCY-1 host defense against infection remains unknown. In study, we...
Energy metabolism may alter pattern differences in acute lung injury (ALI) as one of the causes but detailed features at single-cellular level remain unclear. Changes intercellular temperature and adenosine triphosphate (ATP) concentration within single cell help to understand role energy causing ALI. ALI vitro models were established by treating mice epithelial (MLE-12) cells with lipopolysaccharide (LPS), hydrogen peroxide (H2O2), hydrochloric acid (HCl) cobalt chloride (CoCl2,...
Abstract Designing nanomedicines with low toxicity, high targeting, excellent therapeutic effects, and precise release is always the major challenges in clinical cancer treatment. Here, we report a light‐enhanced tandem‐responsive nano delivery platform COF‐B@X‐03 for amplified anti‐tumor efficiency. Biotin‐loaded could precisely target tumor cells, azo hydrazone bonds it would be depolymerized by overexpressed azoreductase acidic microenvironment hypoxic tumors. In vitro experimental...
Background Gastric cancer (GC) is a highly malignant gastrointestinal tumor characterized by difficult early diagnosis and poor prognosis. Therefore, it imperative to explore potential therapeutic targets for gastric cancer. PARP9 abnormally expressed in variety of tumors associated with cell apoptosis DNA damage. However, its relationship GC has not been fully studied. Methods The expression prognostic significance were examined using bioinformatics approaches. Cell lines either knockdown...
You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology V1 Apr 2017MP98-16 DOWN-REGULATION OF PYRUVATE KINASE 2 BY RNAI AND SMALL MOLECULE INHIBITOR OVERCOMES CISPLATIN RESISTANCE BLADDER CANCER CELLS IN VITRO VIVO Xing Wang, Fenglin Zhang, Herbert Lepor, Moon-shong Tang, Chuanshu Huang, and Xue-Ru Wu WangXing Wang More articles by this author , ZhangFenglin Zhang LeporHerbert Lepor TangMoon-shong Tang HuangChuanshu Huang WuXue-Ru View All Author...
Background and purpose: T cell activation, which is detrimental to the ischemic brain, requires metabolic reprogramming meet increased fuel demanding. ACC1 an enzyme catalyzing carboxylation of acetyl-CoA malonyl CoA, a key substrate in glycolytic-lipogenic pathway, extremely critical for differentiation phenotype polarization. We tested hypothesis that pharmacologically inhibiting early after stroke may restrain activation protect against cerebral injury. Methods: Cerebral ischemia was...
Objective To explore the correlations between circulating plasmacytoid dendritic cells (pDCs) and other immune cells, clinical parameters in patients with type 1 diabetes mellitus (T1DM), to investigate role of pDCs pathogenesis T1DM. Methods A total 62 T1DM who visited endocrinology at First Affiliated Hospital Nanjing Medical University from December 2015 June 2017 were recruited as study group. A 74 age gender matched healthy volunteers control Clinical all subjects including age,...