A5101935730- Circular RNAs in diseases
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Radiomics and Machine Learning in Medical Imaging
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- Colorectal Cancer Treatments and Studies
- Lung Cancer Research Studies
- Esophageal Cancer Research and Treatment
- Lung Cancer Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- Gut microbiota and health
- TGF-β signaling in diseases
- Cell Adhesion Molecules Research
- AI in cancer detection
- Pancreatic and Hepatic Oncology Research
- Cancer, Lipids, and Metabolism
- Colorectal Cancer Screening and Detection
- Cancer Mechanisms and Therapy
Jiangsu Cancer Hospital
2019-2024
Nanjing Medical University
2019-2024
Soochow University
2020-2023
First Affiliated Hospital of Soochow University
2020-2023
The Fourth Affiliated Hospital of Soochow University (Suzhou Dushu Lake Hospital)
2022
Second Affiliated Hospital of Nanjing Medical University
2020
National Taiwan University Hospital
2009
Aberrant expression of circular RNAs (circRNAs) is involved in cancer progression through interaction with RNA-binding proteins (RBPs). Herein, we screened circRNA A549 cells circBase and the crosslinking immunoprecipitation (CLIP) data human antigen R (HuR), an extensively studied RBP, identified a circRNA, circ-defective cullin neddylation 1 domain containing 4 (circDCUN1D4), originating from DCUN1D4 gene transcript. circDCUN1D4 downregulated tumor samples under mediation DExH-box helicase...
Abstract Background Lung adenocarcinoma (LUAD) is the leading cause of death worldwide. However, roles long noncoding RNAs (lncRNAs) hijacked by super-enhancers (SEs), vital regulatory elements epigenome, remain elusive in progression LUAD metastasis. Methods SE-associated lncRNA microarrays were used to identify dysregulated lncRNAs LUAD. ChIP-seq, Hi-C data analysis, and luciferase reporter assays utilized confirm hijacking LINC01977 SE. The functions mechanisms explored a series vitro...
Abstract In recent years, circular RNA s (circ s) have been revealed to important roles in carcinogenesis. Metastasis is the leading cause of lung adenocarcinoma ( LUAC ) death. However, contributions circ metastasis remain largely unknown. Based on circBase data and our biobank tissues, we identified CRIM 1 (a derived from exons 2, 3 4 gene, hsa_circ_0002346) as having a significantly decreased expression samples compared with matched normal control samples. Both vivo vitro experiments that...
Collagens are major components of the ECM in various organs, including lungs. Ectopic expression collagens can regulate tumor progression and disease outcome through remodeling extracellular matrix (ECM). However, it remains largely unexplored whether involved lung adenocarcinoma (LUAD). Analysis three LUAD transcriptional profiles showed that COL10A1 mRNA was up-regulated associated with poor prognosis. Gain- loss-of-function studies were performed to observe promotes cell proliferation...
Abstract Tertiary lymphoid structures (TLSs) are considered to have a good prognosis in multiple solid tumors. However, the prognostic value of TLS esophageal squamous cell carcinoma (ESCC) is unknown. In this study, we retrospectively enrolled 185 ESCC patients who underwent surgical resection. Hematoxylin and eosin staining was performed investigate presence, abundance, maturation, location TLSs. We explored cellular composition TLSs using traditional immunohistochemistry serial sections....
Background It has been reported that smoking history as a predictor of immunotherapy efficacy in patients with advanced lung cancer, however, the underlying mechanisms this phenomenon remain largely unknown. Methods The adenocarcinoma’s (LUAD) cohort and orthotopical transplanted mouse model were used to explore correlation between status tertiary lymphoid structure (TLS) chemokine CCL21, respectively. Cell adhesion co-immunoprecipitation assays performed interaction CD4+T cells CD20+B under...
Classification of histological patterns in lung adenocarcinoma (LUAD) is critical for clinical decision-making, especially the early stage. However, inter- and intraobserver subjectivity pathologists make quantification varied inconsistent. Moreover, spatial information not evident to naked eye pathologists.We establish LUAD-subtype deep learning model (LSDLM) with optimal ResNet34 followed by a four-layer Neural Network classifier, based on 40 000 well-annotated path-level tiles. The LSDLM...
Abstract The proposed competing endogenous RNA (ceRNA) mechanism suggested that diverse species, including protein-coding messenger RNAs and non-coding such as long RNAs, pseudogenes circular could communicate with each other by for binding to shared microRNAs. ceRNA network (ceRNET) is involved in tumor progression has become a hot research topic recent years. To date, more attention been paid the role of crosstalk. However, coding transcripts are abundant powerful than make up majority...
Osimertinib (Osi) is a widely used epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI). However, the emergence of resistance inevitable, partly due to gradual evolution adaptive resistant cells during initial treatment. Here, we find that Osi treatment rapidly triggers in tumor cells. Metabolomics analysis reveals significant enhancement oxidative phosphorylation (OXPHOS) adaptive-resistant Mechanically, induces an elevation NCOA4, key protein ferritinophagy, which...
Abstract Mutation or downregulation of p53 (encoded by TP53) accelerates tumorigenesis and malignant progression in esophageal squamous cell carcinoma (ESCC). However, it is still unknown whether circular RNAs (circRNAs), a novel class endogenous noncoding RNAs, participate the regulation this progress. In study, we explored expression profiles circRNAs three paired samples ESCC identified cCNTNAP3, which circRNA that originates from CNTNAP3 gene transcript highly expressed normal human...
Recent evidence demonstrates the existence of diversified microbiota in lung. However, effect lung carcinogenesis on flora microenvironment has yet not been well investigated. In this study, we surveyed microbial composition and diversity tumor paired adjacent normal tissues obtained from 55 cancer patients to test whether any specific tumor-associated features can be identified. Compared with non-malignant tissues, samples showed significantly lower community richness (α diversity), but no...
Lung cancer is the leading cause of related to mortality worldwide, and main pathological type lung adenocarcinoma (LUAD). Circular RNAs (circRNAs) have been reported be modified by N6 -methyladenosine (m6A), which involved in progression diverse tumors. However, crosstalk between circRNAs m6A modification has not well elucidated LUAD.MeRIP-seq YTHDF2-RIP-seq datasets were explored identify candidate YTHDF2. Dual-luciferase reporter assay, RIP, rescue assays performed explore relationship...
This study aimed to develop a reliable immune signature based on B-cell proportion predict the prognosis and benefit of immunotherapy in LUAD. The cells TCGA-LUAD dataset was estimated using MCP-counter. Least Absolute Shrinkage Selector Operation used identify prognostic validated an independent cohort. We quantitative reverse transcription-polymerase chain reaction (qRT-PCR) data formalin-fixed paraffin-embedded (FFPE) specimens immunohistochemistry illustrate correlation between leukocyte...
Abstract Lung squamous cell carcinoma (LUSC) is a histological subtype of non-small lung cancer with the worse progression. SRY-Box Transcription Factor 2 (SOX2) copy number amplification (CNA) oncogenic driver in ~60% patients diagnosed LUSC. Thus, SOX2 represents an effective therapeutic target -amplified However, protein was considered undruggable. Here, we report expression circular RNA, cicSOX2 Patients -CAN LUSC expressing circSOX2 manifested increased survival outcomes. CircSOX2...
The profile of immune-related adverse events (irAEs) due to programmed death-1 (PD-1) inhibitors-based combination therapy in advanced non-small cell lung cancer (NSCLC) and its relationship with survival have not been fully described.Designed capture the spectrum irAEs explore association between clinical outcomes patients NSCLC.This retrospective single-center study included NSCLC treated PD-1 inhibitors (mainly chemotherapy) at Jiangsu Cancer Hospital.The was explored using landmark...
Lung adenocarcinoma (LUAD) exhibits high heterogeneity and is well known for its genetic variation. Recently, the understanding of non-genetic variation provides a new perspective to study LUAD. Little about whether super-enhancers (SEs) may be primarily responsible inter-tumor We used super-enhancer RNA (seRNA) levels large-scale clinical well-annotated LUAD cohort stratify patients into three clusters with different prognosis other malignant characteristics. Mechanistically,...
We performed a bioinformatics analysis to screen for cell cycle-related differentially expressed genes (DEGs) and constructed model the prognostic prediction of patients with early-stage lung squamous carcinoma (LSCC). From gene expression omnibus (GEO) database, GSE157011 dataset was randomly divided into an internal training group testing at 1:1 ratio, GSE30219, GSE37745, GSE42127, GSE73403 datasets were merged as external validation group. single-sample set enrichment (ssGSEA), univariate...
An inverted AluYb8 insertion in the MUTYH intron 15 (AluYb8MUTYH variant) has been reported to be associated with reduced MUTYH1 expression and mitochondrial dysfunction age. However, underlying mechanism remains unknown. In this study, we identified a novel transcript AluYb8MUTYH variant, which revealed that is about 780 nucleotides length poly-A tail, lacks protein-coding potential, referred as lncMUTYH. The results from reporter gene system confirmed lncMUTYH down-regulates at...
Lung cancer is associated with the greatest number of cancer-related deaths worldwide. adenocarcinoma (LUAD) accounts for 85% all cases lung cancer. Despite recent advances in treatment, 5-year survival rate remains less than 15%. Thus, diagnostic and therapeutic role LUAD remain to be further studied. The prolyl 3-hydroxylase family member 4 (P3H4) involved various cancers, but little known about its LUAD. Our study demonstrated that P3H4 gene was upregulated Clinically, expression...