A5018388754- RNA modifications and cancer
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Reproductive Biology and Fertility
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Intracranial Aneurysms: Treatment and Complications
- Virus-based gene therapy research
- CAR-T cell therapy research
- Chemokine receptors and signaling
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Advanced biosensing and bioanalysis techniques
- Genomics, phytochemicals, and oxidative stress
- Protein Hydrolysis and Bioactive Peptides
- Tryptophan and brain disorders
- Cancer Immunotherapy and Biomarkers
- Pharmacological Receptor Mechanisms and Effects
- Cancer-related Molecular Pathways
- Selenium in Biological Systems
Anhui Medical University
2023-2025
Nanjing Medical University
2024-2025
Guangdong Academy of Medical Sciences
2024
Southern Medical University
2024
Guangdong Provincial People's Hospital
2024
Changsha Medical University
1995-2023
First Affiliated Hospital of Chengdu Medical College
2020-2022
First Affiliated Hospital of Wannan Medical College
2020-2022
Wannan Medical College
2021-2022
Wuzhou University
2022
Abstract Cervical cancer is the second leading cause of morbidity and mortality in women worldwide. Traditional treatment methods have become limited. Naringenin, a flavonoid abundant various fruits herbal medicines, has demonstrated anti-tumor properties among other effects. This research undertook to elucidate mechanism naringenin context cervical by leveraging network pharmacology performing experimental validation. Initial steps involved predicting potential targets subsequently...
As the chemical environment of semen can have a profound effect on sperm quality, we examined pH motility, viability and capacitation human sperm. The in this study was collected from healthy males to avoid interference other factors. spermatozoa cultured nutrition solution at 5.2, 6.2, 7.2 8.2 were analyzed for total progressive motility (PR), hypo-osmotic swelling (HOS) rate, penetration. Our results showed that these parameters similar solutions, but decreased 5.2 6.2 solutions. HOS rate...
Nasopharyngeal carcinoma (NPC) is a cancer with high metastatic rate and poor prognosis. Growing studies suggest that ferroptosis take part in the development of tumours. At same time, connection between ferroptosis-related genes (FRGs) prognosis NPC remains unclear. In this study, we explored dysregulated FRGs normal control tumour samples NPC. Firstly, 14 36 differentially expressed were identified tissues compared to tissues, among which ABCC1, GLS2, CS HMGCR associated for patients. The...
Nuclear factor erythroid 2-related 2 (Nrf2) is capable of inducing a variety biological effects, and the regulation Nrf2 signaling pathway closely related to longevity. To find out whether nuclear involved in oocyte aging or not which may cause reduced female fertility, series methods was applied, including collection culture, micro injection, RNA interference, western blotting, immunofluorescence confocal microscopy, quantitative real-time PCR.Our data demonstrated that depletion disrupted...
Linc00312 is downregulated in nasopharyngeal carcinoma (NPC) and associates with poor treatment efficacy. Genetic variations are the main cause of individual differences response. The objective this study to explore relationship between genetic linc00312 risk chemoradiotherapy induced toxic reactions NPC patients. We used a bioinformatics approach select 3 single nucleotide polymorphisms (SNPs) regulatory feature (rs12497104, rs15734, rs164966). 505 patients receiving complete follow-up data...
The primary challenges in CD19-specific chimeric antigen receptor T-cell (CD19 CAR T) therapy for patients with refractory/relapsed B-cell acute lymphoblastic leukemia (r/r B-ALL) are non-response and relapse; it is urgent to reveal these mechanisms. Neutrophils play a critical role the immunosuppressive tumor microenvironment (TME), which can hinder T efficacy. Our previous research identified subset of neutrophils special phenotype (CD14-CD10-CD45-HLA-DR-SSC++, termed CD10- neuts),...
Abstract N‐Doped TiO 2 photocatalysts were prepared by a hydrothermal method with tetra‐ n ‐butyl titanate (TTNB) and triethanolamine as precursors. The obtained samples characterized X‐ray diffraction (XRD), transmission electron microscopy (TEM), UV‐visible diffuse reflectance spectra (DRS), respectively. Photocatalytic activities of the anatase products investigated on degradation methyl orange (MO). incorporation nitrogen impurity in was studied first‐principles calculations based...
Transcription factor AP-2 alpha (AP-2α or TFAP2A) is a newly identified prognostic marker of chemotherapy; its expression positively correlated with chemosensitivity and survival cancer patients.Using computational programs, we predicted that the coding region AP-2α gene contains potential miRNA response element (MRE) miR-193a-5p, single nucleotide polymorphism (SNP) site (c.497A>G, rs111681798) resides within MRE.The results luciferase assays Western blot analysis demonstrated miR-193a-5p...
The Sonic Hedgehog (Shh) signaling pathway is commonly activated in gastrointestinal cancer. However, our understanding of the Shh gastric cancer remains limited. Here we examined effects cyclopamine, a specific inhibitor pathway, on cell growth and proliferation primary cells GAM-016 MKN-45 line. results showed that molecules SHH, PTCH, SMO, GLI1, GLI2 were intact both types cells. Furthermore, observed cyclopamine inhibited through cycle arrest apoptosis. An vivo study using NOD/SCID mouse...
Atherosclerosis is a chronic inflammatory disease with high prevalence and mortality. The rupture of atherosclerotic plaque the main reason for clinical events caused by atherosclerosis. Making clear transcriptomic proteomic profiles between stabe unstable plaques crucial to prevent manifestations. In present study, 5 stable human carotid were obtained endarterectomy. samples used whole transcriptome sequencing (RNA-Seq) Next-Generation Sequencing using Illumina HiSeq, proteome analysis...
Gynura procumbens is a traditional herb used for the treatment of inflammation, rheumatism and viral infections, although antitumor effect its potential mechanisms action remain unclear. In present study, ethanolic extract (GPE) on osteosarcoma (OS) cell line, U2-OS, was investigated in vitro. Cell proliferation apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) flow cytometry assays, respectively. Transwell invasion wound healing assays performed...
Brain-derived neurotrophic factor (BDNF) and FSH receptor (FSHR) are expressed in ovarian granulosa cells, play important roles regulating follicle growth oocyte maturation. Studies have linked the BDNF-associated signaling pathway to FSHR mRNA expression regulation of development, but mechanisms remain unknown. In current study, we found that BDNF stimulated secretion estradiol progesterone, increased proliferation KGN cells (human granulosa-like tumor cell line). treatment also...
Blebs are rupture risk factors in intracranial aneurysms (IAs), but their prevalence, distribution, and associations with clinical as well causes effects on aneurysm vulnerability remain unclear.A total of 122 blebs 270 IAs selected for surgery were studied using patient-specific vascular reconstructions from 3D angiographic images. Bleb geometry, location the aneurysm, frequency occurrence at different locations analyzed. Associations between gender, age, smoking, hypertension, hormone...
Abstract Background Drug resistance is a major cause of therapeutic failure that often associated with elevated autophagy and apurinic/apyrimidinic endonuclease 1 (APE1) expression. Herein, we investigated the role APE1 in A549 cells treated cisplatin. Methods SILAC proteomics was applied to obtain panoramic view cisplatin treatment KRAS G12S -mutant cells. Quantity analysis cellular apoptosis based on flow cytometry. Western blotting used examine expression levels apoptosis-...
SOX2 is related to drug resistance in many types of cancer, including lung cancer. Herein, we investigated the role and its regulatory signaling cisplatin-treated non-small-cell cancer (NSCLC). The effects on cell viability, proliferation, apoptosis were evaluated vitro. Western blotting, real-time quantitative PCR, immunohistochemistry, luciferase reporter assays used investigate underlying mechanism. Kaplan-Meier survival analysis log-rank test assess relationship between expression...
PA28γ (also called REGγ, 11Sγ or PSME3) negatively regulates p53 activity by promoting its nuclear export and/or degradation. Here, using the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) method, we identified transcription start site gene. Assessment with luciferase assay demonstrated that sequence −193 to +16 is basal promoter. Three binding sites were found within promoter utilizing a bioinformatics approach and confirmed chromatin immunoprecipitation biotinylated DNA...
This study aimed to elucidate the effect of electroacupuncture treatment on preventing early ovarian hyperstimulation syndrome (OHSS) and potential mechanisms involved using an induced rat model. The response was examined by measuring ovary weight, vascular permeability, levels inflammation (interleukin-6), tumour necrosis factor alpha, chemokine ligand 2 (also known as monocyte chemoactic protein 1), endothelial growth hormone concentrations (oestradiol, progesterone, testosterone...
Colorectal cancer (CRC) is one of the most common types and a leading cause cancer-associated mortality in China. Increased thioredoxin reductase 1 (TrxR1) levels have been previously identified as possible target for CRC. The present study revealed that natural product hydroxytyrosol (HT), which exhibits polyphenol scaffold, potent inhibitor TrxR1. Inhibition TrxR1 was indicated to result accumulation reactive oxygen species, inhibit proliferation induce apoptosis G1/S cell cycle arrest CRC...