A5007153087- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Colorectal Cancer Screening and Detection
- Oral microbiology and periodontitis research
- RNA modifications and cancer
- Genetic Mapping and Diversity in Plants and Animals
- Gastric Cancer Management and Outcomes
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Galectins and Cancer Biology
- Metabolomics and Mass Spectrometry Studies
- Global Cancer Incidence and Screening
- Esophageal Cancer Research and Treatment
- Rice Cultivation and Yield Improvement
- Head and Neck Cancer Studies
- Microscopic Colitis
- Pelvic floor disorders treatments
- Probiotics and Fermented Foods
- Nutritional Studies and Diet
- Food composition and properties
- Viral-associated cancers and disorders
- Ginseng Biological Effects and Applications
- Oral health in cancer treatment
- Urinary Tract Infections Management
- Chronic Obstructive Pulmonary Disease (COPD) Research
National Cancer Institute
2012-2022
Division of Cancer Epidemiology and Genetics
2013-2022
Center for Scientific Review
2022
Nanjing Medical University
2019-2020
University of Kansas Medical Center
2019-2020
Nanjing Jiangning Hospital
2020
Jiangsu Province Hospital
2019
National Institutes of Health
2012-2017
United States Department of Health and Human Services
2016-2017
Leidos (United States)
2015
We investigated whether the gut microbiota differed in 48 postmenopausal breast cancer case patients, pretreatment, vs control patients. Microbiota profiles fecal DNA were determined by Illumina sequencing and taxonomy of 16S rRNA genes. Estrogens quantified urine. Case-control comparisons employed linear unconditional logistic regression α-diversity (PD_whole tree) UniFrac analysis β-diversity, with two-sided statistical tests. Total estrogens correlated patients (Spearman Rho = 0.37, P...
The human lung tissue microbiota remains largely uncharacterized, although a number of studies based on airway samples suggest the existence viable microbiota. Here we characterized taxonomic and derived functional profiles in 165 non-malignant from cancer patients. We show that is distinct microbial communities oral, nasal, stool, skin, vagina, with Proteobacteria as dominant phylum (60 %). Microbiota alpha diversity increases environmental exposures, such air particulates, residence low to...
The goal of the study was to investigate whether cigarette smoking alters oral and nasal microbial diversity, composition, structure. Twenty-three current smokers 20 never were recruited. From each subject, nine samples including supra subgingiva plaque scrapes, saliva, swabs from five soft tissue sites, one swab both anterior nares collected. 16S rRNA V3-V4 region sequenced for profiles. We found that alpha diversity lower in than nonsmokers buccal mucosa, but other sample composition not...
Helicobacter pylori (Hp) is the primary cause of gastric cancer but we know little its relative abundance and other microbes in stomach, especially at time diagnosis. Here characterized taxonomic derived functional profiles microbiota two different sets patients, compared them with microbial body sites. Paired nonmalignant tumor tissues were sampled from 160 patients 80 China Mexico. The 16S rRNA gene V3-V4 region was sequenced using MiSeq platform for profiles. PICRUSt used to predict Human...
The human upper digestive tract microbial community (microbiota) is not well characterized and few studies have explored how it relates to health. We examined the relationship between microbiota two cancer-predisposing states, serum pepsinogen I/pepsinogen II ratio (PGI/II; predictor of gastric cancer risk) esophageal squamous dysplasia (ESD; precursor lesion cell carcinoma; ESCC) in a cross-sectional design.The Human Oral Microbe Identification Microarray was used test for presence 272...
Different bacteria in stool have markedly varied growth and survival when stored at ambient temperature. It is paramount to develop optimal biostabilization of samples during collection assess long-term storage for clinical specimens epidemiological microbiome studies. We evaluated the effect media delayed freezing up 7 days on microbial composition. Ten participants collected triplicate each into no as well RNAlater® with without kanamycin or ciprofloxacin. For set conditions, were frozen...
Abstract Background Previous studies suggested associations between the oral microbiome and lung cancer, but were predominantly cross-sectional underpowered. Methods Using a case-cohort design, 1306 incident cancer cases identified in Agricultural Health Study; National Institutes of Health-AARP Diet Prostate, Lung, Colorectal, Ovarian Cancer Screening Trial. Referent subcohorts randomly selected by strata age, sex, smoking history. DNA was extracted from wash specimens using DSP Virus...
Background Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in fields human health and environmental sciences. This approach, however, is difficult when amount DNA too low be amplified by standard PCR. Nested PCR can employed as it amplify samples with concentration several-fold lower than However, potential biases nested PCRs that could affect measurement structure have received little attention. Results In this study, we used...
Cesarean birth is associated with altered composition of the neonate's microbiota and increased risk for obesity other diseases later in life. The mechanisms these associations, whether cesarean an adult microbiota, are unknown.In 1097 volunteers without diabetes, inflammatory bowel disease, or recent antibiotic use, fecal microbiome metrics were compared by history (N=92) appendectomy (N=115). Associations potential confounders, alpha diversity, individual microbial taxa estimated logistic...
Because differences in anal microbial populations (microbiota) could affect acquisition of HIV or other conditions, especially among MSM, we profiled the microbiota canal, assessed its stability, and investigated associations with diversity composition.Microbiota profiles swabs collected from 76 MSM (52 1989, swab-1; 66 1-5 years later, swab-2) were compared by status (25 HIV-positive), T-cell subsets, questionnaire data.Bacterial 16S rRNA genes amplified, sequenced (Illumina MiSeq),...
The microbiome is the collection of all microbial genes and can be investigated by sequencing highly variable regions 16S ribosomal RNA (rRNA) genes. Evidence suggests that environmental factors host genetics may interact to impact human composition. Identifying genetic variants associated with composition not only provides clues for characterizing variation but also helps elucidate biological mechanisms associations, prioritize variants, improve risk prediction. Since a microbiota functions...
Screening for colorectal cancer (CRC) and precancerous adenoma (CRA) can detect curable disease. However, participation in colonoscopy sensitivity of fecal heme CRA are low.Microbiota metrics were determined by Illumina sequencing 16S rRNA genes amplified from DNA extracted feces self-collected RNAlater. Among immunochemical test-positive (FIT +) participants, colonoscopically-defined normal versus patients compared regression, permutation, random forest plus leave-one-out methods.Of 95 FIT...
Genetic susceptibility is likely involved in nasopharyngeal carcinoma (NPC), a cancer caused by Epstein-Barr virus (EBV) infection. Understanding of genetic factors NPC and how they contribute to EBV-induced carcinogenesis limited. We conducted whole-exome capture/sequencing among 251 individuals from 97 multiplex families Taiwan (205 affected, 21 obligate carriers, 25 unaffected) using SeqCap EZ Human Exome Library v3.0 Illumina HiSeq. Aligned sequences were filtered identify...
Recent studies have revealed significant roles of neurotransmitters and gut microbiota along the gut–brain axis in Parkinson's disease (PD); however, potential mechanisms remain poorly understood. In current study, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced characteristic PD neurobehavior changes accompanied by increased α-synuclein, apoptotic protein Bim, cleaved caspase-3 decreased expression tyrosine hydroxylase (TH). Meanwhile, tryptophan (Trp) (Tyr) neurotransmitter...
Objective Studies of the fecal microbiome have implicated gut microbiota in obesity, but few studies examined microbial diversity at other sites. The association between obesity and upper gastrointestinal (UGI) was explored. Methods UGI 659 healthy Chinese adults with a measured body mass index (BMI) range 15.0 to 35.7 characterized using 16S rRNA gene DNA microarray (HOMIM). Results In multivariate‐adjusted models, alpha not associated BMI. However, beta diversity, assessed by principal...
Little is known about the link between gastric microbiota and epidemiology of cancer. In order to determine epidemiologic clinical relevance microbiota, we used 16 S ribosomal RNA gene sequencing analysis characterize composition structure microbial community 80 paired samples (non‐malignant matched tumor tissues) from cardia adenocarcinoma (GCA) patients in Shanxi, China. We also PICRUSt predict functional profiles. Compared without family history upper gastrointestinal (UGI) cancer...
Helicobacter pylori (H. pylori) seropositivity has been inconsistently associated with pancreatic cancer. We, therefore, investigated the association between H. and cancer in a case-control study nested within Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study (ATBC) cohort of Finnish male smokers. Pancreatic cases (n = 353) control subjects were matched on date baseline serum collection, age at randomization, follow-up time (up to 23.9 years). We used multiplex serology assay determine...
Abstract Background: The human microbiota is postulated to affect cancer risk, but collecting specimens with prospective follow-up for diseases will take time. Buccal cell samples have been obtained from mouthwash the study of genomic DNA in many cohort studies. Here, we evaluate feasibility using buccal examine associations and disease risk. Methods: We cells 41 healthy participants a protocol that widely employed obtain DNA. compared oral eight other sample types collected by following...
The origin and evolution of "ORFans" (suspected genes without known relatives) remain unclear. Here, we take advantage a unique opportunity to examine the population diversity thousands ORFans, based on collection 35 complete genomes isolates Escherichia coli Shigella (which is included phylogenetically within E. coli). As expected from previous studies, ORFans are shorter AT-richer in sequence than non-ORFans. We find that often very narrowly distributed: most common pattern for an ORFan be...