A5100426102- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Machine Learning in Bioinformatics
- Chromosomal and Genetic Variations
- Circular RNAs in diseases
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Genetics and Plant Breeding
- Testicular diseases and treatments
- Heavy Metal Exposure and Toxicity
- Antimicrobial Peptides and Activities
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Photosynthetic Processes and Mechanisms
- Dietary Effects on Health
- Genetic Syndromes and Imprinting
- Circadian rhythm and melatonin
Zhejiang Lab
2024
Nanjing Medical University
2024
Yuhuangding Hospital
2023
Qingdao University
2023
Northwest A&F University
2017-2022
Harbin Medical University
2022
Second Affiliated Hospital of Harbin Medical University
2022
Qiqihar Medical University
2022
Sichuan University
2022
Tianjin Beichen Hospital
2022
Abstract Spermatogenesis, an intricate process occurring in the testis, is responsible for ongoing production of spermatozoa and thus cornerstone lifelong male fertility. In spermatogenesis occurs optimally at a temperature 2–4°C lower than that core body. Increased scrotal generates testicular heat stress later causes atrophy spermatogenic arrest, resulting sperm yield therefore impaired Melatonin (N-acetyl-5-methoxytryptamine), small neuro-hormone synthesized secreted by pineal gland...
DNA methylation and histone modifications critically regulate the expression of many genes repeat regions during spermatogenesis. However, molecular details these processes in male germ cells remain to be addressed. Here, using isolated murine sperm cells, ultra-low–input native ChIP-Seq (ULI-NChIP-Seq), whole genome bisulfite sequencing (WGBS), we investigated genome-wide patterns 3 Lys-9 trimethylation (H3K9me3) mouse We found that H3K9me3 have distinct sequence preferences dynamics...
Spermatogenesis carries the task of precise intergenerational transmission genetic information from paternal genome and involves complex developmental processes regulated by testicular microenvironment. Studies performed mainly in mouse models have established theoretical basis for spermatogenesis, yet wide interspecies differences preclude direct translation findings, farm animal studies are progressing slowly. More than 32,000 cells prepubertal (3-month-old) pubertal (24-month-old) buffalo...
The number of teats is related to the nursing ability sows. In present study, we conducted genome-wide association studies (GWAS) for traits teat in Duroc pig population. Two mixed models, one counted and another binary phenotypic traits, were employed analyze seven traits: right (RTN), left (LTN), total (TTN) numbers; maximum on a side (MAX); minus (LR); absolute value LR (ALR); presence symmetry between numbers (SLR). We identified 11, 1, 4, 13, 9 significant SNPs associated with RTN, LTN,...
Spermatogonial stem cells (SSCs) are able to undergo both self-renewal and differentiation. Unlike self-renewal, which replenishes the SSC progenitor pool, differentiation is an irreversible process committing meiosis. Although preparations for meiotic events in differentiating spermatogonia (Di-SG) likely be accompanied by alterations chromatin structure, three-dimensional architectural differences between SSCs Di-SG, higher-order dynamics during spermatogonial differentiation, have not...
Insect defensins are effector components of the innate defense system. During infection, these peptides may play a role in control pathogens by providing protective antimicrobial barriers between epithelial cells and hemocoel. The cDNAs encoding four migratory locust, Locusta migratoria, designated LmDEF 1, 3-5, were identified for first time transcriptome-targeted analysis. Three members this CSαβ defensin family, 3, 5, detected locust tissues. pro regions their sequences have little-shared...
Spermatogonial stem cells (SSCs) are capable of both self-renewal and differentiation to mature functional spermatozoa, being the only adult in males that can transmit genetic information next generation. Porcine SSCs hold great value transgenic pig production establishment porcine models for regenerative medicine. However, studies applications have been greatly hampered by low number testis as well lack an ideal stable long-term culture system propagate perpetually.In present study,...
Summary Cryptorchidism, characterized by the presence of one (unilateral) or both (bilateral) undescended testes, is a common male urogenital defect. Cryptorchidism can lead to infertility, testicular cancer being most extreme clinical symptom, as well psychological issues inflicted individual. Despite this, knowledge about aetiology cryptorchidism and mechanism for cryptorchidism-induced infertility remain limited. In this present study, using an artificial cryptorchid mouse model, we...
Spermatogonial stem cells (SSCs), as tissue-specific cells, are capable of both self-renewal and differentiation supporting the continual robust spermatogenesis for male fertility. As a rare sub-fraction undifferentiated spermatogonia, SSCs share most molecular markers with progenitor spermatogonia. Thus, heterogeneity often obscures characteristics cells. Distinguishing from progenitors is paramount importance to understand regulatory mechanisms governing their actions.The present study was...
Abstract Background Sertoli cells (SCs) create a specialized environment to support and dictate spermatogenesis. MicroRNAs (miRNAs), kind of ~ 22 nt small noncoding RNAs, have been reported be highly abundant in mouse SCs play critical roles However, the miRNAs porcine remain largely unknown. Methods We isolated conducted RNA sequencing. By comparing germ cells, we systematically analyzed miRNA expression pattern SCs. screened enriched SC predicted their functions by Gene Ontology analysis....
Abstract Dithiocarbamate has been tested for its effective anti-tumor activity, but the underlying mechanism remains unclear. We previously prepared a novel diththiocarbamate derivative, DpdtC with an ability of catalase inhibition. Here, we first time investigated growth inhibition effects on HER2-amplified cancer cells and elucidated action. Results showed that exerted potent against HER2-overexpressed SK-OV-3 SK-BR-3 cells, especially higher NDRG1 level, which was also confirmed in...
Spermatogenesis is a continual process that occurs in the testes, which diploid spermatogonial stem cells (SSCs) differentiate and generate haploid spermatozoa. This highly efficient intricate orchestrated at multiple levels. N
RBM10 is the RNA-binding protein often absent or mutated in lung adenocarcinoma, rendering it as a potential biomarker even therapeutic target to prolongate survival time. In this study, we investigated involvement of mutation pathogenesis and tumorigenesis adenocarcinoma identified differentials relative signal pathways, aiming provide new approaches. By performing systematic TCGA analysis, our results demonstrated that was 6% patients, meanwhile 113 functional genes were significant...
SETDB1, a histone H3 lysine 9 (H3K9) methyltransferase, is indispensable in the meiosis and embryo development. Meanwhile, reactive oxygen species (ROS) play critical roles SSC self-renewal. This study aimed to investigate SETDB1 whether associate with homeostasis. We found that knockdown of led increase level through NADPH oxidase, impaired cell proliferation. deficiency also activated ROS downstream signaling pathways including JNK p38 MAPK, which possibly contributed apoptosis. Rescue...
Lung squamous cell carcinoma (LUSC) is the main cause of cancer-related mortality. Some studies demonstrate that m6a and long noncoding RNA (lncRNA) are vital in pathogenesis LUSC. In this study, we aimed to further understand prognostic value m6a-related lncRNAs LUSC their role immune microenvironment. For this, obtained transcriptome clinical data from TCGA database. Further, identified prognostically relevant were clustered into groups based on lncRNA expression. Further analysis...
Background: N7-methylguanosine (m7G) modification plays a crucial role in the development and progression of lung cancers. MicroRNAs (miRNAs) are closely involved programmed cell death mechanism tumor growth. The m7G-associated miRNAs genes adenocarcinoma (LUAD), their prognosis prediction ability LUAD, however, had not been investigated. Methods: RNA transcriptomes, clinical indices, immune scores LUAD patients were searched downloaded from Cancer Genome Atlas (TCGA) ESTIMATE database....
Nasopharyngeal carcinoma (NPC) is a commonly occurring tumor in southern China and Southeast Asia. The current study focused on developing an extensive analysis method for the peripheral integral proteins of NPC cell line HNE1. membrane were extracted by biotinylated enrichment, 0.1 M Na2CO3, H2O. Integral or total plasma fractions prepared using 30% Percoll density grade centrifugation with without Na2CO3 treatment evaluated Western blot analysis. subjected to two-dimensional...
Circular RNA is emerging functional molecule for glioblastoma. However, the function and regulatory of circular (circRNA) remains unclear. In this study, circRNA sequencing array data glioblastoma were analyzed by multiple bioinformatics methods to establish a potential molecular sponge mechanism regulation network.Gene Expression Omnibus datasets used extract circRNAs. CircInteractome was predict microRNAs binding Chinese Glioma Gene Atlas database screen with expression survival trends....
ABSTRACT Spermatogenesis is a highly unique and intricate process, finely regulated at multiple levels, including post‐transcriptional regulation. N6‐methyladenosine (m6A), the most prevalent internal modification in eukaryotic mRNA, plays significant role transcriptional regulation during spermatogenesis. Previous research indicated extensive m6A each stage of spermatogenesis, but depletion Mettl3 and/or Mettl14 spermatogenic cells with Stra8‐Cre did not reveal any detectable abnormalities...
ABSTRACT The overactivation of transposable elements (TEs) is a significant threat to male reproduction, particularly during the delicate process spermatogenesis. Here, we report that zinc finger protein ZCCHC8—a key component nuclear exosome targeting (NEXT) complex involved in ribonucleic acid (RNA) surveillance—is required for TE silencing Loss ZCCHC8 results delayed meiotic progression and reduced production round spermatids (RS). We observed young long-interspersed element (LINE1, L1)...