A5102023052- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Mast cells and histamine
- Immune cells in cancer
- MicroRNA in disease regulation
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Colorectal Cancer Surgical Treatments
- Diverticular Disease and Complications
- Extracellular vesicles in disease
- Inflammatory Bowel Disease
- Ferroptosis and cancer prognosis
- Heme Oxygenase-1 and Carbon Monoxide
- Gut microbiota and health
- S100 Proteins and Annexins
- Pelvic floor disorders treatments
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Anorectal Disease Treatments and Outcomes
- Colorectal Cancer Screening and Detection
- Endoplasmic Reticulum Stress and Disease
- Food Allergy and Anaphylaxis Research
Shanghai Tenth People's Hospital
2014-2024
Tongji University
2014-2024
Tongji Hospital
2024
University of Pittsburgh
2016-2021
Huadong Hospital
2021
Fudan University
2021
Shandong Tumor Hospital
2021
Shandong First Medical University
2021
Anhui Medical University
2020
State Council of the People's Republic of China
2018
The inducible nitric oxide synthase (iNOS) is associated with more aggressive solid tumors, including hepatocellular carcinoma (HCC). Notch signaling in cancer stem cells promotes progression and requires cleavage by ADAM (a disintegrin metalloprotease) proteases. We hypothesized that iNOS/NO Notch1 activation through TACE/ADAM17 liver (LCSCs), leading to a phenotype. Expression of the cell markers CD24 CD133 tumors patients HCC was greater iNOS expression worse outcomes. CD24+CD133+ LCSCs,...
High mobility group box-1 (HMGB1), a ubiquitous nuclear protein, acts as late mediator of lethality when released extracellularly during sepsis. The major source circulating HMGB1 in sepsis is hepatocytes. However, the mechanism release hepatocytes not very clear. We have previously shown that bacterial endotoxin (LPS) sensing pathways, including TLR4 and caspase-11 regulate hepatocyte response to LPS. Here, we report novel function GSDMD LPS-induced active from endotoxemia was...
Abstract Disrupted gastrointestinal (GI) motility is highly prevalent in patients with inflammatory bowel disease (IBD), but its potential causative role remains unknown. Herein, the and mechanism of impaired GI colitis pathogenesis are investigated. Increased colonic mucosal inflammation found chronic constipation (CC). Mice dysmotility induced by genetic mutation or chemical insult exhibit increased susceptibility to colitis, dependent on gut microbiota. markedly decreases abundance...
Dysregulated host-microbial interactions play critical roles in initiation and perpetuation of gut inflammation Crohn's disease (CD). However, the spatial distribution interaction network across intestine its accessory tissues are still elusive. Here, we profile host proteins tissue microbes 540 samples from intestinal mucosa, submucosa-muscularis-serosa, mesenteric adipose tissues, mesentery, lymph nodes 30 CD patients spatially decipher interactions. We observe aberrant antimicrobial...
Abstract Re-expression of an embryonic morphogen, Nodal, has been seen in several types malignant tumours. By far, studies about Nodal’s role colorectal cancer (CRC) remain limited. Ferroptosis is essential for CRC progression, which caused by cellular redox imbalance and characterized lipid peroxidation. Herein, we observed that Nodal enhanced cell’s proliferative rate, motility, invasiveness, epithelial–mesenchymal transition (EMT) vivo vitro. Notably, overexpression induced...
The role and regulators of extracellular vesicle (EV) secretion in hepatic ischemia/reperfusion (IR) injury have not been defined. Rab27a is a guanosine triphosphatase known to control EV release. Interferon regulatory factor 1 (IRF‐1) transcription that plays an important liver IR regulates certain triphosphatases. However, the relationships among IRF‐1, Rab27a, are largely unknown. Here, we show induction IRF‐1 both vitro hypoxic hepatocytes vivo warm orthotopic transplantation livers. γ...
Tumour-derived exosomes (TEXs) have a potential for application in cancer vaccines. Whether TEXs after induction by interferon regulatory factor 1 (IRF-1) are capable of enhancing the antitumour response remains to be determined. Exosomes released tumour cells infected with IRF-1-expressing adenovirus (IRF-1-Exo) or treated interferon-γ (IFN-Exo) were isolated via ultracentrifugation. The IRF-1 target proteins IL-15Rα and MHC class I (MHC-I) analysed western blot. along CpG adjuvant injected...
Abstract Background Intestinal fibrosis is the final pathological outcome of chronic intestinal inflammation without specific therapeutic drugs, which leads to ileus and surgical intervention. characterized by excessive deposition extracellular matrix (ECM). The role mast cells (MCs), are members sentinel immune cell population, unknown in fibrosis. Methods In this study, we analyzed changes MCs, tryptase proteins, ECM components human fibrotic control patient intestines. We constructed...
piRNA-823 as a member of the piRNA family is reported to promote tumour cell proliferation in multiple myeloma and hepatocellular cancer. However, few studies on function colorectal cancer (CRC). Our present study data showed that plays an oncogene role CRC cells. Inhibition can significantly inhibit proliferation, invasion apoptosis resistance Mechanism have shown inhibits ubiquitination hypoxia-inducible factor-1 alpha (HIF-1α) by up-regulating expression Glucose-6-phosphate dehydrogenase...
Despite the role of donor-specific antibodies (DSAs) in recognizing major histocompatibility complex (MHC) antigens and mediating transplant rejection, how where recipient B cells lymphoid tissues encounter donor MHC remains unclear. Contrary to dogma, we demonstrated here that migration leukocytes out skin or heart allografts is not necessary for T cell allosensitization mice. We found mouse cardiac human grafts release cell-free via extracellular vesicles (EVs) are captured by subcapsular...
The detailed molecular mechanism between type 2 diabetes mellitus (T2DM) and colorectal cancer (CRC) is still uncertain. Bone morphogenetic protein 4 (BMP4) dysregulation implicated in T2DM CRC, respectively. This study aims to investigate whether BMP4 can mediate the interaction of CRC with T2DM.
Abstract Mast cells (MCs) are potent tissue-resident immune that distributed in the intraepithelial space of intestine and have been implicated regulating homeostasis coordinating epithelial responses inflamed mucosa inflammatory bowel disease (IBD). IL-33 functions as an endogenous danger signal or alarmin segments. MCs highly express receptor ST2. However, mechanisms underlying regulation MC-dependent IL-33/ST2 signaling at barrier surface remain largely unknown. We confirmed required for...
Purpose: The crosstalk between hepatocellular carcinoma (HCC) cells and hepatic stellate (HSCs) is one of the important mechanisms liver cancer metastasis. relationship metastasis glycolysis has been extensively studied recently. However, role von Willebrand factor (vWF) mediated mechanism in currently unknown. Methods: Western blot was used to verify expression vWF HCC cells. PAS staining, glycogen L-lactate content assays were reflect cellular levels. ability cell migration explored by...
Interferon regulatory factor-1 (IRF-1) is a tumor-suppressor gene induced by interferon-γ (IFNγ) and plays an important role in the cell death of hepatocellular carcinoma (HCC). HCC tumors evade part downregulating IRF-1 expression, yet molecular mechanisms accounting for suppression have not been characterized. Previous studies shown that microRNA-23a (miR-23a) can suppress apoptosis targeting IRF-1. Therefore, we hypothesized miR-23a promotes growth For vivo studies, 7 cases resected...
Immune cell infiltration is important for predicting the clinical outcomes of colorectal cancer. Integrin β7 (ITGB7), which expressed on surface leukocytes, plays an essential role in homing immune cells to gut-associated lymphoid tissue and facilitating retention lymphocytes gut epithelium; however, its cancer pathogenesis poorly explored. Here, we found that number β7+ decreased significantly tumor compared with adjacent normal tissue. expression tumor-derived tissue-derived CD8+ T cells....
Crohn's disease (CD) has an increasing incidence and prevalence worldwide. The etiology of CD remains unclear there is no gold standard for diagnosis. dysregulated immune response different infiltration status cells are critical pathogenesis; therefore, it important to provide overview immune-cell alterations in explore a novel method auxiliary Here we analyzed microarray datasets from Gene Expression Omnibus (GEO), extended version Cell-type Identification By Estimating Relative Subsets Of...
Ischemia and reperfusion (I/R) induces cytokines, up-regulates inducible nitric oxide synthase (iNOS), interferon regulatory factor-1(IRF1) p53 up-regulated modulator of apoptosis (PUMA), which contribute to cell death tissue injury. However, the mechanisms that I/R IRF1-PUMA through iNOS/NO is still unknown.Ischemia was induced by occluding structures in portal triad (hepatic artery, vein, bile duct) left median liver lobes for 60 min, initiated removal clamp. Induction iNOS, IRF1 PUMA...
Background: Few studies have assessed the short- and long-term outcomes of laparoscopically assisted natural orifice specimen extraction (NOSE) in patients with sigmoid colon rectal tumors. We investigated undergoing laparoscopic-assisted NOSE for tumors rectum. Methods: Ninety-eight were included. The tumor was classified according to its distance from anal verge: Group 1 (15–30 cm), 2 (5–15 3 (≤5 cm). In patients, a laparoscopic surgical collection bag used as special transrectal device....