MicroRNAs (miRNAs), endogenous small non-coding RNAs, are stably detected in human plasma. Early diagnosis of gastric cancer (GC) is very important to improve the therapy effect and prolong survival patients. We aimed identify whether four miRNAs (miR-223, miR-21, miR-218 miR-25) closely associated with tumorigenesis or metastasis GC can serve as novel potential biomarkers for detection.We initially measured plasma levels 10 patients healthy control subjects by quantitative reverse...

MicroRNA‐155 (miR‐155) has been implicated as a central regulator of the immune system. We have previously reported that miR‐155 negatively regulates Helicobacter pylori ( H. )‐induced inflammation, but molecular mechanism regulating inflammation is not fully clear. Here, we identified myeloid differentiation protein 88 (MyD88) target gene miR‐155, and found decreased MyD88 expression at mRNA message level, suggesting miR‐155‐mediated inhibition post‐transcriptional event. Furthermore,...

The differentiation and function of IL-17-producing Th17 cells are tightly regulated by specific transcription factors cytokines, which the key participants in pathogenesis multiple sclerosis (MS) experimental autoimmune encephalomyelitis (EAE). Although miRNAs have been shown to be involved development MS EAE, potential role context Th17-driven autoimmunity is just beginning clarified. miR-20b has reported as a downregulated miRNA blood patients. In this report, it was further studied...

Little is known about the potential role of microRNAs (miRNAs) in carcinogenesis gastric cancer induced by Helicobacter pylori ( H. ). Here, we showed that microRNA‐222 (miR‐222) was up‐regulated ‐infected mucosa and cancer. Ectopic expression miR‐222 promoted cell proliferation colony formation vitro. Mechanistically, identified RECK as a novel target miR‐222, also confirmed their relationship inverse correlation mRNA ex vivo. Furthermore, found RNA interference silencing can mimic...

Background Gastric cancer is one of the most common malignant diseases worldwide. Emerging evidence has shown that microRNAs (miRNAs) are associated with tumor development and progression. Our previous studies have revealed H. pylori infection was able to induce altered expression miR-30b in gastric epithelial cells. However, little known about potential role cancer. Methods We analyzed cell lines human tissues. examined effect mimics on apoptosis cells vitro by flow cytometry (FCM)...

Abstract Cervical cancer (CC) is a malignant solid tumor, which one of the main causes morbidity and mortality in women. Persistent High-risk human papillomavirus (hrHPV) infection closely related to cervical autophagy has been suggested inhibit viral infections. miRNAs have reported regulate many tumors with studies implicating miR-224-3p regulation autophagy. In this study, we performed miRNA microarray analysis on CC tissues found that large number differential expressions hrHPV-infected...

Detailed understanding of molecular mechanisms controlling adipogenesis is great importance to identify new targets for treating obesity. Emerging evidence suggests that long noncoding RNAs (lncRNAs) may play a pivotal role in adipogenesis. Here, we have identified novel lncRNA, Plnc1, which transcribed from position ~25,000 bp upstream the peroxisome proliferator-activated receptor γ2(PPAR-γ2) gene. Plnc1 abundantly expressed adipose tissue, and obese mice higher expression tissue than...

miR-196b-5p plays a role in various malignancies. We have recently reported its function regulating adipogenesis. However, it remains to be clarified whether and how affects bone cells homeostasis. In this study, vitro functional experiments showed an inhibitory effect of on osteoblast differentiation. Mechanistic explorations revealed that directly targeted semaphorin 3a (Sema3a) inhibited Wnt/β-catenin signaling. SEMA3A attenuated the impaired osteogenesis induced by miR-196b-5p....

ABSTRACT Proper differentiation of bone marrow stromal cells (BMSCs) into adipocytes is crucial for maintaining skeletal homeostasis. However, the underlying regulatory mechanisms remain incompletely understood, posing a challenge treatment age‐related osteopenia and osteoporosis. Here, through comprehensive gene expression analysis during BMSC adipocytes, we identified forkhead transcription factor Foxk2 as key regulator this process. was significantly higher in inguinal epididymal white...

Abstract Neural EGFL-like 2 (NELL2) is a secreted protein known for its regulatory functions in the nervous and reproductive systems, yet role bone biology remains unexplored. In this study, we observed that NELL2 was diminished of aged ovariectomized (OVX) mice, as well serum osteopenia osteoporosis patients. vitro loss-of-function gain-of-function studies revealed facilitated osteoblast differentiation impeded adipocyte from stromal progenitor cells. vivo further demonstrated deletion...

Nuclear factor I-C (NFIC) has recently been identified as an important player in osteogenesis and bone homeostasis vivo. However, the molecular mechanisms involved have yet to be defined. In current study, Nfic expression was altered primary marrow stromal cells established progenitor lines after adipogenic osteogenic treatment. Overexpression of ST2, mesenchymal C3H10T1/2, inhibited differentiation, whereas it promoted differentiation. Conversely, silencing endogenous cell enhanced blocked...

// Endong Zhu 1 , Yang Juanjuan Zhang Yongmei Li 1, 2 Chunjun Liming Chen Bei Sun Key Laboratory of Hormones and Development (Ministry Health), Tianjin Metabolic Diseases, Diseases Hospital Institute Endocrinology, Medical University, 300070 Tianjin, China Department Human Anatomy Histology, Correspondence to: Chen, email: xfx22081@vip.163.com Sun, sun_peipei220@hotmail.com Keywords: liraglutide, white fat browning, obesity, soluble guanylyl cyclase, uncoupling protein Received: September...

miR-20a-5p has recently been identified to induce adipogenesis of established adipogenic cell lines in our previous study. However, its role and molecular mechanisms the regulation adipocyte lineage commitment bone marrow-derived stromal cells (BMSCs) still need be explored. In this report, we demonstrated expression was promoted gradually during differentiation BMSCs. We also confirmed that a positive function BMSCs by gain-of-function study with overexpression lentivirus or synthetic...

Liraglutide, as a glucagon-like peptide‑1 analogue, is used to treat type 2 diabetes mellitus and obesity. Previous findings have demonstrated the effects of liraglutide on adipogenesis; however, underlying mechanism involved in this process remains be elucidated. In present study, certify effect adipogenesis explore possible process, preadipocyte 3T3‑L1 cells were cultured adipocyte‑inducing medium treated with liraglutide. Subsequently, expression levels master transcription factors...

Abstract Background N-myc downstream regulated gene 1 (NDRG1) plays a role in variety of biological processes including differentiation osteoclasts. However, it is not known if and how NDRG1 regulates osteogenic marrow stromal progenitor cells. Methods Gene expression profiling analysis was performed to study the level Ndrg1 during adipogenic differentiation. Gain-of-function and/or loss-of function experiments were carried out proliferation cells mechanism underlying investigated. Finally,...

Abstract Background Oncostatin M receptor (OSMR), as one of the receptors for oncostatin (OSM), has previously been shown to mediate stimulatory role OSM in osteoclastogenesis and bone resorption. However, it remains be clarified whether how OSMR affects differentiation osteoblasts. Methods The expression level during osteoblast adipocyte was examined. investigated using vitro gain-of-function loss-of-function experiments. mechanisms by which regulates cell were explored. Finally, vivo...

Abstract Adipogenesis is a finely controlled process and its dysfunction may contribute to metabolic disorders such as obesity. Metastasis suppressor 1 (MTSS1) player in tumorigenesis metastasis of various types cancers. To date, it not known whether how MTSS1 plays role adipocyte differentiation. In the current study, we found that was upregulated during adipogenic differentiation established mesenchymal cell lines primary cultured bone marrow stromal cells. Gain‐of‐function...

Abstract miR‐142a‐5p plays critical roles in multiple biological processes and diseases, such as inflammation tumorigenesis. However, it remains to be explored if how contributes osteoblast differentiation. In this study, our results showed that was highly expressed bone tissue of mice increased during osteogenesis preosteoblast MC3T3‐E1 cells. Supplementing activity using agomir promoted osteogenic differentiation stromal cell line ST2 preosteoblastic MC3T3‐E1. Conversely, antagomir, an...

It was previously reported that the loss of transcription factor nuclear I/X (NFIX) gene in mice impaired endochondral ossification and mineralization bone. However, cellular molecular basis for defect remains unexplored. In this study, we investigated if how NFIX regulates osteoblast differentiation. Nfix mRNA induced during osteogenic adipogenic differentiation progenitor cells. Loss-of-function gain-of-function studies revealed adipocyte formation from RNA-seq promoter analysis...

Naked cuticle homolog 2 (NKD2) has been recognized as an antagonist of Wnt/β-catenin signaling and a tumor suppressor. The role NKD2 in osteoblast osteoclast differentiation the mechanism are not fully understood. In this study, we identified up-regulation during adipocyte differentiation. Functional experiments revealed that stimulated suppressed formation. Furthermore, down-regulated expression receptor activator nuclear factor-κB ligand bone marrow mesenchymal stem cells inhibited...

Abstract Cortactin (CTTN), a cytoskeletal protein and substrate of Src kinase, is implicated in tumor aggressiveness. However, its role bone cell differentiation remains unknown. The current study revealed that CTTN was upregulated during osteoblast adipocyte differentiation. Functional experiments demonstrated promoted the vitro mesenchymal stem/progenitor cells into osteogenic adipogenic lineages. Mechanistically, able to stabilize level mechanistic target rapamycin kinase (mTOR), leading...