A5100411701- FOXO transcription factor regulation
- Nuclear Receptors and Signaling
- Circular RNAs in diseases
- RNA modifications and cancer
- Inflammasome and immune disorders
- Inflammatory Bowel Disease
- Genomics, phytochemicals, and oxidative stress
- Retinoids in leukemia and cellular processes
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Cancer-related gene regulation
- Bone Tumor Diagnosis and Treatments
- interferon and immune responses
- Evolution and Genetic Dynamics
- Renal and related cancers
- Bacterial Identification and Susceptibility Testing
- Heat shock proteins research
- Escherichia coli research studies
- Computational Drug Discovery Methods
- Blood disorders and treatments
- Bacterial biofilms and quorum sensing
- Chemokine receptors and signaling
- TGF-β signaling in diseases
- Probiotics and Fermented Foods
- Antibiotic Resistance in Bacteria
Nankai University
2024
Shandong Provincial QianFoShan Hospital
2024
Shandong First Medical University
2024
Chinese Academy of Sciences
2021-2023
Institute of Biophysics
2021-2023
University of Chinese Academy of Sciences
2023
Harbin Medical University
2022
Second Affiliated Hospital of Harbin Medical University
2022
Nanjing University of Posts and Telecommunications
2022
Qilu Hospital of Shandong University
2021
PRMT5 directly induces methylation of cGAS and inhibits cGAS-mediated antiviral immunity.
Abstract Cumulative exposure to solar ultraviolet (SUV) irradiation is regarded as the major etiologic factor in development of skin cancer. The activation MAPK cascades occurs rapidly and vital regulation SUV-induced cellular responses. T-LAK cell–originated protein kinase (TOPK), an upstream activator MAPKs, heavily involved inflammation, DNA damage, tumor development. However, chemopreventive therapeutic effects specific TOPK inhibitors cancer have not yet been elucidated. In current...
Abstract Background Field cancerization is the process in which a population of normal or pre-malignant cells affected by oncogenic alterations leading to progressive molecular changes that drive malignant transformation. Aberrant DNA methylation has been implicated early cancer development non-small cell lung (NSCLC); however, studies on its role field (FC) are limited. This study aims identify FC-specific patterns could distinguish between lesions and tumor tissues NSCLC. Methods We...
Abstract The identification of oncogenic biomolecules as drug targets is an unmet need for the development clinically effective novel anticancer therapies. In this study, we report first time that opsin 4/melanopsin (OPN4) plays a critical role in pathogenesis non–small cell lung cancer (NSCLC) and potential target. Our study has revealed OPN4 overexpressed human tissues cells, inversely correlated with patient survival probability. Knocking down expression suppressed cells growth induced...
Background: TGF-β1 promotes cell proliferation in only some tumors and exerts bidirectional regulatory effects on the of fibroblasts. This study intends to explore whether mechanism is related increased expression Ski. Methods: Cell fibrosarcoma line L929 was assessed with an ELISA BrdU kit. The mRNA protein levels corresponding factors were measured by RT-qPCR, immunohistochemistry or Western blotting vitro vivo. Additionally, c-Ski knocked down using RNAi. Ski human dermatofibrosarcoma...
Enterohemorrhagic Escherichia coli (EHEC) is an important foodborne pathogen that infects humans by colonizing the large intestine. The genome of EHEC O157:H7 contains 177 unique O islands (OIs). Certain OIs significantly contribute to heightened virulence and pathogenicity exhibited O157:H7. However, function most OI genes remains unknown. We demonstrated here adherence colonization mouse intestine are both dependent on OI-97. Z3495, which annotated as a LysR-type transcriptional regulator...
The concept of synthetic lethality has great potential for anticancer therapy as a new strategy to specifically kill cancer cells while sparing normal cells. To further understand the molecular interactions and gene characteristics involved in lethality, we performed comprehensive analysis predicted cancer‐specific genetic interactions. Many genes were identified cancer‐associated that contributed multiple biological processes pathways, features not random, indicating their roles human...
<p>Supplementary materials and methods</p>
<div>Abstract<p>The key functional molecules involved in inflammatory bowel disease (IBD) and IBD-induced colorectal tumorigenesis remain unclear. In this study, we found that the apoptosis repressor with caspase recruitment domain (ARC) protein plays critical roles IBD. ARC-deficient mice exhibited substantially higher susceptibility to dextran sulfate sodium (DSS)-induced IBD compared wild-type mice. The burden induced conditions was inversely correlated CCL5 CXCL5 levels...
<div>Abstract<p>The key functional molecules involved in inflammatory bowel disease (IBD) and IBD-induced colorectal tumorigenesis remain unclear. In this study, we found that the apoptosis repressor with caspase recruitment domain (ARC) protein plays critical roles IBD. ARC-deficient mice exhibited substantially higher susceptibility to dextran sulfate sodium (DSS)-induced IBD compared wild-type mice. The burden induced conditions was inversely correlated CCL5 CXCL5 levels...
<div>Abstract<p><b>Purpose:</b> Investigate the role of regulator chromosome condensation 2 (<i>RCC2</i>) on lung adenocarcinoma (LUAD) metastasis.</p><p><b>Experimental Design:</b> Clinical specimens were used to assess impact RCC2 LUAD metastasis. Mouse models, cytobiology, and molecular biology assays performed elucidate function underlying mechanisms in LUAD.</p><p><b>Results:</b> expression was...
<p>Supplemental Figure Legend</p>
<p>ADA-07 significantly suppresses SUV-induced skin carcinogenesis in SKH-1 hairless mice (early-stage prevention). A, average body weight of mice. Mice were weighed once a week until the end study at 28. B, external appearance tumors. treated as described Materials and Methods. The control groups (n = 9) received no vehicle/no SUV 3), or 1 mg ADA-07/no respectively. group only 12) vehicle/SUV-treated with oil-in-water emulsion cream before exposure. 0.1 mg/SUV 12 each) treatment...
<p>ADA-07 significantly suppresses SUV-induced skin carcinogenesis in SKH-1 hairless mice (late-stage prevention). A, average body weight of mice. Mice were weighed once a week until 28. B, external appearance tumors. treated as described Materials and Methods. The the control groups (n = 9) received no vehicle/no SUV 3), or 1 mg ADA-07/no respectively. rest irradiation for 15 weeks 3 times week. vehicle/SUV-treated group 12) with oil-in-water emulsion cream end experiment (28 weeks)....
<p>ADA-07 synthesis, MS analysis and cytotoxicity assays.</p>
<p>The expression level of TOPK in normal skin cells (JB6 P+, NHDF) and cancer (A431 SCC12). was assessed by Western blot using a specific antibody β-actin used as loading control.</p>
<div>Abstract<p>Cumulative exposure to solar ultraviolet (SUV) irradiation is regarded as the major etiologic factor in development of skin cancer. The activation MAPK cascades occurs rapidly and vital regulation SUV-induced cellular responses. T-LAK cell–originated protein kinase (TOPK), an upstream activator MAPKs, heavily involved inflammation, DNA damage, tumor development. However, chemopreventive therapeutic effects specific TOPK inhibitors cancer have not yet been...
<p>Supplemental Figure Legend</p>
<p>ADA-07 significantly suppresses SUV-induced skin carcinogenesis in SKH-1 hairless mice (late-stage prevention). A, average body weight of mice. Mice were weighed once a week until 28. B, external appearance tumors. treated as described Materials and Methods. The the control groups (n = 9) received no vehicle/no SUV 3), or 1 mg ADA-07/no respectively. rest irradiation for 15 weeks 3 times week. vehicle/SUV-treated group 12) with oil-in-water emulsion cream end experiment (28 weeks)....
<p>Measurement of ADA-07 absorbance by spectrophotometer. Absorbance wavelengths were scanned using a Beckman DU®800 spectrophotometer.</p>
<p>ADA-07 synthesis, MS analysis and cytotoxicity assays.</p>
<p>The expression level of TOPK in normal skin cells (JB6 P+, NHDF) and cancer (A431 SCC12). was assessed by Western blot using a specific antibody β-actin used as loading control.</p>
Gene silencing by P-element-induced wimpy testis-interacting RNAs is a mechanism to maintain genome integrity in germ cells. Here, we present protocol for knockin or knockout editing of male germline mediated CRISPR-Cas9 technology Caenorhabditis elegans. We describe steps constructing edited plasmids, microinjecting worms with these and screening worms. then detail procedures dissecting released sperm their observation fluorescence microscopy. Engineered provide model studying...