A5059429305- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Bone and Joint Diseases
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Liver physiology and pathology
- Transplantation: Methods and Outcomes
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Diabetic Foot Ulcer Assessment and Management
- Neonatal Respiratory Health Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Periodontal Regeneration and Treatments
- Immunotherapy and Immune Responses
- Bone Metabolism and Diseases
- CAR-T cell therapy research
- Advanced Glycation End Products research
- Liver Disease Diagnosis and Treatment
Fujian Medical University
2024
Beijing VDJBio (China)
2022
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2000-2021
Beijing Institute of Education
2020-2021
Guangdong Province Women and Children Hospital
2013-2020
Beijing Institute of Neurosurgery
2020
Cell Technology (China)
2020
Academy of Military Medical Sciences
2009-2018
Guangzhou Medical University
2015-2018
Beijing Radiation Center
2008-2015
Although mesenchymal stem cells (MSCs) have been increasingly trialed to treat a variety of diseases, the underlying mechanisms remain still elusive. In this study, human umbilical cord (UC)-derived MSCs were stimulated by hypoxia, and membrane microvesicles (MVs) in supernatants collected ultracentrifugation, observed under an electron microscope, origin was identified with flow cytometric technique. The results showed that upon hypoxic stimulus, released large quantity MVs ∼100 nm...
Bone marrow contains a population of rare progenitor cells capable differentiating into osteoblasts, chondrocytes, adipocytes, myoblasts, and hematopoiesis-supporting stromal cells. These cells, referred to as mesenchymal (MPCs), can be purified culture-expanded from animals humans. Using bone-marrow-conditioned medium combined with basic fibroblast growth factor, we cultured relatively homogeneous MPCs murine bone marrow, which uniformly expressed stem cell antigen-1, CD29, CD44, c-kit,...
Mesenchymal stem cells (MSCs) have been approved as a cellular drug for the treatment of variety immune-related diseases by government many countries'. Previous investigations, including ours, shown that exosomes secreted MSCs (MSC-ex) are one main factors responsible therapeutic effect MSCs. However, immune modulation activities and contents MSC-ex derived from under different incubation conditions differ dramatically. Therefore, optimal way to ensure effectiveness is identifying preparing...
Background Previous data have proven that microvesicles derived from hypoxia-induced mesenchymal stem cells (MSC-MVs) can be internalized into endothelial cells, enhancing their proliferation and vessel structure formation promoting in vivo angiogenesis. However, there is a paucity of information about how the MSC-MVs are up-taken by cells. Methods MVs were prepared supernatants human bone marrow MSCs had been exposed to hypoxic and/or serum-deprivation condition. The incorporation umbilical...
Abstract Due to the potent immunoregulatory capacity, mesenchymal stem cells (MSCs) have been used in clinical trials treat acute graft-versus-host disease (aGvHD), although detailed vivo mechanisms remain elusive. In a murine lethal aGvHD model, MSCs delayed development of disease. Interestingly, we found that MSC infusion increased number T lymphocytes secondary lymphoid organs (SLOs). Since expression CD62L and CCR7 is prerequisite for lymphocyte migration into SLOs, vitro experiments...
Introduction & Objective Microvesicles (MVs) derived from mesenchymal stem cells (MSCs) have been shown to promote angiogenesis. This study was aimed shed a light on the mechanisms by analyzing angiogenesis-promoting compositions of MSC-MVs. Also we try figure out impact hypoxia Methods MVs were isolated culture supernatants MSCs under hypoxia/normoxia and serum-deprivation condition. The morphological features revealed an electron microscope origin identified bead-bound assay. An antibody...
Abstract In contrast to the considerable amount of data that documents biological properties mesenchymal progenitor cells from human and other species, there is still paucity information about mouse counterparts, as their purification culture expansion procedures remain rudimentary. present study, murine cell (muMPC) was developed by explant collagenase-digested bone fragments after removal released cells. During cultivation, fibroblastoid sprouted migrated fragments, followed adherent...
Extracellular high mobility group box 1 (HMGB1) is a novel cytokine that takes part in the processes of in: ammation, tissue damage and regeneration. Mesenchymal stem cells (MSCs) are adult characterized by their inherently suppressive activities on inflammative allo-immune reactions. In present study, we have addressed whether HMGB1 could affect biological properties human bone marrow MSCs. Transwell experiments showed induced MSC migration this effect not be hampered blocking antibody...
We report here the preliminary results of allogeneic hematopoietic stem cell transplantation with mesenchymal cells (MSCs) for 6 cases severe aplastic anemia. The patients ranged in age from 3 to 16 years, and median time diagnosis was 32 months (range: 3-156 months). conditioning regimens consisted fludarabine, cyclophosphamide, antithymocyte globulin or without busulfan. Graft-versus-host disease (GvHD) prevented by administration cyclosporine A, methotrexate, mycophenolate mofetil,...
Pulmonary silicosis is an irreversible and untreatable disease that characterized by interstitial lesions perpetual fibrosis in the lungs.This study was performed to determine whether mesenchymal stem cells (MSCs) hepatocyte growth factor (HGF) could exhibit therapeutic effects on human silicosis.This nonrandomized uncontrolled trial comprised four patients with pulmonary who had developed lung received autologous bone marrow MSCs previously transfected a vector containing HGF cDNA...
Abstract Background Though accumulated evidence has demonstrated visceral organ involvement in acute graft-versus-host disease (aGVHD), how aGVHD influences the bone marrow (BM) niche and reconstitution of hematopoiesis post-hematopoietic stem cell transplantation remains largely unknown. Methods In current study, morphology, immunophenotype, multi-differentiation capacity, self-renewal promotion MSCs from non-aGVHD patients were investigated. Additionally, stemness hematopoiesis-promoting...
Mesenchymal stem cells (MSCs) are characterized by their hematopoiesis-supporting and immunosuppressive capacity, while osteoclasts main cell components in the endosteal hematopoietic niche pivotal players osteoimmunology. To clarify association of these 2 kinds cells, mouse CD11b(+) monocytes were cultured onto MSC layers presence or absence macrophage colony-stimulating factor (M-CSF) receptor activator NF-kappaB ligand (RANKL). The results showed that MSCs independently supported...
Haploidentical Hematopoietic stem cell transplantation (Haplo-HSCT) has provided an alternative option since virtually all patients have immediately available donor. Here, we report the results of Haplo-HSCT with granulocyte-colony-stimulating factor (G-CSF) mobilized bone marrow grafts plus peripheral blood cells as without T-cell depletion. Twenty-nine mean age 27.27 years (ranging from 15 to 51 years) were enrolled in this study, and 10 cases high risk status. The received myeloablative...
In this report, the authors describe a protocol for haploidentical bone marrow transplantation in children who received G-CSF-mobilized grafts without T-cell depletion from HLA-mismatched parents. Forty-two of 45 patients achieved complete donor hematopoietic engraftment; medium time neutrophil and platelet recovery was 17 19 days, respectively. Three died early transplantation-associated complications; other causes death included relapse (11 cases), fungal pneumonia (5), acute...
miR‐663 is a tumour suppressor that potentially regulated by modification of C p G islands. Whether aberrant methylation one the reasons for down‐regulation in some malignant cells and whether targets oncogenes warrants further research. In present study, we report islands upstream region pre‐miR‐663 are aberrantly methylated k‐562 cell line white blood chronic myelogenous leukaemia patients, also H‐ras genes targeted miR‐663. Over‐expression may suppress proliferation part enhancing apoptosis.