A5000035593- Adipose Tissue and Metabolism
- Inflammatory Bowel Disease
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- IL-33, ST2, and ILC Pathways
- Epigenetics and DNA Methylation
- Diabetes and associated disorders
- Diet and metabolism studies
- Metabolism, Diabetes, and Cancer
- Mesenchymal stem cell research
- Immune cells in cancer
- Biomarkers in Disease Mechanisms
- Genetics and Neurodevelopmental Disorders
- Gestational Diabetes Research and Management
- Lipid metabolism and disorders
- Cancer Cells and Metastasis
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Autophagy in Disease and Therapy
- Diabetes Treatment and Management
- Birth, Development, and Health
- Diet, Metabolism, and Disease
- Cardiovascular Disease and Adiposity
- Autoimmune and Inflammatory Disorders
- Reproductive System and Pregnancy
- Axon Guidance and Neuronal Signaling
Institut d'Investigació Sanitària Pere Virgili
2015-2024
Universitat Rovira i Virgili
2015-2024
Inserm
2023
Institut de génétique et de biologie moléculaire et cellulaire
2023
Centre National de la Recherche Scientifique
2023
Université de Strasbourg
2023
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2011-2022
Instituto de Salud Carlos III
2015-2022
Hospital Universitari Joan XXIII de Tarragona
2019
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2011-2016
Adipose tissue-derived stem cells (ASCs) are proposed as an alternative cell source to bone marrow-derived for immune therapy. However, microenvironmental factors may impact the functionality of this population in human adipose tissue (AT). We hypothesized that fat depot addition donor phenotype controls immunomodulatory capacity ASCs. Focusing on obesity and type 2 diabetes (T2D) metabolic disorders might affect response ASCs, we compared inflammatory ASCs from subcutaneous visceral AT...
Adipose tissue is a major source of mesenchymal stem cells (MSCs), which possess variety properties that make them ideal candidates for regenerative and immunomodulatory therapies. Here, we compared the immunophenotypic profile human adipose-derived (hASCs) from lean obese individuals, explored its relationship with apparent altered plasticity hASCs. We also hypothesized persistent hypoxia treatment cultured hASCs may be necessary but not sufficient to drive significant changes in mature...
A functional population of adipocyte precursors, termed adipose-derived stromal/stem cells (ASCs), is crucial for proper adipose tissue (AT) expansion, lipid handling, and prevention lipotoxicity in response to chronic positive energy balance. We previously showed that obese human subjects contain a dysfunctional pool ASCs. Elucidation the mechanisms underlying abnormal ASC function might lead therapeutic interventions by improving adipogenic capacity Using epigenome-wide association...
Readily accessible human pancreatic beta cells that are functionally close to primary adult a crucial model better understand cell physiology and develop new treatments for diabetes. We here report the characterization of EndoC-βH5 cells, latest in EndoC-βH family.EndoC-βH5 were generated by integrative gene transfer immortalizing transgenes hTERT SV40 large T along with Herpes Simplex Virus-1 thymidine kinase into fetal pancreas. Immortalizing removed after amplification using CRE...
Glycogen metabolism has emerged as a mediator in the control of energy homeostasis and studies murine models reveal that adipose tissue might contain glycogen stores. Here we investigated physio(patho)logical role human context obesity insulin resistance. We studied glucose metabolic flux hypoxic adipoctyes by nuclear magnetic resonance mass spectrometry-based approaches. synthesis content response to hypoxia was analyzed adipocytes macrophages. To explore effects enforced deposition...
Crohn's disease (CD) is characterized by the expansion of mesenteric fat, also known as "creeping fat." We explored plasticity and immune properties adipose-derived stem cells (ASCs) in context CD potential key players development creeping fat. Mesenteric CD-derived ASCs presented a more proliferative, inflammatory, invasive, phagocytic phenotype than equivalent from healthy donors, irrespective clinical stage. Remarkably, subcutaneous depot patients with showed an activated response that...
Pancreatic β-cell dysfunction is a key feature of type 2 diabetes, and novel regulators insulin secretion are desirable. Here we report that the succinate receptor (SUCNR1) expressed in β-cells up-regulated hyperglycemic states mice humans. We found acts as hormone-like metabolite stimulates via SUCNR1-Gq-PKC-dependent mechanism human β-cells. Mice with β-cell-specific Sucnr1 deficiency exhibit impaired glucose tolerance on high-fat diet, indicating SUCNR1 essential for preserving...
Abstract We aimed to explore the relationship between GLP-1 receptor ( GLP-1R ) expression in adipose tissue (AT) and incretin secretion, glucose homeostasis weight loss, patients with morbid obesity type 2 diabetes undergoing bariatric surgery. RNA was extracted from subcutaneous (SAT) visceral (VAT) AT biopsies 40 randomized metabolic gastric bypass, sleeve gastrectomy or greater curvature plication. Biochemical parameters, fasting plasma insulin, glucagon area under curve (AUC) of...
Background & AimsEnteroendocrine cells (EECs) and their hormones are essential regulators of whole-body energy homeostasis. EECs sense luminal nutrients microbial metabolites subsequently secrete various acting locally or at a distance. Impaired development during embryogenesis is life-threatening in newborn mice humans due to compromised nutrient absorption. However, the physiological importance EEC system adult has yet be directedly studied. Herein, we aimed determine long-term...
Abstract Background and Aims Crohn’s disease [CD] is characterised by the expansion of mesenteric adipose tissue [MAT], named creeping fat [CF], which seems to be directly related activity. Adipose-stem cells [ASCs] isolated from CF patients with CD are extremely pro-inflammatory, persists during remission. We hypothesised that dysfunctional ASCs in accumulate epigenetic modifications triggered inflammatory environment, could serve as molecular markers. Methods Genome-wide DNA methylome...
Abstract Background Crohn’s disease (CD) is characterized by persistent inflammation and ulceration of the small or large bowel, expansion mesenteric adipose tissue, termed creeping fat (CF). We previously demonstrated that human adipose-derived stem cells (hASCs) from CF patients with CD exhibit dysfunctional phenotypes, including a pro-inflammatory profile, high phagocytic capacity, weak immunosuppressive properties. Importantly, these phenotypes persist in remission are found all depots...
Abstract Purpose Recent studies point to adipose-derived stem cells (ASCs) as a link between obesity and cancer. We aimed determine whether survivin, which is highly secreted by ASCs from subjects with obesity, might drive pro-tumoral phenotype in macrophages. Methods The effect of ASC conditioned medium on the macrophage was assessed expression studies. Survivin intracellular localization internalization were examined subcellular fractionation immunofluorescence, respectively. Loss-...
Abstract Adipose tissue (AT) has a central role in obesity-related metabolic imbalance through the dysregulated production of cytokines and adipokines. In addition to its known risk for cardiovascular disease diabetes, obesity is also major cancer. We investigated impact expression survivin, an antiapoptotic protein upregulated by adipokines diagnostic biomarker tumor onset recurrence. cross-sectional study 111 subjects classified body mass index, circulating levels survivin gene...
DNA methylation in the diagnosis of gestational diabetes.To assess value diabetes (GDM) and prediction maternal postpartum glucose disturbances.Two-stage observational study performed between July 2006 December 2010, at University Hospital. Forty-eight randomly selected pregnant women formed discovery cohort (24 with GDM 24 controls) 252 (94 158 replication cohort. were re-evaluated 4 years postpartum. The main outcome measures GDM, type 2 or prediabetes postpartum.We identified 3 CpG sites...
Evidence from mouse models suggests that zinc-α2-glycoprotein (ZAG) is a novel anti-obesity adipokine. In humans, however, data are controversial and its physiological role in adipose tissue (AT) remains unknown. Here we explored the molecular mechanisms by which ZAG regulates carbohydrate metabolism human adipocytes.ZAG action on glucose uptake insulin was analyzed. β1 β2-adrenoreceptor (AR) antagonists siRNA targeting PP2A phosphatase were used to examine modulates sensitivity. Plasma...
Abstract Fetal programming has been proposed as a key mechanism underlying the association between intrauterine exposure to maternal diabetes and negative health outcomes in offspring. To determine whether gestational mellitus (GDM) might leave an imprint fetal precursors of amniotic membrane it be related adverse offspring, prospective case-control study was conducted, which mesenchymal stem cells (AMSCs) resident macrophages were isolated from pregnant patients, with either GDM or normal...
This epigenome-wide association study in stromal/stem cells (ASCs), derived from subcutaneous adipose tissue samples of lean and obese subjects, revealed a specific DNA methylation signature adipocyte precursors associated with obesity, which has significant impact on the metabolic phenotype mitochondrial function mature adipocytes.
Atonal Homolog 8 (Atoh8) is a basic helix-loop-helix (bHLH) transcription factor that highly conserved across species and expressed in multiple tissues during embryogenesis. In the developing pancreas, Atoh8 endocrine progenitors but declines hormone-positive cells, suggesting role early stages of differentiation program. We previously generated whole-body knockout lethality null embryos precluded assessment functions organ development. Here we report generation conditional mouse strain by...
Adipose-derived mesenchymal stem cells (ASCs) are a promising option for the treatment of obesity and its metabolic co-morbidities. Despite recent identification brown adipose tissue (BAT) as potential target in management obesity, use ASCs isolated from BAT therapy patients with has not yet been explored. Metabolic activation shown to have only thermogenic effects, but it also triggers secretion factors that confer protection against obesity. Herein, we characterized visceral surrounding...