A5007378385- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
- Cancer, Stress, Anesthesia, and Immune Response
- Adipose Tissue and Metabolism
- Inflammatory Bowel Disease
- Epigenetics and DNA Methylation
- Lipid metabolism and disorders
- Gut microbiota and health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Immune cells in cancer
- Fibroblast Growth Factor Research
- IL-33, ST2, and ILC Pathways
- Metabolism, Diabetes, and Cancer
- Mesenchymal stem cell research
- Dietary Effects on Health
- Circadian rhythm and melatonin
- Atherosclerosis and Cardiovascular Diseases
- Cancer, Hypoxia, and Metabolism
- Apelin-related biomedical research
- Endoplasmic Reticulum Stress and Disease
- Autoimmune and Inflammatory Disorders
- Regulation of Appetite and Obesity
- Diet, Metabolism, and Disease
- Pancreatic function and diabetes
- Microscopic Colitis
Institut d'Investigació Sanitària Pere Virgili
2015-2024
Universitat Rovira i Virgili
2015-2024
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2015-2023
Instituto de Salud Carlos III
2015-2023
Hospital Universitari Joan XXIII de Tarragona
2023
Abstract Background Succinate is produced by both human cells and gut bacteria couples metabolism to inflammation as an extracellular signaling transducer. Circulating succinate elevated in patients with obesity type 2 diabetes linked numerous complications, yet no studies have specifically addressed the contribution of microbiota systemic or explored consequences reducing intestinal levels this setting. Results Using germ-free microbiota-depleted mouse models, we show that a significant...
Adipose tissue-derived stem cells (ASCs) are proposed as an alternative cell source to bone marrow-derived for immune therapy. However, microenvironmental factors may impact the functionality of this population in human adipose tissue (AT). We hypothesized that fat depot addition donor phenotype controls immunomodulatory capacity ASCs. Focusing on obesity and type 2 diabetes (T2D) metabolic disorders might affect response ASCs, we compared inflammatory ASCs from subcutaneous visceral AT...
A functional population of adipocyte precursors, termed adipose-derived stromal/stem cells (ASCs), is crucial for proper adipose tissue (AT) expansion, lipid handling, and prevention lipotoxicity in response to chronic positive energy balance. We previously showed that obese human subjects contain a dysfunctional pool ASCs. Elucidation the mechanisms underlying abnormal ASC function might lead therapeutic interventions by improving adipogenic capacity Using epigenome-wide association...
Succinate is produced by both host and microbiota, with a key role in the interplay of immunity metabolism an emerging as biomarker for inflammatory metabolic disorders middle-aged adults. The relationship between plasma succinate levels cardiovascular disease (CVD) risk young adults unknown.Cross-sectional study 100 (65% women) individuals aged 18-25 years from ACTIvating Brown Adipose Tissue through Exercise (ACTIBATE) cohort. CVD factors, body composition, dietary intake, basal rate,...
Succinate and succinate receptor 1 (SUCNR1) are linked to fibrotic remodeling in models of non-alcoholic fatty liver disease (NAFLD), but whether they have roles beyond the activation hepatic stellate cells remains unexplored. We investigated succinate/SUCNR1 axis context NAFLD specifically hepatocytes.We studied phenotype wild-type Sucnr1-/- mice fed a choline-deficient high-fat diet induce steatohepatitis (NASH), explored function SUCNR1 murine primary hepatocytes human HepG2 treated with...
Glycogen metabolism has emerged as a mediator in the control of energy homeostasis and studies murine models reveal that adipose tissue might contain glycogen stores. Here we investigated physio(patho)logical role human context obesity insulin resistance. We studied glucose metabolic flux hypoxic adipoctyes by nuclear magnetic resonance mass spectrometry-based approaches. synthesis content response to hypoxia was analyzed adipocytes macrophages. To explore effects enforced deposition...
Pancreatic β-cell dysfunction is a key feature of type 2 diabetes, and novel regulators insulin secretion are desirable. Here we report that the succinate receptor (SUCNR1) expressed in β-cells up-regulated hyperglycemic states mice humans. We found acts as hormone-like metabolite stimulates via SUCNR1-Gq-PKC-dependent mechanism human β-cells. Mice with β-cell-specific Sucnr1 deficiency exhibit impaired glucose tolerance on high-fat diet, indicating SUCNR1 essential for preserving...
Abstract Background Crohn’s disease (CD) is characterized by persistent inflammation and ulceration of the small or large bowel, expansion mesenteric adipose tissue, termed creeping fat (CF). We previously demonstrated that human adipose-derived stem cells (hASCs) from CF patients with CD exhibit dysfunctional phenotypes, including a pro-inflammatory profile, high phagocytic capacity, weak immunosuppressive properties. Importantly, these phenotypes persist in remission are found all depots...
Crohn's disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, barrier inflammation, and hyperplasia of creeping fat (CF) mesenteric adipose tissue (AT), which seems be directly related activity. Gut microbiota dysbiosis might a determining factor in CD etiology, manifesting as low microbial diversity high abundance potentially pathogenic bacteria. We tested hypothesis that CF reservoir bacteria through 16S-rRNA sequencing several AT depots...
Abstract Purpose Recent studies point to adipose-derived stem cells (ASCs) as a link between obesity and cancer. We aimed determine whether survivin, which is highly secreted by ASCs from subjects with obesity, might drive pro-tumoral phenotype in macrophages. Methods The effect of ASC conditioned medium on the macrophage was assessed expression studies. Survivin intracellular localization internalization were examined subcellular fractionation immunofluorescence, respectively. Loss-...
Abstract Adipose tissue (AT) has a central role in obesity-related metabolic imbalance through the dysregulated production of cytokines and adipokines. In addition to its known risk for cardiovascular disease diabetes, obesity is also major cancer. We investigated impact expression survivin, an antiapoptotic protein upregulated by adipokines diagnostic biomarker tumor onset recurrence. cross-sectional study 111 subjects classified body mass index, circulating levels survivin gene...
Evidence from mouse models suggests that zinc-α2-glycoprotein (ZAG) is a novel anti-obesity adipokine. In humans, however, data are controversial and its physiological role in adipose tissue (AT) remains unknown. Here we explored the molecular mechanisms by which ZAG regulates carbohydrate metabolism human adipocytes.ZAG action on glucose uptake insulin was analyzed. β1 β2-adrenoreceptor (AR) antagonists siRNA targeting PP2A phosphatase were used to examine modulates sensitivity. Plasma...
This epigenome-wide association study in stromal/stem cells (ASCs), derived from subcutaneous adipose tissue samples of lean and obese subjects, revealed a specific DNA methylation signature adipocyte precursors associated with obesity, which has significant impact on the metabolic phenotype mitochondrial function mature adipocytes.
Adipose-derived mesenchymal stem cells (ASCs) are a promising option for the treatment of obesity and its metabolic co-morbidities. Despite recent identification brown adipose tissue (BAT) as potential target in management obesity, use ASCs isolated from BAT therapy patients with has not yet been explored. Metabolic activation shown to have only thermogenic effects, but it also triggers secretion factors that confer protection against obesity. Herein, we characterized visceral surrounding...
Glucagon-like peptide-2 (GLP-2) is a gastrointestinal hormone released in response to nutritional intake that exerts wide range of effects by activating GLP-2 receptors. In addition its intestinotrophic effects, also positively influences glucose metabolism under conditions obesity, but the mechanisms behind this remain unclear. Here, we have investigated molecular role GLP-2/GLP-2 receptor axis energetic metabolism, focusing on potential modulatory adipose tissue.Physiological measurements...
Abstract Dysfunctional adipocyte precursors have emerged as key determinants for obesity‐ and aging‐related inflammation, but the mechanistic basis remains poorly understood. Here, we explored dysfunctional adipose tissue of elderly obese individuals focusing on metabolic inflammatory state human adipose‐derived mesenchymal stromal cells (hASCs), sirtuins, which link metabolism inflammation. Both obesity aging impaired differentiation potential hASCs had a different impact their...
White adipose tissue (WAT) explants culture allows the study of this ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism hormonal regulation. Here, we present a protocol for obtaining, isolating, processing adipocytes, alongside cultivation WAT from humans mice. We describe steps retrieval, culturing explants, digestion, separation. then detail procedures isolated adipocytes. For complete details on use...
Abstract Background Crohn’s disease (CD) is characterised by expansion of mesenteric adipose tissue, called creeping fat, which seems to be directly related activity. Adipose stem cells (ASCs) isolated from the fat CD patients exhibit dysfunction, featuring impaired adipogenesis and an intensely pro-inflammatory phenotype. This study aims explore transcriptome ASCs active inactive subjects in comparison with healthy subjects, seeking key markers this dysregulation. Methods Patients were...