A5100459216- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Nerve injury and regeneration
- RNA Research and Splicing
- Spinal Cord Injury Research
- Autophagy in Disease and Therapy
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- RNA modifications and cancer
- Lipid metabolism and disorders
- Vibrio bacteria research studies
- Adipose Tissue and Metabolism
- Plant Molecular Biology Research
- Endoplasmic Reticulum Stress and Disease
- Bacterial Identification and Susceptibility Testing
- Ferroptosis and cancer prognosis
- Fungal Biology and Applications
- Digestive system and related health
- Genetics, Aging, and Longevity in Model Organisms
- Caveolin-1 and cellular processes
First Affiliated Hospital of Harbin Medical University
2024
Guangzhou Medical University
2024
Tianjin Medical University
2012-2024
Anhui Academy of Agricultural Sciences
2024
Harbin Medical University
2024
Shandong University
2024
Hefei University of Technology
2023
Zunyi Medical University
2023
Beijing Haidian Hospital
2022
Southern Medical University
2019-2021
Abstract Ferroptosis is an iron-dependent cell death mechanism involving the accumulation of lipid peroxides. As a critical regulator, glutathione peroxidase 4 (GPX4) has been demonstrated to be downregulated in epilepsy. However, ferroptosis epilepsy remains unclear. In this study, bioinformatics analysis, analysis patient blood samples and mouse experiments revealed strong associations among epilepsy, ferroptosis, microRNA-211-5p purinergic receptor P2X 7 (P2RX7). P2RX7 nonselective...
Methionine (Met) plays a critical regulatory role in milk production, however, the molecular mechanism of action Met is largely unknown. This study therefore aimed to investigate influence on synthesis and proliferation bovine mammary epithelial cells (BMECs) explore underlying mechanism. BMECs cultured fetal serum (FBS) free Dulbecco's modified eagle's medium (DMEM)/F-12 were treated with (0, 0.3, 0.6, 0.9, 1.2 mM). Results showed that (0.6 mM) significantly increased protein fat cell...
The microRNA (miRNA) let-7 is an important miRNA identified in Caenorhabditis elegans and has been shown to be involved the control of innate immunity. underlying molecular mechanisms for regulation immunity remain largely unclear. In this study, we investigated basis intestinal Infection with Pseudomonas aeruginosa PA14 decreased let-7::GFP expression. Intestine- or neuron-specific activity was required its function During immune response P. infection, SDZ-24 as a direct target let-7. found...
Aberrant cancer metabolism contributes to cell proliferation and tumor progression. However, the contribution of enhanced glycolysis, observed during metabolism, pathogenesis progression nasopharyngeal carcinoma (NPC) remains unclear. CYLD, an NF-κB inhibitor, is frequently deficient in NPC. Here, we investigated role CYLD metabolic reprogramming NPC found that restoration expression suppressed glycolysis cells. Mechanistic dissection showed stabilized p53 facilitated its nuclear...
Abstract Background This study aimed to explore the association of long non‐coding RNA nuclear‐enriched abundant transcript 1 ( lncRNA NEAT1 ) with exacerbation risk, lung function, and inflammatory cytokines in asthma. Methods A total 170 patients asthma exacerbation, remission, healthy controls (HCs) were enrolled, their plasma samples collected. The expressions microRNA‐124 miRNA‐124 detected by real‐time quantitative polymerase chain reaction; measured Enzyme‐linked immunosorbent assay...
In the present study, we investigated intervention effects of a purified <i>Polygonatum cyrtonema</i> polysaccharide (PCP) on high-fat diet (HFD)-induced atherosclerosis in male and female LDLr<sup>−/−</sup> mice. Results showed that HFD caused severe dyslipidemia, atherosclerotic lesions, oxidative damages inflammation mice, these seemed to be more pronounced males than females. However, above variations could dose-dependently reversed by PCP treatment, were greater those NF-κB, MAPKs Akt...
The LJP61A suppressed macrophage foam cell formation and atherosclerotic progression by modulating Ca 2+ -calpain mediated autophagy inhibition.
Valproic acid (VPA), an anticonvulsant and mood-stabilizing drug, can induce neuronal differentiation, promote neurite extension exert a neuroprotective effect in central nervous system (CNS) injuries; however, comparatively little is known regarding its action on mouse embryonic neural stem cells (NSCs) the underlying molecular mechanism. Recent studies suggested that c-Jun N-terminal kinase (JNK) required for outgrowth differentiation during development. In present study, we cultured NSCs...
Abstract IL-6 is not only a proinflammatory cytokine associated with inflammatory responses but also regulator on the energy and glucose metabolism in adipose tissue. Glycogen synthase kinase 3β (GSK3β) has fundamental roles regulation of pro- anti-inflammatory cytokines production. However, regulatory role for GSK3β pig response adipocytes remains unknown. We show here that SB216763 LPS increased phosphorylation (Ser9), decreased GS (Ser641) adipocytes. The activity porcine was inhibited by...
To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi.Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number crossing behavior of neurites calculated. Lentivirus was locally injected into contusion rats. functional recovery immunohistochemical staining analyzed.Neurites with exhibited significant enhancements in elongation, initiation ability when they encountered proteoglycans...
In the present study, we studied mechanism of mitochondrial ATP-sensitive potassium (mitoKATP) channels regulating hypoxia-inducible factor (HIF)-1α/microRNA (miR)-210/mitochondrial iron-sulfur protein integrin (ISCU) signaling axis and forming a positive feedback loop in chronic hypoxia-induced pulmonary arterial hypertension (PAH) by using vivo animal model. Two hundred healthy adult SPF Sprague-Dawley rats were randomly divided into five groups: Control, mimic miR-210 agent (mimic-210)...
To investigate the role of zinc finger E‑box‑binding homeobox 2 antisense RNA 1 (ZEB2-AS1) in regulating laryngeal squamous cell carcinoma (LSCC) progression.In this retrospective study, we included all patients who underwent a surgical operation at The First Hospital Qiqihaer City for LSCC. Then, compared expression ZEB2-AS1 LSCC tissues and paired healthy tissues. Besides, also performed series functional assays, CCK8 colony formation transwell assays to examine functions cells after...
Cisplatin (CDDP) is a common anticancer drug whose side effects limit its clinical applications. Tannins (TA) are plant-derived polyphenols that inhibit tumor growth in different types of cancer. Here, we evaluated the effect TA combined with CDDP on lung cancer cell lines (GLC-82 and H1299) investigated underlying molecular mechanism endoplasmic reticulum (ER) stress-induced apoptosis. Cell were treated CDDP, TA, + combination was assessed using MTT assay observed under light fluorescence...
Neural stem cells (NSCs) are characterized by their potential for self‑renewal and ability to differentiate into neurons, astrocytes, oligodendrocytes. They of great value scientific studies clinical applications. Culturing NSCs<em> in vitro</em> is important characterizing properties under controlled environmental conditions that may be modified monitored accurately. The present study explored a modified, detailed efficient protocol the isolation, culture cryopreservation rat embryonic...
Spinal cord injury (SCI) is a severe event that occurs in the central nervous system (CNS). Long noncoding RNAs (lncRNAs) have already been proven to play key roles different diseases of CNS. Nevertheless, messenger (mRNAs) and lncRNAs one week after SCI remain be elucidated. We established rat model examined differential levels mRNAs lncRNAs, then, differentially expressed (DE mRNAs) lncRNAs) were investigated. Gene Ontology (GO) functional enrichment Kyoto Encyclopedia Genes Genomes (KEGG)...
Background/purpose(s) The continuously increasing carbapenem resistance within Enterobacterales and Pseudomonas poses a threat to public health, nevertheless, the molecular characteristics of which in southern China still remain limited. And carbapenemase identification is key factor effective early therapy carbapenem-resistant bacteria infections. We aimed determine these pathogens compare commercial combined disc tests (CDTs) with modified inactivation method (mCIM) EDTA-CIM (eCIM)...
Petunia hybrida is an economically important ornamental plant species. Branching in plants closely associated with their traits, and branching a significant agronomic trait petunia, which shapes architecture production cost. Although there are few studies regarding the involvement of SPEAR genes lateral branch development, they known to regulate development organs. The PhSPEAR1 gene ortholog Arabidopsis SPEAR1, was isolated for study. According results real-time quantitative PCR (qRT-PCR),...
Tyroservatide (YSV) is an active, low-molecular-weight peptide shown to have antimetastasic effects on experimental melanoma and lung carcinoma. This study was carried out evaluate the therapeutic of YSV tumor invasion metastasis ovarian carcinoma colon explore its antitumor mechanism action. In vivo, three models were established, inhibited abdominal cavity human carcinoma, liver after spleen implantation in mice. vitro, proliferation, promoted death SKOV-3, HT-29, SW620 cells, adhesion...