A5102755241- Peptidase Inhibition and Analysis
- Postharvest Quality and Shelf Life Management
- Plant Surface Properties and Treatments
- Horticultural and Viticultural Research
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Circular RNAs in diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Drug Transport and Resistance Mechanisms
- Tannin, Tannase and Anticancer Activities
- Cancer-related Molecular Pathways
- Tea Polyphenols and Effects
- Bioactive Compounds in Plants
- PI3K/AKT/mTOR signaling in cancer
- Nanoparticle-Based Drug Delivery
- FOXO transcription factor regulation
- Phytochemistry and biological activity of medicinal plants
- Chemokine receptors and signaling
- Coconut Research and Applications
- Cancer Mechanisms and Therapy
- Pharmacological Effects of Natural Compounds
- Nuts composition and effects
Huazhong Agricultural University
2021-2023
Shanghai Zhangjiang Laboratory
2023
Citrus Research Institute
2021-2023
Ministry of Education of the People's Republic of China
2021
Tianjin Institute of Pharmaceutical Research (China)
2017-2018
Second Hospital of Tianjin Medical University
2016
Tianjin Medical University
2008-2016
Kangmei Pharmaceutical (China)
2016
// Li Ren 1, 2, * , Yang Yu Wang 2 Zhifeng Zhu Rong Lu Zhi Yao 1 Department of Clinical Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Research Center for Cancer, Key Laboratory Prevention Therapy, Tianjin’s Tianjin, P.R. China Immunology, Cellular Molecular Educational Ministry China, School Sciences, University, These authors have contributed equally to this work Correspondence to: Yao, email: yaozhi@tmu.edu.cn Keywords: CCL28, Treg, HCC, angiogenesis,...
Cancer stem cells in liver cancer are thought to be responsible for tumor recurrence and metastasis. However, the factors that mediate this mechanism have yet completely elucidated. In study, we isolated CD13+CD44+ sphere (SCs) derived from tissues SK-Hep-1 cells, which possessed cell-like properties. Through cytokine array analysis, growth differentiation factor 15 (GDF15) was significantly increased SCs. Clinical data showed GDF15 overexpressed positively related pathological grading....
Abstract A Citrus sinensis R2R3 MYB transcription factor (CsMYB96) has previously been shown to be strongly associated with the expression of many genes related wax biosynthesis in fruit. In this study, CsMYB96 was found alleviate water loss by simultaneously regulating encoding plasma membrane intrinsic proteins (CsPIPs) and wax-related genes. Expression profiling indicated that CsPIP1;1 CsPIP2;4 had high representative other aquaporins, they were down-regulated peel post-harvest citrus...
The aim of the present study was to investigate effect HIF1α on Foxp3 expression in CD4(+) CD25(-) T lymphocytes. lymphocytes were sorted from PBMC using a CD25(+) regulatory cell isolation kit. Lentivirus containing lentiviral vector that overexpressed (HIF-lenti) and those empty (control-lenti) produced. Meanwhile, lentivirus contained suppressed (siHIF-lenti) control (sicontrol-lenti) also generated. infected with HIF-lenti, control-lenti, siHIF-lenti, sicontrol-lenti, respectively....
Tyroserleutide (YSL) is an active, low-molecular-weight polypeptide, comprised of three amino acids, that has shown antitumor effects on human hepatocarcinoma BEL-7402 in vitro and vivo. In this study, we evaluated the inhibition YSL invasion adhesion mouse B16-F10 melanoma cell line by injecting cells into tail veins C57BL/6 mice to establish experimental lung metastasis model. inhibited lung, reducing number area lesions. When treated with (0.01, 0.1, 1, 10, or 100 µg/mL) vitro, found...
Tyroservatide (YSV) is an active, low-molecular weight polypeptide shown to have antitumor effects on experimental hepatocarcinoma and lung carcinoma. The focus of our study was observe the YSV several human carcinoma cell lines explore its mechanism via effect histone acetylation. Our results showed that significantly inhibited proliferation A549, NCIH460, NCIH292 NCIH1299 cells, induced G(0) /G(1) cycle arrest increased protein mRNA levels p21 p27. Moreover, treatment deacetylase (HDAC)...
It has been demonstrated that drying can significantly promote the antioxidant active substances of fresh walnut, as well enhance lipid content and conversion glycerol phospholipids to lysophospholipids in walnut. Currently, oven (OD) natural air (NAD) are two most commonly used methods for However, differences their effects on quality final product remain unclear. This study investigated effect OD NAD phenotypic physiological properties oxidation four main walnut varieties W10, W179, W185,...
Tyroserleutide (YSL) is an active, low-molecular-weight polypeptide with in vitro and vivo anticancer effects on human hepatocellular carcinoma BEL-7402 cells. In this study, we studied the of YSL PI3K/AKT cells to explore its anti-tumor mechanism. Results showed that could up-regulate mRNA protein expression tumor suppressor PTEN increase their activities, meanwhile inhibited oncogene AKT decreased kinase activities PDK1. The resuming balance effect between prevent transmission cell...
Tyroservatide (YSV) can inhibit the growth and metastasis of mouse lung cancer significantly. This study investigated therapeutic effects tripeptide YSV on human cells explored its possible mechanism that affects integrin-focal adhesion kinase (FAK) signal transduction in tumor cells. significantly inhibited invasion highly metastatic cell lines 95D, A549, NCI-H1299. In addition, phosphorylation FAK Tyr397 Tyr576/577 NCI-H1299 vitro. And mRNA level protein expression these decreased at same...
Postharvest water loss is a critical factor that determines the quality and shelf life of fresh fruit. Cuticular wax constitutes key barrier to reduce fruit loss. Our previous study has shown HJ (Citrus reticulata) significantly higher postharvest rate than ZK (Poncirus trifoliata). Here, we investigated × F1 pseudo-testcross population in 2016 2019. QTL mapping for was performed by high-density genetic map bulk segregant analysis, QTL9 identified be associated with The expression NAC042...
Abstract Background Tripeptide tyroserleutide (YSL) is a novel small molecule anti-tumor polypeptide that has been shown to inhibit the growth of human liver cancer cells. In this study, we investigated effects YSL plus doxorubicin on hepatocellular carcinoma BEL-7402 cells had transplanted into nude mice. Methods Nude mice bearing tumors were treated with successive intraperitoneal injections saline; low-, mid-, or high-dose doxorubicin; YSL. Effects weight and volume evaluated. Results...
Tyroserleutide (YSL) is a tripeptide compound that has exhibited inhibitory effects on hepatocellular carcinoma in our previous research. The mechanism of this antitumor activity involves the second messenger, Ca(2+). Ca(2+) influences cell function through Ca(2+)/calmodulin (CaM) pathway, and abnormality Ca(2+)/CaM system correlates closely with occurrence tumors. In addition, CaM associates phosphatidylinositol 3 kinase (PI3K), thereby enhancing PI3K, which promotes proliferation. order to...
Background: Tyroserleutide (YSL) inhibits the growth and metastasis of human hepatocellular carcinoma (HCC).This paper studied effect YSL on HCC investigated its mechanisms.Methods: In vivo, experimental lung models SK-HEP-1 cells in nude mice were established, vitro, proliferation, adhesion invasion detected.Results: significantly inhibited HCC.In cells.Through analyses with reverse transcription PCR (RT-PCR) Western blot, we observed that expressions ICAM-1 RT-PCR, blot zymography methods,...
The objective of this study was to develop a new small molecular peptide, tyrosyl-seryl-leucine (tyroserleutide, YSL), as an anticancer drug. Our investigated the effects YSL on human hepatocellular carcinoma and cyclin, explored its antitumor mechanism in vitro. In-vitro hepatocarcinoma cell BEL-7402 were assayed by MTS (dimethylthiazol-carboxymethoxyphenyl-sulfophenyl - tetrazolium inner salt) method. ultrastructure tumor cells observed electron microscopy. DNA ladder used investigate...
Tyroservatide (YSV) is an active, low-molecular-weight peptide shown to have antimetastasic effects on experimental melanoma and lung carcinoma. This study was carried out evaluate the therapeutic of YSV tumor invasion metastasis ovarian carcinoma colon explore its antitumor mechanism action. In vivo, three models were established, inhibited abdominal cavity human carcinoma, liver after spleen implantation in mice. vitro, proliferation, promoted death SKOV-3, HT-29, SW620 cells, adhesion...