A5100723250- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Sexual Differentiation and Disorders
- RNA Interference and Gene Delivery
- Cholesterol and Lipid Metabolism
- Peroxisome Proliferator-Activated Receptors
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- TGF-β signaling in diseases
- Renal and related cancers
- Electroconvulsive Therapy Studies
- Molecular Biology Techniques and Applications
- Acute Ischemic Stroke Management
- Tryptophan and brain disorders
- Drug Transport and Resistance Mechanisms
- Genetic Neurodegenerative Diseases
- Gastric Cancer Management and Outcomes
- Venous Thromboembolism Diagnosis and Management
- Cancer-related gene regulation
- Anesthesia and Neurotoxicity Research
Air Force Medical University
2001-2025
Wuhan University
2012-2024
Renmin Hospital of Wuhan University
2012-2024
Third Xiangya Hospital
2024
Central South University
2024
First People's Hospital of Foshan
2023
Affiliated Zhongshan Hospital of Dalian University
2022-2023
Dalian University
2023
Shandong University of Traditional Chinese Medicine
2023
First Affiliated Hospital of Xi'an Jiaotong University
2017-2023
MicroRNAs (miRNA) are a recently discovered class of noncoding RNAs that negatively regulate gene expression. Recent evidence indicates miRNAs may play an important role in cancer. However, the mechanism their deregulation neoplastic transformation has only begun to be understood. To elucidate tumor suppressor p53 regulation miRNAs, we have analyzed changes miRNA microarray expression profile immediately after conditional inactivation primary mouse ovarian surface epithelium cells. Among...
Several studies have reported that microRNA (MIR) is involved in the pathogenesis and progression of ischemic diseases, including cerebral ischemia, MIR-22 may inhibit inflammatory response cell apoptosis, which contribute to ischemia/reperfusion (I/R) injury. However, specific function I/R injury remains far from clear. This study aimed examine potential protective effect against its mechanism. As predicted, adenovirus-mediated overexpression markedly reduced neurological score infarct size...
Abstract Background Elderly patients often exhibit postoperative cognitive dysfunction (POCD), a postsurgical decline in memory and executive function. Oxidative stress neuroinflammation, both pathological characteristics of the aged brain, contribute to this decline. This study posits that electroacupuncture (EA) stimulation, an effective antioxidant anti‐inflammatory modality, may enhance telomerase reverse transcriptase (TERT) function, catalytic subunit known for its protective...
Purpose This multicenter, open-label, phase Ib/II study aimed to assess the efficacy and safety of cadonilimab, a humanized, tetravalent, bispecific antibody plus lenvatinib in first-line treatment advanced hepatocellular carcinoma (aHCC). Methods Patients with histologically confirmed aHCC were included receive either 6 mg/kg cadonilimab every 2 weeks (cohort A) or 15 3 B). The primary endpoint was objective response rate (ORR) by RECIST v1.1, while secondary endpoints safety,...
To validate whether down‐regulation of microRNA‐203 (miR‐203) in hepatocellular carcinoma (HCC) is involved HCC progression by targeting survivin. MiR‐203 mimics was transfected into HepG2 cells to enhance miR‐203 expression, and inhibitor inhibit expression. The effect up‐regulation on survivin expression evaluated using Western blot assay. or the proliferation detected CKK‐8 Over‐expression significantly inhibited ( p < 0·05), down‐expression promoted 0·05). Both over‐expression...
The present study was undertaken to investigate the regulation of P12(CDK2AP1) by miRNAs. A conserved target site for miR-21 within CDK2AP1-3'-UTR at nt 349-370 predicted bioinformatics software and an inverse correlation CDK2AP1 protein observed. Highly specific amplification quantification achieved using real-time RT-PCR. Transfection HaCaT cells with pre-miR-21 significantly suppressed a luciferase reporter including CDK2AP1-3'-UTR, whereas transfection Tca8113 anti-miR-21 increased...
MiRNA-21 was previously reported to be up-regulated in many kinds of cancer. In the present study, we want investigate potential role miRNA-21 non-small cell lung Expression detected 60 cancer (NSCLC) samples and adjacent histologically normal tissue using RT-qPCR, Correlation between expression clinicopathological features NSCLC analyzed statistical software. The effect on growth apoptosis A549 cells induced by irradiation examined. increased positively associated with lymph node...
Pancreatic cancer (PC) is one of the most fatal cancers with a very poor prognosis. Here, we found that N6-methyladenosine (m6A) RNA demethylase fat mass and obesity-related protein (FTO) promote growth, migration invasion PC. FTO expression level increased in human PC associated prognosis patients. Knockdown increases m6A methylation TFPI-2 mRNA cells, thereby increasing stability via reader YTHDF1, resulting up-regulation expression, inhibits proliferation, colony formation, sphere vitro,...
Abstract Epigenetic regulation plays an important role in cell fate reprogramming. Here, we found that inhibitors of epigenetic modifiers, including VPA, TSA, and 5‐Aza‐2'‐deoxycytidine, can induce phenotypic transformation from Jurkat cells into B‐cell‐like cells. When were treated with 5‐Aza combined B stem marker expression was observed. These gene pattern changes most remarkable the optimized induction conditions provided by cocultured genetically modified murine bone marrow OP9 In such...
Esophageal squamous cell carcinoma accounts for a large proportion of cancer-associated mortalities in both men and women. Melittin is the major active component bee venom, which has been reported to possess anti-inflammatory, antibacterial anti-cancer properties. The aim present study was construct tumor targeted recombinant plasmid [pc-telomerase reverse transcriptase (TERT)-melittin] containing human TERT promoter followed by melittin coding sequence explore effects this esophageal...
To examine how Jiang-Zhi-Ning (JZN) regulates cholesterol metabolism and compare the role of its four main components. We established a beagle model hyperlipidemia, fed with JZN extract collected JZN-containing serum 0, 1, 2, 4, 6 h later. Human liver cells Bel-7402 were stimulated 10% as well components Atorvastatin. The mRNA expression LDL receptor (LDL-R), 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMG-CoAR), cytochrome P450 7A1 (CYP7A1), acetyl-Coenzyme A acetyltransferase 2 (ACAT2) was...
Hepatocellular carcinoma (HCC) represents one of the most significant causes mortality due to cancer-related deaths. It has been previously reported that TGF-β signaling pathway may be associated with tumor progression. However, relationship between and HCC remains further elucidated. The objective our research was investigate impact on progression as well potential regulatory mechanism involved.
Abstract Exploring the role of novel cancer gene BZW1 in lung adenocarcinoma (LUAD) and unveiling associated signalling pathways. Firstly, we conducted a pan‐cancer analysis using multiple databases. Subsequently, leveraging single‐cell data from LUAD, successfully uncovered potential oncological processes with further validated them through experimentation. Simultaneously, continued to investigate molecular mechanisms underlying mediated by BZW1. Additionally, employed various machine...
Pancreatic carcinoma (PC) is a rapidly progressive, fatal malignant tumor with the poorest prognosis among all major types. MicroRNAs (miRNAs/miRs) have been indicated to be key post-transcriptional regulatory factors, which are involved in cancer development. The present study was designed investigate effect of miR-23a on PC cell proliferation, metastasis and apoptosis. expression detected normal pancreatic ductal epithelial line three lines, inhibitor or mimics were transfected into Panc-1...
The aim of this study was to determine the expression stabilities 12 common internal reference genes for relative quantitation analysis target gene performed by reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human laryngeal cancer.Hep-2 cells and 14 cancer tissue samples were investigated. characteristics candidates (18S rRNA, GAPDH, ACTB, HPRT1, RPL29, HMBS, PPIA, ALAS1, TBP, PUM1, GUSB, B2M) assessed RT-qPCR. data analyzed three commonly used software...