A5103151207- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Reproductive Biology and Fertility
- Histone Deacetylase Inhibitors Research
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- Protein Kinase Regulation and GTPase Signaling
- Genomics and Chromatin Dynamics
- Receptor Mechanisms and Signaling
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Sperm and Testicular Function
- Estrogen and related hormone effects
- 3D Printing in Biomedical Research
- HER2/EGFR in Cancer Research
- Congenital Diaphragmatic Hernia Studies
- Tissue Engineering and Regenerative Medicine
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Neuroblastoma Research and Treatments
Jiangsu University
2016-2025
Affiliated Hospital of Jiangsu University
2024-2025
Nanjing Agricultural University
2005-2009
Carbon quantum dots (CDs) are promising nanomaterials in biomedical, photocatalytical and photoelectronic applications. However, determining how to explore an ideal precursor for a renewable carbon resource is still interesting challenge. Here, the first time, we report that wastes of bagasse as new were prepared fluorescent CDs by hydrothermal carbonization (HTC) process. The characterization results show such bagasse-derived monodispersed, contain quasi spherical particles with diameter...
The initiation and progression of various solid tumors, including pancreatic carcinoma, are driven by a population cells with stem cell properties, namely cancer (CSCs). Like their normal counterparts, CSCs also believed to rely on own microenvironment termed niches sustain the population. Hypoxia-inducible factor-1α (HIF-1α) is major actor in survival response hypoxia. Recently, several researchers proposed that non-stem can convert stem-like maintain equilibrium. present study focuses...
EGFR-dependent cell migration plays an important role in lung cancer progression. Our previous study observed that the HECT E3 ubiquitin ligase NEDD4 is significantly correlated with tumor metastasis and required for invasion signaling of EGFR gastric cells. However, how promotes unknown. This to elucidate mechanism by which mediates signaling. Lentiviral vector-loaded shRNA was used deplete endogenous lines. Effects knockdown on or independent were determined using wound-healing transwell...
The MAP1LC3/LC3 family plays an essential role in autophagosomal biogenesis and transport. In this report, we show that the HECT E3 ubiquitin ligase NEDD4 interacts with LC3 is involved biogenesis. binds to through a conserved WXXL LC3-binding motif region between C2 WW2 domains. Knockdown of impaired starvation- or rapamycin-induced activation autophagy caused aggregates puncta colocalized endoplasmic reticulum membrane markers. Electron microscopy observed gigantic deformed mitochondria...
ABSTRACT Our previous studies have shown that the HECT E3 ubiquitin ligase NEDD4 interacts with LC3 and is required for starvation rapamycin-induced activation of autophagy. Here, we report directly binds to SQSTM1 via its domain polyubiquitylates SQSTM1. This ubiquitylation through K63 conjugation not involved in proteasomal degradation. Mutational analysis indicates ubiquitylates PB1 Depletion or overexpression ligase-defective mutant induced accumulation aberrant enlarged SQSTM1-positive...
Small cell lung cancer (SCLC) accounts for nearly 15% of human cancers and is one the most aggressive solid tumors. The SCLC cells are thought to derive from self-renewing pulmonary neuroendocrine by oncogenic transformation. However, whether possess stemness plasticity differentiation as normal stem has not been well understood thus far. In this study, we investigated expressions multilineage markers in line (NCI-H446) analyzed their clonogenicity, tumorigenicity, inducing differentiation....
PDZ binding-kinase (PBK) (also named T-lymphokine-activated killer cell-originated protein kinase (TOPK)), a serine/threonine kinase, is tightly controlled in normal tissues but elevated many tumors, and functions tumorigenesis metastasis. However, the signaling that regulates expression of PBK cancer cells remains elusive. Here we show atorvastatin (Lipitor), an inhibitor hydroxymethylglutaryl co-enzyme A (HMG-CoA) reductase rate-limiting enzyme mevalonate pathway, down-regulates by...
Nicotinamide mononucleotide (NMN) exerts physiological effects in mammals through its conversion to nicotinamide adenine dinucleotide (NAD+). In this study, we established experimental models of colitis by mixing drinking water C57BL/6J mice with dextran sodium sulphate (DSS), and then fed them the same concentration NMN or at time. After treatment, observed improved morphology inflamed intestines, slightly restored length colon, barrier function reduced proinflammatory factors expression...
Abstract Epigenetic abnormalities play a vital role in the progression of ovarian cancer. Lysine-specific demethylase 1 (LSD1/KDM1A) acts as an epigenetic regulator and is overexpressed tumors. However, upstream LSD1 expression this cancer remains elusive. Here, we show that epidermal growth factor (EGF) signaling upregulates protein levels SKOV3 HO8910 cells overexpressing both EGF receptor. This effect correlated with decrease dimethylation H3K4, major substrate LSD1, LSD1-dependent...
Abstract Epithelial ovarian carcinoma (EOC) is the most lethal gynecologic malignancy. However, molecular mechanisms remain unclear. In this study, we found that miR-146b was downregulated in EOC and its expression level negatively correlated with pathological staging. Follow-up functional experiments illustrated overexpression of significantly inhibited cell migration invasion, increased proliferation, but it also improved response to chemotherapeutic agents. Mechanistically, demonstrated...
Decreasing oocyte competence with maternal aging is a major factor in mammalian infertility. One of the factors contributing to this infertility changes chromatin modifications, such as histone acetylation old MII stage oocytes. Recent studies indicate that at arise germinal vesicle (GV) stage. We hypothesised methylation could also change GV To test hypothesis, we examined mono-, di- and trimethylation H3 lysine 4 (H3K4 me1, me2 me3, respectively) young older oocytes from 6-8-...
Solamargine (SM), a steroidal alkaloid glycoside extracted from the traditional Chinese herb Solanum incanum, has been evidenced to inhibit growth and induce apoptosis in number of human cancer cell lines. In present study, anticancer effect SM underlying molecular mechanism SM-induced were investigated on hepatocellular carcinoma cells, SMMC7721 HepG2. The proliferation effects HepG2 lines evaluated using MTT colony formation assays. addition, percentage was measured an Annexin V/propidium...
The aim of the present study was to determine effects insulin-like growth factor 1 (IGF-1)/phosphatase and tensin homologue deleted on chromosome 10 (PTEN)/Akt/forkhead box (FoxO) signaling pathway male reproduction in rats subjected water immersion restraint stress (WRS). Sperm morphology, sperm malformation rate, serum testosterone concentration were analyzed following WRS. In addition, expression levels immunolocalization IGF‑1, PTEN, Akt FoxO proteins, as well rate cell apoptosis rat...
Epithelial ovarian cancer (EOC) is the most lethal gynecological malignancy. LIN28 homolog A (LIN28A) a RNA‑binding protein, which serves fundamental role in cell development and pluripotency. Polo‑like kinase 4 (PLK4) member of polo‑like family, primarily takes part mitotic regulation. Overexpression LIN28A has been demonstrated cancer; however, expression PLK4 correlation between EOC not discussed. In present study, mRNA protein levels were evaluated by reverse transcription‑quantitative...