A5064126122- Hepatitis B Virus Studies
- Signaling Pathways in Disease
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Renal Transplantation Outcomes and Treatments
- Viral Infections and Immunology Research
- Cardiovascular Function and Risk Factors
- Cardiac Imaging and Diagnostics
- Liver Diseases and Immunity
- Coronary Interventions and Diagnostics
- Diabetes Management and Research
- Cardiac Valve Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Drug-Induced Hepatotoxicity and Protection
- Ion channel regulation and function
- Immune Cell Function and Interaction
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Monoclonal and Polyclonal Antibodies Research
- Pharmacological Effects and Toxicity Studies
- Immunodeficiency and Autoimmune Disorders
Iwate Medical University
2025
Toho University
2024-2025
Shinshu University
1996-2024
Hokkaido University
2018-2024
Hamamatsu Medical Center
2009-2023
Fujita Health University
2021-2023
Fujita Health University Okazaki Medical Center
2021-2023
Tohoku University Hospital
2017-2022
Nagasaki Medical Center
2012-2021
Fujita Health University Hospital
2021
The predictive role of antinuclear antibodies (ANAs) remains elusive in the long-term outcome primary biliary cirrhosis (PBC). progression PBC was evaluated association with ANAs using stepwise Cox proportional hazard regression and an unconditional logistic model based on data 276 biopsy-proven, definite patients who have been registered to National Hospital Organization Study Group for Liver Disease Japan (NHOSLJ). When death hepatic failure/liver transplantation (LT) defined as end-point,...
The PRMT5•MTA complex has recently emerged as a new synthetically lethal drug target for the treatment of
The mycelia of the edible mushroom Lentinula edodes can be cultured in solid medium containing lignin, and hot-water extracts (L.E.M.) is commercially available as a nutritional supplement. During cultivation, phenolic compounds, such syringic acid vanillic acid, were produced by lignin-degrading peroxidase secreted from L. mycelia. Since these compounds have radical scavenging activity, we examined their protective effect on oxidative stress mice with CCl(4)-induced liver injury. We...
The edible mushroom Lentinula edodes (shiitake) contains many bioactive compounds. In the present study, we cultivated L. mycelia in solid medium and examined hot-water extract (L.E.M.) for its suppressive effect on concanavalin A (ConA)-induced liver injury mice. ConA injection into tail vein caused a great increase serum aspartate aminotransferase (AST) alanine (ALT) levels. intraperitoneal administration of L.E.M. significantly decreased levels transaminases. substances, including...
This study was conducted to clarify if the long-term histological outcome among patients with chronic hepatitis C differs according whether they are infected genotype 1 or 2 virus (HCV). We examined 140 C. The HCV determined by enzyme-linked immunosorbent assay (ELISA) based on genotypes and specific recombinant proteins; found in 100 (96 were 1b 4 indeterminate) 36. two groups showed no significant difference for any clinical background features. Deterioration of grade liver histology...
Macrocyclization improves the pharmaceutical properties of peptides; however, regio- and chemoselective intramolecular cyclizations remain challenging. Here we developed a streamlined chemoenzymatic approach to synthesize cyclic peptides by exploiting non-ribosomal peptide (NRP) cyclases. Linear linked resin through C-terminal diol ester functionality are synthesized on solid support, circumvent installation leaving groups peptidic substrates in liquid phase which often triggers undesirable...
Abstract Bicyclic peptides exhibit improved metabolic stabilities and target specificities when compared to their linear or mono-cyclic counterparts; however, efficient straightforward synthesis remains challenging due intricate architectures. Here, we present a highly selective operationally simple one-pot chemoenzymatic tandem cyclization approach synthesize bicyclic with small medium ring sizes. Penicillin-binding protein-type thioesterases (PBP-type TEs) efficiently cyclized...
Summary Background Pemphigus is an antidesmoglein (Dsg) autoimmune disease that divided into two major subtypes: pemphigus foliaceus (PF) and vulgaris (PV). We previously developed enzyme‐linked immunosorbent assays (ELISAs) using recombinant Dsg1 Dsg3 to detect IgG autoantibodies in patients with pemphigus. The protocol for the ELISAs was optimized serological diagnosis, but under conditions used, these were not particularly useful monitoring activity certain patients. That is, sera from...
To investigate the presence of beta3-adrenoceptor (beta3-AR) in human and rat detrusor muscle usefulness beta3-AR agonists as drugs for treatment urinary frequency.FK175, ethyl [(S)-8-[(R)-2-(3-chlorophenyl)-2-hydroxyethylamino]-6,7,8,9-tetrah ydro-5H-benzocyclohepton-2-yloxy]acetate monohydrochloride monohydrate, was used a selective agonist. The expression beta-AR subtypes (beta1-, beta2-, beta3-AR) mRNA investigated by RT-PCR. Beta3-AR agonist induced cyclic AMP (cAMP) levels were...
This study was conducted to clarify if the long-term histological outcome among patients with chronic hepatitis C differs according whether they are infected genotype 1 or 2 virus (HCV). We examined 140 C. The HCV determined by enzyme-linked immunosorbent assay (ELISA) based on genotypes and specific recombinant proteins; found in 100 (96 were 1b 4 indeterminate) 36. two groups showed no significant difference for any clinical background features. Deterioration of grade liver histology...
NOD mice with knockout of both native insulin genes and a mutated proinsulin transgene, alanine at position B16 in preproinsulin (B16:A-dKO mice), do not develop diabetes. Transplantation islets, but bone marrow, expressing sequences (tyrosine B16) into B16:A-dKO rapidly restored development autoantibodies (IAAs) insulitis, despite the recipients' pancreatic islets lacking sequences. Splenocytes from that received insulin–positive induced diabetes when transferred wild-type NOD/SCID or mice....
Abstract The insect neuropeptide family FXPRLa, which carries the Phe-Xaa-Pro-Arg-Leu-NH 2 sequence at C-terminus, is involved in many physiological processes. Although ligand–receptor interactions FXPRLa signaling have been examined using vitro assays, correlation between these and vivo function unclear. Diapause silkworm, Bombyx mori , thought to be elicited by diapause hormone (DH, an FXPRLa) signaling, consists of DH receptor (DHR). Here, we performed transcription activator-like...
Significance Diapause is a common response to seasonal changes in environmental conditions found many species of insects, as well other animals, but we do not yet fully understand the molecular mechanisms underlying this important physiological switch. Although there has been previous research on diapause, remain questions regarding diapause determination signals. Our highlighted presence GABAergic and corazonin signaling system that induces progeny through hormone release. findings also...
<h3>ABSTRACT</h3> <h3>BACKGROUND AND PURPOSE:</h3> Post-carotid endarterectomy (CEA) cerebral hyperperfusion (CH) can cause intracerebral hemorrhage and cognitive decline. Alterations in susceptibility response to acetazolamide (ACZ) on 7T MRI quantitative mapping (QSM) detects elevated CBV occurring due impaired cerebrovascular autoregulation. We explored preoperative relative changes QSM ACZ their ability predict CH following CEA. <h3>MATERIALS METHODS:</h3> Sixty-three patients with...
Abstract We report a Pd‐catalyzed oxidative Pictet‐Spengler reaction/aromatization cascade utilizing readily available benzylic alcohols for the construction of 1‐aryl‐ β ‐carbolines in water. Conceptually, this novel one‐pot, three‐step approach involves an initial Pd(0)/sodium diphenylphosphinobenzene‐3‐sulfonate (TPPMS)‐catalyzed dehydrogenation alcohols, followed by cyclization situ generated aldehydes with tryptamines, and concludes DBU/air‐promoted aromatization, allowing rapid access...
ABSTRACT Membrane proteins (MPs) are important yet challenging targets for drug discovery. MPs can be reconstituted in protein‐lipid Nanodiscs (NDs), which resemble the native membrane environment. Drug‐membrane interactions affect apparent binding stoichiometry and affinity, as well kinetics of ligands a particular target, is extrapolation to pharmacokinetic studies. To investigate role membrane, we have applied fragment‐based discovery (FBDD) methods cytochrome P450 3A4 (CYP3A4), NDs...
We characterized type 3 ryanodine receptor (RyR3) purified from rabbit diaphragm by immunoaffinity chromatography using a specific antibody. The was free 12-kDa FK506-binding protein, although it retained the ability to bind protein. Negatively stained images of RyR3 show characteristic rectangular structure that indistinguishable RyR1. location D2 segment, which exists uniquely in RyR1 isoform, determined as region around domain 9 close corner square-shaped assembly, with use D2-directed...
AimsTo examine the relation between mitochondrial dysfunction and myocardial contractile relaxation reserves in hypertrophic cardiomyopathy (HCM).
Glycogenic hepatopathy (GH) has been reported as a very rare and under recognized complication in long-standing poorly controlled type 1 diabetes (T1D) patients. GH is characterized by transient elevation of liver transaminase hepatomegaly caused reversible excessive glycogen accumulation hepatocytes. It that indistinguishable from non-alcoholic fatty disease, which more commonly seen diabetic patients, even after history taken physical examination or imaging studies have performed. can only...