A5018956141- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Ion Channels and Receptors
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Neuroscience and Neural Engineering
- Cancer-related molecular mechanisms research
- Nicotinic Acetylcholine Receptors Study
- Neuropeptides and Animal Physiology
- Hereditary Neurological Disorders
- COVID-19 diagnosis using AI
- Cardiac electrophysiology and arrhythmias
- Adenosine and Purinergic Signaling
- Phytochemicals and Antioxidant Activities
- Photoreceptor and optogenetics research
- Exercise and Physiological Responses
- Axon Guidance and Neuronal Signaling
- Thermal Regulation in Medicine
- RNA modifications and cancer
- CNS Lymphoma Diagnosis and Treatment
- Toxin Mechanisms and Immunotoxins
- Neurogenesis and neuroplasticity mechanisms
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
Wenzhou Medical University
2023-2025
Affiliated Eye Hospital of Wenzhou Medical College
2023-2025
Shenzhen University
2025
Shenzhen Institutes of Advanced Technology
2024
Drexel University
2012-2024
Rutgers, The State University of New Jersey
2018-2024
Rutgers New Jersey Medical School
2003-2024
Wenzhou Hospital of Traditional Chinese Medicine
2024
Wuhan University
2014-2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Protein kinase C (PKC) modulates the function of capsaicin receptor transient potential vanilloid 1 (TRPV1). This modulation manifests as increased current when channel is activated by capsaicin. In addition, studies have suggested that phosphorylation PKC might directly gate channel, because PKC-activating phorbol esters induce TRPV1 currents in absence applied ligands. To test whether both and gates direct phosphorylation, we used sequencing to determine major sites on intracellular...
Neuropathic pain resulting from nerve lesions or dysfunction represents one of the most challenging neurological diseases to treat. A better understanding molecular mechanisms responsible for causing these maladaptive responses can help develop novel therapeutic strategies and biomarkers neuropathic pain. We performed a miRNA expression profiling study dorsal root ganglion (DRG) tissue rats four weeks post spinal ligation (SNL), model TaqMan low density arrays identified 63 miRNAs whose...
The prevalence and levels of Listeria monocytogenes in retail raw foods covering most provincial capitals China were studied with testing 1036 samples vegetables, edible mushrooms, meat, aquatic products quick-frozen from September 2012 to January 2014. total was 20.0% (207/1036), the probable number (MPN) values 65.7% positive ranged 0.3 110 MPN/g. Geographical differences observed this survey, results both qualitative quantitative methods indicated that North higher than those South China....
Nerve injury-induced maladaptive changes of gene expression in dorsal root ganglion (DRG) neurons contribute to neuropathic pain. Long non-coding RNAs (lncRNAs) are emerging as key regulators expression. Here, a conserved lncRNA is reported, named DRG-specifically enriched (DS-lncRNA) for its high DRG neurons. Peripheral nerve injury downregulates DS-lncRNA injured due, part, silencing POU domain, class 4, transcription factor 3, that interacts with the promoter. Rescuing downregulation...
In addition to its role as a CNS neurotransmitter, glutamate has been shown recently be an important component of the peripheral inflammation response. We demonstrated previously that group I metabotropic receptors (mGluRs) mGlu1 and mGlu5 are expressed in terminals sensory neurons activation mGluRs skin increases thermal sensitivity. present study, we provide evidence suggesting increase sensitivity by enhancing vanilloid (capsaicin) receptor function. show potentiates capsaicin responses...
The transient outward potassium currents (also known as A-type or I A ) are important determinants of neuronal excitability. In the brain, is modulated by protein kinase C (PKC), (PKA), and extracellular signal-related (ERK), three kinases that have been shown to be critical modulators nociception. We wanted determine effects these on in superficial dorsal horn neurons. Using whole cell recordings from cultured mouse spinal cord neurons, we found PKC PKA both inhibit cells, has a tonic...
Protein kinases belonging to the protein kinase A (PKA), C (PKC), and extracellular signal-related (ERK) families have been identified as key players in modulating nociception at level of spinal cord dorsal horn, yet little is known about effects these on membrane properties horn neurons. PKA, PKC, ERK exert inhibitory transient potassium currents (A-type or I ) mouse superficial neurons ( Hu et al. 2003 ). Here we aimed determine action potential firing evaluate impact modulation (and other...
Metabotropic glutamate receptors (mGluRs) play important roles in the modulation of nociception. The group I mGluRs (mGlu1 and mGlu5) modulate nociceptive plasticity via activation extracellular signal-regulated kinase (ERK) signaling. We reported recently that K + channel Kv4.2 subunit underlies A-type currents spinal cord dorsal horn is modulated by ERK signaling pathway. Kv4.2-mediated are determinants neuronal excitability central sensitization hypersensitivity after tissue injury. In...
Menthol is one of the most commonly used chemicals in our daily life, not only because its fresh flavor and cooling feeling but also medical benefit. Previous studies have suggested that menthol produces analgesic action acute neuropathic pain through peripheral mechanisms. However, central actions mechanisms remain unclear. Here, we report has direct effects on spinal cord. decreased both ipsilateral contralateral hypersensitivity induced by complete Freund9s adjuvant a dose-dependent...
ATP can activate a variety of pathways through P2 purinoreceptors, leading to neuroprotection and pathology in the CNS. Among all P2X receptors, P2X7 receptor (P2X7R) is well-defined therapeutic target for inflammatory neuropathic pain. Activation P2X7R generate reactive oxygen species (ROS) macrophages microglia. However, role ROS P2X7R-induced pain remains unexplored. Here, we investigated downstream effects neuronal activation spinal cord. We found that induces production cord dorsal...
Abstract Nerve injury to peripheral somatosensory system causes refractory neuropathic pain. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis this disorder. Long non-coding RNAs (lncRNAs) key regulators transcription; however, their significance pain remains largely elusive.Here, we reported a novel lncRNA, named neuron-specific lncRNA (SS-lncRNA), for its exclusively dorsal root ganglion (DRG) and trigeminal ganglion. SS-lncRNA was...
Store-operated calcium channels (SOCs) are highly calcium-selective that mediate entry in various cell types. We have previously reported intraplantar injection of YM-58483 (a SOC inhibitor) attenuates chronic pain. A previous study has the function SOCs dorsal root ganglia (DRG) is enhanced after nerve injury, suggesting may play a peripheral role However, expression, functional distribution and significance family DRG neurons remain elusive key components (SOCE) still controversial. Here,...
Transmembrane Ca v 2.2 (N-type) voltage-gated calcium channels are genetically and pharmacologically validated, clinically relevant pain targets. Clinical block of (e.g., with Prialt/Ziconotide) or indirect modulation [e.g., gabapentinoids such as Gabapentin (GBP)] mitigates chronic but is encumbered by side effects abuse liability. The cytosolic auxiliary subunit collapsin response mediator protein 2 (CRMP2) targets to the sensory neuron membrane regulates their function via an...
Cancer patients undergoing treatment with antineoplastic drugs often experience chemotherapy-induced neuropathic pain (CINP), and the therapeutic options for managing CINP are limited. Here, we show that systemic paclitaxel administration upregulates expression of neurotrophin-3 (Nt3) mRNA NT3 protein in neurons dorsal root ganglia (DRG), but not spinal cord. Blocking upregulation attenuates paclitaxel-induced mechanical, heat, cold nociceptive hypersensitivities spontaneous without altering...
CPAMD8, a constituent of the A2M/C3 (α-2-macroglobulin/complement 3) protein family, is strikingly expressed in human fetal lens and distal neural retina. Mutations CPAMD8 have been linked to anterior segment dysgenesis primary congenital glaucoma. We utilized CRISPR/Cas9 technology establish homozygous knockout embryonic stem cell line for differentiating retinal organoids, with intent exploring role early development eye. The exhibits normal morphology, pluripotency, karyotype, serving as...
Our previous study demonstrated that a store-operated calcium channel (SOCC) inhibitor (YM-58483) has central analgesic effects. However, the cellular and molecular mechanisms of such effects remain to be determined. It is well-known glial cells play important roles in sensitization. SOC entry (SOCE) been implicated many cell types including cortical astrocytes. role SOCC family function astrocytes not Here, we thoroughly investigated expression functional significance SOCCs spinal Primary...
Voltage-dependent Na + channels are usually expressed in neurons that use spikes as a means of signal coding. Retinal bipolar cells commonly thought to be nonspiking neurons, category the CNS uses graded potential for transmission. Here we report first time voltage-dependent currents acutely isolated mammalian retinal with whole cell patch-clamp recordings. were observed ∼45% recorded cone but not rod cells. Both on and off found express channels. The activated at membrane potentials around...
Chronic pain often accompanies immune responses and cells are known to be involved in chronic pain. Store-operated calcium (SOC) channels calcium-selective cation play an important role the system. YM-58483, a potent SOC channel inhibitor, has been shown inhibit cytokine production from attenuate antigen-induced hypersensitivity reactions. Here, we report that YM-58483 analgesic actions produces antinociceptive effects acute prevents development of mice. Oral administration 10mg/kg or 30...
Store-operated calcium channels (SOCs) are calcium-selective cation that mediate entry in many different cell types. (SOCE) is involved various cellular functions. Increasing evidence suggests impairment of SOCE responsible for numerous disorders. A previous study demonstrated YM-58483, a potent SOC inhibitor, strongly attenuates chronic pain by systemic or intrathecal injection and completely blocks the second phase formalin-induced spontaneous nocifensive behaviour, suggesting potential...