A5101644980- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Head and Neck Cancer Studies
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Mechanisms of cancer metastasis
- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Digestive system and related health
- Adipokines, Inflammation, and Metabolic Diseases
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- 14-3-3 protein interactions
- Cardiovascular Disease and Adiposity
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Cancer Mechanisms and Therapy
- Aortic Disease and Treatment Approaches
- Metabolism, Diabetes, and Cancer
- Enzyme Structure and Function
- Regulation of Appetite and Obesity
- Lipid metabolism and disorders
- Circular RNAs in diseases
Central South University
2013-2024
Xiangya Hospital Central South University
2012-2024
Weatherford College
2022
Third Hospital of Changsha
2011
Radiotherapy is the primary treatment for nasopharyngeal cancer (NPC), but radioresistance remains a serious obstacle to successful in many cases. To identify proteins involved this resistance and evaluate their potential predicting NPC response radiotherapy, we first established radioresistant subclone cell line (CNE2-IR) derived from CNE2 by treating cells with five rounds of sublethal ionizing radiation. Proteomics was then performed compare protein profiles CNE2-IR CNE2, total 34...
Abstract Chromatin modification is pivotal to the epithelial–mesenchymal transition (EMT), which confers potent metastatic potential cancer cells. Here, we report a role for chromatin remodeling factor lymphoid-specific helicase (LSH) in nasopharyngeal carcinoma (NPC), prevalent China. LSH expression was increased NPC, where it controlled by Epstein–Barr virus-encoded protein LMP1. In NPC cells vitro and vivo, promoted progression part regulating of fumarate hydratase (FH), core component...
Abstract Cholangiocytes play a crucial role in bile formation. Cholangiocyte injury causes cholestasis, including primary biliary cholangitis (PBC). However, the etiology of PBC remains unclear despite being characterized as an autoimmune disease. Using single-cell RNA sequencing (scRNA-seq), fluorescence-activated-cell-sorting, multiplex immunofluorescence (IF) and RNAscope analyses, we identified unique DUOX2 + ACE2 small cholangiocytes human mouse livers. Their selective decrease patients...
Chemoresistance is a major barrier to successful cisplatinbased chemotherapy for epithelial ovarian cancer (EOC), and emerging evidences suggest that microRNAs (miRNAs) are involved in the resistance. In this study, it was indicated miR-363 downregulation significantly correlated with EOC carcinogenesis cisplatin Moreover, overexpression could resensitise cisplatin-resistant cells treatment both vitro vivo. addition, data revealed EMT inducer Snail upregulated cell lines patients functional...
Abstract Most cancer patients receive radiotherapy in the course of their disease and occurrence radioresistance is associated with poor prognosis. The molecular pathways that drive enhanced tumorigenic potential during development are poorly understood. Here, we demonstrate aryl hydrocarbon receptor (AhR) plays a vital role maintenance stem-like properties. AhR promotes phenotype drives metastasis by directly targeting promoters ‘stemness’ genes, such as ATP-binding cassette sub-family G...
Abstract 14‐3‐3 σ, the downstream target of p53, is a negative regulator cell cycle G2‐M phase checkpoint in response to DNA damage. Our previous comparative proteomics study showed that σ was downregulated or lost nasopharyngeal carcinoma (NPC) tissue compared with non‐cancerous epithelial (NNET). In this study, we further investigated for epigenetic mechanism inactivation. Methylation‐specific PCR promoter methylation 100% analyzed NPC lines (4/4) but not immortalized human line NP69....
Abstract Angiogenesis is critical for re‐establishing the blood supply to surviving myocardium after myocardial infarction ( MI ) in patients with acute coronary syndrome ACS ). Micro RNA s are recognised as important epigenetic regulators of endothelial function. The aim this study was determine roles micro angiogenesis. Eighteen circulating including miR‐185‐5p were differently expressed plasma from by high‐throughput sequencing. expressional levels dramatically reduced hearts isolated...
Postoperative acute kidney injury (pAKI) is a serious complication of Stanford type A aortic dissection (TAAD) surgery, which significantly associated with the inflammatory response. This study aimed to explore relationship between blood count-derived markers (BCDIMs) and pAKI construct predictive model for pAKI. Patients who underwent TAAD surgery were obtained from our center Medical Information Mart Intensive Care (MIMIC)-IV database. The differences in preoperative BCDIMs clinical...
Abstract Raf kinase inhibitory protein (RKIP) is a metastasis suppressor whose expression reduced in nasopharyngeal carcinoma (NPC) tissues and absent NPC metastases. To investigate the effect of RKIP on radiosensitivity NPC, high metastatic 5‐8F with low non‐metastatic 6‐10B were stably transfected plasmids that expressed sense antisense cDNA. Overexpression sensitized cells to radiation‐induced cell death, G 2 ‐M cycle arrest apoptosis. In contrast, downexpression protected from addition,...
Atrial fibrillation (AF) is related to structural and electrical atria remodeling. fibrosis development progression characteristic of remodeling taken as the AF perpetuation substrate. Increasing evidence has confirmed that microRNAs (miRNAs) are associated with AF, including cardiac fibrosis.Pericardial fluid (PF) samples were collected from nine adult patients who had congenital heart disease persistent or sinus rhythm (SR) undergoing surgery. Abnormally expressed miRNAs acquired, P<0.05...
The epidermal growth factor receptor (EGFR) is usually overexpressed in nasopharyngeal carcinoma (NPC) and associated with pathogenesis of NPC. However, while EGFR-modulated intracellular proteins have been extensively studied, little known concerning their extracellular counterparts. To identify EGFR-regulated secreted NPC, we compared the secretome profiles TGF-α-stimulated unstimulated NPC cell line CNE-2. CNE-2 cells were cultured absence or presence TGF-α for 24 h, obtained from...
Objective: This study aimed to explore whether eukaryotic translation elongation factor 1 alpha 2 affected cell proliferation, migration, and apoptosis via regulating the dimethylation of at lysine 55 in acute myeloid leukemia. Methods: The expressions leukemia lines human normal bone marrow mononuclear cells (as control) were assessed. Control CRISPR-Cas9 lentivirus, knockout vector plasmid, wild type overexpression with a K55R substitution plasmid transfected into AML-193 Kasumi-1 combined...
Objective . To investigate the differential protein profile of human lung squamous carcinoma (HLSC) and normal bronchial epithelium (NBE) provide preliminary results for further study to explore carcinogenic mechanism HLSC. Methods Laser capture microdissection (LCM) was used purify target cells from 10 pairs HLSC tissues their matched NHBE, respectively. A stable-isotope labeled strategy using iTRAQ, followed by 2D-LC/Q-STAR mass spectrometry, performed separate identify expression...
To investigate osteopontin (OPN) expression in human nasopharyngeal carcinoma (NPC) and evaluate its clinical significance the disease.The of OPN mRNA 44 frozen NPC tissue 15 normal epithelium (NNET) samples was examined by semi-quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). protein 67 paraffin-embedded 21 NNET detected immunohistochemistry (IHC). In addition, investigated 12 paired para-carcinoma (PCT) western blotting (WB). The association between clinicopathologic...
Objective The mortality of type A aortic dissection (TAAD) is extremely high. effect postoperative hyperglycemia (PHG) on the prognosis TAAD surgery unclear. This study aims to investigate patients with PHG after and construct prediction model for PHG. Methods Patients underwent from January 2016 December 2020 in Xiangya Hospital were collected. total 203 included divided into non group group. occurrence delirium, cardiac complications, spinal cord complication, cerebral acute kidney injury...
Metastasis in lung cancer portends a poor prognosis, and the epithelial-mesenchymal transition (EMT) cells is considered prerequisite to achieve metastatic potential. Recent studies indicate that BTB/POZ domain-containing protein 7 (BTBD7) regulates EMT-associated proteins human malignancies however, role of BTBD7 have not been identified. In present study, we examined expression status its association with unfavorable clinical features non-small-cell (NSCLC). Firstly, studied fresh...
Background: Atrial fibrillation (AF) increases the risk of stroke and heart failure. Postoperative AF (POAF) mortality after cardiac surgery. This study aims to explore mechanisms underlying AF, analyze infiltration immune cells in left atrium (LA) from patients with identify potential circular RNA (circRNA) biomarkers for POAF. Methods: Raw data GSE797689, GSE115574, GSE97455 were downloaded processed. AF-related gene co-expression network was constructed using weighted correlation analysis...
The fusion gene encoding the thyroid-specific transcription factor PAX8 and peroxisome proliferator-activated receptor γ (PPARγ (PPARG)) (designated as PPFP gene) is oncogenic implicated in development of follicular thyroid carcinoma (FTC). effects transfection on biological characteristics Nthy-ori 3-1 cells were studied by MTT assay, colony formation, soft-agar scratch wound-healing assays well flow cytometry. Furthermore, differentially expressed proteins analyzed 2-DE maps identified...