A5104727766- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Immune responses and vaccinations
- Immune Response and Inflammation
- Microbial infections and disease research
- Veterinary Oncology Research
- Infectious Diseases and Tuberculosis
- Immunodeficiency and Autoimmune Disorders
- vaccines and immunoinformatics approaches
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Pancreatitis Pathology and Treatment
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Gut microbiota and health
- Bartonella species infections research
- Virus-based gene therapy research
- Insect Pheromone Research and Control
- Bacteriophages and microbial interactions
- Immune cells in cancer
- Evolution and Paleontology Studies
- Vaccine Coverage and Hesitancy
- Drug Transport and Resistance Mechanisms
- Polyomavirus and related diseases
- SARS-CoV-2 and COVID-19 Research
- Analytical Chemistry and Chromatography
Office National de la Chasse et de la Faune Sauvage
2020-2023
Animal and Plant Health Agency
2011-2022
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2020-2022
Modèles Insectes de l'Immunité Innée
2022
Veterinary Medicines Directorate
2003-2009
Chembio (United States)
2009
University College Dublin
2005-2008
Understanding the pathogenesis of SARS-CoV-2 infection is key to developing preventive and therapeutic strategies against COVID-19, in case severe illness but also when disease mild. The use appropriate experimental animal models remains central vivo exploration physiopathology antiviral strategies. This study describes intranasal ferrets hamsters with low doses low-passage clinical French isolate UCN19, describing levels, excretion, immune responses pathological patterns both species....
ABSTRACT It is estimated that more than 50 million cattle are infected with Mycobacterium bovis worldwide, resulting in severe economic losses. Current diagnosis of tuberculosis (TB) relies on tuberculin skin testing, and when combined the slaughter test-positive animals, it has significantly reduced incidence bovine TB. The failure to eradicate TB Great Britain been attributed part a reservoir infection badgers ( Meles meles ). Accurate reliable cornerstone control. Bacteriological these...
The European badger (Meles meles) is a reservoir host of Mycobacterium bovis and responsible for proportion the tuberculosis (TB) cases seen in cattle United Kingdom Republic Ireland. An injectable preparation bacillus Calmette-Guérin (BCG) vaccine licensed use badgers UK its forms part bovine TB eradication plans England Wales. However, there are practical limitations to widespread application an research priority development oral deliverable bait. Previous studies reported successful...
Oral vaccination with Mycobacterium bovis Bacille of Calmette and Guerin (BCG) has provided protection against M. to badgers both experimentally in the field. There is also evidence suggesting that persistence live BCG within host important for maintaining TB. Here we investigated capacity badger inductive mucosal sites absorb maintain BCG. The targeted mucosae were oropharyngeal cavity (tonsils sublingual area) small intestine (ileum). We showed significant quantities persisted tissues...
Bovine tuberculosis (bTB) caused by Mycobacterium bovis is the most serious endemic disease affecting livestock in UK. The European badger (Meles meles) important wildlife reservoir of bTB transmission to cattle, making eradication particularly difficult. In this respect, oral vaccination with attenuated M. vaccine Bacillus Calmette-Guerin (BCG) has been suggested as a wide-scale intervention reduce infection badgers. However, experimental studies show variable protection. Among...
Badgers (Meles meles) are a major tuberculosis (TB) reservoir in Europe, with the potential to transmit infection cattle. Here we assessed whether recently described oral vaccine based on heat-inactivated Mycobacterium bovis (HIMB), delivered as edible baits, can protect badgers from infection. Eight were given individually five each one consisting of ball peanut butter, natural and oat flakes including dose containing 5 × 107 colony-forming units. In parallel, control group seven did not...
A real-time PCR assay for the measurement of gamma interferon (IFN-gamma) mRNA in European badger (Meles meles) blood cultures was developed. The levels IFN-gamma stimulated with either bovine or avian tuberculin specific mycobacterial antigens were compared those a nonstimulated control culture as basis determining tuberculosis (TB) status live badgers. validated by testing 247 animals which there matching data from postmortem examination and tissues. Relative changes response to found be...
ABSTRACT Deer are acknowledged as hosts of Mycobacterium bovis , the causative agent bovine tuberculosis (bTB), and determining prevalence infection in deer species is one key steps understanding epidemiological role played by cervids transmission maintenance bTB United Kingdom. This study evaluated a rapid lateral-flow test for detection samples from wild Fallow ( Dama dama ), roe Capreolus capreolus red Cervus elaphus ) areas Wales, Cotswolds, southwestern England were necropsied survey....
Polyomaviruses infect a diverse range of mammalian and avian hosts, are associated with variety symptoms. However, it is unknown whether the viruses found in all families evolutionary history polyomaviruses still unclear. Here, we report discovery novel polyomavirus European badger (Meles meles), which to our knowledge represents first be characterized family Mustelidae, within carnivoran. Although virus was discovered serendipitously supernatant cell culture inoculated material,...
The aim of this study was to assess the frequency mustelid herpesvirus-1 (MusHV-1) infection in free-ranging badgers (Meles meles) British Isles. A polymerase chain reaction assay developed that detected MusHV-1 DNA 95% (18/19) and 100% (10/10) anticoagulant-treated blood samples collected from sampled southwest England Republic Ireland, respectively. An indirect immunoassay also detect MusHV-1–specific immunoglobulin-G serum samples. Using an arbitrary cutoff twice optical density obtained...
Bovine tuberculosis (TB) in Great Britain adversely affects animal health and welfare is a cause of considerable economic loss. The situation exacerbated by European badgers (Meles meles) acting as wildlife source recurrent Mycobacterium bovis infection to cattle. Vaccination against TB possible means reduce control bovine TB. delivery vaccine oral bait holds the best prospect for vaccinating over wide geographical area. There are practical limitations volume concentration Bacillus Calmette...
In developing an oral bait BCG vaccine against tuberculosis in badgers we wanted to understand the conditions of gastrointestinal tract and their impact on viability. Conditions mimicking stomach small-intestine caused substantial reduction We performed vivo experiments using a telemetric pH monitoring system used data parameterise dynamic vitro (TIM-1) small intestine. Some died compartment through duodenum jejunum compartments. survival was greatest when absent but by time reached jejunum,...
Tuberculosis (TB) vaccination could be used as a key part of integrated strategies for the disease’s control if an effective and safe vaccine under field conditions is obtained. Recent studies in Spain have evaluated protective efficacy two oral vaccines against experimental challenge with live intra-bronchial Mycobacterium bovis captive badgers: live-attenuated M. BCG (Danish strain) heat-inactivated (HIMB) vaccine. With objective increasing knowledge cellular development progress infection...
An oral vaccine is a potential tool to tackle the reservoir of Mycobacterium bovis in European badgers (Meles meles), which contributes tuberculosis cattle British Isles. Inferences about protection against experimental challenge with M. depend on measurement tuberculosis. Assessment larger species, such as badgers, typically based tuberculous lesions visible at post-mortem examination and histopathology. We have developed robust scoring system for by combining several parallel measures, we...